Article: Characterization of chicken secretin (SCT) and secretin receptor (SCTR) genes: A novel secretin-like peptide (SCT-LP) and secretin encoded in a single gene
| Title | Characterization of chicken secretin (SCT) and secretin receptor (SCTR) genes: A novel secretin-like peptide (SCT-LP) and secretin encoded in a single gene | ||||
|---|---|---|---|---|---|
| Authors | Wang, Y1 Huang, G1 Li, J1 Meng, F1 He, X1 Leung, FC2 | ||||
| Keywords | Chicken Pancreas SCTR Secretin Secretin-like peptide | ||||
| Issue Date | 2012 | ||||
| Publisher | Elsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/mce | ||||
| Citation | Molecular And Cellular Endocrinology, 2012, v. 348 n. 1, p. 270-280 [How to Cite?] DOI: http://dx.doi.org/10.1016/j.mce.2011.09.012 | ||||
| Abstract | Secretin and the secretin receptor have been reported to play an important role in regulating pancreatic water and bicarbonate secretion in mammals; however, little is known about their expression, structure, and biological functions in non-mammalian vertebrates including birds. In this study, the full-length cDNAs encoding secretin and secretin receptor have first been cloned from duodenum of adult chickens. The putative chicken secretin receptor (cSCTR) is 449 amino acids in length and shares high sequence identity (58-63%) with its mammalian counterparts. Interestingly, chicken secretin cDNA encodes not only the secretin peptide (cSCT), but also a novel secretin-like peptide (cSCT-LP), which shares high amino acid identity with chicken (56%) and mammalian (48-52%) secretin. Using a pGL3-CRE-luciferase reporter system, we further demonstrated that both cSCT (EC 50: 0.31nM) and cSCT-LP (EC 50: 1.10nM), but not other structurally-related peptides, could potently activate cSCTR expressed in CHO cells, suggesting that both peptides may function as potential ligands for cSCTR. Using RT-PCR, the expression of secretin and secretin receptor in adult chicken tissues was also examined. Secretin was detected to be predominantly expressed in small intestine, while the mRNA expression of cSCTR was restricted to several tissues including gastrointestinal tract, liver, testis, pancreas and several brain regions. Collectively, results from present study not only established a molecular basis to elucidate the physiological roles of SCT, SCT-LP and SCTR in chickens, but also provide critical insights into structural and functional changes of secretin and its receptor during vertebrate evolution. © 2011 Elsevier Ireland Ltd. | ||||
| ISSN | 0303-7207 2011 Impact Factor: 4.192 2011 SCImago Journal Rankings: 0.375 | ||||
| DOI | http://dx.doi.org/10.1016/j.mce.2011.09.012 | ||||
| ISI Accession Number ID | WOS:000297539400031
Funding Information: This work was supported by grants from the National Natural Science Foundation of China (30771171, 30871338, 30971569). Special thanks are given to Professor M.E. El-halwani (Department of Animal Science, University of Minnesota, Saint Paul, MN) for providing us with chicken VIP and turkey PHI. We would also like to give special thanks to the two anonymous reviewers for their constructive suggestions for the publication of this manuscript. | ||||
| References | References in Scopus |
| dc.contributor.author | Wang, Y | ||||
|---|---|---|---|---|---|
| dc.contributor.author | Huang, G | ||||
| dc.contributor.author | Li, J | ||||
| dc.contributor.author | Meng, F | ||||
| dc.contributor.author | He, X | ||||
| dc.contributor.author | Leung, FC | ||||
| dc.date.accessioned | 2012-02-03T06:20:10Z | ||||
| dc.date.available | 2012-02-03T06:20:10Z | ||||
| dc.date.issued | 2012 | ||||
