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Article: The effect of quercetin on expression of SOX9 and subsequent release of type II collagen in spheno-occipital synchondroses of organ-cultured mice

TitleThe effect of quercetin on expression of SOX9 and subsequent release of type II collagen in spheno-occipital synchondroses of organ-cultured mice
Authors
Apichart, VWong, RRabie, BLei, S
KeywordsQuercetin
Synchondrosis
Issue Date2012
PublisherE H Angle Education and Research Foundation, Inc. The Journal's web site is located at http://www.angle.org/
Citation
Angle Orthodontist, 2012, v. 82 n. 2, p. 247-253 How to Cite?
DOI: http://dx.doi.org/10.2319/042111-278.1
AbstractObjective: To identify the expressions of SOX9 and type II collagen in spheno-occipital synchondrosis in response to quercetin, using a mouse in vitro model. Materials and Methods: A total of 50 one-day-old male BALB/c mice were randomly assigned to the control and experimental groups. Each group was subdivided into five different time points, which were 6, 24, 48, 72, and 168 hours, and each subgroup contained 5 mice (n = 5). In the experimental group, the spheno-occipital synchondrosis was immersed in the BGJb medium + quercetin dihydrate 1 μM. In the control group, the spheno-occipital synchondrosis was immersed in the BGJb medium. Tissue sections were subjected to immunohistochemical staining for SOX9 and type II collagen expressions. Results: Quantitative analysis revealed there was a statistically significant increase of 32.31% (P <.001) in the expression of SOX9 between experimental groups and control groups at 24 hours. Furthermore, there was a statistically significant increase of 22.99% (P <.001) in the expression of type II collagen between experimental groups and control groups at 72 hours. Conclusion: The expressions of SOX9 and type II collagen in the spheno-occipital synchondrosis can be increased by quercetin. © 2012 by The EH Angle Education and Research Foundation, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/144500
ISSN
0003-3219
2021 Impact Factor: 2.684
2020 SCImago Journal Rankings: 1.116
ISI Accession Number ID
WOS:000301393200010
Funding AgencyGrant Number
Committee on Research and Conference Grants, the University of Hong KongCRCG 10207932
36170.08003.323.01
Funding Information:

This research was supported by the Committee on Research and Conference Grants, the University of Hong Kong (CRCG 10207932, 36170.08003.323.01). We thank Dr Shuxia Li and Mr Chui Ying Yip of the Hard Tissue Laboratory for their technical assistance in the histological preparation and also Mr Shadow Yeung for his kind assistance with the statistical analysis.

References
References in Scopus

 

DC FieldValueLanguage
dc.contributor.authorApichart, Ven_HK
dc.contributor.authorWong, Ren_HK
dc.contributor.authorRabie, Ben_HK
dc.contributor.authorLei, Sen_HK
dc.date.accessioned2012-02-03T06:11:27Z-
dc.date.available2012-02-03T06:11:27Z-
dc.date.issued2012en_HK
dc.identifier.citationAngle Orthodontist, 2012, v. 82 n. 2, p. 247-253en_HK
dc.identifier.issn0003-3219en_HK
dc.identifier.urihttp://hdl.handle.net/10722/144500-
dc.description.abstractObjective: To identify the expressions of SOX9 and type II collagen in spheno-occipital synchondrosis in response to quercetin, using a mouse in vitro model. Materials and Methods: A total of 50 one-day-old male BALB/c mice were randomly assigned to the control and experimental groups. Each group was subdivided into five different time points, which were 6, 24, 48, 72, and 168 hours, and each subgroup contained 5 mice (n = 5). In the experimental group, the spheno-occipital synchondrosis was immersed in the BGJb medium + quercetin dihydrate 1 μM. In the control group, the spheno-occipital synchondrosis was immersed in the BGJb medium. Tissue sections were subjected to immunohistochemical staining for SOX9 and type II collagen expressions. Results: Quantitative analysis revealed there was a statistically significant increase of 32.31% (P <.001) in the expression of SOX9 between experimental groups and control groups at 24 hours. Furthermore, there was a statistically significant increase of 22.99% (P <.001) in the expression of type II collagen between experimental groups and control groups at 72 hours. Conclusion: The expressions of SOX9 and type II collagen in the spheno-occipital synchondrosis can be increased by quercetin. © 2012 by The EH Angle Education and Research Foundation, Inc.en_HK
dc.languageengen_US
dc.publisherE H Angle Education and Research Foundation, Inc. The Journal's web site is located at http://www.angle.org/en_HK
dc.relation.ispartofAngle Orthodontisten_HK
dc.subjectQuercetinen_HK
dc.subjectSynchondrosisen_HK
dc.titleThe effect of quercetin on expression of SOX9 and subsequent release of type II collagen in spheno-occipital synchondroses of organ-cultured miceen_HK
dc.typeArticleen_HK
dc.identifier.emailWong, R:fyoung@hkucc.hku.hken_HK
dc.identifier.authorityWong, R=rp00038en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.2319/042111-278.1en_HK
dc.identifier.pmid21932938-
dc.identifier.scopuseid_2-s2.0-84858629895en_HK
dc.identifier.hkuros198296en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84858629895&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume82en_HK
dc.identifier.issue2en_HK
dc.identifier.spage247en_HK
dc.identifier.epage253en_HK
dc.identifier.isiWOS:000301393200010-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridApichart, V=55115716500en_HK
dc.identifier.scopusauthoridWong, R=7402127170en_HK
dc.identifier.scopusauthoridRabie, B=55117787800en_HK
dc.identifier.scopusauthoridLei, S=55113240500en_HK
dc.identifier.issnl0003-3219-

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