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Article: Characterization of chicken secretin (SCT) and secretin receptor (SCTR) genes: A novel secretin-like peptide (SCT-LP) and secretin encoded in a single gene
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TitleCharacterization of chicken secretin (SCT) and secretin receptor (SCTR) genes: A novel secretin-like peptide (SCT-LP) and secretin encoded in a single gene
 
AuthorsWang, Y2
Huang, G2
Li, J2
Meng, F2
He, X2
Leung, FC1
 
KeywordsChicken
Pancreas
SCTR
Secretin
Secretin-like peptide
 
Issue Date2012
 
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/mce
 
CitationMolecular And Cellular Endocrinology, 2012, v. 348 n. 1, p. 270-280 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.mce.2011.09.012
 
AbstractSecretin and the secretin receptor have been reported to play an important role in regulating pancreatic water and bicarbonate secretion in mammals; however, little is known about their expression, structure, and biological functions in non-mammalian vertebrates including birds. In this study, the full-length cDNAs encoding secretin and secretin receptor have first been cloned from duodenum of adult chickens. The putative chicken secretin receptor (cSCTR) is 449 amino acids in length and shares high sequence identity (58-63%) with its mammalian counterparts. Interestingly, chicken secretin cDNA encodes not only the secretin peptide (cSCT), but also a novel secretin-like peptide (cSCT-LP), which shares high amino acid identity with chicken (56%) and mammalian (48-52%) secretin. Using a pGL3-CRE-luciferase reporter system, we further demonstrated that both cSCT (EC 50: 0.31nM) and cSCT-LP (EC 50: 1.10nM), but not other structurally-related peptides, could potently activate cSCTR expressed in CHO cells, suggesting that both peptides may function as potential ligands for cSCTR. Using RT-PCR, the expression of secretin and secretin receptor in adult chicken tissues was also examined. Secretin was detected to be predominantly expressed in small intestine, while the mRNA expression of cSCTR was restricted to several tissues including gastrointestinal tract, liver, testis, pancreas and several brain regions. Collectively, results from present study not only established a molecular basis to elucidate the physiological roles of SCT, SCT-LP and SCTR in chickens, but also provide critical insights into structural and functional changes of secretin and its receptor during vertebrate evolution. © 2011 Elsevier Ireland Ltd.
 
ISSN0303-7207
2013 Impact Factor: 4.241
2013 SCImago Journal Rankings: 2.044
 
DOIhttp://dx.doi.org/10.1016/j.mce.2011.09.012
 
ISI Accession Number IDWOS:000297539400031
Funding AgencyGrant Number
National Natural Science Foundation of China30771171
30871338
30971569
Funding Information:

This work was supported by grants from the National Natural Science Foundation of China (30771171, 30871338, 30971569). Special thanks are given to Professor M.E. El-halwani (Department of Animal Science, University of Minnesota, Saint Paul, MN) for providing us with chicken VIP and turkey PHI. We would also like to give special thanks to the two anonymous reviewers for their constructive suggestions for the publication of this manuscript.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorWang, Y
 
dc.contributor.authorHuang, G
 
dc.contributor.authorLi, J
 
dc.contributor.authorMeng, F
 
dc.contributor.authorHe, X
 
dc.contributor.authorLeung, FC
 
dc.date.accessioned2012-02-03T06:20:10Z
 
dc.date.available2012-02-03T06:20:10Z
 
dc.date.issued2012
 
dc.description.abstractSecretin and the secretin receptor have been reported to play an important role in regulating pancreatic water and bicarbonate secretion in mammals; however, little is known about their expression, structure, and biological functions in non-mammalian vertebrates including birds. In this study, the full-length cDNAs encoding secretin and secretin receptor have first been cloned from duodenum of adult chickens. The putative chicken secretin receptor (cSCTR) is 449 amino acids in length and shares high sequence identity (58-63%) with its mammalian counterparts. Interestingly, chicken secretin cDNA encodes not only the secretin peptide (cSCT), but also a novel secretin-like peptide (cSCT-LP), which shares high amino acid identity with chicken (56%) and mammalian (48-52%) secretin. Using a pGL3-CRE-luciferase reporter system, we further demonstrated that both cSCT (EC 50: 0.31nM) and cSCT-LP (EC 50: 1.10nM), but not other structurally-related peptides, could potently activate cSCTR expressed in CHO cells, suggesting that both peptides may function as potential ligands for cSCTR. Using RT-PCR, the expression of secretin and secretin receptor in adult chicken tissues was also examined. Secretin was detected to be predominantly expressed in small intestine, while the mRNA expression of cSCTR was restricted to several tissues including gastrointestinal tract, liver, testis, pancreas and several brain regions. Collectively, results from present study not only established a molecular basis to elucidate the physiological roles of SCT, SCT-LP and SCTR in chickens, but also provide critical insights into structural and functional changes of secretin and its receptor during vertebrate evolution. © 2011 Elsevier Ireland Ltd.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationMolecular And Cellular Endocrinology, 2012, v. 348 n. 1, p. 270-280 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.mce.2011.09.012
 
dc.identifier.citeulike9835125
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.mce.2011.09.012
 
dc.identifier.epage280
 
dc.identifier.hkuros198297
 
dc.identifier.isiWOS:000297539400031
Funding AgencyGrant Number
National Natural Science Foundation of China30771171
30871338
30971569
Funding Information:

This work was supported by grants from the National Natural Science Foundation of China (30771171, 30871338, 30971569). Special thanks are given to Professor M.E. El-halwani (Department of Animal Science, University of Minnesota, Saint Paul, MN) for providing us with chicken VIP and turkey PHI. We would also like to give special thanks to the two anonymous reviewers for their constructive suggestions for the publication of this manuscript.

 
dc.identifier.issn0303-7207
2013 Impact Factor: 4.241
2013 SCImago Journal Rankings: 2.044
 
dc.identifier.issue1
 
dc.identifier.pmid21939730
 
dc.identifier.scopuseid_2-s2.0-80755132149
 
dc.identifier.spage270
 
dc.identifier.urihttp://hdl.handle.net/10722/144728
 
dc.identifier.volume348
 
dc.languageeng
 
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/mce
 
dc.publisher.placeIreland
 
dc.relation.ispartofMolecular and Cellular Endocrinology
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAmino Acid Sequence
 
dc.subject.meshCyclic AMP-Dependent Protein Kinases - metabolism
 
dc.subject.meshReceptors, G-Protein-Coupled - genetics - metabolism
 
dc.subject.meshReceptors, Gastrointestinal Hormone - genetics - metabolism
 
dc.subject.meshSecretin - genetics - metabolism - pharmacology
 
dc.subjectChicken
 
dc.subjectPancreas
 
dc.subjectSCTR
 
dc.subjectSecretin
 
dc.subjectSecretin-like peptide
 
dc.titleCharacterization of chicken secretin (SCT) and secretin receptor (SCTR) genes: A novel secretin-like peptide (SCT-LP) and secretin encoded in a single gene
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong
  2. Sichuan University