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Article: Clostridium difficile isolates with increased sporulation: Emergence of PCR ribotype 002 in Hong Kong

TitleClostridium difficile isolates with increased sporulation: Emergence of PCR ribotype 002 in Hong Kong
Authors
KeywordsBiomedicine
Medical Microbiology
Internal Medicine
Issue Date2011
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/10096/index.htm
Citation
European Journal Of Clinical Microbiology And Infectious Diseases, 2011, v. 30 n. 11, p. 1371-1381 How to Cite?
AbstractWe identified a predominant clone of Clostridium difficile PCR ribotype 002, which was associated with an increased sporulation frequency. In 2009, 3,528 stool samples from 2,440 patients were tested for toxigenic C. difficile in a healthcare region in Hong Kong. A total of 345 toxigenic strains from 307 (13.3%) patients were found. Ribotype 002 was the predominant ribotype, which constituted 35 samples from 29 (9.4%) patients. The mean sporulation frequency of ribotype 002 was 20.2%, which was significantly higher than that of the 56 randomly selected ribotypes other than 002 as concurrent controls (3.7%, p<0.001). Patients carrying toxigenic ribotype 002 were more frequently admitted from an elderly home (p=0.01) and received more β-lactam antibiotics in the preceding 3 months compared with the controls (p=0.04) . The identification of toxigenic ribotype 002 in 2009 was temporally related to a significant increase in both the incidence of toxigenic C. difficile from 0.53 to 0.95 per 1,000 admissions (p<0.001) and the rate of positive detection from 4.17% to 6.28% (p<0.001) between period 1 (2004-2008) and period 2 (2009). This finding should alert both the physician and the infection control team to the establishment of and possible outbreaks by ribotype 002 in our hospitals, as in the case of ribotype 027. © 2011 The Author(s).
Persistent Identifierhttp://hdl.handle.net/10722/144876
ISSN
2014 Impact Factor: 2.668
2014 SCImago Journal Rankings: 1.037
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
Food and Health Bureau (FHB) of the Hong Kong SAR Government
Funding Information:

We thank Dr. E.J. Kuijper (Leiden University, the Netherlands) for providing us the standard strains of C. difficile PCR ribotypes used as the positive control. The work was supported by the Research Fund for the Control of Infectious Diseases (RFCID) of the Food and Health Bureau (FHB) of the Hong Kong SAR Government.

References

 

