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Article: 2-D t-ITP/CZE determination of clinical urinary proteins using a microfluidic-chip capillary electrophoresis device

Title2-D t-ITP/CZE determination of clinical urinary proteins using a microfluidic-chip capillary electrophoresis device
Authors
Keywords2-D Electrophoresis
ITP
Microchip-CE
Protein
Urine
Issue Date2011
PublisherWiley - V C H Verlag GmbH & Co KGaA.
Citation
Electrophoresis, 2011, v. 32 n. 23, p. 3406-3414 How to Cite?
AbstractTo replace the time-consuming sample pretreatment procedure, a microfluidic chip-CE device incorporating on-chip sample desalting/preconcentration with transient isotachophoresis (ITP)/capillary zone electrophoresis (CZE) was fabricated to perform sequential on-chip sample pretreatment and CE determination of four urinary proteins in clinical samples. On-chip sample desalting, clean-up and analyte preconcentration enable removing interfering sample matrix prior to transferring analytes to separation capillary for transient ITP/CZE determination. Four important urinary proteins transferrin, β2-microglobulin, human serum albumin (HSA) and immunoglobulin G (IgG), investigated were shown to achieve quantitation limits sufficiently high to meet medical requirements, sensitivity enhancement up to 40-fold and detection limits down to 0.3, 0.05, 0.6, 0.5mg/L, respectively. Compared to the stacking effect, the use of a large sample size was found to be the major factor for sensitivity enhancement. The method reliability is established by close to 100% recoveries and statistical agreement of results from the method developed with currently used clinical radio-immunoassay method for all four proteins investigated. Moreover, an assay time of less than 10min is needed in the method developed as compared to 7h for the radio-immunoassay method. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Persistent Identifierhttp://hdl.handle.net/10722/144520
ISSN
2015 Impact Factor: 2.482
2015 SCImago Journal Rankings: 0.896
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWu, Ren_HK
dc.contributor.authorYeung, WSBen_HK
dc.contributor.authorFung, YSen_HK
dc.date.accessioned2012-02-03T06:12:04Z-
dc.date.available2012-02-03T06:12:04Z-
dc.date.issued2011en_HK
dc.identifier.citationElectrophoresis, 2011, v. 32 n. 23, p. 3406-3414en_HK
dc.identifier.issn0173-0835en_HK
dc.identifier.urihttp://hdl.handle.net/10722/144520-
dc.description.abstractTo replace the time-consuming sample pretreatment procedure, a microfluidic chip-CE device incorporating on-chip sample desalting/preconcentration with transient isotachophoresis (ITP)/capillary zone electrophoresis (CZE) was fabricated to perform sequential on-chip sample pretreatment and CE determination of four urinary proteins in clinical samples. On-chip sample desalting, clean-up and analyte preconcentration enable removing interfering sample matrix prior to transferring analytes to separation capillary for transient ITP/CZE determination. Four important urinary proteins transferrin, β2-microglobulin, human serum albumin (HSA) and immunoglobulin G (IgG), investigated were shown to achieve quantitation limits sufficiently high to meet medical requirements, sensitivity enhancement up to 40-fold and detection limits down to 0.3, 0.05, 0.6, 0.5mg/L, respectively. Compared to the stacking effect, the use of a large sample size was found to be the major factor for sensitivity enhancement. The method reliability is established by close to 100% recoveries and statistical agreement of results from the method developed with currently used clinical radio-immunoassay method for all four proteins investigated. Moreover, an assay time of less than 10min is needed in the method developed as compared to 7h for the radio-immunoassay method. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.en_HK
dc.languageengen_US
dc.publisherWiley - V C H Verlag GmbH & Co KGaA.-
dc.relation.ispartofElectrophoresisen_HK
dc.subject2-D Electrophoresisen_HK
dc.subjectITPen_HK
dc.subjectMicrochip-CEen_HK
dc.subjectProteinen_HK
dc.subjectUrineen_HK
dc.subject.meshElectrophoresis, Microchip - instrumentation - methods-
dc.subject.meshImmunoglobulin G - urine-
dc.subject.meshIsotachophoresis - instrumentation - methods-
dc.subject.meshProteins - analysis-
dc.subject.meshProteinuria - urine-
dc.title2-D t-ITP/CZE determination of clinical urinary proteins using a microfluidic-chip capillary electrophoresis deviceen_HK
dc.typeArticleen_HK
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.emailFung, YS:ysfung@hku.hken_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.identifier.authorityFung, YS=rp00697en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/elps.201100214en_HK
dc.identifier.pmid22134979-
dc.identifier.scopuseid_2-s2.0-82755162006en_HK
dc.identifier.hkuros198408en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-82755162006&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume32en_HK
dc.identifier.issue23en_HK
dc.identifier.spage3406en_HK
dc.identifier.epage3414en_HK
dc.identifier.eissn1522-2683-
dc.identifier.isiWOS:000298300900014-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridWu, R=54405220300en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK
dc.identifier.scopusauthoridFung, YS=13309754700en_HK

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