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Article: Bacterial lipopolysaccharides variably modulate in vitro biofilm formation of Candida species

TitleBacterial lipopolysaccharides variably modulate in vitro biofilm formation of Candida species
Authors
Issue Date2010
PublisherSociety for General Microbiology. The Journal's web site is located at http://jmm.sgmjournals.org
Citation
Journal Of Medical Microbiology, 2010, v. 59 n. 10, p. 1225-1234 How to Cite?
AbstractThe objective of this study was to evaluate the effect of the bacterial endotoxin LPS on Candida biofilm formation in vitro. The effect of the LPS of Pseudomonas aeruginosa, Klebsiella pneumoniae, Serratia marcescens and Salmonella typhimurium on six different species of Candida, comprising Candida albicans ATCC 90028, Candida glabrata ATCC 90030, Candida krusei ATCC 6258, Candida tropicalis ATCC 13803, Candida parapsilosis ATCC 22019 and Candida dubliniensis MYA 646, was studied using a standard biofilm assay. The metabolic activity of in vitro Candida biofilms treated with LPS at 90 min, 24 h and 48 h was quantified by XTT reduction assay. Viable biofilm-forming cells were qualitatively analysed using confocal laser scanning microscopy (CLSM), while scanning electron microscopy (SEM) was employed to visualize the biofilm structure. Initially, adhesion of C. albicans was significantly stimulated by Pseudomonas and Klebsiella LPS. A significant inhibition of Candida adhesion was noted for the following combinations: C. glabrata with Pseudomonas LPS, C. tropicalis with Serratia LPS, and C. glabrata, C. parapsilosis or C. dubliniensis with Salmonella LPS (P<0.05). After 24 h of incubation, a significant stimulation of initial colonization was noted for the following combinations: C. albicans/C. glabrata with Klebsiella LPS, C. glabrata/C. tropicalis/C. krusei with Salmonella LPS. In contrast, a significant inhibition of biofilm formation was observed in C. glabrata/C. dubliniensis/C. krusei with Pseudomonas LPS, C. krusei with Serratia LPS, C. dubliniensis with Klebsiella LPS and C. parapsilosis/C. dubliniensis /C. krusei with Salmonella LPS (P<0.05). On further incubation for 48 h, a significant enhancement of biofilm maturation was noted for the following combinations: C. glabrata/C. tropicalis with Serratia LPS, C. dubliniensis with Klebsiella LPS and C. glabrata with Salmonella LPS, and a significant retardation was noted for C. parapsilosis/C. dubliniensis/C. krusei with Pseudomonas LPS, C. tropicalis with Serratia LPS, C. glabrata/C. parapsilosis/C. dubliniensis with Klebsiella LPS and C. dubliniensis with Salmonella LPS (P<0.05). These findings were confirmed by SEM and CLSM analyses. In general, the inhibition of the biofilm development of LPS-treated Candida spp. was accompanied by a scanty architecture with a reduced numbers of cells compared with the profuse and densely colonized control biofilms. These data are indicative that bacterial LPSs modulate in vitro Candida biofilm formation in a species-specific and time-dependent manner. The clinical and the biological relevance of these findings have yet to be explored. © 2010 SGM.
Persistent Identifierhttp://hdl.handle.net/10722/143973
ISSN
2015 Impact Factor: 2.269
2015 SCImago Journal Rankings: 1.060
ISI Accession Number ID
Funding AgencyGrant Number
University of Hong KongCERG HKU 7624/06M
Funding Information:

Authors would like to acknowledge Ms Joyce Yau and Mr Simon Lee for technical help This study was supported by the University of Hong Kong, grant number CERG HKU 7624/06M.

References

 

