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Article: Establishment and characterization of human non-small cell lung cancer cell lines
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TitleEstablishment and characterization of human non-small cell lung cancer cell lines
 
AuthorsLi, J1
Yang, H1
Chen, L2
Li, Y1
Zhu, Y1
Dai, Y1
Chen, K1
Ai, J1
Zeng, T1
Mao, X1
Liu, L1
Li, X1
Guan, XY1 2
 
Issue Date2012
 
PublisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com/mmr/
 
CitationMolecular Medicine Reports, 2012, v. 5 n. 1, p. 114-117 [How to Cite?]
DOI: http://dx.doi.org/10.3892/mmr.2011.613
 
AbstractNon-small cell lung cancer (NSCLC), a highly malignant tumor, is common in China and is associated with a very poor 5-year survival rate. To better understand the cancer biology of this disease, we report here the establishment of three new NSCLC cell lines, SCC210011, SCC211441 and ACC212102, from the tumor tissue of three NSCLC patients. By histological analysis, we found that all three cell lines displayed the typical features of endothelial cancer cells. The population doubling times of SCC210011, SCC211441 and ACC212102 cells were 42, 38 and 25 h, respectively. Our cytogenetic studies indicated that these cell lines exhibit structural and numerical chromosomal abnormalities. Furthermore, the tumorigenicity in nude mice was confirmed, and H&E staining results revealed that they resembled the primary tissue. These newly established cell lines may serve as useful models for studying the molecular pathogenesis of NSCLC.
 
ISSN1791-2997
2013 Impact Factor: 1.484
 
DOIhttp://dx.doi.org/10.3892/mmr.2011.613
 
ISI Accession Number IDWOS:000297486700021
Funding AgencyGrant Number
National Natural Science Foundation of China30772475
30700820
30971606
Sun Yat-Sen University85000-3171311
Nation Key Sci-Tech Special Project of China2008ZX10002-022
Funding Information:

This study was supported in part by grants from the National Natural Science Foundation of China (30772475, 30700820 and 30971606), the Sun Yat-Sen University 'Hundred Talents Program' (85000-3171311), and the Nation Key Sci-Tech Special Project of China (2008ZX10002-022).

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorLi, J
 
dc.contributor.authorYang, H
 
dc.contributor.authorChen, L
 
dc.contributor.authorLi, Y
 
dc.contributor.authorZhu, Y
 
dc.contributor.authorDai, Y
 
dc.contributor.authorChen, K
 
dc.contributor.authorAi, J
 
dc.contributor.authorZeng, T
 
dc.contributor.authorMao, X
 
dc.contributor.authorLiu, L
 
dc.contributor.authorLi, X
 
dc.contributor.authorGuan, XY
 
dc.date.accessioned2011-12-21T08:48:21Z
 
dc.date.available2011-12-21T08:48:21Z
 
dc.date.issued2012
 
dc.description.abstractNon-small cell lung cancer (NSCLC), a highly malignant tumor, is common in China and is associated with a very poor 5-year survival rate. To better understand the cancer biology of this disease, we report here the establishment of three new NSCLC cell lines, SCC210011, SCC211441 and ACC212102, from the tumor tissue of three NSCLC patients. By histological analysis, we found that all three cell lines displayed the typical features of endothelial cancer cells. The population doubling times of SCC210011, SCC211441 and ACC212102 cells were 42, 38 and 25 h, respectively. Our cytogenetic studies indicated that these cell lines exhibit structural and numerical chromosomal abnormalities. Furthermore, the tumorigenicity in nude mice was confirmed, and H&E staining results revealed that they resembled the primary tissue. These newly established cell lines may serve as useful models for studying the molecular pathogenesis of NSCLC.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationMolecular Medicine Reports, 2012, v. 5 n. 1, p. 114-117 [How to Cite?]
DOI: http://dx.doi.org/10.3892/mmr.2011.613
 
dc.identifier.doihttp://dx.doi.org/10.3892/mmr.2011.613
 
dc.identifier.epage117
 
dc.identifier.hkuros198001
 
dc.identifier.isiWOS:000297486700021
Funding AgencyGrant Number
National Natural Science Foundation of China30772475
30700820
30971606
Sun Yat-Sen University85000-3171311
Nation Key Sci-Tech Special Project of China2008ZX10002-022
Funding Information:

This study was supported in part by grants from the National Natural Science Foundation of China (30772475, 30700820 and 30971606), the Sun Yat-Sen University 'Hundred Talents Program' (85000-3171311), and the Nation Key Sci-Tech Special Project of China (2008ZX10002-022).

 
dc.identifier.issn1791-2997
2013 Impact Factor: 1.484
 
dc.identifier.issue1
 
dc.identifier.pmid21971914
 
dc.identifier.scopuseid_2-s2.0-84855238397
 
dc.identifier.spage114
 
dc.identifier.urihttp://hdl.handle.net/10722/143745
 
dc.identifier.volume5
 
dc.languageeng
 
dc.publisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com/mmr/
 
dc.publisher.placeGreece
 
dc.relation.ispartofMolecular Medicine Reports
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAged
 
dc.subject.meshAnimals
 
dc.subject.meshCarcinoma, Non-Small-Cell Lung - genetics - pathology
 
dc.subject.meshChromosome Aberrations
 
dc.subject.meshHumans
 
dc.subject.meshKaryotyping
 
dc.subject.meshLung Neoplasms - genetics - pathology
 
dc.subject.meshMale
 
dc.subject.meshMice
 
dc.subject.meshMice, Nude
 
dc.subject.meshMiddle Aged
 
dc.subject.meshNeoplasm Transplantation
 
dc.subject.meshTransplantation, Heterologous
 
dc.subject.meshTumor Cells, Cultured
 
dc.titleEstablishment and characterization of human non-small cell lung cancer cell lines
 
dc.typeArticle
 
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Author Affiliations
  1. Sun Yat-Sen University Cancer Center
  2. The University of Hong Kong