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Article: Effect Of The Cyclic Stretch On The Expression Of Osteogenesis Genes In Human Periodontal Ligament Cells
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TitleEffect Of The Cyclic Stretch On The Expression Of Osteogenesis Genes In Human Periodontal Ligament Cells
 
AuthorsLiu, M1
Dai, J1
Lin, Y1
Yang, L1
Dong, H1
Li, Y1
Ding, Y1
Duan, Y1
 
KeywordsCdna Microarray
Differentiation
Human Periodontal Ligament Cells
Mechanical Strain
 
Issue Date2012
 
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/gene
 
CitationGene, 2012, v. 491 n. 2, p. 187-193 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.gene.2011.09.031
 
AbstractPeriodontal Ligament Cells Can Potentially Differentiate Into Osteoblast-Like Cells And Influence The Remodeling Of Periodontal Tissues Under Mechanical Strain Conditions. In The Present Study, Gene Chip Technology Was Adopted To Investigate The Effect Of The Cyclic Stretch On The Expression Of Osteogenic-Related Genes In Human Periodontal Ligament Cells (Hpdlcs). Cultured Hpdlcs Were Subjected To 12% Elongation Cyclic Stretch For 24. H Using A Flexercell Strain Unit, And Then Gearray Q Series Human Osteogenesis Gene Expression Profile Chip With 96 Spot Array Numbers Was Used To Conduct Parallel Analyses On The Change Of The Related Gene Expression In The Osteogenic Differentiation Of Hpdlcs Stimulated By Cyclic Stretch. The Results Show That After The Hpdlcs Were Stimulated By The Cyclic Stretch, The Expression Of 21 Osteogenic-Related Genes Was Significantly Upregulated, Including 10 Growth Factor Genes And Their Associated Molecules, 10 Extracellular Matrix Genes And Their Associated Proteins, And 1 Cell Adhesion Molecule. Two Genes Were Significantly Downregulated, Including One Growth Factor Gene And One Cell Adhesion Molecule. Then The Expressions Of 10 Candidate Genes Were Validated Using Real-Time Rt-Pcr. These Results Indicate That Cyclic Stretch With 12% Deformation Can Stimulate Or Inhibit Some Gene Expression Which Was Associated With The Process Of Hpdlcs Differentiation. © 2011 Elsevier B.V.
 
ISSN0378-1119
2013 Impact Factor: 2.082
 
DOIhttp://dx.doi.org/10.1016/j.gene.2011.09.031
 
ISI Accession Number IDWOS:000298535400013
Funding AgencyGrant Number
National Natural Science Foundation of China30970697
Funding Information:

This work was supported by the National Natural Science Foundation of China (No. 30970697).

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorLiu, M
 
dc.contributor.authorDai, J
 
dc.contributor.authorLin, Y
 
dc.contributor.authorYang, L
 
dc.contributor.authorDong, H
 
dc.contributor.authorLi, Y
 
dc.contributor.authorDing, Y
 
dc.contributor.authorDuan, Y
 
dc.date.accessioned2012-01-20T08:58:19Z
 
dc.date.available2012-01-20T08:58:19Z
 
dc.date.issued2012
 
dc.description.abstractPeriodontal Ligament Cells Can Potentially Differentiate Into Osteoblast-Like Cells And Influence The Remodeling Of Periodontal Tissues Under Mechanical Strain Conditions. In The Present Study, Gene Chip Technology Was Adopted To Investigate The Effect Of The Cyclic Stretch On The Expression Of Osteogenic-Related Genes In Human Periodontal Ligament Cells (Hpdlcs). Cultured Hpdlcs Were Subjected To 12% Elongation Cyclic Stretch For 24. H Using A Flexercell Strain Unit, And Then Gearray Q Series Human Osteogenesis Gene Expression Profile Chip With 96 Spot Array Numbers Was Used To Conduct Parallel Analyses On The Change Of The Related Gene Expression In The Osteogenic Differentiation Of Hpdlcs Stimulated By Cyclic Stretch. The Results Show That After The Hpdlcs Were Stimulated By The Cyclic Stretch, The Expression Of 21 Osteogenic-Related Genes Was Significantly Upregulated, Including 10 Growth Factor Genes And Their Associated Molecules, 10 Extracellular Matrix Genes And Their Associated Proteins, And 1 Cell Adhesion Molecule. Two Genes Were Significantly Downregulated, Including One Growth Factor Gene And One Cell Adhesion Molecule. Then The Expressions Of 10 Candidate Genes Were Validated Using Real-Time Rt-Pcr. These Results Indicate That Cyclic Stretch With 12% Deformation Can Stimulate Or Inhibit Some Gene Expression Which Was Associated With The Process Of Hpdlcs Differentiation. © 2011 Elsevier B.V.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationGene, 2012, v. 491 n. 2, p. 187-193 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.gene.2011.09.031
 
dc.identifier.citeulike9947125
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.gene.2011.09.031
 
dc.identifier.eissn1879-0038
 
dc.identifier.epage193
 
dc.identifier.isiWOS:000298535400013
Funding AgencyGrant Number
National Natural Science Foundation of China30970697
Funding Information:

This work was supported by the National Natural Science Foundation of China (No. 30970697).

 
dc.identifier.issn0378-1119
2013 Impact Factor: 2.082
 
dc.identifier.issue2
 
dc.identifier.pmid22019432
 
dc.identifier.scopuseid_2-s2.0-81455158392
 
dc.identifier.spage187
 
dc.identifier.urihttp://hdl.handle.net/10722/144254
 
dc.identifier.volume491
 
dc.languageeng
 
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/gene
 
dc.relation.ispartofGene
 
dc.relation.referencesReferences in Scopus
 
dc.subjectCdna Microarray
 
dc.subjectDifferentiation
 
dc.subjectHuman Periodontal Ligament Cells
 
dc.subjectMechanical Strain
 
dc.titleEffect Of The Cyclic Stretch On The Expression Of Osteogenesis Genes In Human Periodontal Ligament Cells
 
dc.typeArticle
 
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<contributor.author>Dong, H</contributor.author>
<contributor.author>Li, Y</contributor.author>
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Author Affiliations
  1. The Fourth Military Medical University