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Article: Direct AAV-mediated gene delivery to the temporomandibular joint

TitleDirect AAV-mediated gene delivery to the temporomandibular joint
Authors
KeywordsAdeno-associated virus
Enhanced green fluorescence protein
Gene therapy
Mandibular condyle
PCR
Issue Date2007
PublisherFrontiers in Bioscience. The Journal's web site is located at http://www.frontbiosci.org/
Citation
Frontiers In Bioscience, 2007, v. 12 n. 6, p. 2212-2220 How to Cite?
AbstractSuccessful intra-temporomandibular joint gene transfer is a prerequisite for gene therapy to induce mandibular condylar growth. This study was carried out to investigate the expression of recombinant adeno-associated virus (rAAV) based vector-mediated enhanced green fluorescence protein (eGFP) in the mandibular condyle. The transduction efficiencies for primary chondrocytes using different hybrids of rAAV vectors were identified by fluorescence microscopy and FACS. Sprague-Dawley rats were injected with either rAAV 2/2-eGFP construct or saline into both mandibular condyles. The spatial and temporal transgene expression was detected by in situ hybridization, RT-PCR and real-time PCR. Our result showed that rAAVs were capable of infecting rat chondrocytes. rAAV 2/2 was the best serotype to infect chondrocytes in vitro. By in situ hybridization, eGFP expression was clearly detected in the deeper layer of mandibular condyle as early as 7 days after injection. By real-time PCR, the transgene expression reached a peak at 21 days. This study was the first report to explore that rAAV-mediated genes could be transferred to mandibular condyle in vivo. Our results strongly suggest that rAAV 2/2 mediated gene delivery is a promising approach to deliver candidate genes for future regulating mandibular condylar growth.
Persistent Identifierhttp://hdl.handle.net/10722/144274
ISSN
2015 Impact Factor: 2.484
2015 SCImago Journal Rankings: 1.583
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorDai, Jen_HK
dc.contributor.authorRabie, ABMen_HK
dc.date.accessioned2012-01-20T08:58:42Z-
dc.date.available2012-01-20T08:58:42Z-
dc.date.issued2007en_HK
dc.identifier.citationFrontiers In Bioscience, 2007, v. 12 n. 6, p. 2212-2220en_HK
dc.identifier.issn1093-9946en_HK
dc.identifier.urihttp://hdl.handle.net/10722/144274-
dc.description.abstractSuccessful intra-temporomandibular joint gene transfer is a prerequisite for gene therapy to induce mandibular condylar growth. This study was carried out to investigate the expression of recombinant adeno-associated virus (rAAV) based vector-mediated enhanced green fluorescence protein (eGFP) in the mandibular condyle. The transduction efficiencies for primary chondrocytes using different hybrids of rAAV vectors were identified by fluorescence microscopy and FACS. Sprague-Dawley rats were injected with either rAAV 2/2-eGFP construct or saline into both mandibular condyles. The spatial and temporal transgene expression was detected by in situ hybridization, RT-PCR and real-time PCR. Our result showed that rAAVs were capable of infecting rat chondrocytes. rAAV 2/2 was the best serotype to infect chondrocytes in vitro. By in situ hybridization, eGFP expression was clearly detected in the deeper layer of mandibular condyle as early as 7 days after injection. By real-time PCR, the transgene expression reached a peak at 21 days. This study was the first report to explore that rAAV-mediated genes could be transferred to mandibular condyle in vivo. Our results strongly suggest that rAAV 2/2 mediated gene delivery is a promising approach to deliver candidate genes for future regulating mandibular condylar growth.en_HK
dc.languageengen_US
dc.publisherFrontiers in Bioscience. The Journal's web site is located at http://www.frontbiosci.org/en_HK
dc.relation.ispartofFrontiers in Bioscienceen_HK
dc.subjectAdeno-associated virusen_HK
dc.subjectEnhanced green fluorescence proteinen_HK
dc.subjectGene therapyen_HK
dc.subjectMandibular condyleen_HK
dc.subjectPCRen_HK
dc.titleDirect AAV-mediated gene delivery to the temporomandibular jointen_HK
dc.typeArticleen_HK
dc.identifier.emailDai, J:en_HK
dc.identifier.emailRabie, ABM: rabie@hku.hken_HK
dc.identifier.authorityDai, J=rp01569en_HK
dc.identifier.authorityRabie, ABM=rp00029en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.2741/2224en_HK
dc.identifier.scopuseid_2-s2.0-34249863696en_HK
dc.identifier.hkuros124250-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34249863696&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume12en_HK
dc.identifier.issue6en_HK
dc.identifier.spage2212en_HK
dc.identifier.epage2220en_HK
dc.identifier.isiWOS:000243745000181-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridDai, J=35387686200en_HK
dc.identifier.scopusauthoridRabie, ABM=7007172734en_HK

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