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Article: The cross-talk between osteoclasts and osteoblasts in response to strontium treatment: involvement of osteoprotegerin
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TitleThe cross-talk between osteoclasts and osteoblasts in response to strontium treatment: involvement of osteoprotegerin
 
AuthorsPeng, S1 3 2
Liu, XS2
Huang, S3
Li, Z3
Pan, H3
Zhen, W1
Luk, KDK3
Guo, XE2
Lu, WW3
 
Issue Date2011
 
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/bone
 
CitationBone, 2011, v. 49 n. 6, p. 1290-1298 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.bone.2011.08.031
 
AbstractBACKGROUND: The mechanism for the uncoupling effects of Sr on bone remains to be evaluated. Osteoblasts play important roles in osteoclastogenesis through regulating receptor activated nuclear factor kappa B (RANK) ligand (RANKL) and osteoprotegerin (OPG) expression. We hypothesize that OPG plays an important role in the cross-talk between osteoclasts and osteoblasts in response to Sr treatment. MATERIALS AND METHODS: MC3T3E1 cells were treated with Sr chloride (0-3 mM) and conditioned media were collected at 24h after the treatment. The effect of conditioned media on osteoclastogenesis was evaluated by tartrate-resistant acid phosphatase (TRAP) staining and bone resorption pits analysis. OPG and RANKL mRNA expressions in osteoblastic cells and protein secretion in the conditioned media were analyzed with real-time PCR and ELISA assay, respectively. The role of OPG in Sr-mediated inhibition of osteoclastogenesis was further evaluated with anti-OPG antibody in pre-osteoclastic cells. The role of OPG in Sr-mediated uncoupling effects on osteoporotic bone was evaluated by an animal study. Ovariectomized rats were oral administrated with vehicle or Sr chloride for two months supplemented with anti-IgG antibody (control) or anti-OPG antibody. The effects of OPG neutralization after Sr treatment on bone metabolism were analyzed by microCT, bone histomorphometry and biochemical analysis. RESULTS: The conditioned media derived from Sr-treated osteoblastic cells exerted a dose-dependent inhibitory effect on osteoclastic differentiation and resorptive activity in pre-osteoclastic cells. OPG mRNA expression and protein secretion in osteoblastic cells were significantly increased after Sr treatment. Neutralization with anti-OPG antibody abolished the inhibitory effect of conditioned media on RANKL-induced osteoclastogenesis. The uncoupling effects of Sr treatment on trabecular bone were evidenced by greater bone volume and trabecular number, greater osteoid surface and bone formation rate, while less osteoclast surface. These effects were attenuated by the OPG neutralization by anti-OPG antibody injection. CONCLUSION: The evidences from the in vitro and in vivo studies suggested that OPG played an important role in the uncoupling effect of Sr on bone metabolism, possibly by acting as a cross-talk molecule between osteoclasts and osteoblasts in response to Sr treatment.
 
ISSN8756-3282
2012 Impact Factor: 3.823
2012 SCImago Journal Rankings: 1.315
 
DOIhttp://dx.doi.org/10.1016/j.bone.2011.08.031
 
ISI Accession Number IDWOS:000297663500021
Funding AgencyGrant Number
Innovation and Technology Fund (ITF)GHP/009/06
Hong Kong Research Grants Council (RGC)714908
National Institutes of HealthR01 AR051376
Funding Information:

This study was supported by the Innovation and Technology Fund (ITF; Project ref. no. GHP/009/06), Hong Kong Research Grants Council (RGC) Grant 714908 and National Institutes of Health (R01 AR051376).

 
ReferencesReferences in Scopus
 
GrantsOptimization and commercialization of strontium containing bioactive bone cement for various orthopaedic applications
 
