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Article: Optimization of fixation methods for observation of bacterial cell morphology and surface ultrastructures by atomic force microscopy
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TitleOptimization of fixation methods for observation of bacterial cell morphology and surface ultrastructures by atomic force microscopy
 
AuthorsChao, Y1
Zhang, T1
 
KeywordsAtomic force microscopy
Cell morphology
Fixation methods
Ultrastructure
 
Issue Date2011
 
PublisherSpringer. The Journal's web site is located at http://link.springer.de/link/service/journals/00253/index.htm
 
CitationApplied Microbiology And Biotechnology, 2011, v. 92 n. 2, p. 381-392 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s00253-011-3551-5
 
AbstractFixation ability of five common fixation solutions, including 2.5% glutaraldehyde, 10% formalin, 4% paraformaldehyde, methanol/acetone (1:1), and ethanol/acetic acid (3:1) were evaluated by using atomic forcemicroscopy in the present study. Three model bacteria, i.e., Escherichia coli, Pseudomonas putida, and Bacillus subtilis were applied to observe the above fixation methods for the morphology preservation of bacterial cells and surface ultrastructures. All the fixation methods could effectively preserve cell morphology. However, for preserving bacterial surface ultrastructures, the methods applying aldehyde fixations performed much better than those using alcohols, since the alcohols could detach the surface filaments (i.e., flagella and pili) significantly. Based on the quantitative and qualitative assessments, the 2.5% glutaraldehyde was proposed as a promising fixation solution both for observing morphology of both bacterial cell and surface ultrastructures, while the methonal/acetone mixture was the worst fixation solution which may obtain unreliable results. © The Author(s) 2011. © Springer-Verlag 2011.
 
ISSN0175-7598
2012 Impact Factor: 3.689
2012 SCImago Journal Rankings: 1.307
 
DOIhttp://dx.doi.org/10.1007/s00253-011-3551-5
 
PubMed Central IDPMC3181414
 
ISI Accession Number IDWOS:000295325300015
Funding AgencyGrant Number
Hong Kong UGCSEG HKU10
University of Hong Kong
Funding Information:

The authors wish to thank the Hong Kong UGC One-off Special Equipment Grant Scheme (SEG HKU10) for the financial support on this study, and Yuanqing Chao wishes to thank the University of Hong Kong for the postgraduate studentship. The technical assistance of Ms. Vicky Fung is greatly appreciated.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorChao, Y
 
dc.contributor.authorZhang, T
 
dc.date.accessioned2012-02-21T05:43:45Z
 
dc.date.available2012-02-21T05:43:45Z
 
dc.date.issued2011
 
dc.description.abstractFixation ability of five common fixation solutions, including 2.5% glutaraldehyde, 10% formalin, 4% paraformaldehyde, methanol/acetone (1:1), and ethanol/acetic acid (3:1) were evaluated by using atomic forcemicroscopy in the present study. Three model bacteria, i.e., Escherichia coli, Pseudomonas putida, and Bacillus subtilis were applied to observe the above fixation methods for the morphology preservation of bacterial cells and surface ultrastructures. All the fixation methods could effectively preserve cell morphology. However, for preserving bacterial surface ultrastructures, the methods applying aldehyde fixations performed much better than those using alcohols, since the alcohols could detach the surface filaments (i.e., flagella and pili) significantly. Based on the quantitative and qualitative assessments, the 2.5% glutaraldehyde was proposed as a promising fixation solution both for observing morphology of both bacterial cell and surface ultrastructures, while the methonal/acetone mixture was the worst fixation solution which may obtain unreliable results. © The Author(s) 2011. © Springer-Verlag 2011.
 
dc.description.naturepublished_or_final_version
 
dc.description.otherSpringer Open Choice, 21 Feb 2012
 
dc.identifier.citationApplied Microbiology And Biotechnology, 2011, v. 92 n. 2, p. 381-392 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s00253-011-3551-5
 
dc.identifier.citeulike9765282
 
dc.identifier.doihttp://dx.doi.org/10.1007/s00253-011-3551-5
 
dc.identifier.eissn1432-0614
 
dc.identifier.epage392
 
dc.identifier.hkuros208086
 
dc.identifier.isiWOS:000295325300015
Funding AgencyGrant Number
Hong Kong UGCSEG HKU10
University of Hong Kong
Funding Information:

The authors wish to thank the Hong Kong UGC One-off Special Equipment Grant Scheme (SEG HKU10) for the financial support on this study, and Yuanqing Chao wishes to thank the University of Hong Kong for the postgraduate studentship. The technical assistance of Ms. Vicky Fung is greatly appreciated.

 
dc.identifier.issn0175-7598
2012 Impact Factor: 3.689
2012 SCImago Journal Rankings: 1.307
 
dc.identifier.issue2
 
dc.identifier.openurl
 
dc.identifier.pmcidPMC3181414
 
dc.identifier.pmid21881891
 
dc.identifier.scopuseid_2-s2.0-82455164106
 
dc.identifier.spage381
 
dc.identifier.urihttp://hdl.handle.net/10722/144871
 
dc.identifier.volume92
 
dc.languageEng
 
dc.publisherSpringer. The Journal's web site is located at http://link.springer.de/link/service/journals/00253/index.htm
 
dc.publisher.placeGermany
 
dc.relation.ispartofApplied Microbiology and Biotechnology
 
dc.relation.referencesReferences in Scopus
 
dc.rightsThe Author(s)
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.subjectAtomic force microscopy
 
dc.subjectCell morphology
 
dc.subjectFixation methods
 
dc.subjectUltrastructure
 
dc.titleOptimization of fixation methods for observation of bacterial cell morphology and surface ultrastructures by atomic force microscopy
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong