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Article: TRAPPC9 mediates the interaction between p150 glued and COPII vesicles at the target membrane

TitleTRAPPC9 mediates the interaction between p150 glued and COPII vesicles at the target membrane
Authors
KeywordsProtein p150 glued
Trappc9 protein
Carboxy terminal sequence
Endoplasmic reticulum
Microtubule
Issue Date2012
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
Citation
Plos One, 2012, v. 7 n. 1 How to Cite?
AbstractBackground: The transport of endoplasmic reticulum (ER)-derived COPII vesicles toward the ER-Golgi intermediate compartment (ERGIC) requires cytoplasmic dynein and is dependent on microtubules. p150 Glued, a subunit of dynactin, has been implicated in the transport of COPII vesicles via its interaction with COPII coat components Sec23 and Sec24. However, whether and how COPII vesicle tether, TRAPP (Transport protein particle), plays a role in the interaction between COPII vesicles and microtubules is currently unknown. Principle Findings: We address the functional relationship between COPII tether TRAPP and dynactin. Overexpressed TRAPP subunits interfered with microtubule architecture by competing p150 Glued away from the MTOC. TRAPP subunit TRAPPC9 bound directly to p150 Glued via the same carboxyl terminal domain of p150 Glued that binds Sec23 and Sec24. TRAPPC9 also inhibited the interaction between p150 Glued and Sec23/Sec24 both in vitro and in vivo, suggesting that TRAPPC9 serves to uncouple p150 Glued from the COPII coat, and to relay the vesicle-dynactin interaction at the target membrane. Conclusions: These findings provide a new perspective on the function of TRAPP as an adaptor between the ERGIC membrane and dynactin. By preserving the connection between dynactin and the tethered and/or fused vesicles, TRAPP allows nascent ERGIC to continue the movement along the microtubules as they mature into the cis-Golgi. © 2012 Zong et al.
Persistent Identifierhttp://hdl.handle.net/10722/144516
ISSN
2015 Impact Factor: 3.057
2015 SCImago Journal Rankings: 1.395
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Research Grant Council479410
Funding Information:

This work is supported by the general research fund to SY (grant number 479410) by the Hong Kong Research Grant Council. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

References

 

DC FieldValueLanguage
dc.contributor.authorZong, Men_HK
dc.contributor.authorSatoh, Aen_HK
dc.contributor.authorYu, MKen_HK
dc.contributor.authorSiu, KYen_HK
dc.contributor.authorNg, WYen_HK
dc.contributor.authorChan, HCen_HK
dc.contributor.authorTanner, JAen_HK
dc.contributor.authorYu, Sen_HK
dc.date.accessioned2012-02-03T06:11:54Z-
dc.date.available2012-02-03T06:11:54Z-
dc.date.issued2012en_HK
dc.identifier.citationPlos One, 2012, v. 7 n. 1en_HK
dc.identifier.issn1932-6203en_HK
dc.identifier.urihttp://hdl.handle.net/10722/144516-
dc.description.abstractBackground: The transport of endoplasmic reticulum (ER)-derived COPII vesicles toward the ER-Golgi intermediate compartment (ERGIC) requires cytoplasmic dynein and is dependent on microtubules. p150 Glued, a subunit of dynactin, has been implicated in the transport of COPII vesicles via its interaction with COPII coat components Sec23 and Sec24. However, whether and how COPII vesicle tether, TRAPP (Transport protein particle), plays a role in the interaction between COPII vesicles and microtubules is currently unknown. Principle Findings: We address the functional relationship between COPII tether TRAPP and dynactin. Overexpressed TRAPP subunits interfered with microtubule architecture by competing p150 Glued away from the MTOC. TRAPP subunit TRAPPC9 bound directly to p150 Glued via the same carboxyl terminal domain of p150 Glued that binds Sec23 and Sec24. TRAPPC9 also inhibited the interaction between p150 Glued and Sec23/Sec24 both in vitro and in vivo, suggesting that TRAPPC9 serves to uncouple p150 Glued from the COPII coat, and to relay the vesicle-dynactin interaction at the target membrane. Conclusions: These findings provide a new perspective on the function of TRAPP as an adaptor between the ERGIC membrane and dynactin. By preserving the connection between dynactin and the tethered and/or fused vesicles, TRAPP allows nascent ERGIC to continue the movement along the microtubules as they mature into the cis-Golgi. © 2012 Zong et al.en_HK
dc.languageengen_US
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.actionen_HK
dc.relation.ispartofPLoS ONEen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectProtein p150 glued-
dc.subjectTrappc9 protein-
dc.subjectCarboxy terminal sequence-
dc.subjectEndoplasmic reticulum-
dc.subjectMicrotubule-
dc.titleTRAPPC9 mediates the interaction between p150 glued and COPII vesicles at the target membraneen_HK
dc.typeArticleen_HK
dc.identifier.emailTanner, JA:jatanner@hku.hken_HK
dc.identifier.authorityTanner, JA=rp00495en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1371/journal.pone.0029995en_HK
dc.identifier.pmid22279557-
dc.identifier.pmcidPMC3261171-
dc.identifier.scopuseid_2-s2.0-84855964001en_HK
dc.identifier.hkuros198568en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84855964001&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume7en_HK
dc.identifier.issue1en_HK
dc.identifier.isiWOS:000299771900035-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridZong, M=37119693000en_HK
dc.identifier.scopusauthoridSatoh, A=7103340616en_HK
dc.identifier.scopusauthoridYu, MK=54897827500en_HK
dc.identifier.scopusauthoridSiu, KY=54897554100en_HK
dc.identifier.scopusauthoridNg, WY=54897767500en_HK
dc.identifier.scopusauthoridChan, HC=54898798800en_HK
dc.identifier.scopusauthoridTanner, JA=35513993000en_HK
dc.identifier.scopusauthoridYu, S=54898395300en_HK

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