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Article: Mequindox induced cellular DNA damage via generation of reactive oxygen species
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TitleMequindox induced cellular DNA damage via generation of reactive oxygen species
 
AuthorsLiu, J2
Ouyang, M2
Jiang, J2
Mu, P2
Wu, J2
Yang, Q2
Zhang, C2
Xu, W2
Wang, L2
Huen, MSY1
Deng, Y2
 
Issue Date2012
 
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/gentox
 
CitationMutation Research - Genetic Toxicology And Environmental Mutagenesis, 2012, v. 741 n. 1-2, p. 70-75 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.mrgentox.2011.10.012
 
AbstractMequindox, a quinoxaline-N-dioxide derivative that possesses antibacterial properties, has been widely used as a feed additive in the stockbreeding industry in China. While recent pharmacological studies have uncovered potential hazardous effects of mequindox, exactly how mequindox induces pathological changes and the cellular responses associated with its consumption remain largely unexplored. In this study, we investigated the cellular responses associated with mequindox treatment. We report here that mequindox inhibits cell proliferation by arresting cells at the G2/M phase of the cell cycle. Interestingly, this mequindox-associated deleterious effect on cell proliferation was observed in human, pig as well as chicken cells, suggesting that mequindox acts on evolutionarily conserved target(s). To further understand the mequindox-host interaction and the mechanism underlying mequindox-induced cell cycle arrest, we measured the cellular content of DNA damage, which is known to perturb cell proliferation and compromise cell survival. Accordingly, using γ-H2AX as a surrogate marker for DNA damage, we found that mequindox treatment induced cellular DNA damage, which paralleled the chemical-induced elevation of reactive oxygen species (ROS) levels. Importantly, expression of the antioxidant enzyme catalase partially alleviated these mequindox-associated effects. Taken together, our results suggest that mequindox cytotoxicity is attributable, in part, to its role as a potent inducer of DNA damage via ROS. © 2011 Elsevier B.V.
 
ISSN1383-5718
2013 Impact Factor: 2.481
2013 SCImago Journal Rankings: 0.833
 
DOIhttp://dx.doi.org/10.1016/j.mrgentox.2011.10.012
 
ISI Accession Number IDWOS:000300746800008
Funding AgencyGrant Number
National Basic Research Program of China (973 Program)2009CB118802
Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme
Program for New Century Excellent Talents in UniversityNCET-08-0643
Guangdong Province Universities and Colleges Special Funds for Talents
Funding Information:

This work was supported by the National Basic Research Program of China (973 Program), no: 2009CB118802; Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme (2009); The Program for New Century Excellent Talents in University, no: NCET-08-0643; Guangdong Province Universities and Colleges Special Funds for Talents (2010).

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorLiu, J
 
dc.contributor.authorOuyang, M
 
dc.contributor.authorJiang, J
 
dc.contributor.authorMu, P
 
dc.contributor.authorWu, J
 
dc.contributor.authorYang, Q
 
dc.contributor.authorZhang, C
 
dc.contributor.authorXu, W
 
dc.contributor.authorWang, L
 
dc.contributor.authorHuen, MSY
 
dc.contributor.authorDeng, Y
 
dc.date.accessioned2012-02-03T06:11:39Z
 
dc.date.available2012-02-03T06:11:39Z
 
dc.date.issued2012
 
dc.description.abstractMequindox, a quinoxaline-N-dioxide derivative that possesses antibacterial properties, has been widely used as a feed additive in the stockbreeding industry in China. While recent pharmacological studies have uncovered potential hazardous effects of mequindox, exactly how mequindox induces pathological changes and the cellular responses associated with its consumption remain largely unexplored. In this study, we investigated the cellular responses associated with mequindox treatment. We report here that mequindox inhibits cell proliferation by arresting cells at the G2/M phase of the cell cycle. Interestingly, this mequindox-associated deleterious effect on cell proliferation was observed in human, pig as well as chicken cells, suggesting that mequindox acts on evolutionarily conserved target(s). To further understand the mequindox-host interaction and the mechanism underlying mequindox-induced cell cycle arrest, we measured the cellular content of DNA damage, which is known to perturb cell proliferation and compromise cell survival. Accordingly, using γ-H2AX as a surrogate marker for DNA damage, we found that mequindox treatment induced cellular DNA damage, which paralleled the chemical-induced elevation of reactive oxygen species (ROS) levels. Importantly, expression of the antioxidant enzyme catalase partially alleviated these mequindox-associated effects. Taken together, our results suggest that mequindox cytotoxicity is attributable, in part, to its role as a potent inducer of DNA damage via ROS. © 2011 Elsevier B.V.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationMutation Research - Genetic Toxicology And Environmental Mutagenesis, 2012, v. 741 n. 1-2, p. 70-75 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.mrgentox.2011.10.012
 
dc.identifier.citeulike10014603
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.mrgentox.2011.10.012
 
dc.identifier.epage75
 
dc.identifier.hkuros198264
 
dc.identifier.isiWOS:000300746800008
Funding AgencyGrant Number
National Basic Research Program of China (973 Program)2009CB118802
Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme
Program for New Century Excellent Talents in UniversityNCET-08-0643
Guangdong Province Universities and Colleges Special Funds for Talents
Funding Information:

This work was supported by the National Basic Research Program of China (973 Program), no: 2009CB118802; Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme (2009); The Program for New Century Excellent Talents in University, no: NCET-08-0643; Guangdong Province Universities and Colleges Special Funds for Talents (2010).

 
dc.identifier.issn1383-5718
2013 Impact Factor: 2.481
2013 SCImago Journal Rankings: 0.833
 
dc.identifier.issue1-2
 
dc.identifier.pmid22094289
 
dc.identifier.scopuseid_2-s2.0-83955161129
 
dc.identifier.spage70
 
dc.identifier.urihttp://hdl.handle.net/10722/144508
 
dc.identifier.volume741
 
dc.languageeng
 
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/gentox
 
dc.publisher.placeNetherlands
 
dc.relation.ispartofMutation Research - Genetic Toxicology and Environmental Mutagenesis
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAnimal Husbandry
 
dc.subject.meshAnti-Bacterial Agents - toxicity
 
dc.subject.meshCell Line, Tumor
 
dc.subject.meshCell Survival - drug effects
 
dc.subject.meshDNA Damage
 
dc.subject.meshHumans
 
dc.subject.meshMutagens - toxicity
 
dc.subject.meshQuinoxalines - toxicity
 
dc.subject.meshReactive Oxygen Species - metabolism
 
dc.titleMequindox induced cellular DNA damage via generation of reactive oxygen species
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong
  2. South China Agricultural University