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Conference Paper: Autocrine/paracrine regulation of somatolactin {alpha} production in carp pituitary cells by local release of somatolactin {alpha} and {beta}: functional role of Jak2/Stat5, PI3K/Akt and MAPK signaling cascades
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TitleAutocrine/paracrine regulation of somatolactin {alpha} production in carp pituitary cells by local release of somatolactin {alpha} and {beta}: functional role of Jak2/Stat5, PI3K/Akt and MAPK signaling cascades
 
AuthorsJiang, Q
Wong, AOL
 
Issue Date2011
 
PublisherThe Endocrine Society. The Journal's web site is located at http://edrv.endojournals.org
 
CitationThe 93rd Annual Meeting & Expo of the Endocrine Spciety (ENDO 2011), Boston, MA., 4-7 June 2011. In Endocrine Reviews, 2011, v. 32 n. 3, Meeting abstracts, abstract no. P1-401 [How to Cite?]
 
AbstractPituitary hormones can act locally via autocrine/paracrine mechanisms to modulate pituitary functions, which represent an interesting aspect of pituitary regulation other than the traditional hypothalamic control and feedback signals from peripheral tissues. Somatolactin (SL), a member of the growth hormone/prolactin family, is a pleiotropic hormone with diverse functions in fish models, but its pituitary actions have not been previously examined. Recently, two SL isoforms, SLα and SLβ, have been cloned in grass carp. Based on the sequences obtained, recombinant proteins of grass carp SLα and SLβ with bioactivity in inducing pigment aggregation in carp melanophores were produced. In carp pituitary cells, SLα secretion and cellular content were elevated by static incubation with carp SLα and SLβ, respectively. These stimulatory actions occurred with a parallel raise in SLα mRNA levels with no significant changes in SLβ protein content and gene expression. In parallel experiments, SLα mRNA expression could be reduced by removing endogenous SLα and SLβ using immunoneutralization with the respective SL antisera. At the pituitary cell level, SLα mRNA expression induced by carp SLα and SLβ could be blocked by inhibiting JAK2, STAT5, PI3K, Akt, MEK1/2, and P38 MAPK, respectively. Furthermore, SLα and SLβ treatment could also trigger rapid phosphorylation of STAT5b, Akt, MEK1/2, Erk1/2, MKK3, and P38 MAPK. These results, as a whole, suggest that (i) SLα and SLβ produced locally in the carp pituitary can serve as novel autocrine/paracrine stimulators for SLα release and synthesis, and (ii) SLα production caused by SLα and SLβ stimulation probably are mediated by SLα gene expression via activation of the JAK2/STAT5, PI3K/Akt and MAPK signaling pathways.
 
ISSN0163-769X
2012 Impact Factor: 14.873
2012 SCImago Journal Rankings: 5.997
 
DC FieldValue
dc.contributor.authorJiang, Q
 
dc.contributor.authorWong, AOL
 
dc.date.accessioned2012-02-13T04:49:31Z
 
dc.date.available2012-02-13T04:49:31Z
 
dc.date.issued2011
 
dc.description.abstractPituitary hormones can act locally via autocrine/paracrine mechanisms to modulate pituitary functions, which represent an interesting aspect of pituitary regulation other than the traditional hypothalamic control and feedback signals from peripheral tissues. Somatolactin (SL), a member of the growth hormone/prolactin family, is a pleiotropic hormone with diverse functions in fish models, but its pituitary actions have not been previously examined. Recently, two SL isoforms, SLα and SLβ, have been cloned in grass carp. Based on the sequences obtained, recombinant proteins of grass carp SLα and SLβ with bioactivity in inducing pigment aggregation in carp melanophores were produced. In carp pituitary cells, SLα secretion and cellular content were elevated by static incubation with carp SLα and SLβ, respectively. These stimulatory actions occurred with a parallel raise in SLα mRNA levels with no significant changes in SLβ protein content and gene expression. In parallel experiments, SLα mRNA expression could be reduced by removing endogenous SLα and SLβ using immunoneutralization with the respective SL antisera. At the pituitary cell level, SLα mRNA expression induced by carp SLα and SLβ could be blocked by inhibiting JAK2, STAT5, PI3K, Akt, MEK1/2, and P38 MAPK, respectively. Furthermore, SLα and SLβ treatment could also trigger rapid phosphorylation of STAT5b, Akt, MEK1/2, Erk1/2, MKK3, and P38 MAPK. These results, as a whole, suggest that (i) SLα and SLβ produced locally in the carp pituitary can serve as novel autocrine/paracrine stimulators for SLα release and synthesis, and (ii) SLα production caused by SLα and SLβ stimulation probably are mediated by SLα gene expression via activation of the JAK2/STAT5, PI3K/Akt and MAPK signaling pathways.
 
