Article: In vivo production of mineralised tissue pieces for clinical use: A qualitative pilot study using human dental pulp cell

File Download Links for fulltext
(May Require Subscription)
Supplementary
  • Basic View
  • Metadata View
  • XML View
TitleIn vivo production of mineralised tissue pieces for clinical use: A qualitative pilot study using human dental pulp cell
AuthorsChan, B1
Wong, RWK1
Rabie, B1
Keywordsanimal testing
human dental pulp cell
in vivo study
in vivo transplantation
osteogenesis
tissue engineering
tissue engineering methods
Issue Date2011
PublisherChurchill Livingstone. The Journal's web site is located at http://www.elsevier.com/locate/ijom
CitationInternational Journal Of Oral And Maxillofacial Surgery, 2011, v. 40 n. 6, p. 612-620 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.ijom.2011.01.008
AbstractNumerous previous studies have investigated the production of mineralised tissues by transplanting human dental pulp cells with calcium based scaffolds. The potential of alternative setups remains largely uninvestigated, therefore in this study, human dental pulp cells were encapsulated into non-calcium based biomaterial - self-assembling peptide nano-fibre hydrogel. The cell-gel constructs were cultured in full medium for 2 weeks. Then they were cultured in full medium supplemented with β-glycerophosphate, dexamethasone and l-ascorbic acid for 2 more weeks. These cell-gel constructs and plain-gel constructs (with no cells) were transplanted subcutaneously into five nude mice. The gel constructs were retrieved 4 weeks after surgery. The plain-gel constructs were all completely resorbed with no new tissue formation. The cell-gel constructs were transformed into tissue pieces that were mineralised and contained blood capillaries. Immunohistochemistry analysis confirmed the expression of multiple bone markers (osteopontin, osteocalcin, osteonectin and parathyroid hormone receptor) in these tissue pieces. Computerised analysis of the contact radiographs gave the mean radio-opaque area percentage as 78% (N = 5, P < 0.001 compared with the 0% of the control). The results demonstrate good prospects for using human dental pulp cell plus self-assembling peptide nano-fibre hydrogel to produce mineralised tissue pieces for clinical use.
ISSN0901-5027
2011 Impact Factor: 1.506
2011 SCImago Journal Rankings: 0.089
DOIhttp://dx.doi.org/10.1016/j.ijom.2011.01.008
ISI Accession Number IDWOS:000291771800010
Funding AgencyGrant Number
20004794.22311.08003.400.01
Funding Information:

Donation A/C: 20004794.22311.08003.400.01. Clinical Trials to Induce Bone Formation.

ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorChan, B
dc.contributor.authorWong, RWK
dc.contributor.authorRabie, B
dc.date.accessioned2011-12-06T03:41:53Z
dc.date.available2011-12-06T03:41:53Z
dc.date.issued2011
dc.description.abstractNumerous previous studies have investigated the production of mineralised tissues by transplanting human dental pulp cells with calcium based scaffolds. The potential of alternative setups remains largely uninvestigated, therefore in this study, human dental pulp cells were encapsulated into non-calcium based biomaterial - self-assembling peptide nano-fibre hydrogel. The cell-gel constructs were cultured in full medium for 2 weeks. Then they were cultured in full medium supplemented with β-glycerophosphate, dexamethasone and l-ascorbic acid for 2 more weeks. These cell-gel constructs and plain-gel constructs (with no cells) were transplanted subcutaneously into five nude mice. The gel constructs were retrieved 4 weeks after surgery. The plain-gel constructs were all completely resorbed with no new tissue formation. The cell-gel constructs were transformed into tissue pieces that were mineralised and contained blood capillaries. Immunohistochemistry analysis confirmed the expression of multiple bone markers (osteopontin, osteocalcin, osteonectin and parathyroid hormone receptor) in these tissue pieces. Computerised analysis of the contact radiographs gave the mean radio-opaque area percentage as 78% (N = 5, P < 0.001 compared with the 0% of the control). The results demonstrate good prospects for using human dental pulp cell plus self-assembling peptide nano-fibre hydrogel to produce mineralised tissue pieces for clinical use.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationInternational Journal Of Oral And Maxillofacial Surgery, 2011, v. 40 n. 6, p. 612-620 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.ijom.2011.01.008
dc.identifier.doihttp://dx.doi.org/10.1016/j.ijom.2011.01.008
dc.identifier.epage620
dc.identifier.hkuros185524
dc.identifier.isiWOS:000291771800010
Funding AgencyGrant Number
20004794.22311.08003.400.01
Funding Information:

Donation A/C: 20004794.22311.08003.400.01. Clinical Trials to Induce Bone Formation.

dc.identifier.issn0901-5027
2011 Impact Factor: 1.506
2011 SCImago Journal Rankings: 0.089
dc.identifier.issue6
dc.identifier.openurl
dc.identifier.pmid21353764
dc.identifier.scopuseid_2-s2.0-79956270840
dc.identifier.spage612
dc.identifier.urihttp://hdl.handle.net/10722/143482
dc.identifier.volume40
dc.languageeng
dc.publisherChurchill Livingstone. The Journal's web site is located at http://www.elsevier.com/locate/ijom
dc.publisher.placeUnited Kingdom
dc.relation.ispartofInternational Journal of Oral and Maxillofacial Surgery
dc.relation.referencesReferences in Scopus
dc.subject.meshCalcification, Physiologic - physiology
dc.subject.meshDental Pulp - cytology - drug effects
dc.subject.meshStem Cells - drug effects - physiology
dc.subject.meshTissue Engineering - methods
dc.subject.meshTissue Scaffolds - chemistry
dc.subjectanimal testing
dc.subjecthuman dental pulp cell
dc.subjectin vivo study
dc.subjectin vivo transplantation
dc.subjectosteogenesis
dc.subjecttissue engineering
dc.subjecttissue engineering methods
dc.titleIn vivo production of mineralised tissue pieces for clinical use: A qualitative pilot study using human dental pulp cell
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong