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Article: High-resolution FISH analysis.

TitleHigh-resolution FISH analysis.
Authors
Issue Date2005
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.does.org/masterli/cphg.html
Citation
Current Protocols In Human Genetics / Editorial Board, Jonathan L. Haines ... [Et Al.], 2005, v. Chapter 4, p. Unit 4.5 How to Cite?
AbstractMap order, orientation, and gap or overlap distance of closely linked DNA probes may be determined using fluorescent hybridization to decondensed DNA. The linear arrangement of released chromatin fibers not only simplifies the task of gene ordering, but also provides higher resolution with probes separated by greater distances than can be achieved in FISH with intact interphase nuclei. The Basic Protocol 1 of this unit describes an alkaline lysis procedure for generating free chromatin from cultured cells for FISH analysis. A support protocol describes an empirical approach to optimize conditions for preparation of free chromatin. An Alternate Protocol 1 provides a method for producing free chromatin from cultured lymphocytes with drug treatment. The Basic Protocol 2, high-resolution FISH mapping with free chromatin, is a modification of the method used for FISH mapping of interphase nuclei.
Persistent Identifierhttp://hdl.handle.net/10722/143351
ISSN
2015 SCImago Journal Rankings: 1.834

 

DC FieldValueLanguage
dc.contributor.authorHeng, HHen_HK
dc.contributor.authorWindle, Ben_HK
dc.contributor.authorTsui, LCen_HK
dc.date.accessioned2011-11-24T04:13:57Z-
dc.date.available2011-11-24T04:13:57Z-
dc.date.issued2005en_HK
dc.identifier.citationCurrent Protocols In Human Genetics / Editorial Board, Jonathan L. Haines ... [Et Al.], 2005, v. Chapter 4, p. Unit 4.5en_HK
dc.identifier.issn1934-8258en_HK
dc.identifier.urihttp://hdl.handle.net/10722/143351-
dc.description.abstractMap order, orientation, and gap or overlap distance of closely linked DNA probes may be determined using fluorescent hybridization to decondensed DNA. The linear arrangement of released chromatin fibers not only simplifies the task of gene ordering, but also provides higher resolution with probes separated by greater distances than can be achieved in FISH with intact interphase nuclei. The Basic Protocol 1 of this unit describes an alkaline lysis procedure for generating free chromatin from cultured cells for FISH analysis. A support protocol describes an empirical approach to optimize conditions for preparation of free chromatin. An Alternate Protocol 1 provides a method for producing free chromatin from cultured lymphocytes with drug treatment. The Basic Protocol 2, high-resolution FISH mapping with free chromatin, is a modification of the method used for FISH mapping of interphase nuclei.en_HK
dc.languageeng-
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.does.org/masterli/cphg.html-
dc.relation.ispartofCurrent protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.]en_HK
dc.rightsCurrent protocols in human genetics. Copyright © John Wiley & Sons, Inc.-
dc.subject.meshCHO cells-
dc.subject.meshChromatin/genetics-
dc.subject.meshCricetulus-
dc.subject.meshDNA probe-
dc.subject.meshIn Situ Hybridization, Fluorescence/methods-
dc.titleHigh-resolution FISH analysis.en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1934-8258&volume=Chapter 4, Unit 4.5&spage=4.5.1&epage=4.5.23&date=2005&atitle=High-resolution+FISH+analysis-
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/0471142905.hg0405s44-
dc.identifier.pmid18428380-
dc.identifier.scopuseid_2-s2.0-48849089116en_HK
dc.identifier.volumeChapter 4en_HK
dc.identifier.spageUnit 4.5en_HK
dc.identifier.epageUnit 4.5en_HK
dc.identifier.scopusauthoridHeng, HH=7005338076en_HK
dc.identifier.scopusauthoridWindle, B=7003300791en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK

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