Effect Of Recombinant Human Bone Morphogenetic Protein-7 On Proliferation And Differentiation Of Human Periodontal Ligament Cells

Abstract

Objective: To Study The Effect Of Recombinant Human Bone Morphogenetic Protein-7 (Rhbmp-7) On Proliferation And Differentiation Of Human Periodontal Ligament Cells (Hpdlcs) Cultivated In Vitro. Methods: The Hpdlcs Were Tested Vimentin Positive And Ck (Pan) Negative. Different Dosages (50, 100, 200 And 400 Μmol·L-1) Of Rhbmp-7 Were Added In The Cultured Hpdlcs. 1, 3, 5 D After Administration Of Rhbmp-7, The Proliferation And Osteogenic Differentiation Of Hpdlcs Were Examined By Mtt Assay And The Activity Of Alkaline Phosphatase (Alp) Was Detected With Elisa. Results: The Administration Of Rhbmp-7 Significantly Elevated The Proliferation Rate And Differentiation Of Hpdlcs With The Dosage Of 100, 200 And 400 Μmol·L-1 Respectively (P< 0.01), No Difference Was Found Between Different Dosages Groups (P>0.05). Moreover, Rhbmp-7 Significantly Induced The Osteogenic Differentiation Ability With The Increased Expression Level Of Alp And 200 Μmol·L-1 Was The Most Effective Dosage In The Differentiation Ability. Conclusion: Bmp-7 May Participate In The Remodeling Of Periodontal Tissue By Regulating The Proliferation And Osteogenic Differentiation Of Hpdlcs.