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Article: Optimization of fixation methods for observation of bacterial cell morphology and surface ultrastructures by atomic force microscopy

TitleOptimization of fixation methods for observation of bacterial cell morphology and surface ultrastructures by atomic force microscopy
Authors
KeywordsAtomic force microscopy
Cell morphology
Fixation methods
Ultrastructure
Issue Date2011
PublisherSpringer. The Journal's web site is located at http://link.springer.de/link/service/journals/00253/index.htm
Citation
Applied Microbiology And Biotechnology, 2011, v. 92 n. 2, p. 381-392 How to Cite?
Abstract
Fixation ability of five common fixation solutions, including 2.5% glutaraldehyde, 10% formalin, 4% paraformaldehyde, methanol/acetone (1:1), and ethanol/acetic acid (3:1) were evaluated by using atomic forcemicroscopy in the present study. Three model bacteria, i.e., Escherichia coli, Pseudomonas putida, and Bacillus subtilis were applied to observe the above fixation methods for the morphology preservation of bacterial cells and surface ultrastructures. All the fixation methods could effectively preserve cell morphology. However, for preserving bacterial surface ultrastructures, the methods applying aldehyde fixations performed much better than those using alcohols, since the alcohols could detach the surface filaments (i.e., flagella and pili) significantly. Based on the quantitative and qualitative assessments, the 2.5% glutaraldehyde was proposed as a promising fixation solution both for observing morphology of both bacterial cell and surface ultrastructures, while the methonal/acetone mixture was the worst fixation solution which may obtain unreliable results. © The Author(s) 2011. © Springer-Verlag 2011.
Persistent Identifierhttp://hdl.handle.net/10722/144871
ISSN
2013 Impact Factor: 3.811
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong UGCSEG HKU10
University of Hong Kong
Funding Information:

The authors wish to thank the Hong Kong UGC One-off Special Equipment Grant Scheme (SEG HKU10) for the financial support on this study, and Yuanqing Chao wishes to thank the University of Hong Kong for the postgraduate studentship. The technical assistance of Ms. Vicky Fung is greatly appreciated.

References

 

Author Affiliations
  1. The University of Hong Kong
DC FieldValueLanguage
dc.contributor.authorChao, Yen_HK
dc.contributor.authorZhang, Ten_HK
dc.date.accessioned2012-02-21T05:43:45Z-
dc.date.available2012-02-21T05:43:45Z-
dc.date.issued2011en_HK
dc.identifier.citationApplied Microbiology And Biotechnology, 2011, v. 92 n. 2, p. 381-392en_HK
dc.identifier.issn0175-7598en_HK
dc.identifier.urihttp://hdl.handle.net/10722/144871-
dc.description.abstractFixation ability of five common fixation solutions, including 2.5% glutaraldehyde, 10% formalin, 4% paraformaldehyde, methanol/acetone (1:1), and ethanol/acetic acid (3:1) were evaluated by using atomic forcemicroscopy in the present study. Three model bacteria, i.e., Escherichia coli, Pseudomonas putida, and Bacillus subtilis were applied to observe the above fixation methods for the morphology preservation of bacterial cells and surface ultrastructures. All the fixation methods could effectively preserve cell morphology. However, for preserving bacterial surface ultrastructures, the methods applying aldehyde fixations performed much better than those using alcohols, since the alcohols could detach the surface filaments (i.e., flagella and pili) significantly. Based on the quantitative and qualitative assessments, the 2.5% glutaraldehyde was proposed as a promising fixation solution both for observing morphology of both bacterial cell and surface ultrastructures, while the methonal/acetone mixture was the worst fixation solution which may obtain unreliable results. © The Author(s) 2011. © Springer-Verlag 2011.en_HK
dc.languageEngen_US
dc.publisherSpringer. The Journal's web site is located at http://link.springer.de/link/service/journals/00253/index.htmen_HK
dc.relation.ispartofApplied Microbiology and Biotechnologyen_HK
dc.rightsThe Author(s)en_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong Licenseen_US
dc.subjectAtomic force microscopyen_HK
dc.subjectCell morphologyen_HK
dc.subjectFixation methodsen_HK
dc.subjectUltrastructureen_HK
dc.titleOptimization of fixation methods for observation of bacterial cell morphology and surface ultrastructures by atomic force microscopyen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4551/resserv?sid=springerlink&genre=article&atitle=Optimization of fixation methods for observation of bacterial cell morphology and surface ultrastructures by atomic force microscopy&title=Applied Microbiology and Biotechnology&issn=01757598&date=2011-10-01&volume=92&issue=2& spage=381&authors=Yuanqing Chao, Tong Zhangen_US
dc.identifier.emailZhang, T:zhangt@hkucc.hku.hken_HK
dc.identifier.authorityZhang, T=rp00211en_HK
dc.description.naturepublished_or_final_versionen_US
dc.identifier.doi10.1007/s00253-011-3551-5en_HK
dc.identifier.pmid21881891en_HK
dc.identifier.pmcidPMC3181414-
dc.identifier.scopuseid_2-s2.0-82455164106en_HK
dc.identifier.hkuros208086en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-82455164106&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume92en_HK
dc.identifier.issue2en_HK
dc.identifier.spage381en_HK
dc.identifier.epage392en_HK
dc.identifier.eissn1432-0614en_US
dc.identifier.isiWOS:000295325300015-
dc.publisher.placeGermanyen_HK
dc.description.otherSpringer Open Choice, 21 Feb 2012en_US
dc.identifier.scopusauthoridChao, Y=36503486900en_HK
dc.identifier.scopusauthoridZhang, T=24470677400en_HK
dc.identifier.citeulike9765282-

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