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Article: Progress towards cloning the cystic fibrosis gene.

TitleProgress towards cloning the cystic fibrosis gene.
Authors
Issue Date1988
PublisherThe Royal Society. The Journal's web site is located at http://www.pubs.royalsoc.ac.uk/index.cfm?page=1085
Citation
Philosophical Transactions Of The Royal Society Of London. Series B: Biological Sciences, 1988, v. 319 n. 1194, p. 263-273 How to Cite?
AbstractGenetic linkage analysis with polymorphic DNA markers (restriction fragment length polymorphisms: RFLPS) has allowed the assignment of the cystic fibrosis (CF) locus to the long arm of chromosome 7, within the region of band q31. Two of these markers, MET and D7S8, are tightly linked to the disease locus. Although recent data suggest that they are located on opposite sides of CF, the two can be separated by as much as 5 centimorgans. To obtain a better description of the CF locus and, eventually, to identify the affected gene, additional DNA markers are required to connect MET and D7S8, physically. We have screened the flow-sorted chromosome-7-specific library and thus far isolated 28 new probes from the 7q31 region by DNA hybridization analysis that uses a series of somatic cell hybrids containing various portions of human chromosome 7. Together with the previously identified markers, MET, D7S8, D7S13 and D7S16, these new markers should provide a fine genetic and physical map for the chromosomal region surrounding CF. DNA segments can then be sequentially cloned by chromosome walking from points closest to the CF locus and examined for genes that are preferentially expressed in tissues known to be affected in the disease.
Persistent Identifierhttp://hdl.handle.net/10722/143457
ISSN
2015 Impact Factor: 5.847
2015 SCImago Journal Rankings: 2.659
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTsui, LCen_HK
dc.contributor.authorRommens, JMen_HK
dc.contributor.authorBurns, Jen_HK
dc.contributor.authorZengerling, Sen_HK
dc.contributor.authorRiordan, JRen_HK
dc.contributor.authorCarlock, LRen_HK
dc.contributor.authorGrzeschik, KHen_HK
dc.contributor.authorBuchwald, Men_HK
dc.date.accessioned2011-11-25T09:22:27Z-
dc.date.available2011-11-25T09:22:27Z-
dc.date.issued1988en_HK
dc.identifier.citationPhilosophical Transactions Of The Royal Society Of London. Series B: Biological Sciences, 1988, v. 319 n. 1194, p. 263-273en_HK
dc.identifier.issn0962-8436en_HK
dc.identifier.urihttp://hdl.handle.net/10722/143457-
dc.description.abstractGenetic linkage analysis with polymorphic DNA markers (restriction fragment length polymorphisms: RFLPS) has allowed the assignment of the cystic fibrosis (CF) locus to the long arm of chromosome 7, within the region of band q31. Two of these markers, MET and D7S8, are tightly linked to the disease locus. Although recent data suggest that they are located on opposite sides of CF, the two can be separated by as much as 5 centimorgans. To obtain a better description of the CF locus and, eventually, to identify the affected gene, additional DNA markers are required to connect MET and D7S8, physically. We have screened the flow-sorted chromosome-7-specific library and thus far isolated 28 new probes from the 7q31 region by DNA hybridization analysis that uses a series of somatic cell hybrids containing various portions of human chromosome 7. Together with the previously identified markers, MET, D7S8, D7S13 and D7S16, these new markers should provide a fine genetic and physical map for the chromosomal region surrounding CF. DNA segments can then be sequentially cloned by chromosome walking from points closest to the CF locus and examined for genes that are preferentially expressed in tissues known to be affected in the disease.en_HK
dc.languageeng-
dc.publisherThe Royal Society. The Journal's web site is located at http://www.pubs.royalsoc.ac.uk/index.cfm?page=1085en_HK
dc.relation.ispartofPhilosophical transactions of the Royal Society of London. Series B: Biological sciencesen_HK
dc.subject.meshChromosomes, Human, Pair 7-
dc.subject.meshCloning, Molecular-
dc.subject.meshCystic Fibrosis - genetics-
dc.subject.meshGenes-
dc.subject.meshHybrid Cells - cytology-
dc.titleProgress towards cloning the cystic fibrosis gene.en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0962-8436&volume=319&issue=1194&spage=263&epage=273&date=1988&atitle=Progress+towards+cloning+the+cystic+fibrosis+gene-
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1098/rstb.1988.0048-
dc.identifier.pmid2900520-
dc.identifier.scopuseid_2-s2.0-0024287997en_HK
dc.identifier.volume319en_HK
dc.identifier.issue1194en_HK
dc.identifier.spage263en_HK
dc.identifier.epage273en_HK
dc.identifier.isiWOS:A1988N885200005-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK
dc.identifier.scopusauthoridRommens, JM=7006884140en_HK
dc.identifier.scopusauthoridBurns, J=7403680971en_HK
dc.identifier.scopusauthoridZengerling, S=6507625753en_HK
dc.identifier.scopusauthoridRiordan, JR=7202229758en_HK
dc.identifier.scopusauthoridCarlock, LR=6603727620en_HK
dc.identifier.scopusauthoridGrzeschik, KH=7004321252en_HK
dc.identifier.scopusauthoridBuchwald, M=7006759922en_HK

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