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Article: Recombinant AAV-mediated VEGF gene therapy induces mandibular condylar growth

TitleRecombinant AAV-mediated VEGF gene therapy induces mandibular condylar growth
Authors
Issue Date2007
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/gt
Citation
Gene Therapy, 2007, v. 14 n. 12, p. 972-980 How to Cite?
AbstractCraniofacial anomalies resulting from impaired growth of mandibular condyles require multidisciplinary interventions, which impose a substantial burden on patients and their families. So far, correcting such deformities with an alternative strategy - gene therapy - is still an uncharted territory. Here, we established an effective in vivo gene delivery system with recombinant adeno-associated virus (rAAV)-mediated vascular endothelial growth factor (VEGF) to enhance mandibular condylar growth. With in situ hybridization, RT-PCR, immunostaining and Western blot, transgene expression was clearly detected in the mandibular condyles during the whole experiment periods. At defined time points, specific osteogenetic markers (alkaline phosphatase and osteocalcin) and chondrogenetic markers (collagen type II and collagen type X) were assessed by means of biochemical analysis and their expression significantly changed from day 30. Proliferation index by proliferating cell nuclear antigen staining showed also a significant increase in cell proliferation. Morphological measurement identified that the size of mandibular condyle significantly increased from day 30. Taken together, rAAV-VEGF was successfully established as an efficient delivery system to induce mandibular condylar growth, which provides the basis for future gene therapy to treat patients with craniofacial deformities.
Persistent Identifierhttp://hdl.handle.net/10722/144271
ISSN
2015 Impact Factor: 3.242
2015 SCImago Journal Rankings: 1.519
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorRabie, ABMen_HK
dc.contributor.authorDai, Jen_HK
dc.contributor.authorXu, Ren_HK
dc.date.accessioned2012-01-20T08:58:38Z-
dc.date.available2012-01-20T08:58:38Z-
dc.date.issued2007en_HK
dc.identifier.citationGene Therapy, 2007, v. 14 n. 12, p. 972-980en_HK
dc.identifier.issn0969-7128en_HK
dc.identifier.urihttp://hdl.handle.net/10722/144271-
dc.description.abstractCraniofacial anomalies resulting from impaired growth of mandibular condyles require multidisciplinary interventions, which impose a substantial burden on patients and their families. So far, correcting such deformities with an alternative strategy - gene therapy - is still an uncharted territory. Here, we established an effective in vivo gene delivery system with recombinant adeno-associated virus (rAAV)-mediated vascular endothelial growth factor (VEGF) to enhance mandibular condylar growth. With in situ hybridization, RT-PCR, immunostaining and Western blot, transgene expression was clearly detected in the mandibular condyles during the whole experiment periods. At defined time points, specific osteogenetic markers (alkaline phosphatase and osteocalcin) and chondrogenetic markers (collagen type II and collagen type X) were assessed by means of biochemical analysis and their expression significantly changed from day 30. Proliferation index by proliferating cell nuclear antigen staining showed also a significant increase in cell proliferation. Morphological measurement identified that the size of mandibular condyle significantly increased from day 30. Taken together, rAAV-VEGF was successfully established as an efficient delivery system to induce mandibular condylar growth, which provides the basis for future gene therapy to treat patients with craniofacial deformities.en_HK
dc.languageengen_US
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/gten_HK
dc.relation.ispartofGene Therapyen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshBlotting, Westernen_HK
dc.subject.meshCell Proliferationen_HK
dc.subject.meshChondrocytes - cytologyen_HK
dc.subject.meshDependovirus - geneticsen_HK
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGene Therapy - methodsen_HK
dc.subject.meshGenetic Engineeringen_HK
dc.subject.meshGenetic Vectors - administration & dosage - geneticsen_HK
dc.subject.meshGreen Fluorescent Proteins - geneticsen_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshIn Situ Hybridizationen_HK
dc.subject.meshMandibular Condyle - growth & development - metabolismen_HK
dc.subject.meshMesenchymal Stem Cells - cytologyen_HK
dc.subject.meshMicroscopy, Fluorescenceen_HK
dc.subject.meshOsteoclasts - cytologyen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRats, Sprague-Dawleyen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.subject.meshTransduction, Genetic - methodsen_HK
dc.subject.meshVascular Endothelial Growth Factor A - analysis - geneticsen_HK
dc.titleRecombinant AAV-mediated VEGF gene therapy induces mandibular condylar growthen_HK
dc.typeArticleen_HK
dc.identifier.emailRabie, ABM:rabie@hku.hken_HK
dc.identifier.emailDai, J:en_HK
dc.identifier.authorityRabie, ABM=rp00029en_HK
dc.identifier.authorityDai, J=rp01569en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1038/sj.gt.3302943en_HK
dc.identifier.pmid17460722-
dc.identifier.scopuseid_2-s2.0-34249873545en_HK
dc.identifier.hkuros143932-
dc.identifier.hkuros135850-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34249873545&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume14en_HK
dc.identifier.issue12en_HK
dc.identifier.spage972en_HK
dc.identifier.epage980en_HK
dc.identifier.eissn1476-5462-
dc.identifier.isiWOS:000247058700006-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridRabie, ABM=7007172734en_HK
dc.identifier.scopusauthoridDai, J=35387686200en_HK
dc.identifier.scopusauthoridXu, R=7402813857en_HK
dc.identifier.citeulike1256194-

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