DC FieldValueLanguage
dc.contributor.authorCheng, VCCen_HK
dc.contributor.authorYam, WCen_HK
dc.contributor.authorLam, OTCen_HK
dc.contributor.authorTsang, JLYen_HK
dc.contributor.authorTse, EYFen_HK
dc.contributor.authorSiu, GKHen_HK
dc.contributor.authorChan, JFWen_HK
dc.contributor.authorTse, Hen_HK
dc.contributor.authorTo, KKWen_HK
dc.contributor.authorTai, JWMen_HK
dc.contributor.authorHo, PLen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2012-02-21T05:43:38Z-
dc.date.available2012-02-21T05:43:38Z-
dc.date.issued2011en_HK
dc.identifier.citationEuropean Journal Of Clinical Microbiology And Infectious Diseases, 2011, v. 30 n. 11, p. 1371-1381en_HK
dc.identifier.issn0934-9723en_HK
dc.identifier.urihttp://hdl.handle.net/10722/144876-
dc.description.abstractWe identified a predominant clone of Clostridium difficile PCR ribotype 002, which was associated with an increased sporulation frequency. In 2009, 3,528 stool samples from 2,440 patients were tested for toxigenic C. difficile in a healthcare region in Hong Kong. A total of 345 toxigenic strains from 307 (13.3%) patients were found. Ribotype 002 was the predominant ribotype, which constituted 35 samples from 29 (9.4%) patients. The mean sporulation frequency of ribotype 002 was 20.2%, which was significantly higher than that of the 56 randomly selected ribotypes other than 002 as concurrent controls (3.7%, p<0.001). Patients carrying toxigenic ribotype 002 were more frequently admitted from an elderly home (p=0.01) and received more β-lactam antibiotics in the preceding 3 months compared with the controls (p=0.04) . The identification of toxigenic ribotype 002 in 2009 was temporally related to a significant increase in both the incidence of toxigenic C. difficile from 0.53 to 0.95 per 1,000 admissions (p<0.001) and the rate of positive detection from 4.17% to 6.28% (p<0.001) between period 1 (2004-2008) and period 2 (2009). This finding should alert both the physician and the infection control team to the establishment of and possible outbreaks by ribotype 002 in our hospitals, as in the case of ribotype 027. © 2011 The Author(s).en_HK
dc.languageengen_US
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/10096/index.htmen_HK
dc.relation.ispartofEuropean Journal of Clinical Microbiology and Infectious Diseasesen_HK
dc.rightsThe Author(s)en_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong Licenseen_US
dc.subjectBiomedicineen_US
dc.subjectMedical Microbiologyen_US
dc.subjectInternal Medicineen_US
dc.titleClostridium difficile isolates with increased sporulation: Emergence of PCR ribotype 002 in Hong Kongen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4551/resserv?sid=springerlink&genre=article&atitle=<i>Clostridium difficile</i> isolates with increased sporulation: emergence of PCR ribotype 002 in Hong Kong&title=European Journal of Clinical Microbiology & Infectious Diseases&issn=09349723&date=2011-11-01&volume=30&issue=11& spage=1371&authors=V. C. C. Cheng, W. C. Yam, O. T. C. Lam, <i>et al.</i>en_US
dc.identifier.emailYam, WC: wcyam@hkucc.hku.hken_HK
dc.identifier.emailChan, JFW: jfwchan@hku.hken_HK
dc.identifier.emailTse, H: htse@hkucc.hku.hken_HK
dc.identifier.emailTo, KKW: kelvinto@hkucc.hku.hken_HK
dc.identifier.emailHo, PL: plho@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityYam, WC=rp00313en_HK
dc.identifier.authorityChan, JFW=rp01736en_HK
dc.identifier.authorityTse, H=rp00519en_HK
dc.identifier.authorityTo, KKW=rp01384en_HK
dc.identifier.authorityHo, PL=rp00406en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturepublished_or_final_versionen_US
dc.identifier.doi10.1007/s10096-011-1231-0en_HK
dc.identifier.pmid21468685-
dc.identifier.pmcidPMC3191290-
dc.identifier.scopuseid_2-s2.0-80054757852en_HK
dc.identifier.hkuros190822-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80054757852&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume30en_HK
dc.identifier.issue11en_HK
dc.identifier.spage1371en_HK
dc.identifier.epage1381en_HK
dc.identifier.eissn1435-4373en_US
dc.identifier.isiWOS:000295864800009-
dc.publisher.placeGermanyen_HK
dc.description.otherSpringer Open Choice, 21 Feb 2012en_US
dc.identifier.scopusauthoridCheng, VCC=38662328400en_HK
dc.identifier.scopusauthoridYam, WC=7004281720en_HK
dc.identifier.scopusauthoridLam, OTC=54385361900en_HK
dc.identifier.scopusauthoridTsang, JLY=54386386400en_HK
dc.identifier.scopusauthoridTse, EYF=37065312200en_HK
dc.identifier.scopusauthoridSiu, GKH=35485473100en_HK
dc.identifier.scopusauthoridChan, JFW=24278817900en_HK
dc.identifier.scopusauthoridTse, H=7006070596en_HK
dc.identifier.scopusauthoridTo, KKW=14323807300en_HK
dc.identifier.scopusauthoridTai, JWM=7101993154en_HK
dc.identifier.scopusauthoridHo, PL=7402211363en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK
dc.identifier.citeulike9144412-

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