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Article: Bacterial lipopolysaccharides variably modulate in vitro biofilm formation of Candida species
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TitleBacterial lipopolysaccharides variably modulate in vitro biofilm formation of Candida species
 
AuthorsBandara, HMHN2
Lam, OLT1
Watt, RM2
Jin, LJ1
Samaranayake, LP2
 
Issue Date2010
 
PublisherSociety for General Microbiology. The Journal's web site is located at http://jmm.sgmjournals.org
 
CitationJournal Of Medical Microbiology, 2010, v. 59 n. 10, p. 1225-1234 [How to Cite?]
DOI: http://dx.doi.org/10.1099/jmm.0.021832-0
 
AbstractThe objective of this study was to evaluate the effect of the bacterial endotoxin LPS on Candida biofilm formation in vitro. The effect of the LPS of Pseudomonas aeruginosa, Klebsiella pneumoniae, Serratia marcescens and Salmonella typhimurium on six different species of Candida, comprising Candida albicans ATCC 90028, Candida glabrata ATCC 90030, Candida krusei ATCC 6258, Candida tropicalis ATCC 13803, Candida parapsilosis ATCC 22019 and Candida dubliniensis MYA 646, was studied using a standard biofilm assay. The metabolic activity of in vitro Candida biofilms treated with LPS at 90 min, 24 h and 48 h was quantified by XTT reduction assay. Viable biofilm-forming cells were qualitatively analysed using confocal laser scanning microscopy (CLSM), while scanning electron microscopy (SEM) was employed to visualize the biofilm structure. Initially, adhesion of C. albicans was significantly stimulated by Pseudomonas and Klebsiella LPS. A significant inhibition of Candida adhesion was noted for the following combinations: C. glabrata with Pseudomonas LPS, C. tropicalis with Serratia LPS, and C. glabrata, C. parapsilosis or C. dubliniensis with Salmonella LPS (P<0.05). After 24 h of incubation, a significant stimulation of initial colonization was noted for the following combinations: C. albicans/C. glabrata with Klebsiella LPS, C. glabrata/C. tropicalis/C. krusei with Salmonella LPS. In contrast, a significant inhibition of biofilm formation was observed in C. glabrata/C. dubliniensis/C. krusei with Pseudomonas LPS, C. krusei with Serratia LPS, C. dubliniensis with Klebsiella LPS and C. parapsilosis/C. dubliniensis /C. krusei with Salmonella LPS (P<0.05). On further incubation for 48 h, a significant enhancement of biofilm maturation was noted for the following combinations: C. glabrata/C. tropicalis with Serratia LPS, C. dubliniensis with Klebsiella LPS and C. glabrata with Salmonella LPS, and a significant retardation was noted for C. parapsilosis/C. dubliniensis/C. krusei with Pseudomonas LPS, C. tropicalis with Serratia LPS, C. glabrata/C. parapsilosis/C. dubliniensis with Klebsiella LPS and C. dubliniensis with Salmonella LPS (P<0.05). These findings were confirmed by SEM and CLSM analyses. In general, the inhibition of the biofilm development of LPS-treated Candida spp. was accompanied by a scanty architecture with a reduced numbers of cells compared with the profuse and densely colonized control biofilms. These data are indicative that bacterial LPSs modulate in vitro Candida biofilm formation in a species-specific and time-dependent manner. The clinical and the biological relevance of these findings have yet to be explored. © 2010 SGM.
 
ISSN0022-2615
2013 Impact Factor: 2.266
2013 SCImago Journal Rankings: 1.025
 
DOIhttp://dx.doi.org/10.1099/jmm.0.021832-0
 
ISI Accession Number IDWOS:000283004700015
Funding AgencyGrant Number
University of Hong KongCERG HKU 7624/06M
Funding Information:

Authors would like to acknowledge Ms Joyce Yau and Mr Simon Lee for technical help This study was supported by the University of Hong Kong, grant number CERG HKU 7624/06M.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorBandara, HMHN
 
