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Article: Progesterone Modulates The Proliferation And Differentiation Of Human Periodontal Ligament Cells

TitleProgesterone Modulates The Proliferation And Differentiation Of Human Periodontal Ligament Cells
Authors
KeywordsCell Proliferation
Differentiation
Human Periodontal Ligament Cells
Progesterone
Progesterone Receptor
Issue Date2010
PublisherSpringer New York LLC. The Journal's web site is located at http://link.springer.de/link/service/journals/00223
Citation
Calcified Tissue International, 2010, v. 87, n. 2, p. 158-167 How to Cite?
AbstractHormone Deficiency Has Been Recognized As A Risk Factor For Periodontal Disease In Postmenopausal Women. However, The Anabolic Effects Of Progesterone On Human Periodontal Ligament Cells (Hpdlcs) Are Still Unclear. Therefore, The Objective Of This Study Was To Detect The Expression Of Progesterone Receptor (Pgr) In Hpdlcs And Investigate The Bone-Sparing Effects Of Progesterone. We Detected Pgr Expression In Hpdlcs By Reverse Transcriptase-Polymerase Chain Reaction And Immunocytochemistry. After Progesterone Stimulation, The Percentage Of Hpdlcs Entering The S + G2m Phase Of The Cell Cycle Increased Significantly, Accompanied By An Increased Cell Growth Curve. In Both Basic Culture Medium And Osteogenic Medium, Progesterone Activated Alkaline Phosphatase-Positive Cells And Alizarin Red-Positive Nodules. Moreover, Mineralization-Related Markers Were Up-Regulated By Progesterone In Both Time-Dependent And Dose-Dependent Manners. In Contrast, These Effects Of Progesterone Were Blocked By The Pgr Antagonist (Ru486). Our Results Demonstrated That The Pgr Is Expressed In Hpdlcs At The Gene And Protein Level, And That Progesterone Can Stimulate The Proliferation And Differentiation Of The Hpdlcs. These Findings Suggest That Progesterone May Play A Significant Role In Osteoblastic Function Of Hpdlcs And May Influence The Maintenance Of Alveolar Bone Mass. © 2010 Springer Science+Business Media, Llc.
Persistent Identifierhttp://hdl.handle.net/10722/144258
ISSN
2015 Impact Factor: 3.052
2015 SCImago Journal Rankings: 1.166
ISI Accession Number ID
Funding AgencyGrant Number
Nature Science Foundation of China30872913
Funding Information:

This study was supported by Nature Science Foundation of China (grant 30872913). We thank Dr. Jiaxing Zhou for generously providing the MCF-7 cell line. We also acknowledge the guidance and advice of Dr. Liang Tang and Dr. Dongyang Ma.

 

DC FieldValueLanguage
dc.contributor.authorYuan, Gen_US
dc.contributor.authorCai, Cen_US
dc.contributor.authorDai, Jen_US
dc.contributor.authorLiu, Yen_US
dc.contributor.authorZhang, Ren_US
dc.contributor.authorDai, Yen_US
dc.contributor.authorWen, Len_US
dc.contributor.authorDing, Yen_US
dc.date.accessioned2012-01-20T08:58:26Z-
dc.date.available2012-01-20T08:58:26Z-
dc.date.issued2010en_US
dc.identifier.citationCalcified Tissue International, 2010, v. 87, n. 2, p. 158-167en_US
dc.identifier.issn0171-967Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/144258-
dc.description.abstractHormone Deficiency Has Been Recognized As A Risk Factor For Periodontal Disease In Postmenopausal Women. However, The Anabolic Effects Of Progesterone On Human Periodontal Ligament Cells (Hpdlcs) Are Still Unclear. Therefore, The Objective Of This Study Was To Detect The Expression Of Progesterone Receptor (Pgr) In Hpdlcs And Investigate The Bone-Sparing Effects Of Progesterone. We Detected Pgr Expression In Hpdlcs By Reverse Transcriptase-Polymerase Chain Reaction And Immunocytochemistry. After Progesterone Stimulation, The Percentage Of Hpdlcs Entering The S + G2m Phase Of The Cell Cycle Increased Significantly, Accompanied By An Increased Cell Growth Curve. In Both Basic Culture Medium And Osteogenic Medium, Progesterone Activated Alkaline Phosphatase-Positive Cells And Alizarin Red-Positive Nodules. Moreover, Mineralization-Related Markers Were Up-Regulated By Progesterone In Both Time-Dependent And Dose-Dependent Manners. In Contrast, These Effects Of Progesterone Were Blocked By The Pgr Antagonist (Ru486). Our Results Demonstrated That The Pgr Is Expressed In Hpdlcs At The Gene And Protein Level, And That Progesterone Can Stimulate The Proliferation And Differentiation Of The Hpdlcs. These Findings Suggest That Progesterone May Play A Significant Role In Osteoblastic Function Of Hpdlcs And May Influence The Maintenance Of Alveolar Bone Mass. © 2010 Springer Science+Business Media, Llc.en_US
dc.languageengen_US
dc.publisherSpringer New York LLC. The Journal's web site is located at http://link.springer.de/link/service/journals/00223en_US
dc.relation.ispartofCalcified Tissue Internationalen_US
dc.subjectCell Proliferationen_US
dc.subjectDifferentiationen_US
dc.subjectHuman Periodontal Ligament Cellsen_US
dc.subjectProgesteroneen_US
dc.subjectProgesterone Receptoren_US
dc.titleProgesterone Modulates The Proliferation And Differentiation Of Human Periodontal Ligament Cellsen_US
dc.typeArticleen_US
dc.identifier.authorityDai, J=rp01569en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s00223-010-9377-9en_US
dc.identifier.pmid20532879-
dc.identifier.scopuseid_2-s2.0-79952108496en_US
dc.identifier.spage1en_US
dc.identifier.epage10en_US
dc.identifier.eissn1432-0827-
dc.identifier.isiWOS:000280062200007-
dc.identifier.citeulike7298384-

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