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Article: Recombinant Adeno-Associated Virus Serotype 2 (Raav2)-An Efficient Vector For Gene Delivery In Condylar Cartilage, Glenoid Fossa And Tmj Disc In An Experimental Study In Vivo

TitleRecombinant Adeno-Associated Virus Serotype 2 (Raav2)-An Efficient Vector For Gene Delivery In Condylar Cartilage, Glenoid Fossa And Tmj Disc In An Experimental Study In Vivo
Authors
Issue Date2009
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/archoralbio
Citation
Archives Of Oral Biology, 2009, v. 54 n. 10, p. 943-950 How to Cite?
AbstractObjective: To Elaborate Whether Raav2 Can Be Used For Future Tmj Gene Therapy, We Examined The Infection Efficiencies Of Raav2 In Vitro, And The Transgene Expression Pattern Mediated By Raav2 In Glenoid Fossa, Tmj Disc And Condylar Cartilage In Vivo. Materials And Methods: Different Dosages Of Raav2-Egfp (Moi: 5 × 104, 1 × 104, 5 × 103) Were Applied To Primary Cultured Condylar Chondrocytes Of Rats. Infection Efficiencies Were Analysed By Facscalitur At Different Time Points. Vastatin, A Molecule Not Naturally Expressed In Tmj, Was Used As A Reporter For Detection Of Raav2 Mediated Transgene Expression In Vivo. Thirty Sd Rats Were Injected With Either Raav2-Sec-Vastatin (Experimental Group) Or Raav2-Egfp (Control Group) Into Both Sides Of Tmj. They Were Sacrificed At The Indicated Time (7, 14, 21, 30 And 60 Days Of Injection) And The Tmj Samples Were Collected For Rt-Pcr And Immunostaining Analysis. Results: High Dosage (Moi 5 × 104) Of Raav2-Egfp Can Achieve Desirable Transduction Efficiencies In Vitro After 5 Days. Transgene Expression Of Raav-Sec-Vastatin Persisted For About 21 Days In Glenoid Fossa, Around 7 Days In Tmj Disc And At Least 60 Days In Condylar Cartilage In Vivo. In Condylar Cartilage, Transgene Expression Was Found In The Proliferative Layer And Chondroblast Layer (Day 7), Chondrocyte Layer (Day 14), Pre-Hypertrophic And Hypertrophic Layer (Day 21), Hypertrophic Layer And Deep Hypertrophic Layer (Day 30 And 60). Conclusion: Recombinant Aav2 Could Be Considered As A Promising Vector For Gene Therapy In Tmj Which Can Mediate Therapeutic Gene Expression In Glenoid Fossa, Articular Disc And Condylar Cartilage In Vivo. © 2009 Elsevier Ltd. All Rights Reserved.
Persistent Identifierhttp://hdl.handle.net/10722/144264
ISSN
2015 Impact Factor: 1.733
2015 SCImago Journal Rankings: 0.713
ISI Accession Number ID
Funding AgencyGrant Number
UGC 3rd Phase Matching Grant Scheme (SHAC 1st year)-Bone Research20730806.22311.08003.400.01
Funding Information:

Funding: This study was supported by an UGC 3rd Phase Matching Grant Scheme (SHAC 1st year)-Bone Research awarded to Professor Rabie, 20730806.22311.08003.400.01.

References

 

DC FieldValueLanguage
dc.contributor.authorLi, Qen_US
dc.contributor.authorDai, Jen_US
dc.contributor.authorRabie, ABMen_US
dc.date.accessioned2012-01-20T08:58:29Z-
dc.date.available2012-01-20T08:58:29Z-
dc.date.issued2009en_US
dc.identifier.citationArchives Of Oral Biology, 2009, v. 54 n. 10, p. 943-950en_US
dc.identifier.issn0003-9969en_US
dc.identifier.urihttp://hdl.handle.net/10722/144264-
dc.description.abstractObjective: To Elaborate Whether Raav2 Can Be Used For Future Tmj Gene Therapy, We Examined The Infection Efficiencies Of Raav2 In Vitro, And The Transgene Expression Pattern Mediated By Raav2 In Glenoid Fossa, Tmj Disc And Condylar Cartilage In Vivo. Materials And Methods: Different Dosages Of Raav2-Egfp (Moi: 5 × 104, 1 × 104, 5 × 103) Were Applied To Primary Cultured Condylar Chondrocytes Of Rats. Infection Efficiencies Were Analysed By Facscalitur At Different Time Points. Vastatin, A Molecule Not Naturally Expressed In Tmj, Was Used As A Reporter For Detection Of Raav2 Mediated Transgene Expression In Vivo. Thirty Sd Rats Were Injected With Either Raav2-Sec-Vastatin (Experimental Group) Or Raav2-Egfp (Control Group) Into Both Sides Of Tmj. They Were Sacrificed At The Indicated Time (7, 14, 21, 30 And 60 Days Of Injection) And The Tmj Samples Were Collected For Rt-Pcr And Immunostaining Analysis. Results: High Dosage (Moi 5 × 104) Of Raav2-Egfp Can Achieve Desirable Transduction Efficiencies In Vitro After 5 Days. Transgene Expression Of Raav-Sec-Vastatin Persisted For About 21 Days In Glenoid Fossa, Around 7 Days In Tmj Disc And At Least 60 Days In Condylar Cartilage In Vivo. In Condylar Cartilage, Transgene Expression Was Found In The Proliferative Layer And Chondroblast Layer (Day 7), Chondrocyte Layer (Day 14), Pre-Hypertrophic And Hypertrophic Layer (Day 21), Hypertrophic Layer And Deep Hypertrophic Layer (Day 30 And 60). Conclusion: Recombinant Aav2 Could Be Considered As A Promising Vector For Gene Therapy In Tmj Which Can Mediate Therapeutic Gene Expression In Glenoid Fossa, Articular Disc And Condylar Cartilage In Vivo. © 2009 Elsevier Ltd. All Rights Reserved.en_US
dc.languageengen_US
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/archoralbioen_US
dc.relation.ispartofArchives Of Oral Biologyen_US
dc.titleRecombinant Adeno-Associated Virus Serotype 2 (Raav2)-An Efficient Vector For Gene Delivery In Condylar Cartilage, Glenoid Fossa And Tmj Disc In An Experimental Study In Vivoen_US
dc.typeArticleen_US
dc.identifier.authorityDai, J=rp01569en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.archoralbio.2009.07.005en_US
dc.identifier.pmid19683702-
dc.identifier.scopuseid_2-s2.0-69449107544en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-69449107544&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume54en_US
dc.identifier.issue10en_US
dc.identifier.spage943en_US
dc.identifier.epage950en_US
dc.identifier.isiWOS:000270537900008-
dc.identifier.citeulike5469236-

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