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Conference Paper: The immunomodulatory properties of glycodelin-A on human monocyte and macrophage

TitleThe immunomodulatory properties of glycodelin-A on human monocyte and macrophage
Authors
Issue Date2011
PublisherSociety for the Study of Reproduction.
Citation
The 44th Annual Meeting of the Society for the Study of Reproduction (SSR 2011), Portland, OR., 31 July-4 August 2011. In Abstracts of the 44th SSR, 2011, p. 167, abstract no. 711 How to Cite?
AbstractMacrophages contribute to 20-30% of the overall population of leukocytes at the maternal-fetal interface. They are in close proximity with the trophoblast which take part in remodeling of placental tissue, clearing apoptotic cells and controlling trophoblast functions. Glycodelin isoforms comprise a group of secretory glycoproteins with four well defined glycoforms, each with a distinct glycosylation pattern. Glycodelin-A (GdA) is a uterine isoform with glycosylation-dependent immunomodulatory activities that contributes to feto-maternal defense. It is abundantly synthesized in the decidua during the first trimester of pregnancy. In the present study, we hypothesized that GdA may exert immunoregulatory activities on monocyte/macrophage and promotes their development into unique phenotypes for maintaining pregnancy. Monocytic cell line THP-1 and monocytes extracted from human female peripheral blood were used as study model. Macrophages were obtained from peripheral blood or by in vitro differentiation of the monocytes using granulocyte-macrophage colony-stimulating factor. Our results showed that GdA did not affect the proliferation and viability of monocytes and macrophages. GdA was demonstrated to up-regulate the interleukin (IL)-6 secretions in macrophage and monocyte as determined by ELISA and flow cytometry. Suppression of extracellular signal-regulated kinases (ERK) signaling abolished the stimulatory effect of GdA, indicating that GdA increased IL-6 production in monocytes/macrophages via the ERK activation. Interestingly, it has been reported that IL-6 is essential for the process of implantation, trophoblast invasion and embryo development. The inclusion of GdA also increased the indoleamine 2,3-dioxyenase (IDO) expression of macrophage, which subsequently inhibited the lymphocyte proliferation in a coculture study. IDO is a marker of decidual macrophage with immunosuppressive activity through depriving the access to tryptophan by immune cells. Taken together, the present data propose a novel role of GdA in modulating the cytokine profile and development of monocytes/macrophages in order to facilitate human pregnancy.
DescriptionConference Theme: Reproduction and the World’s Future
Poster Session B - Reproductive immunology: abstract no. 711
Persistent Identifierhttp://hdl.handle.net/10722/143942

 

DC FieldValueLanguage
dc.contributor.authorLam, EYFen_US
dc.contributor.authorLee, CLen_US
dc.contributor.authorYeung, WSBen_US
dc.contributor.authorChiu, PCNen_US
dc.date.accessioned2011-12-21T08:59:50Z-
dc.date.available2011-12-21T08:59:50Z-
dc.date.issued2011en_US
dc.identifier.citationThe 44th Annual Meeting of the Society for the Study of Reproduction (SSR 2011), Portland, OR., 31 July-4 August 2011. In Abstracts of the 44th SSR, 2011, p. 167, abstract no. 711en_US
dc.identifier.urihttp://hdl.handle.net/10722/143942-
dc.descriptionConference Theme: Reproduction and the World’s Future-
dc.descriptionPoster Session B - Reproductive immunology: abstract no. 711-
dc.description.abstractMacrophages contribute to 20-30% of the overall population of leukocytes at the maternal-fetal interface. They are in close proximity with the trophoblast which take part in remodeling of placental tissue, clearing apoptotic cells and controlling trophoblast functions. Glycodelin isoforms comprise a group of secretory glycoproteins with four well defined glycoforms, each with a distinct glycosylation pattern. Glycodelin-A (GdA) is a uterine isoform with glycosylation-dependent immunomodulatory activities that contributes to feto-maternal defense. It is abundantly synthesized in the decidua during the first trimester of pregnancy. In the present study, we hypothesized that GdA may exert immunoregulatory activities on monocyte/macrophage and promotes their development into unique phenotypes for maintaining pregnancy. Monocytic cell line THP-1 and monocytes extracted from human female peripheral blood were used as study model. Macrophages were obtained from peripheral blood or by in vitro differentiation of the monocytes using granulocyte-macrophage colony-stimulating factor. Our results showed that GdA did not affect the proliferation and viability of monocytes and macrophages. GdA was demonstrated to up-regulate the interleukin (IL)-6 secretions in macrophage and monocyte as determined by ELISA and flow cytometry. Suppression of extracellular signal-regulated kinases (ERK) signaling abolished the stimulatory effect of GdA, indicating that GdA increased IL-6 production in monocytes/macrophages via the ERK activation. Interestingly, it has been reported that IL-6 is essential for the process of implantation, trophoblast invasion and embryo development. The inclusion of GdA also increased the indoleamine 2,3-dioxyenase (IDO) expression of macrophage, which subsequently inhibited the lymphocyte proliferation in a coculture study. IDO is a marker of decidual macrophage with immunosuppressive activity through depriving the access to tryptophan by immune cells. Taken together, the present data propose a novel role of GdA in modulating the cytokine profile and development of monocytes/macrophages in order to facilitate human pregnancy.-
dc.languageengen_US
dc.publisherSociety for the Study of Reproduction.-
dc.relation.ispartofAbstracts of the 44th Annual Meeting of the Society for the Study of Reproduction, SSR 2011en_US
dc.titleThe immunomodulatory properties of glycodelin-A on human monocyte and macrophageen_US
dc.typeConference_Paperen_US
dc.identifier.emailLam, EYF: h0600782@HKUSUA.hku.hken_US
dc.identifier.emailLee, CL: kcllee@hku.hken_US
dc.identifier.emailYeung, WSB: wsbyeung@hkucc.hku.hken_US
dc.identifier.emailChiu, PCN: pchiucn@hku.hk-
dc.identifier.authorityYeung, WSB=rp00331en_US
dc.identifier.authorityChiu, PCN=rp00424en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.hkuros197834en_US
dc.identifier.hkuros209436-
dc.identifier.spage167-
dc.identifier.epage167-
dc.publisher.placeUnited States-
dc.description.otherThe 44th Annual Meeting of the Society for the Study of Reproduction (SSR 2011), Portland, OR., 31 July-4 August 2011. In Abstracts of the 44th SSR, 2011, p. 167, abstract no. 711-

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