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Article: Effect Of The Cyclic Stretch On The Expression Of Osteogenesis Genes In Human Periodontal Ligament Cells

TitleEffect Of The Cyclic Stretch On The Expression Of Osteogenesis Genes In Human Periodontal Ligament Cells
Authors
KeywordsCdna Microarray
Differentiation
Human Periodontal Ligament Cells
Mechanical Strain
Issue Date2012
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/gene
Citation
Gene, 2012, v. 491 n. 2, p. 187-193 How to Cite?
Abstract
Periodontal Ligament Cells Can Potentially Differentiate Into Osteoblast-Like Cells And Influence The Remodeling Of Periodontal Tissues Under Mechanical Strain Conditions. In The Present Study, Gene Chip Technology Was Adopted To Investigate The Effect Of The Cyclic Stretch On The Expression Of Osteogenic-Related Genes In Human Periodontal Ligament Cells (Hpdlcs). Cultured Hpdlcs Were Subjected To 12% Elongation Cyclic Stretch For 24. H Using A Flexercell Strain Unit, And Then Gearray Q Series Human Osteogenesis Gene Expression Profile Chip With 96 Spot Array Numbers Was Used To Conduct Parallel Analyses On The Change Of The Related Gene Expression In The Osteogenic Differentiation Of Hpdlcs Stimulated By Cyclic Stretch. The Results Show That After The Hpdlcs Were Stimulated By The Cyclic Stretch, The Expression Of 21 Osteogenic-Related Genes Was Significantly Upregulated, Including 10 Growth Factor Genes And Their Associated Molecules, 10 Extracellular Matrix Genes And Their Associated Proteins, And 1 Cell Adhesion Molecule. Two Genes Were Significantly Downregulated, Including One Growth Factor Gene And One Cell Adhesion Molecule. Then The Expressions Of 10 Candidate Genes Were Validated Using Real-Time Rt-Pcr. These Results Indicate That Cyclic Stretch With 12% Deformation Can Stimulate Or Inhibit Some Gene Expression Which Was Associated With The Process Of Hpdlcs Differentiation. © 2011 Elsevier B.V.
Persistent Identifierhttp://hdl.handle.net/10722/144254
ISSN
2013 Impact Factor: 2.082
ISI Accession Number ID
Funding AgencyGrant Number
National Natural Science Foundation of China30970697
Funding Information:

This work was supported by the National Natural Science Foundation of China (No. 30970697).

References

 

DC FieldValueLanguage
dc.contributor.authorLiu, Men_US
dc.contributor.authorDai, Jen_US
dc.contributor.authorLin, Yen_US
dc.contributor.authorYang, Len_US
dc.contributor.authorDong, Hen_US
dc.contributor.authorLi, Yen_US
dc.contributor.authorDing, Yen_US
dc.contributor.authorDuan, Yen_US
dc.date.accessioned2012-01-20T08:58:19Z-
dc.date.available2012-01-20T08:58:19Z-
dc.date.issued2012en_US
dc.identifier.citationGene, 2012, v. 491 n. 2, p. 187-193en_US
dc.identifier.issn0378-1119en_US
dc.identifier.urihttp://hdl.handle.net/10722/144254-
dc.description.abstractPeriodontal Ligament Cells Can Potentially Differentiate Into Osteoblast-Like Cells And Influence The Remodeling Of Periodontal Tissues Under Mechanical Strain Conditions. In The Present Study, Gene Chip Technology Was Adopted To Investigate The Effect Of The Cyclic Stretch On The Expression Of Osteogenic-Related Genes In Human Periodontal Ligament Cells (Hpdlcs). Cultured Hpdlcs Were Subjected To 12% Elongation Cyclic Stretch For 24. H Using A Flexercell Strain Unit, And Then Gearray Q Series Human Osteogenesis Gene Expression Profile Chip With 96 Spot Array Numbers Was Used To Conduct Parallel Analyses On The Change Of The Related Gene Expression In The Osteogenic Differentiation Of Hpdlcs Stimulated By Cyclic Stretch. The Results Show That After The Hpdlcs Were Stimulated By The Cyclic Stretch, The Expression Of 21 Osteogenic-Related Genes Was Significantly Upregulated, Including 10 Growth Factor Genes And Their Associated Molecules, 10 Extracellular Matrix Genes And Their Associated Proteins, And 1 Cell Adhesion Molecule. Two Genes Were Significantly Downregulated, Including One Growth Factor Gene And One Cell Adhesion Molecule. Then The Expressions Of 10 Candidate Genes Were Validated Using Real-Time Rt-Pcr. These Results Indicate That Cyclic Stretch With 12% Deformation Can Stimulate Or Inhibit Some Gene Expression Which Was Associated With The Process Of Hpdlcs Differentiation. © 2011 Elsevier B.V.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/geneen_US
dc.relation.ispartofGeneen_US
dc.subjectCdna Microarrayen_US
dc.subjectDifferentiationen_US
dc.subjectHuman Periodontal Ligament Cellsen_US
dc.subjectMechanical Strainen_US
dc.titleEffect Of The Cyclic Stretch On The Expression Of Osteogenesis Genes In Human Periodontal Ligament Cellsen_US
dc.typeArticleen_US
dc.identifier.authorityDai, J=rp01569en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.gene.2011.09.031en_US
dc.identifier.pmid22019432-
dc.identifier.scopuseid_2-s2.0-81455158392en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-81455158392&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume491en_US
dc.identifier.issue2en_US
dc.identifier.spage187en_US
dc.identifier.epage193en_US
dc.identifier.eissn1879-0038-
dc.identifier.isiWOS:000298535400013-
dc.identifier.citeulike9947125-

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