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Article: Growth characteristics of Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 as affected by different neutralisers

TitleGrowth characteristics of Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 as affected by different neutralisers
Authors
KeywordsAmmonium Compounds
Cells
Dairy Products
Enzyme Kinetics
Fermentation
Glucose
Growth Kinetics
Hydrolysis
Parameter Estimation
Ph Effects
Polysaccharides
Issue Date2005
Citation
Australian Journal Of Dairy Technology, 2005, v. 60 n. 1, p. 3-9 How to Cite?
AbstractSodium, potassium or ammonium hydroxide were used to control pH during the cultivation of Lactabacillus delbrueckii subsp. bulgaricus ATCC 11842 (Lb11842) in skim milk at a constant pH of 5.6 at 43°C for 10 h for production of the crude cellular extract as a source of β-D-galactosidase. Cell growth, lactic acid production, β-D-galactosidase and proteolytic activities were monitored. The addition of NaOH or KOH resulted in slight but insignificant (p>0.05) differences on all parameters, while all parameters were significantly (p<0.05) affected by the addition of NH4OH. The proteolytic activity of Lb11842 was significantly lower (p<0.05) in the presence of ammonium hydroxide. The exopolysaccharides (EPS) production was also significantly higher (p<0.05) in the presence of NH4OH. The higher EPS content significantly (p<0.05) affected the apparent viscosity of the concentrated, cell-free fermentation medium resulting in pseudo-plastic behavior.
Persistent Identifierhttp://hdl.handle.net/10722/144454
ISSN
2012 Impact Factor: 0.415
2013 SCImago Journal Rankings: 0.203
References

 

DC FieldValueLanguage
dc.contributor.authorVasiljevic, Ten_HK
dc.contributor.authorShah, NPen_HK
dc.contributor.authorJelen, Pen_HK
dc.date.accessioned2012-01-20T09:02:09Z-
dc.date.available2012-01-20T09:02:09Z-
dc.date.issued2005en_HK
dc.identifier.citationAustralian Journal Of Dairy Technology, 2005, v. 60 n. 1, p. 3-9en_HK
dc.identifier.issn0004-9433en_HK
dc.identifier.urihttp://hdl.handle.net/10722/144454-
dc.description.abstractSodium, potassium or ammonium hydroxide were used to control pH during the cultivation of Lactabacillus delbrueckii subsp. bulgaricus ATCC 11842 (Lb11842) in skim milk at a constant pH of 5.6 at 43°C for 10 h for production of the crude cellular extract as a source of β-D-galactosidase. Cell growth, lactic acid production, β-D-galactosidase and proteolytic activities were monitored. The addition of NaOH or KOH resulted in slight but insignificant (p>0.05) differences on all parameters, while all parameters were significantly (p<0.05) affected by the addition of NH4OH. The proteolytic activity of Lb11842 was significantly lower (p<0.05) in the presence of ammonium hydroxide. The exopolysaccharides (EPS) production was also significantly higher (p<0.05) in the presence of NH4OH. The higher EPS content significantly (p<0.05) affected the apparent viscosity of the concentrated, cell-free fermentation medium resulting in pseudo-plastic behavior.en_HK
dc.languageengen_US
dc.relation.ispartofAustralian Journal of Dairy Technologyen_HK
dc.subjectAmmonium Compoundsen_US
dc.subjectCellsen_US
dc.subjectDairy Productsen_US
dc.subjectEnzyme Kineticsen_US
dc.subjectFermentationen_US
dc.subjectGlucoseen_US
dc.subjectGrowth Kineticsen_US
dc.subjectHydrolysisen_US
dc.subjectParameter Estimationen_US
dc.subjectPh Effectsen_US
dc.subjectPolysaccharidesen_US
dc.titleGrowth characteristics of Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 as affected by different neutralisersen_HK
dc.typeArticleen_HK
dc.identifier.emailShah, NP: npshah@hku.hken_HK
dc.identifier.authorityShah, NP=rp01571en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-18444395845en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-18444395845&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume60en_HK
dc.identifier.issue1en_HK
dc.identifier.spage3en_HK
dc.identifier.epage9en_HK
dc.identifier.scopusauthoridVasiljevic, T=8576782100en_HK
dc.identifier.scopusauthoridShah, NP=7401823907en_HK
dc.identifier.scopusauthoridJelen, P=7004283846en_HK

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