dc.description.otherThe 93rd Annual Meeting & Expo of the Endocrine Spciety (ENDO 2011), Boston, MA., 4-7 June 2011. In Endocrine Reviews, 2011, v. 32 n. 3, Meeting abstracts, abstract no. P1-401
 
dc.identifier.citationThe 93rd Annual Meeting & Expo of the Endocrine Spciety (ENDO 2011), Boston, MA., 4-7 June 2011. In Endocrine Reviews, 2011, v. 32 n. 3, Meeting abstracts, abstract no. P1-401 [How to Cite?]
 
dc.identifier.hkuros196998
 
dc.identifier.issn0163-769X
2012 Impact Factor: 14.873
2012 SCImago Journal Rankings: 5.997
 
dc.identifier.issue3, Meeting abstracts
 
dc.identifier.urihttp://hdl.handle.net/10722/144842
 
dc.identifier.volume32
 
dc.languageeng
 
dc.publisherThe Endocrine Society. The Journal's web site is located at http://edrv.endojournals.org
 
dc.relation.ispartofEndocrine Reviews
 
dc.titleAutocrine/paracrine regulation of somatolactin {alpha} production in carp pituitary cells by local release of somatolactin {alpha} and {beta}: functional role of Jak2/Stat5, PI3K/Akt and MAPK signaling cascades
 
dc.typeConference_Paper
 
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<date.issued>2011</date.issued>
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<description.abstract>Pituitary hormones can act locally via autocrine/paracrine mechanisms to modulate pituitary functions, which represent an interesting aspect of pituitary regulation other than the traditional hypothalamic control and feedback signals from peripheral tissues. Somatolactin (SL), a member of the growth hormone/prolactin family, is a pleiotropic hormone with diverse functions in fish models, but its pituitary actions have not been previously examined. Recently, two SL isoforms, SL&#945; and SL&#946;, have been cloned in grass carp. Based on the sequences obtained, recombinant proteins of grass carp SL&#945; and SL&#946; with bioactivity in inducing pigment aggregation in carp melanophores were produced. In carp pituitary cells, SL&#945; secretion and cellular content were elevated by static incubation with carp SL&#945; and SL&#946;, respectively. These stimulatory actions occurred with a parallel raise in SL&#945; mRNA levels with no significant changes in SL&#946; protein content and gene expression. In parallel experiments, SL&#945; mRNA expression could be reduced by removing endogenous SL&#945; and SL&#946; using immunoneutralization with the respective SL antisera. At the pituitary cell level, SL&#945; mRNA expression induced by carp SL&#945; and SL&#946; could be blocked by inhibiting JAK2, STAT5, PI3K, Akt, MEK1/2, and P38 MAPK, respectively. Furthermore, SL&#945; and SL&#946; treatment could also trigger rapid phosphorylation of STAT5b, Akt, MEK1/2, Erk1/2, MKK3, and P38 MAPK. These results, as a whole, suggest that (i) SL&#945; and SL&#946; produced locally in the carp pituitary can serve as novel autocrine/paracrine stimulators for SL&#945; release and synthesis, and (ii) SL&#945; production caused by SL&#945; and SL&#946; stimulation probably are mediated by SL&#945; gene expression via activation of the JAK2/STAT5, PI3K/Akt and MAPK signaling pathways.</description.abstract>
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