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Article: Biomarkers of oxidative damage in cigarette smokers: Which biomarkers might reflect acute versus chronic oxidative stress?

TitleBiomarkers of oxidative damage in cigarette smokers: Which biomarkers might reflect acute versus chronic oxidative stress?
Authors
KeywordsCholesterol oxidation products
Cigarette smoking
Free radicals
Hydroxyeicosatetraenoic acid products
Isoprostanes
Oxidative stress
Issue Date2011
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/freeradbiomed
Citation
Free Radical Biology And Medicine, 2011, v. 50 n. 12, p. 1787-1793 How to Cite?
AbstractCigarette smoking predisposes to the development of multiple diseases involving oxidative damage. We measured a range of oxidative damage biomarkers to understand which differ between smokers and nonsmokers and if the levels of these biomarkers change further during the act of smoking itself. Despite overnight abstinence from smoking, smokers had higher levels of plasma total and esterified F 2-isoprostanes, hydroxyeicosatetraenoic acid products (HETEs), F 4-neuroprostanes, 7-ketocholesterol, and 24- and 27-hydroxycholesterol. Levels of urinary F 2-isoprostanes, HETEs, and 8-hydroxy-2′-deoxyguanosine were also increased compared with age-matched nonsmokers. Several biomarkers (plasma free F 2-isoprostanes, allantoin, and 7β-hydroxycholesterol and urinary F 2-isoprostane metabolites) were not elevated. The smokers were then asked to smoke a cigarette; this acute smoking elevated plasma and urinary F 2- isoprostanes, plasma allantoin, and certain cholesterol oxidation products compared to presmoking levels, but not plasma HETEs or urinary 8-hydroxy-2′-deoxyguanosine. Smokers showed differences in plasma fatty acid composition. Our findings confirm that certain oxidative damage biomarkers are elevated in smokers even after a period of abstinence from smoking, whereas these plus some others are elevated after acute smoking. Thus, different biomarkers do not measure identical aspects of oxidative stress. © 2011 Elsevier Inc.
Persistent Identifierhttp://hdl.handle.net/10722/143939
ISSN
2015 Impact Factor: 5.784
2015 SCImago Journal Rankings: 2.468
ISI Accession Number ID
Funding AgencyGrant Number
National Medical Research CouncilNMRC/1157/2008
Funding Information:

We thank Dr. Ginger Milne and the Eicosanoid Core Laboratory, Vanderbilt University (Nashville, TN, USA), for providing the standards for F<INF>4</INF>-neuroprostane measurements. We are grateful to the National Medical Research Council (Grant NMRC/1157/2008) for their generous support of this study.

References

 

DC FieldValueLanguage
dc.contributor.authorSeet, RCSen_HK
dc.contributor.authorLee, CYJen_HK
dc.contributor.authorLoke, WMen_HK
dc.contributor.authorHuang, SHen_HK
dc.contributor.authorHuang, Hen_HK
dc.contributor.authorLooi, WFen_HK
dc.contributor.authorChew, ESen_HK
dc.contributor.authorQuek, AMLen_HK
dc.contributor.authorLim, ECHen_HK
dc.contributor.authorHalliwell, Ben_HK
dc.date.accessioned2011-12-21T08:59:41Z-
dc.date.available2011-12-21T08:59:41Z-
dc.date.issued2011en_HK
dc.identifier.citationFree Radical Biology And Medicine, 2011, v. 50 n. 12, p. 1787-1793en_HK
dc.identifier.issn0891-5849en_HK
dc.identifier.urihttp://hdl.handle.net/10722/143939-
dc.description.abstractCigarette smoking predisposes to the development of multiple diseases involving oxidative damage. We measured a range of oxidative damage biomarkers to understand which differ between smokers and nonsmokers and if the levels of these biomarkers change further during the act of smoking itself. Despite overnight abstinence from smoking, smokers had higher levels of plasma total and esterified F 2-isoprostanes, hydroxyeicosatetraenoic acid products (HETEs), F 4-neuroprostanes, 7-ketocholesterol, and 24- and 27-hydroxycholesterol. Levels of urinary F 2-isoprostanes, HETEs, and 8-hydroxy-2′-deoxyguanosine were also increased compared with age-matched nonsmokers. Several biomarkers (plasma free F 2-isoprostanes, allantoin, and 7β-hydroxycholesterol and urinary F 2-isoprostane metabolites) were not elevated. The smokers were then asked to smoke a cigarette; this acute smoking elevated plasma and urinary F 2- isoprostanes, plasma allantoin, and certain cholesterol oxidation products compared to presmoking levels, but not plasma HETEs or urinary 8-hydroxy-2′-deoxyguanosine. Smokers showed differences in plasma fatty acid composition. Our findings confirm that certain oxidative damage biomarkers are elevated in smokers even after a period of abstinence from smoking, whereas these plus some others are elevated after acute smoking. Thus, different biomarkers do not measure identical aspects of oxidative stress. © 2011 Elsevier Inc.en_HK
dc.languageengen_US
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/freeradbiomeden_HK
dc.relation.ispartofFree Radical Biology and Medicineen_HK
dc.subjectCholesterol oxidation productsen_HK
dc.subjectCigarette smokingen_HK
dc.subjectFree radicalsen_HK
dc.subjectHydroxyeicosatetraenoic acid productsen_HK
dc.subjectIsoprostanesen_HK
dc.subjectOxidative stressen_HK
dc.subject.meshAllantoin - blood-
dc.subject.meshF2-Isoprostanes - blood - metabolism - urine-
dc.subject.meshHydroxycholesterols - blood - metabolism-
dc.subject.meshHydroxyeicosatetraenoic Acids - blood - metabolism - urine-
dc.subject.meshSmoking - blood - metabolism - urine-
dc.titleBiomarkers of oxidative damage in cigarette smokers: Which biomarkers might reflect acute versus chronic oxidative stress?en_HK
dc.typeArticleen_HK
dc.identifier.emailLee, CYJ: jettylee@hku.hken_HK
dc.identifier.authorityLee, CYJ=rp01511en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.freeradbiomed.2011.03.019en_HK
dc.identifier.pmid21420490-
dc.identifier.scopuseid_2-s2.0-79956087559en_HK
dc.identifier.hkuros197985en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79956087559&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume50en_HK
dc.identifier.issue12en_HK
dc.identifier.spage1787en_HK
dc.identifier.epage1793en_HK
dc.identifier.eissn1873-4596-
dc.identifier.isiWOS:000291233200009-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridSeet, RCS=10045357300en_HK
dc.identifier.scopusauthoridLee, CYJ=13104265200en_HK
dc.identifier.scopusauthoridLoke, WM=14052177900en_HK
dc.identifier.scopusauthoridHuang, SH=8367750600en_HK
dc.identifier.scopusauthoridHuang, H=7405614576en_HK
dc.identifier.scopusauthoridLooi, WF=36523459100en_HK
dc.identifier.scopusauthoridChew, ES=50560920500en_HK
dc.identifier.scopusauthoridQuek, AML=13605538000en_HK
dc.identifier.scopusauthoridLim, ECH=35315865500en_HK
dc.identifier.scopusauthoridHalliwell, B=7101878919en_HK

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