| dc.description.abstract | Secretin and the secretin receptor have been reported to play an important role in regulating pancreatic water and bicarbonate secretion in mammals; however, little is known about their expression, structure, and biological functions in non-mammalian vertebrates including birds. In this study, the full-length cDNAs encoding secretin and secretin receptor have first been cloned from duodenum of adult chickens. The putative chicken secretin receptor (cSCTR) is 449 amino acids in length and shares high sequence identity (58-63%) with its mammalian counterparts. Interestingly, chicken secretin cDNA encodes not only the secretin peptide (cSCT), but also a novel secretin-like peptide (cSCT-LP), which shares high amino acid identity with chicken (56%) and mammalian (48-52%) secretin. Using a pGL3-CRE-luciferase reporter system, we further demonstrated that both cSCT (EC 50: 0.31nM) and cSCT-LP (EC 50: 1.10nM), but not other structurally-related peptides, could potently activate cSCTR expressed in CHO cells, suggesting that both peptides may function as potential ligands for cSCTR. Using RT-PCR, the expression of secretin and secretin receptor in adult chicken tissues was also examined. Secretin was detected to be predominantly expressed in small intestine, while the mRNA expression of cSCTR was restricted to several tissues including gastrointestinal tract, liver, testis, pancreas and several brain regions. Collectively, results from present study not only established a molecular basis to elucidate the physiological roles of SCT, SCT-LP and SCTR in chickens, but also provide critical insights into structural and functional changes of secretin and its receptor during vertebrate evolution. © 2011 Elsevier Ireland Ltd. | ||||
| dc.description.nature | Link_to_subscribed_fulltext | ||||
| dc.identifier.citation | Molecular And Cellular Endocrinology, 2012, v. 348 n. 1, p. 270-280 [How to Cite?] DOI: http://dx.doi.org/10.1016/j.mce.2011.09.012 | ||||
| dc.identifier.citeulike | 9835125 | ||||
| dc.identifier.doi | http://dx.doi.org/10.1016/j.mce.2011.09.012 | ||||
| dc.identifier.epage | 280 | ||||
| dc.identifier.hkuros | 198297 | ||||
| dc.identifier.isi | WOS:000297539400031
Funding Information: This work was supported by grants from the National Natural Science Foundation of China (30771171, 30871338, 30971569). Special thanks are given to Professor M.E. El-halwani (Department of Animal Science, University of Minnesota, Saint Paul, MN) for providing us with chicken VIP and turkey PHI. We would also like to give special thanks to the two anonymous reviewers for their constructive suggestions for the publication of this manuscript. | ||||
| dc.identifier.issn | 0303-7207 2011 Impact Factor: 4.192 2011 SCImago Journal Rankings: 0.375 | ||||
| dc.identifier.issue | 1 | ||||
| dc.identifier.pmid | 21939730 | ||||
| dc.identifier.scopus | eid_2-s2.0-80755132149 | ||||
| dc.identifier.spage | 270 | ||||
| dc.identifier.uri | http://hdl.handle.net/10722/144728 | ||||
| dc.identifier.volume | 348 | ||||
| dc.language | eng | ||||
| dc.publisher | Elsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/mce | ||||
| dc.publisher.place | Ireland | ||||
| dc.relation.ispartof | Molecular and Cellular Endocrinology | ||||
| dc.relation.references | References in Scopus | ||||
| dc.subject.mesh | Amino Acid Sequence | ||||
| dc.subject.mesh | Cyclic AMP-Dependent Protein Kinases - metabolism | ||||
| dc.subject.mesh | Receptors, G-Protein-Coupled - genetics - metabolism | ||||
| dc.subject.mesh | Receptors, Gastrointestinal Hormone - genetics - metabolism | ||||
| dc.subject.mesh | Secretin - genetics - metabolism - pharmacology | ||||
| dc.subject | Chicken | ||||
| dc.subject | Pancreas | ||||
| dc.subject | SCTR | ||||
| dc.subject | Secretin | ||||
| dc.subject | Secretin-like peptide | ||||
| dc.title | Characterization of chicken secretin (SCT) and secretin receptor (SCTR) genes: A novel secretin-like peptide (SCT-LP) and secretin encoded in a single gene | ||||
| dc.type | Article |
Author Affiliations
- Sichuan University
- The University of Hong Kong