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Article: A powerful and rapid approach to human genome scanning using small quantities of genomic DNA

TitleA powerful and rapid approach to human genome scanning using small quantities of genomic DNA
Authors
Issue Date2001
PublisherCambridge University Press. The Journal's web site is located at http://journals.cambridge.org/action/displayJournal?jid=GRH
Citation
Genetical Research, 2001, v. 77 n. 2, p. 129-134 How to Cite?
AbstractDense maps of short-tandem-repeat polymorphisms (STRPs) have allowed genome-wide searches for genes involved in a great variety of diseases with genetic influences, including common complex diseases. Generally for this purpose, marker sets with a 10 cM spacing are genotyped in hundreds of individuals. We have performed power simulations to estimate the maximum possible intermarker distance that still allows for sufficient power. In this paper we further report on modifications of previously published protocols, resulting in a powerful screening set containing 229 STRPs with an average spacing of 18·3 cM. A complete genome scan using our protocol requires only 80 multiplex PCR reactions which are all carried out using one set of conditions and which do not contain overlapping marker allele sizes. The multiplex PCR reactions are grouped by sets of chromosomes, which enables on-line statistical analysis of a set of chromosomes, as sets of chromosomes are being genotyped. A genome scan following this modified protocol can be performed using a maximum amount of 2.5 μg of genomic DNA per individual, isolated from either blood or from mouth swabs.
Persistent Identifierhttp://hdl.handle.net/10722/143707
ISSN
2015 Impact Factor: 0.743
2015 SCImago Journal Rankings: 0.520
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorBeekman, Men_HK
dc.contributor.authorLakenberg, Nen_HK
dc.contributor.authorCherny, SSen_HK
dc.contributor.authorDe Knijff, Pen_HK
dc.contributor.authorCornelis Kluft, Cen_HK
dc.contributor.authorVan Ommen, GJBen_HK
dc.contributor.authorVogler, GPen_HK
dc.contributor.authorFrants, RRen_HK
dc.contributor.authorBoomsma, DIen_HK
dc.contributor.authorEline Slagboom, Pen_HK
dc.date.accessioned2011-12-16T08:09:52Z-
dc.date.available2011-12-16T08:09:52Z-
dc.date.issued2001en_HK
dc.identifier.citationGenetical Research, 2001, v. 77 n. 2, p. 129-134en_HK
dc.identifier.issn0016-6723en_HK
dc.identifier.urihttp://hdl.handle.net/10722/143707-
dc.description.abstractDense maps of short-tandem-repeat polymorphisms (STRPs) have allowed genome-wide searches for genes involved in a great variety of diseases with genetic influences, including common complex diseases. Generally for this purpose, marker sets with a 10 cM spacing are genotyped in hundreds of individuals. We have performed power simulations to estimate the maximum possible intermarker distance that still allows for sufficient power. In this paper we further report on modifications of previously published protocols, resulting in a powerful screening set containing 229 STRPs with an average spacing of 18·3 cM. A complete genome scan using our protocol requires only 80 multiplex PCR reactions which are all carried out using one set of conditions and which do not contain overlapping marker allele sizes. The multiplex PCR reactions are grouped by sets of chromosomes, which enables on-line statistical analysis of a set of chromosomes, as sets of chromosomes are being genotyped. A genome scan following this modified protocol can be performed using a maximum amount of 2.5 μg of genomic DNA per individual, isolated from either blood or from mouth swabs.en_HK
dc.publisherCambridge University Press. The Journal's web site is located at http://journals.cambridge.org/action/displayJournal?jid=GRHen_HK
dc.relation.ispartofGenetical Researchen_HK
dc.titleA powerful and rapid approach to human genome scanning using small quantities of genomic DNAen_HK
dc.typeArticleen_HK
dc.identifier.emailCherny, SS: cherny@hku.hken_HK
dc.identifier.authorityCherny, SS=rp00232en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1017/S001667230100492Xen_HK
dc.identifier.pmid11355568-
dc.identifier.scopuseid_2-s2.0-17744377603en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-17744377603&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume77en_HK
dc.identifier.issue2en_HK
dc.identifier.spage129en_HK
dc.identifier.epage134en_HK
dc.identifier.isiWOS:000168515700002-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridBeekman, M=7003537686en_HK
dc.identifier.scopusauthoridLakenberg, N=7801569879en_HK
dc.identifier.scopusauthoridCherny, SS=7004670001en_HK
dc.identifier.scopusauthoridDe Knijff, P=7005969770en_HK
dc.identifier.scopusauthoridCornelis Kluft, C=18834225000en_HK
dc.identifier.scopusauthoridVan Ommen, GJB=35411955100en_HK
dc.identifier.scopusauthoridVogler, GP=7007004436en_HK
dc.identifier.scopusauthoridFrants, RR=35393585000en_HK
dc.identifier.scopusauthoridBoomsma, DI=35378012400en_HK
dc.identifier.scopusauthoridEline Slagboom, P=6506005024en_HK

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