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Article: Characterization of chicken secretin (SCT) and secretin receptor (SCTR) genes: A novel secretin-like peptide (SCT-LP) and secretin encoded in a single gene

TitleCharacterization of chicken secretin (SCT) and secretin receptor (SCTR) genes: A novel secretin-like peptide (SCT-LP) and secretin encoded in a single gene
Authors
KeywordsChicken
Pancreas
SCTR
Secretin
Secretin-like peptide
Issue Date2012
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/mce
Citation
Molecular And Cellular Endocrinology, 2012, v. 348 n. 1, p. 270-280 How to Cite?
Abstract
Secretin and the secretin receptor have been reported to play an important role in regulating pancreatic water and bicarbonate secretion in mammals; however, little is known about their expression, structure, and biological functions in non-mammalian vertebrates including birds. In this study, the full-length cDNAs encoding secretin and secretin receptor have first been cloned from duodenum of adult chickens. The putative chicken secretin receptor (cSCTR) is 449 amino acids in length and shares high sequence identity (58-63%) with its mammalian counterparts. Interestingly, chicken secretin cDNA encodes not only the secretin peptide (cSCT), but also a novel secretin-like peptide (cSCT-LP), which shares high amino acid identity with chicken (56%) and mammalian (48-52%) secretin. Using a pGL3-CRE-luciferase reporter system, we further demonstrated that both cSCT (EC 50: 0.31nM) and cSCT-LP (EC 50: 1.10nM), but not other structurally-related peptides, could potently activate cSCTR expressed in CHO cells, suggesting that both peptides may function as potential ligands for cSCTR. Using RT-PCR, the expression of secretin and secretin receptor in adult chicken tissues was also examined. Secretin was detected to be predominantly expressed in small intestine, while the mRNA expression of cSCTR was restricted to several tissues including gastrointestinal tract, liver, testis, pancreas and several brain regions. Collectively, results from present study not only established a molecular basis to elucidate the physiological roles of SCT, SCT-LP and SCTR in chickens, but also provide critical insights into structural and functional changes of secretin and its receptor during vertebrate evolution. © 2011 Elsevier Ireland Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/144728
ISSN
2013 Impact Factor: 4.241
2013 SCImago Journal Rankings: 2.044
ISI Accession Number ID
Funding AgencyGrant Number
National Natural Science Foundation of China30771171
30871338
30971569
Funding Information:

This work was supported by grants from the National Natural Science Foundation of China (30771171, 30871338, 30971569). Special thanks are given to Professor M.E. El-halwani (Department of Animal Science, University of Minnesota, Saint Paul, MN) for providing us with chicken VIP and turkey PHI. We would also like to give special thanks to the two anonymous reviewers for their constructive suggestions for the publication of this manuscript.

References

 

Author Affiliations
  1. The University of Hong Kong
  2. Sichuan University
DC FieldValueLanguage
dc.contributor.authorWang, Yen_HK
dc.contributor.authorHuang, Gen_HK
dc.contributor.authorLi, Jen_HK
dc.contributor.authorMeng, Fen_HK
dc.contributor.authorHe, Xen_HK
dc.contributor.authorLeung, FCen_HK
dc.date.accessioned2012-02-03T06:20:10Z-
dc.date.available2012-02-03T06:20:10Z-
dc.date.issued2012en_HK
dc.identifier.citationMolecular And Cellular Endocrinology, 2012, v. 348 n. 1, p. 270-280en_HK
dc.identifier.issn0303-7207en_HK
dc.identifier.urihttp://hdl.handle.net/10722/144728-
dc.description.abstractSecretin and the secretin receptor have been reported to play an important role in regulating pancreatic water and bicarbonate secretion in mammals; however, little is known about their expression, structure, and biological functions in non-mammalian vertebrates including birds. In this study, the full-length cDNAs encoding secretin and secretin receptor have first been cloned from duodenum of adult chickens. The putative chicken secretin receptor (cSCTR) is 449 amino acids in length and shares high sequence identity (58-63%) with its mammalian counterparts. Interestingly, chicken secretin cDNA encodes not only the secretin peptide (cSCT), but also a novel secretin-like peptide (cSCT-LP), which shares high amino acid identity with chicken (56%) and mammalian (48-52%) secretin. Using a pGL3-CRE-luciferase reporter system, we further demonstrated that both cSCT (EC 50: 0.31nM) and cSCT-LP (EC 50: 1.10nM), but not other structurally-related peptides, could potently activate cSCTR expressed in CHO cells, suggesting that both peptides may function as potential ligands for cSCTR. Using RT-PCR, the expression of secretin and secretin receptor in adult chicken tissues was also examined. Secretin was detected to be predominantly expressed in small intestine, while the mRNA expression of cSCTR was restricted to several tissues including gastrointestinal tract, liver, testis, pancreas and several brain regions. Collectively, results from present study not only established a molecular basis to elucidate the physiological roles of SCT, SCT-LP and SCTR in chickens, but also provide critical insights into structural and functional changes of secretin and its receptor during vertebrate evolution. © 2011 Elsevier Ireland Ltd.en_HK
dc.languageengen_US
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/mceen_HK
dc.relation.ispartofMolecular and Cellular Endocrinologyen_HK
dc.subjectChickenen_HK
dc.subjectPancreasen_HK
dc.subjectSCTRen_HK
dc.subjectSecretinen_HK
dc.subjectSecretin-like peptideen_HK
dc.subject.meshAmino Acid Sequence-
dc.subject.meshCyclic AMP-Dependent Protein Kinases - metabolism-
dc.subject.meshReceptors, G-Protein-Coupled - genetics - metabolism-
dc.subject.meshReceptors, Gastrointestinal Hormone - genetics - metabolism-
dc.subject.meshSecretin - genetics - metabolism - pharmacology-
dc.titleCharacterization of chicken secretin (SCT) and secretin receptor (SCTR) genes: A novel secretin-like peptide (SCT-LP) and secretin encoded in a single geneen_HK
dc.typeArticleen_HK
dc.identifier.emailWang, Y: cdwyj@yahoo.comen_HK
dc.identifier.emailLeung, FC: fcleung@hkucc.hku.hken_HK
dc.identifier.authorityWang, Y=rp00801en_HK
dc.identifier.authorityLeung, FC=rp00731en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.mce.2011.09.012en_HK
dc.identifier.pmid21939730en_HK
dc.identifier.scopuseid_2-s2.0-80755132149en_HK
dc.identifier.hkuros198297en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80755132149&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume348en_HK
dc.identifier.issue1en_HK
dc.identifier.spage270en_HK
dc.identifier.epage280en_HK
dc.identifier.isiWOS:000297539400031-
dc.publisher.placeIrelanden_HK
dc.identifier.scopusauthoridWang, Y=36062525200en_HK
dc.identifier.scopusauthoridHuang, G=55069829200en_HK
dc.identifier.scopusauthoridLi, J=27172363500en_HK
dc.identifier.scopusauthoridMeng, F=54412646800en_HK
dc.identifier.scopusauthoridHe, X=54412327700en_HK
dc.identifier.scopusauthoridLeung, FC=7103078633en_HK
dc.identifier.citeulike9835125-

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