DC FieldValueLanguage
dc.contributor.authorBandara, HMHNen_HK
dc.contributor.authorLam, OLTen_HK
dc.contributor.authorWatt, RMen_HK
dc.contributor.authorJin, LJen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.date.accessioned2011-12-22T02:50:07Z-
dc.date.available2011-12-22T02:50:07Z-
dc.date.issued2010en_HK
dc.identifier.citationJournal Of Medical Microbiology, 2010, v. 59 n. 10, p. 1225-1234en_HK
dc.identifier.issn0022-2615en_HK
dc.identifier.urihttp://hdl.handle.net/10722/143973-
dc.description.abstractThe objective of this study was to evaluate the effect of the bacterial endotoxin LPS on Candida biofilm formation in vitro. The effect of the LPS of Pseudomonas aeruginosa, Klebsiella pneumoniae, Serratia marcescens and Salmonella typhimurium on six different species of Candida, comprising Candida albicans ATCC 90028, Candida glabrata ATCC 90030, Candida krusei ATCC 6258, Candida tropicalis ATCC 13803, Candida parapsilosis ATCC 22019 and Candida dubliniensis MYA 646, was studied using a standard biofilm assay. The metabolic activity of in vitro Candida biofilms treated with LPS at 90 min, 24 h and 48 h was quantified by XTT reduction assay. Viable biofilm-forming cells were qualitatively analysed using confocal laser scanning microscopy (CLSM), while scanning electron microscopy (SEM) was employed to visualize the biofilm structure. Initially, adhesion of C. albicans was significantly stimulated by Pseudomonas and Klebsiella LPS. A significant inhibition of Candida adhesion was noted for the following combinations: C. glabrata with Pseudomonas LPS, C. tropicalis with Serratia LPS, and C. glabrata, C. parapsilosis or C. dubliniensis with Salmonella LPS (P<0.05). After 24 h of incubation, a significant stimulation of initial colonization was noted for the following combinations: C. albicans/C. glabrata with Klebsiella LPS, C. glabrata/C. tropicalis/C. krusei with Salmonella LPS. In contrast, a significant inhibition of biofilm formation was observed in C. glabrata/C. dubliniensis/C. krusei with Pseudomonas LPS, C. krusei with Serratia LPS, C. dubliniensis with Klebsiella LPS and C. parapsilosis/C. dubliniensis /C. krusei with Salmonella LPS (P<0.05). On further incubation for 48 h, a significant enhancement of biofilm maturation was noted for the following combinations: C. glabrata/C. tropicalis with Serratia LPS, C. dubliniensis with Klebsiella LPS and C. glabrata with Salmonella LPS, and a significant retardation was noted for C. parapsilosis/C. dubliniensis/C. krusei with Pseudomonas LPS, C. tropicalis with Serratia LPS, C. glabrata/C. parapsilosis/C. dubliniensis with Klebsiella LPS and C. dubliniensis with Salmonella LPS (P<0.05). These findings were confirmed by SEM and CLSM analyses. In general, the inhibition of the biofilm development of LPS-treated Candida spp. was accompanied by a scanty architecture with a reduced numbers of cells compared with the profuse and densely colonized control biofilms. These data are indicative that bacterial LPSs modulate in vitro Candida biofilm formation in a species-specific and time-dependent manner. The clinical and the biological relevance of these findings have yet to be explored. © 2010 SGM.en_HK
dc.languageengen_US
dc.publisherSociety for General Microbiology. The Journal's web site is located at http://jmm.sgmjournals.orgen_HK
dc.relation.ispartofJournal of Medical Microbiologyen_HK
dc.rightsJournal of Medical Microbiology. Copyright © Society for General Microbiology.-
dc.subject.meshBiofilms - growth and development-
dc.subject.meshCandida - growth and development - metabolism - physiology-
dc.subject.meshCell Wall - chemistry-
dc.subject.meshGram-Negative Bacteria - chemistry-
dc.subject.meshLipopolysaccharides - isolation and purification - metabolism-
dc.titleBacterial lipopolysaccharides variably modulate in vitro biofilm formation of Candida speciesen_HK
dc.typeArticleen_HK
dc.identifier.emailLam, OLT:ottolam@hku.hken_HK
dc.identifier.emailWatt, RM:rmwatt@hku.hken_HK
dc.identifier.emailJin, LJ:ljjin@hkucc.hku.hken_HK
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_HK
dc.identifier.authorityLam, OLT=rp01567en_HK
dc.identifier.authorityWatt, RM=rp00043en_HK
dc.identifier.authorityJin, LJ=rp00028en_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1099/jmm.0.021832-0en_HK
dc.identifier.pmid20576747-
dc.identifier.scopuseid_2-s2.0-77957307594en_HK
dc.identifier.hkuros174433-
dc.identifier.hkuros198198-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77957307594&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume59en_HK
dc.identifier.issue10en_HK
dc.identifier.spage1225en_HK
dc.identifier.epage1234en_HK
dc.identifier.eissn1473-5644-
dc.identifier.isiWOS:000283004700015-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridBandara, HMHN=35721385900en_HK
dc.identifier.scopusauthoridLam, OLT=36572015600en_HK
dc.identifier.scopusauthoridWatt, RM=7102907536en_HK
dc.identifier.scopusauthoridJin, LJ=7403328850en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK

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