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Article: Microfluidic chip-capillary electrophoresis with dynamic multi-segment standard addition for rapidly identifying nephrolithiasis markers in urine

TitleMicrofluidic chip-capillary electrophoresis with dynamic multi-segment standard addition for rapidly identifying nephrolithiasis markers in urine
Authors
KeywordsBiomedical analysis
Kidney stone
Microchip CE
Organic acid
Urinary marker
Issue Date2011
PublisherWiley - V C H Verlag GmbH & Co KGaA.
Citation
Electrophoresis, 2011, v. 32 n. 23, p. 3437-3445 How to Cite?
AbstractA microchip-CE device was fabricated for bed-side monitoring of nephrolithiasis biomarkers in urine by incorporating on-chip continuous passive mixing and standard addition to reduce sample matrix interference, increase sample throughput and eliminate accessories for active mixing. Under optimized conditions with buffer containing 20mM borate and 0.5mM CTAB at pH 10.3, sample and standards injected electrokinetically at -350V for 10s for online mixing in a Y-merging flow microchannel prior to CE separation and UV detection at 210nm, both inhibitors (citrate, CA) and promoters (oxalate, OA and uric acid, UA) for nephrolithiasis can be separated and determined in human urine in a single run completed within 10min after a simple 50-fold sample dilution and filtering. Satisfactory working ranges from 0.13-40, 0.25-40 and 0.025-40mM, LOD 2.6, 6.1 and 0.7μM, repeatability (%RSD, n=5) for migration time 1.40, 1.43, 0.47 and peak area 4.46, 6.10, 1.98, respectively, for CA, OA and UA are obtained for urine samples. The use of on-chip standard addition is shown to improve repeatability of the migration time, assist the identification of nephrolithiasis markers from difficult samples with noisy baseline and enlarge the working range for nephrolithiasis marker determination. The device developed can be used for both routine and emergency monitoring to deliver results on demand for bedside monitoring and public health protection. It provides an early detection of nephrolithiasis to enable timely treatments, ease anxiety of parents for neonates consuming suspected contaminated food, and quick results for patients in a critical condition. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Persistent Identifierhttp://hdl.handle.net/10722/144519
ISSN
2015 Impact Factor: 2.482
2015 SCImago Journal Rankings: 0.896
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Research Grants CouncilRGC 701508P
Hong Kong University Research CommitteeHKU 200711159151
HKU 200902160037
Funding Information:

The authors acknowledge the financial support from the Hong Kong Research Grants Council (RGC 701508P) and Seed Funding from the Hong Kong University Research Committee (HKU 200711159151 and HKU 200902160037).

References

 

DC FieldValueLanguage
dc.contributor.authorGuo, WPen_HK
dc.contributor.authorFung, YSen_HK
dc.date.accessioned2012-02-03T06:12:03Z-
dc.date.available2012-02-03T06:12:03Z-
dc.date.issued2011en_HK
dc.identifier.citationElectrophoresis, 2011, v. 32 n. 23, p. 3437-3445en_HK
dc.identifier.issn0173-0835en_HK
dc.identifier.urihttp://hdl.handle.net/10722/144519-
dc.description.abstractA microchip-CE device was fabricated for bed-side monitoring of nephrolithiasis biomarkers in urine by incorporating on-chip continuous passive mixing and standard addition to reduce sample matrix interference, increase sample throughput and eliminate accessories for active mixing. Under optimized conditions with buffer containing 20mM borate and 0.5mM CTAB at pH 10.3, sample and standards injected electrokinetically at -350V for 10s for online mixing in a Y-merging flow microchannel prior to CE separation and UV detection at 210nm, both inhibitors (citrate, CA) and promoters (oxalate, OA and uric acid, UA) for nephrolithiasis can be separated and determined in human urine in a single run completed within 10min after a simple 50-fold sample dilution and filtering. Satisfactory working ranges from 0.13-40, 0.25-40 and 0.025-40mM, LOD 2.6, 6.1 and 0.7μM, repeatability (%RSD, n=5) for migration time 1.40, 1.43, 0.47 and peak area 4.46, 6.10, 1.98, respectively, for CA, OA and UA are obtained for urine samples. The use of on-chip standard addition is shown to improve repeatability of the migration time, assist the identification of nephrolithiasis markers from difficult samples with noisy baseline and enlarge the working range for nephrolithiasis marker determination. The device developed can be used for both routine and emergency monitoring to deliver results on demand for bedside monitoring and public health protection. It provides an early detection of nephrolithiasis to enable timely treatments, ease anxiety of parents for neonates consuming suspected contaminated food, and quick results for patients in a critical condition. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.en_HK
dc.languageengen_US
dc.publisherWiley - V C H Verlag GmbH & Co KGaA.-
dc.relation.ispartofElectrophoresisen_HK
dc.subjectBiomedical analysisen_HK
dc.subjectKidney stoneen_HK
dc.subjectMicrochip CEen_HK
dc.subjectOrganic aciden_HK
dc.subjectUrinary markeren_HK
dc.subject.meshBiological Markers - urine-
dc.subject.meshCitric Acid - urine-
dc.subject.meshElectrophoresis, Microchip - instrumentation - methods-
dc.subject.meshEquipment Design-
dc.subject.meshNephrolithiasis - diagnosis - urine-
dc.titleMicrofluidic chip-capillary electrophoresis with dynamic multi-segment standard addition for rapidly identifying nephrolithiasis markers in urineen_HK
dc.typeArticleen_HK
dc.identifier.emailFung, YS:ysfung@hku.hken_HK
dc.identifier.authorityFung, YS=rp00697en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/elps.201100213en_HK
dc.identifier.pmid22134981-
dc.identifier.scopuseid_2-s2.0-82755189084en_HK
dc.identifier.hkuros198407en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-82755189084&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume32en_HK
dc.identifier.issue23en_HK
dc.identifier.spage3437en_HK
dc.identifier.epage3445en_HK
dc.identifier.eissn1522-2683-
dc.identifier.isiWOS:000298300900018-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridGuo, WP=37025980200en_HK
dc.identifier.scopusauthoridFung, YS=13309754700en_HK

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