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Article: A comparative study of bone marrow and peripheral blood CD34 + myeloblasts in acute myeloid leukaemia

TitleA comparative study of bone marrow and peripheral blood CD34 + myeloblasts in acute myeloid leukaemia
Authors
Issue Date2009
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/BJH
Citation
British Journal Of Haematology, 2009, v. 144 n. 4, p. 484-491 How to Cite?
AbstractTo examine the differences between primitive bone marrow (BM) and peripheral blood (PB) myeloblasts in acute myeloid leukaemia (AML), we compared CD34 + myeloblasts of paired BM and PB samples from 14 AML patients in terms of surface phenotype, homing and engraftment in a xenogeneic transplantation model, and gene expression, based on microarray studies and quantitative polymerase chain reaction. While there was no significant difference in surface phenotypes between these two populations, in vivo assay showed significantly better homing potential of PB CD34 + cells than BM CD34 + cells. Significant correlation between homing and engraftment in AML samples was also noted. In addition, gene expression profiling of CD34 + cells from five paired BM and PB leukaemic samples showed that genes involved in G-protein and prostaglandin signalling, chemotaxis and stress response, cell proliferation and apoptosis were down-regulated in PB CD34 + myeloblasts. These data suggested that circulating primitive myeloblasts in AML are functionally different from those residing in the marrow compartment, and such differences may be partly regulated by the BM microenvironment. © 2008 The Authors.
Persistent Identifierhttp://hdl.handle.net/10722/144287
ISSN
2015 Impact Factor: 5.401
2015 SCImago Journal Rankings: 2.313
ISI Accession Number ID
Funding AgencyGrant Number
University of Hong Kong
Funding Information:

We thank Dr. Agnes SW Chan, Microarray Bioinformatics Specialist of the Genome Centre, University of Hong Kong, for her helpful comments on the data analysis and Prof. Y.L. Kwong, Department of Medicine, University of Hong Kong, for his helpful comments and discussions on the manuscript. We also thank Ms Jenny Chan and Mr. Tsui CO in Department of Medicine, Queen Mary Hospital, for their assistance in sample processing. This work was supported by the University of Hong Kong.

References

 

DC FieldValueLanguage
dc.contributor.authorCheung, AMSen_HK
dc.contributor.authorChow, HCHen_HK
dc.contributor.authorLiang, Ren_HK
dc.contributor.authorLeung, AYHen_HK
dc.date.accessioned2012-01-20T08:59:47Z-
dc.date.available2012-01-20T08:59:47Z-
dc.date.issued2009en_HK
dc.identifier.citationBritish Journal Of Haematology, 2009, v. 144 n. 4, p. 484-491en_HK
dc.identifier.issn0007-1048en_HK
dc.identifier.urihttp://hdl.handle.net/10722/144287-
dc.description.abstractTo examine the differences between primitive bone marrow (BM) and peripheral blood (PB) myeloblasts in acute myeloid leukaemia (AML), we compared CD34 + myeloblasts of paired BM and PB samples from 14 AML patients in terms of surface phenotype, homing and engraftment in a xenogeneic transplantation model, and gene expression, based on microarray studies and quantitative polymerase chain reaction. While there was no significant difference in surface phenotypes between these two populations, in vivo assay showed significantly better homing potential of PB CD34 + cells than BM CD34 + cells. Significant correlation between homing and engraftment in AML samples was also noted. In addition, gene expression profiling of CD34 + cells from five paired BM and PB leukaemic samples showed that genes involved in G-protein and prostaglandin signalling, chemotaxis and stress response, cell proliferation and apoptosis were down-regulated in PB CD34 + myeloblasts. These data suggested that circulating primitive myeloblasts in AML are functionally different from those residing in the marrow compartment, and such differences may be partly regulated by the BM microenvironment. © 2008 The Authors.en_HK
dc.languageengen_US
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/BJHen_HK
dc.relation.ispartofBritish Journal of Haematologyen_HK
dc.subject.meshAdulten_HK
dc.subject.meshAgeden_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAntigens, CD34 - analysisen_HK
dc.subject.meshAntigens, Neoplasm - analysisen_HK
dc.subject.meshBone Marrow Cells - physiologyen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGene Expression Profiling - methodsen_HK
dc.subject.meshGraft Survivalen_HK
dc.subject.meshGranulocyte Precursor Cells - physiologyen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImmunophenotypingen_HK
dc.subject.meshLeukemia, Myeloid, Acute - blood - pathologyen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, SCIDen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshNeoplasm Transplantationen_HK
dc.subject.meshProspective Studiesen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction - methodsen_HK
dc.subject.meshTransplantation, Heterologousen_HK
dc.titleA comparative study of bone marrow and peripheral blood CD34 + myeloblasts in acute myeloid leukaemiaen_HK
dc.typeArticleen_HK
dc.identifier.emailCheung, AMS:h9945256@graduate.hku.hken_HK
dc.identifier.emailLiang, R:rliang@hku.hken_HK
dc.identifier.emailLeung, AYH:ayhleung@hku.hken_HK
dc.identifier.authorityCheung, AMS=rp01572en_HK
dc.identifier.authorityLiang, R=rp00345en_HK
dc.identifier.authorityLeung, AYH=rp00265en_HK
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1111/j.1365-2141.2008.07431.xen_HK
dc.identifier.pmid19055666-
dc.identifier.scopuseid_2-s2.0-58549088488en_HK
dc.identifier.hkuros157063-
dc.identifier.hkuros200892-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-58549088488&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume144en_HK
dc.identifier.issue4en_HK
dc.identifier.spage484en_HK
dc.identifier.epage491en_HK
dc.identifier.eissn1365-2141-
dc.identifier.isiWOS:000262635500003-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridCheung, AMS=36985759800en_HK
dc.identifier.scopusauthoridChow, HCH=7102303391en_HK
dc.identifier.scopusauthoridLiang, R=26643224900en_HK
dc.identifier.scopusauthoridLeung, AYH=7403012668en_HK
dc.identifier.citeulike3939570-

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