dc.contributor.authorLam, OLT
 
dc.contributor.authorWatt, RM
 
dc.contributor.authorJin, LJ
 
dc.contributor.authorSamaranayake, LP
 
dc.date.accessioned2011-12-22T02:50:07Z
 
dc.date.available2011-12-22T02:50:07Z
 
dc.date.issued2010
 
dc.description.abstractThe objective of this study was to evaluate the effect of the bacterial endotoxin LPS on Candida biofilm formation in vitro. The effect of the LPS of Pseudomonas aeruginosa, Klebsiella pneumoniae, Serratia marcescens and Salmonella typhimurium on six different species of Candida, comprising Candida albicans ATCC 90028, Candida glabrata ATCC 90030, Candida krusei ATCC 6258, Candida tropicalis ATCC 13803, Candida parapsilosis ATCC 22019 and Candida dubliniensis MYA 646, was studied using a standard biofilm assay. The metabolic activity of in vitro Candida biofilms treated with LPS at 90 min, 24 h and 48 h was quantified by XTT reduction assay. Viable biofilm-forming cells were qualitatively analysed using confocal laser scanning microscopy (CLSM), while scanning electron microscopy (SEM) was employed to visualize the biofilm structure. Initially, adhesion of C. albicans was significantly stimulated by Pseudomonas and Klebsiella LPS. A significant inhibition of Candida adhesion was noted for the following combinations: C. glabrata with Pseudomonas LPS, C. tropicalis with Serratia LPS, and C. glabrata, C. parapsilosis or C. dubliniensis with Salmonella LPS (P<0.05). After 24 h of incubation, a significant stimulation of initial colonization was noted for the following combinations: C. albicans/C. glabrata with Klebsiella LPS, C. glabrata/C. tropicalis/C. krusei with Salmonella LPS. In contrast, a significant inhibition of biofilm formation was observed in C. glabrata/C. dubliniensis/C. krusei with Pseudomonas LPS, C. krusei with Serratia LPS, C. dubliniensis with Klebsiella LPS and C. parapsilosis/C. dubliniensis /C. krusei with Salmonella LPS (P<0.05). On further incubation for 48 h, a significant enhancement of biofilm maturation was noted for the following combinations: C. glabrata/C. tropicalis with Serratia LPS, C. dubliniensis with Klebsiella LPS and C. glabrata with Salmonella LPS, and a significant retardation was noted for C. parapsilosis/C. dubliniensis/C. krusei with Pseudomonas LPS, C. tropicalis with Serratia LPS, C. glabrata/C. parapsilosis/C. dubliniensis with Klebsiella LPS and C. dubliniensis with Salmonella LPS (P<0.05). These findings were confirmed by SEM and CLSM analyses. In general, the inhibition of the biofilm development of LPS-treated Candida spp. was accompanied by a scanty architecture with a reduced numbers of cells compared with the profuse and densely colonized control biofilms. These data are indicative that bacterial LPSs modulate in vitro Candida biofilm formation in a species-specific and time-dependent manner. The clinical and the biological relevance of these findings have yet to be explored. © 2010 SGM.
 
dc.description.naturelink_to_OA_fulltext
 
dc.identifier.citationJournal Of Medical Microbiology, 2010, v. 59 n. 10, p. 1225-1234 [How to Cite?]
DOI: http://dx.doi.org/10.1099/jmm.0.021832-0
 
dc.identifier.doihttp://dx.doi.org/10.1099/jmm.0.021832-0
 
dc.identifier.eissn1473-5644
 
dc.identifier.epage1234
 
dc.identifier.hkuros174433
 
dc.identifier.hkuros198198
 
dc.identifier.isiWOS:000283004700015
Funding AgencyGrant Number
University of Hong KongCERG HKU 7624/06M
Funding Information:

Authors would like to acknowledge Ms Joyce Yau and Mr Simon Lee for technical help This study was supported by the University of Hong Kong, grant number CERG HKU 7624/06M.

 
dc.identifier.issn0022-2615
2013 Impact Factor: 2.266
2013 SCImago Journal Rankings: 1.025
 
dc.identifier.issue10
 
dc.identifier.pmid20576747
 
dc.identifier.scopuseid_2-s2.0-77957307594
 
dc.identifier.spage1225
 
dc.identifier.urihttp://hdl.handle.net/10722/143973
 
dc.identifier.volume59
 
dc.languageeng
 
dc.publisherSociety for General Microbiology. The Journal's web site is located at http://jmm.sgmjournals.org
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofJournal of Medical Microbiology
 
dc.relation.referencesReferences in Scopus
 
dc.rightsJournal of Medical Microbiology. Copyright © Society for General Microbiology.
 
dc.subject.meshBiofilms - growth and development
 
dc.subject.meshCandida - growth and development - metabolism - physiology
 
dc.subject.meshCell Wall - chemistry
 
dc.subject.meshGram-Negative Bacteria - chemistry
 
dc.subject.meshLipopolysaccharides - isolation and purification - metabolism
 
dc.titleBacterial lipopolysaccharides variably modulate in vitro biofilm formation of Candida species
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong
  2. Prince Philip Dental Hospital