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Article: Resequencing microarray for detection of human adenoviruses in patients with conjunctivitis

TitleResequencing microarray for detection of human adenoviruses in patients with conjunctivitis
Authors
KeywordsConjunctivitis
Culture
Human adenovirus
Patients
PCR
Resequencing microarray
Issue Date2010
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jcv
Citation
Journal Of Clinical Virology, 2010, v. 47 n. 3, p. 282-285 How to Cite?
AbstractBackground: Although high-density resequencing microarray is useful for detection and tracking the evolution of viruses associated with respiratory tract infections, no report on using this technology for the detection of viruses in patients with conjunctivitis is available. Objectives: To test if high-density resequencing microarray can be applied to detection of viruses in conjunctival swabs for patients with conjunctivitis. Study design: In this prospective proof-of-concept study, every 4 or 5 bacterial culture-negative conjunctival swab samples were pooled and subject to viral detection using TessArray™ Resequencing Pathogen Microarrays-Flu 3.1 (RPM-Flu-3.1). Results were compared with human adenovirus (HAdV) hexon gene PCR sequencing and viral culture. Results: Thirty-two of the 38 conjunctival swab samples were bacterial culture-negative. Four of the 7 pooled samples were positive for HAdV using RPM-Flu-3.1. Hexon gene PCR sequencing on the 38 original individual samples showed that 3 and 4 samples contained HAdVs species D and B respectively. All the 6 samples that were positive for hexon gene PCR but negative for bacterial culture were also positive by the resequencing microarray. Viral culture was positive for HAdV type 3 in 1 sample, which was also positive by PCR and resequencing microarray. Conclusions: Resequencing microarray is as sensitive as PCR for detection of HAdV in conjunctival swabs. Unlike viral culture and hexon gene PCR sequencing, resequencing microarray was not able to differentiate the type and species of HAdV. Development of microarrays for conjunctivitis can be performed for rapid diagnosis of the viral cause of conjunctivitis. © 2009 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/143464
ISSN
2015 Impact Factor: 2.647
2015 SCImago Journal Rankings: 1.503
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Special Administrative Region of China
University Development Fund
University of Hong Kong
Shaw Foundation
Funding Information:

This work is partly supported by the Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for Department of Health of the Hong Kong Special Administrative Region of China; Research Grant Council Grant; University Development Fund, the Committee for Research and Conference Grant, and Outstanding Young Researcher Award, The University of Hong Kong; and the Shaw Foundation.

References

 

DC FieldValueLanguage
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorLau, SKPen_HK
dc.contributor.authorChoi, GKYen_HK
dc.contributor.authorFung, HTen_HK
dc.contributor.authorShek, KCen_HK
dc.contributor.authorMiao, Jen_HK
dc.contributor.authorChan, BYLen_HK
dc.contributor.authorNg, KHLen_HK
dc.contributor.authorNgan, AHYen_HK
dc.contributor.authorEllisBehnke, Ren_HK
dc.contributor.authorQue, TLen_HK
dc.contributor.authorKam, CWen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2011-12-01T06:13:58Z-
dc.date.available2011-12-01T06:13:58Z-
dc.date.issued2010en_HK
dc.identifier.citationJournal Of Clinical Virology, 2010, v. 47 n. 3, p. 282-285en_HK
dc.identifier.issn1386-6532en_HK
dc.identifier.urihttp://hdl.handle.net/10722/143464-
dc.description.abstractBackground: Although high-density resequencing microarray is useful for detection and tracking the evolution of viruses associated with respiratory tract infections, no report on using this technology for the detection of viruses in patients with conjunctivitis is available. Objectives: To test if high-density resequencing microarray can be applied to detection of viruses in conjunctival swabs for patients with conjunctivitis. Study design: In this prospective proof-of-concept study, every 4 or 5 bacterial culture-negative conjunctival swab samples were pooled and subject to viral detection using TessArray™ Resequencing Pathogen Microarrays-Flu 3.1 (RPM-Flu-3.1). Results were compared with human adenovirus (HAdV) hexon gene PCR sequencing and viral culture. Results: Thirty-two of the 38 conjunctival swab samples were bacterial culture-negative. Four of the 7 pooled samples were positive for HAdV using RPM-Flu-3.1. Hexon gene PCR sequencing on the 38 original individual samples showed that 3 and 4 samples contained HAdVs species D and B respectively. All the 6 samples that were positive for hexon gene PCR but negative for bacterial culture were also positive by the resequencing microarray. Viral culture was positive for HAdV type 3 in 1 sample, which was also positive by PCR and resequencing microarray. Conclusions: Resequencing microarray is as sensitive as PCR for detection of HAdV in conjunctival swabs. Unlike viral culture and hexon gene PCR sequencing, resequencing microarray was not able to differentiate the type and species of HAdV. Development of microarrays for conjunctivitis can be performed for rapid diagnosis of the viral cause of conjunctivitis. © 2009 Elsevier B.V. All rights reserved.en_HK
dc.languageeng-
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jcven_HK
dc.relation.ispartofJournal of Clinical Virologyen_HK
dc.subjectConjunctivitisen_HK
dc.subjectCultureen_HK
dc.subjectHuman adenovirusen_HK
dc.subjectPatientsen_HK
dc.subjectPCRen_HK
dc.subjectResequencing microarrayen_HK
dc.subject.meshAdenovirus Infections, Human - diagnosis - virology-
dc.subject.meshAdenoviruses, Human - isolation and purification-
dc.subject.meshConjunctivitis - virology-
dc.subject.meshMicroarray Analysis - methods-
dc.subject.meshSensitivity and Specificity-
dc.titleResequencing microarray for detection of human adenoviruses in patients with conjunctivitisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1386-6532&volume=47&issue=3&spage=282&epage=285&date=2010&atitle=Resequencing+microarray+for+detection+of+human+adenoviruses+in+patients+with+conjunctivitis-
dc.identifier.emailWoo, PCY: pcywoo@hkucc.hku.hken_HK
dc.identifier.emailLau, SKP: skplau@hkucc.hku.hken_HK
dc.identifier.emailEllisBehnke, R: rutledg@mit.eduen_HK
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityLau, SKP=rp00486en_HK
dc.identifier.authorityEllisBehnke, R=rp00252en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jcv.2009.12.014en_HK
dc.identifier.pmid20071220-
dc.identifier.scopuseid_2-s2.0-76449116953en_HK
dc.identifier.hkuros171969-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-76449116953&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume47en_HK
dc.identifier.issue3en_HK
dc.identifier.spage282en_HK
dc.identifier.epage285en_HK
dc.identifier.isiWOS:000274862700018-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridLau, SKP=7401596211en_HK
dc.identifier.scopusauthoridChoi, GKY=35423450200en_HK
dc.identifier.scopusauthoridFung, HT=7102805572en_HK
dc.identifier.scopusauthoridShek, KC=8061289800en_HK
dc.identifier.scopusauthoridMiao, J=36025802100en_HK
dc.identifier.scopusauthoridChan, BYL=7201530670en_HK
dc.identifier.scopusauthoridNg, KHL=27467782600en_HK
dc.identifier.scopusauthoridNgan, AHY=14037517900en_HK
dc.identifier.scopusauthoridEllisBehnke, R=8548055200en_HK
dc.identifier.scopusauthoridQue, TL=7003786628en_HK
dc.identifier.scopusauthoridKam, CW=7102416674en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK
dc.identifier.citeulike6555465-

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