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Article: In vivo production of mineralised tissue pieces for clinical use: A qualitative pilot study using human dental pulp cell
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TitleIn vivo production of mineralised tissue pieces for clinical use: A qualitative pilot study using human dental pulp cell
 
AuthorsChan, B1
Wong, RWK1
Rabie, B1
 
Keywordsanimal testing
human dental pulp cell
in vivo study
in vivo transplantation
osteogenesis
tissue engineering
tissue engineering methods
 
Issue Date2011
 
PublisherChurchill Livingstone. The Journal's web site is located at http://www.elsevier.com/locate/ijom
 
CitationInternational Journal Of Oral And Maxillofacial Surgery, 2011, v. 40 n. 6, p. 612-620 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.ijom.2011.01.008
 
AbstractNumerous previous studies have investigated the production of mineralised tissues by transplanting human dental pulp cells with calcium based scaffolds. The potential of alternative setups remains largely uninvestigated, therefore in this study, human dental pulp cells were encapsulated into non-calcium based biomaterial - self-assembling peptide nano-fibre hydrogel. The cell-gel constructs were cultured in full medium for 2 weeks. Then they were cultured in full medium supplemented with β-glycerophosphate, dexamethasone and l-ascorbic acid for 2 more weeks. These cell-gel constructs and plain-gel constructs (with no cells) were transplanted subcutaneously into five nude mice. The gel constructs were retrieved 4 weeks after surgery. The plain-gel constructs were all completely resorbed with no new tissue formation. The cell-gel constructs were transformed into tissue pieces that were mineralised and contained blood capillaries. Immunohistochemistry analysis confirmed the expression of multiple bone markers (osteopontin, osteocalcin, osteonectin and parathyroid hormone receptor) in these tissue pieces. Computerised analysis of the contact radiographs gave the mean radio-opaque area percentage as 78% (N = 5, P < 0.001 compared with the 0% of the control). The results demonstrate good prospects for using human dental pulp cell plus self-assembling peptide nano-fibre hydrogel to produce mineralised tissue pieces for clinical use.
 
ISSN0901-5027
2013 Impact Factor: 1.359
2013 SCImago Journal Rankings: 0.953
 
DOIhttp://dx.doi.org/10.1016/j.ijom.2011.01.008
 
ISI Accession Number IDWOS:000291771800010
Funding AgencyGrant Number
20004794.22311.08003.400.01
Funding Information:

Donation A/C: 20004794.22311.08003.400.01. Clinical Trials to Induce Bone Formation.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorChan, B
 
dc.contributor.authorWong, RWK
 
dc.contributor.authorRabie, B
 
dc.date.accessioned2011-12-06T03:41:53Z
 
dc.date.available2011-12-06T03:41:53Z
 
dc.date.issued2011
 
dc.description.abstractNumerous previous studies have investigated the production of mineralised tissues by transplanting human dental pulp cells with calcium based scaffolds. The potential of alternative setups remains largely uninvestigated, therefore in this study, human dental pulp cells were encapsulated into non-calcium based biomaterial - self-assembling peptide nano-fibre hydrogel. The cell-gel constructs were cultured in full medium for 2 weeks. Then they were cultured in full medium supplemented with β-glycerophosphate, dexamethasone and l-ascorbic acid for 2 more weeks. These cell-gel constructs and plain-gel constructs (with no cells) were transplanted subcutaneously into five nude mice. The gel constructs were retrieved 4 weeks after surgery. The plain-gel constructs were all completely resorbed with no new tissue formation. The cell-gel constructs were transformed into tissue pieces that were mineralised and contained blood capillaries. Immunohistochemistry analysis confirmed the expression of multiple bone markers (osteopontin, osteocalcin, osteonectin and parathyroid hormone receptor) in these tissue pieces. Computerised analysis of the contact radiographs gave the mean radio-opaque area percentage as 78% (N = 5, P < 0.001 compared with the 0% of the control). The results demonstrate good prospects for using human dental pulp cell plus self-assembling peptide nano-fibre hydrogel to produce mineralised tissue pieces for clinical use.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationInternational Journal Of Oral And Maxillofacial Surgery, 2011, v. 40 n. 6, p. 612-620 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.ijom.2011.01.008
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.ijom.2011.01.008
 
dc.identifier.eissn1399-0020
 
dc.identifier.epage620
 
dc.identifier.hkuros185524
 
dc.identifier.isiWOS:000291771800010
Funding AgencyGrant Number
20004794.22311.08003.400.01
Funding Information:

Donation A/C: 20004794.22311.08003.400.01. Clinical Trials to Induce Bone Formation.

 
dc.identifier.issn0901-5027
2013 Impact Factor: 1.359
2013 SCImago Journal Rankings: 0.953
 
dc.identifier.issue6
 
dc.identifier.openurl
 
dc.identifier.pmid21353764
 
dc.identifier.scopuseid_2-s2.0-79956270840
 
dc.identifier.spage612
 
dc.identifier.urihttp://hdl.handle.net/10722/143482
 
dc.identifier.volume40
 
dc.languageeng
 
dc.publisherChurchill Livingstone. The Journal's web site is located at http://www.elsevier.com/locate/ijom
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofInternational Journal of Oral and Maxillofacial Surgery
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshCalcification, Physiologic - physiology
 
dc.subject.meshDental Pulp - cytology - drug effects
 
dc.subject.meshStem Cells - drug effects - physiology
 
dc.subject.meshTissue Engineering - methods
 
dc.subject.meshTissue Scaffolds - chemistry
 
dc.subjectanimal testing
 
dc.subjecthuman dental pulp cell
 
dc.subjectin vivo study
 
dc.subjectin vivo transplantation
 
dc.subjectosteogenesis
 
dc.subjecttissue engineering
 
dc.subjecttissue engineering methods
 
dc.titleIn vivo production of mineralised tissue pieces for clinical use: A qualitative pilot study using human dental pulp cell
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong