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Article: In vivo production of mineralised tissue pieces for clinical use: A qualitative pilot study using human dental pulp cell

TitleIn vivo production of mineralised tissue pieces for clinical use: A qualitative pilot study using human dental pulp cell
Authors
Keywordsanimal testing
human dental pulp cell
in vivo study
in vivo transplantation
osteogenesis
tissue engineering
tissue engineering methods
Issue Date2011
PublisherChurchill Livingstone. The Journal's web site is located at http://www.elsevier.com/locate/ijom
Citation
International Journal Of Oral And Maxillofacial Surgery, 2011, v. 40 n. 6, p. 612-620 How to Cite?
Abstract
Numerous previous studies have investigated the production of mineralised tissues by transplanting human dental pulp cells with calcium based scaffolds. The potential of alternative setups remains largely uninvestigated, therefore in this study, human dental pulp cells were encapsulated into non-calcium based biomaterial - self-assembling peptide nano-fibre hydrogel. The cell-gel constructs were cultured in full medium for 2 weeks. Then they were cultured in full medium supplemented with β-glycerophosphate, dexamethasone and l-ascorbic acid for 2 more weeks. These cell-gel constructs and plain-gel constructs (with no cells) were transplanted subcutaneously into five nude mice. The gel constructs were retrieved 4 weeks after surgery. The plain-gel constructs were all completely resorbed with no new tissue formation. The cell-gel constructs were transformed into tissue pieces that were mineralised and contained blood capillaries. Immunohistochemistry analysis confirmed the expression of multiple bone markers (osteopontin, osteocalcin, osteonectin and parathyroid hormone receptor) in these tissue pieces. Computerised analysis of the contact radiographs gave the mean radio-opaque area percentage as 78% (N = 5, P < 0.001 compared with the 0% of the control). The results demonstrate good prospects for using human dental pulp cell plus self-assembling peptide nano-fibre hydrogel to produce mineralised tissue pieces for clinical use.
Persistent Identifierhttp://hdl.handle.net/10722/143482
ISSN
2013 Impact Factor: 1.359
2013 SCImago Journal Rankings: 0.953
ISI Accession Number ID
Funding AgencyGrant Number
20004794.22311.08003.400.01
Funding Information:

Donation A/C: 20004794.22311.08003.400.01. Clinical Trials to Induce Bone Formation.

References

 

DC FieldValueLanguage
dc.contributor.authorChan, Ben_HK
dc.contributor.authorWong, RWKen_HK
dc.contributor.authorRabie, Ben_HK
dc.date.accessioned2011-12-06T03:41:53Z-
dc.date.available2011-12-06T03:41:53Z-
dc.date.issued2011en_HK
dc.identifier.citationInternational Journal Of Oral And Maxillofacial Surgery, 2011, v. 40 n. 6, p. 612-620en_HK
dc.identifier.issn0901-5027en_HK
dc.identifier.urihttp://hdl.handle.net/10722/143482-
dc.description.abstractNumerous previous studies have investigated the production of mineralised tissues by transplanting human dental pulp cells with calcium based scaffolds. The potential of alternative setups remains largely uninvestigated, therefore in this study, human dental pulp cells were encapsulated into non-calcium based biomaterial - self-assembling peptide nano-fibre hydrogel. The cell-gel constructs were cultured in full medium for 2 weeks. Then they were cultured in full medium supplemented with β-glycerophosphate, dexamethasone and l-ascorbic acid for 2 more weeks. These cell-gel constructs and plain-gel constructs (with no cells) were transplanted subcutaneously into five nude mice. The gel constructs were retrieved 4 weeks after surgery. The plain-gel constructs were all completely resorbed with no new tissue formation. The cell-gel constructs were transformed into tissue pieces that were mineralised and contained blood capillaries. Immunohistochemistry analysis confirmed the expression of multiple bone markers (osteopontin, osteocalcin, osteonectin and parathyroid hormone receptor) in these tissue pieces. Computerised analysis of the contact radiographs gave the mean radio-opaque area percentage as 78% (N = 5, P < 0.001 compared with the 0% of the control). The results demonstrate good prospects for using human dental pulp cell plus self-assembling peptide nano-fibre hydrogel to produce mineralised tissue pieces for clinical use.en_HK
dc.languageeng-
dc.publisherChurchill Livingstone. The Journal's web site is located at http://www.elsevier.com/locate/ijomen_HK
dc.relation.ispartofInternational Journal of Oral and Maxillofacial Surgeryen_HK
dc.subjectanimal testingen_HK
dc.subjecthuman dental pulp cellen_HK
dc.subjectin vivo studyen_HK
dc.subjectin vivo transplantationen_HK
dc.subjectosteogenesisen_HK
dc.subjecttissue engineeringen_HK
dc.subjecttissue engineering methodsen_HK
dc.subject.meshCalcification, Physiologic - physiology-
dc.subject.meshDental Pulp - cytology - drug effects-
dc.subject.meshStem Cells - drug effects - physiology-
dc.subject.meshTissue Engineering - methods-
dc.subject.meshTissue Scaffolds - chemistry-
dc.titleIn vivo production of mineralised tissue pieces for clinical use: A qualitative pilot study using human dental pulp cellen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0901-5027&volume=40&issue=6&spage=612&epage=620&date=2011&atitle=In+vivo+production+of+mineralised+tissue+pieces+for+clinical+use:+a+qualitative+pilot+study+using+human+dental+pulp+cell-
dc.identifier.emailChan, B: bpchan@hkucc.hku.hken_HK
dc.identifier.emailRabie, B: rabie@hku.hken_HK
dc.identifier.authorityChan, B=rp00087en_HK
dc.identifier.authorityRabie, B=rp00029en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.ijom.2011.01.008en_HK
dc.identifier.pmid21353764en_HK
dc.identifier.scopuseid_2-s2.0-79956270840en_HK
dc.identifier.hkuros185524-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79956270840&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume40en_HK
dc.identifier.issue6en_HK
dc.identifier.spage612en_HK
dc.identifier.epage620en_HK
dc.identifier.eissn1399-0020-
dc.identifier.isiWOS:000291771800010-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridChan, B=7201530390en_HK
dc.identifier.scopusauthoridWong, RWK=36642905800en_HK
dc.identifier.scopusauthoridRabie, B=7007172734en_HK

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