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Article: Gene therapy to enhance condylar growth using rAAV-VEGF

TitleGene therapy to enhance condylar growth using rAAV-VEGF
Authors
KeywordsGene therapy
Mandibular condylar growth
rAAV
VEGF
Issue Date2008
PublisherE H Angle Education and Research Foundation, Inc. The Journal's web site is located at http://www.angle.org/
Citation
Angle Orthodontist, 2008, v. 78 n. 1, p. 89-94 How to Cite?
AbstractObjective: To test the hypothesis that the introduction of specific vascular growth inducting genes would favorably affect mandibular condylar growth in Sprague-Dawley (SD) rats over a limited experimental period. Therefore, the aim of this study is to examine the effect of gene therapy on condylar growth by means of a morphological assessment. Materials and Methods: Ninety 35-day-old female SD rats were randomly divided into three groups, which received any of the injections of recombinant adeno-associated virus mediated vascular endothelial growth factor (rAAV-VEGF), rAAV mediated enhanced green fluorescence protein (rAAV-eGFP), or phosphate-buffered saline (PBS) into both mandibular condyles. Each group of rats was sacrificed on the following experimental days: 7, 14, 21, 30, and 60. Left halves of the mandibles were isolated and digital pictures were obtained in a standardized manner. Results: The length of condylar process (B-F) as well as mandibular length (A-F) significantly increased on day 30 and continued to increase until the end of the experiment. Moreover, the width of condyle (Q-R) had increased significantly from day 30 and lasted to day 60. Condylar length (C-D) was found to be significantly longer on day 60. Conclusions: Gene therapy with VEGF stimulates condylar growth at will. The rAAV-VEGF is an excellent candidate for future gene therapy to induce mandibular growth. © 2007 by The EH Angle Education and Research Foundation, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/144269
ISSN
2015 Impact Factor: 1.579
2015 SCImago Journal Rankings: 1.254
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorDai, Jen_HK
dc.contributor.authorRabie, ABMen_HK
dc.date.accessioned2012-01-20T08:58:34Z-
dc.date.available2012-01-20T08:58:34Z-
dc.date.issued2008en_HK
dc.identifier.citationAngle Orthodontist, 2008, v. 78 n. 1, p. 89-94en_HK
dc.identifier.issn0003-3219en_HK
dc.identifier.urihttp://hdl.handle.net/10722/144269-
dc.description.abstractObjective: To test the hypothesis that the introduction of specific vascular growth inducting genes would favorably affect mandibular condylar growth in Sprague-Dawley (SD) rats over a limited experimental period. Therefore, the aim of this study is to examine the effect of gene therapy on condylar growth by means of a morphological assessment. Materials and Methods: Ninety 35-day-old female SD rats were randomly divided into three groups, which received any of the injections of recombinant adeno-associated virus mediated vascular endothelial growth factor (rAAV-VEGF), rAAV mediated enhanced green fluorescence protein (rAAV-eGFP), or phosphate-buffered saline (PBS) into both mandibular condyles. Each group of rats was sacrificed on the following experimental days: 7, 14, 21, 30, and 60. Left halves of the mandibles were isolated and digital pictures were obtained in a standardized manner. Results: The length of condylar process (B-F) as well as mandibular length (A-F) significantly increased on day 30 and continued to increase until the end of the experiment. Moreover, the width of condyle (Q-R) had increased significantly from day 30 and lasted to day 60. Condylar length (C-D) was found to be significantly longer on day 60. Conclusions: Gene therapy with VEGF stimulates condylar growth at will. The rAAV-VEGF is an excellent candidate for future gene therapy to induce mandibular growth. © 2007 by The EH Angle Education and Research Foundation, Inc.en_HK
dc.languageengen_US
dc.publisherE H Angle Education and Research Foundation, Inc. The Journal's web site is located at http://www.angle.org/en_HK
dc.relation.ispartofAngle Orthodontisten_HK
dc.subjectGene therapyen_HK
dc.subjectMandibular condylar growthen_HK
dc.subjectrAAVen_HK
dc.subjectVEGFen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCephalometryen_HK
dc.subject.meshDependovirus - geneticsen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshFluorescent Dyes - diagnostic useen_HK
dc.subject.meshGene Therapyen_HK
dc.subject.meshGenetic Vectorsen_HK
dc.subject.meshGreen Fluorescent Proteins - diagnostic useen_HK
dc.subject.meshMandible - anatomy & histology - growth & developmenten_HK
dc.subject.meshMandibular Condyle - anatomy & histology - growth & developmenten_HK
dc.subject.meshMicroinjectionsen_HK
dc.subject.meshRandom Allocationen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRats, Sprague-Dawleyen_HK
dc.subject.meshRecombinant Proteinsen_HK
dc.subject.meshSodium Chlorideen_HK
dc.subject.meshTime Factorsen_HK
dc.subject.meshVascular Endothelial Growth Factor A - genetics - therapeutic useen_HK
dc.titleGene therapy to enhance condylar growth using rAAV-VEGFen_HK
dc.typeArticleen_HK
dc.identifier.emailDai, J:en_HK
dc.identifier.emailRabie, ABM: rabie@hku.hken_HK
dc.identifier.authorityDai, J=rp01569en_HK
dc.identifier.authorityRabie, ABM=rp00029en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.2319/102606-441.1en_HK
dc.identifier.pmid18193964-
dc.identifier.scopuseid_2-s2.0-38349006851en_HK
dc.identifier.hkuros143942-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-38349006851&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume78en_HK
dc.identifier.issue1en_HK
dc.identifier.spage89en_HK
dc.identifier.epage94en_HK
dc.identifier.isiWOS:000252511100014-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridDai, J=35387686200en_HK
dc.identifier.scopusauthoridRabie, ABM=7007172734en_HK

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