Article: Establishment and characterization of human non-small cell lung cancer cell lines

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TitleEstablishment and characterization of human non-small cell lung cancer cell lines
AuthorsLi, J1
Yang, H1
Chen, L2
Li, Y1
Zhu, Y1
Dai, Y1
Chen, K1
Ai, J1
Zeng, T1
Mao, X1
Liu, L1
Li, X1
Guan, XY1 2
Issue Date2012
PublisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com/mmr/
CitationMolecular Medicine Reports, 2012, v. 5 n. 1, p. 114-117 [How to Cite?]
DOI: http://dx.doi.org/10.3892/mmr.2011.613
AbstractNon-small cell lung cancer (NSCLC), a highly malignant tumor, is common in China and is associated with a very poor 5-year survival rate. To better understand the cancer biology of this disease, we report here the establishment of three new NSCLC cell lines, SCC210011, SCC211441 and ACC212102, from the tumor tissue of three NSCLC patients. By histological analysis, we found that all three cell lines displayed the typical features of endothelial cancer cells. The population doubling times of SCC210011, SCC211441 and ACC212102 cells were 42, 38 and 25 h, respectively. Our cytogenetic studies indicated that these cell lines exhibit structural and numerical chromosomal abnormalities. Furthermore, the tumorigenicity in nude mice was confirmed, and H&E staining results revealed that they resembled the primary tissue. These newly established cell lines may serve as useful models for studying the molecular pathogenesis of NSCLC.
ISSN1791-2997
2011 Impact Factor: 0.418
2011 SCImago Journal Rankings: 0.041
DOIhttp://dx.doi.org/10.3892/mmr.2011.613
ISI Accession Number IDWOS:000297486700021
Funding AgencyGrant Number
National Natural Science Foundation of China30772475
30700820
30971606
Sun Yat-Sen University85000-3171311
Nation Key Sci-Tech Special Project of China2008ZX10002-022
Funding Information:

This study was supported in part by grants from the National Natural Science Foundation of China (30772475, 30700820 and 30971606), the Sun Yat-Sen University 'Hundred Talents Program' (85000-3171311), and the Nation Key Sci-Tech Special Project of China (2008ZX10002-022).

ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorLi, J
dc.contributor.authorYang, H
dc.contributor.authorChen, L
dc.contributor.authorLi, Y
dc.contributor.authorZhu, Y
dc.contributor.authorDai, Y
dc.contributor.authorChen, K
dc.contributor.authorAi, J
dc.contributor.authorZeng, T
dc.contributor.authorMao, X
dc.contributor.authorLiu, L
dc.contributor.authorLi, X
dc.contributor.authorGuan, XY
dc.date.accessioned2011-12-21T08:48:21Z
dc.date.available2011-12-21T08:48:21Z
dc.date.issued2012
dc.description.abstractNon-small cell lung cancer (NSCLC), a highly malignant tumor, is common in China and is associated with a very poor 5-year survival rate. To better understand the cancer biology of this disease, we report here the establishment of three new NSCLC cell lines, SCC210011, SCC211441 and ACC212102, from the tumor tissue of three NSCLC patients. By histological analysis, we found that all three cell lines displayed the typical features of endothelial cancer cells. The population doubling times of SCC210011, SCC211441 and ACC212102 cells were 42, 38 and 25 h, respectively. Our cytogenetic studies indicated that these cell lines exhibit structural and numerical chromosomal abnormalities. Furthermore, the tumorigenicity in nude mice was confirmed, and H&E staining results revealed that they resembled the primary tissue. These newly established cell lines may serve as useful models for studying the molecular pathogenesis of NSCLC.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationMolecular Medicine Reports, 2012, v. 5 n. 1, p. 114-117 [How to Cite?]
DOI: http://dx.doi.org/10.3892/mmr.2011.613
dc.identifier.doihttp://dx.doi.org/10.3892/mmr.2011.613
dc.identifier.epage117
dc.identifier.hkuros198001
dc.identifier.isiWOS:000297486700021
Funding AgencyGrant Number
National Natural Science Foundation of China30772475
30700820
30971606
Sun Yat-Sen University85000-3171311
Nation Key Sci-Tech Special Project of China2008ZX10002-022
Funding Information:

This study was supported in part by grants from the National Natural Science Foundation of China (30772475, 30700820 and 30971606), the Sun Yat-Sen University 'Hundred Talents Program' (85000-3171311), and the Nation Key Sci-Tech Special Project of China (2008ZX10002-022).

dc.identifier.issn1791-2997
2011 Impact Factor: 0.418
2011 SCImago Journal Rankings: 0.041
dc.identifier.issue1
dc.identifier.pmid21971914
dc.identifier.scopuseid_2-s2.0-84855238397
dc.identifier.spage114
dc.identifier.urihttp://hdl.handle.net/10722/143745
dc.identifier.volume5
dc.languageeng
dc.publisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com/mmr/
dc.publisher.placeGreece
dc.relation.ispartofMolecular Medicine Reports
dc.relation.referencesReferences in Scopus
dc.subject.meshAged
dc.subject.meshAnimals
dc.subject.meshCarcinoma, Non-Small-Cell Lung - genetics - pathology
dc.subject.meshChromosome Aberrations
dc.subject.meshHumans
dc.subject.meshKaryotyping
dc.subject.meshLung Neoplasms - genetics - pathology
dc.subject.meshMale
dc.subject.meshMice
dc.subject.meshMice, Nude
dc.subject.meshMiddle Aged
dc.subject.meshNeoplasm Transplantation
dc.subject.meshTransplantation, Heterologous
dc.subject.meshTumor Cells, Cultured
dc.titleEstablishment and characterization of human non-small cell lung cancer cell lines
dc.typeArticle
Author Affiliations
  1. Sun Yat-Sen University Cancer Center
  2. The University of Hong Kong