DC FieldValue
dc.contributor.authorPeng, S
 
dc.contributor.authorLiu, XS
 
dc.contributor.authorHuang, S
 
dc.contributor.authorLi, Z
 
dc.contributor.authorPan, H
 
dc.contributor.authorZhen, W
 
dc.contributor.authorLuk, KDK
 
dc.contributor.authorGuo, XE
 
dc.contributor.authorLu, WW
 
dc.date.accessioned2011-11-02T03:05:44Z
 
dc.date.available2011-11-02T03:05:44Z
 
dc.date.issued2011
 
dc.description.abstractBACKGROUND: The mechanism for the uncoupling effects of Sr on bone remains to be evaluated. Osteoblasts play important roles in osteoclastogenesis through regulating receptor activated nuclear factor kappa B (RANK) ligand (RANKL) and osteoprotegerin (OPG) expression. We hypothesize that OPG plays an important role in the cross-talk between osteoclasts and osteoblasts in response to Sr treatment. MATERIALS AND METHODS: MC3T3E1 cells were treated with Sr chloride (0-3 mM) and conditioned media were collected at 24h after the treatment. The effect of conditioned media on osteoclastogenesis was evaluated by tartrate-resistant acid phosphatase (TRAP) staining and bone resorption pits analysis. OPG and RANKL mRNA expressions in osteoblastic cells and protein secretion in the conditioned media were analyzed with real-time PCR and ELISA assay, respectively. The role of OPG in Sr-mediated inhibition of osteoclastogenesis was further evaluated with anti-OPG antibody in pre-osteoclastic cells. The role of OPG in Sr-mediated uncoupling effects on osteoporotic bone was evaluated by an animal study. Ovariectomized rats were oral administrated with vehicle or Sr chloride for two months supplemented with anti-IgG antibody (control) or anti-OPG antibody. The effects of OPG neutralization after Sr treatment on bone metabolism were analyzed by microCT, bone histomorphometry and biochemical analysis. RESULTS: The conditioned media derived from Sr-treated osteoblastic cells exerted a dose-dependent inhibitory effect on osteoclastic differentiation and resorptive activity in pre-osteoclastic cells. OPG mRNA expression and protein secretion in osteoblastic cells were significantly increased after Sr treatment. Neutralization with anti-OPG antibody abolished the inhibitory effect of conditioned media on RANKL-induced osteoclastogenesis. The uncoupling effects of Sr treatment on trabecular bone were evidenced by greater bone volume and trabecular number, greater osteoid surface and bone formation rate, while less osteoclast surface. These effects were attenuated by the OPG neutralization by anti-OPG antibody injection. CONCLUSION: The evidences from the in vitro and in vivo studies suggested that OPG played an important role in the uncoupling effect of Sr on bone metabolism, possibly by acting as a cross-talk molecule between osteoclasts and osteoblasts in response to Sr treatment.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationBone, 2011, v. 49 n. 6, p. 1290-1298 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.bone.2011.08.031
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.bone.2011.08.031
 
dc.identifier.epage1298
 
dc.identifier.hkuros207343
 
dc.identifier.isiWOS:000297663500021
Funding AgencyGrant Number
Innovation and Technology Fund (ITF)GHP/009/06
Hong Kong Research Grants Council (RGC)714908
National Institutes of HealthR01 AR051376
Funding Information:

This study was supported by the Innovation and Technology Fund (ITF; Project ref. no. GHP/009/06), Hong Kong Research Grants Council (RGC) Grant 714908 and National Institutes of Health (R01 AR051376).

 
dc.identifier.issn8756-3282
2012 Impact Factor: 3.823
2012 SCImago Journal Rankings: 1.315
 
dc.identifier.issue6
 
dc.identifier.pmid21925296
 
dc.identifier.scopuseid_2-s2.0-81355127486
 
dc.identifier.spage1290
 
dc.identifier.urihttp://hdl.handle.net/10722/143128
 
dc.identifier.volume49
 
dc.languageeng
 
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/bone
 
dc.publisher.placeUnited States
 
dc.relation.ispartofBone
 
dc.relation.projectOptimization and commercialization of strontium containing bioactive bone cement for various orthopaedic applications
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAnabolic Agents - pharmacology
 
dc.subject.meshOsteoblasts - drug effects - metabolism - pathology
 
dc.subject.meshOsteoclasts - drug effects - metabolism - pathology
 
dc.subject.meshOsteoprotegerin - genetics - metabolism
 
dc.subject.meshStrontium - pharmacology - therapeutic use
 
dc.titleThe cross-talk between osteoclasts and osteoblasts in response to strontium treatment: involvement of osteoprotegerin
 
dc.typeArticle
 
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<contributor.author>Huang, S</contributor.author>
<contributor.author>Li, Z</contributor.author>
<contributor.author>Pan, H</contributor.author>
<contributor.author>Zhen, W</contributor.author>
<contributor.author>Luk, KDK</contributor.author>
<contributor.author>Guo, XE</contributor.author>
<contributor.author>Lu, WW</contributor.author>
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<description.abstract>BACKGROUND: The mechanism for the uncoupling effects of Sr on bone remains to be evaluated. Osteoblasts play important roles in osteoclastogenesis through regulating receptor activated nuclear factor kappa B (RANK) ligand (RANKL) and osteoprotegerin (OPG) expression. We hypothesize that OPG plays an important role in the cross-talk between osteoclasts and osteoblasts in response to Sr treatment. MATERIALS AND METHODS: MC3T3E1 cells were treated with Sr chloride (0-3 mM) and conditioned media were collected at 24h after the treatment. The effect of conditioned media on osteoclastogenesis was evaluated by tartrate-resistant acid phosphatase (TRAP) staining and bone resorption pits analysis. OPG and RANKL mRNA expressions in osteoblastic cells and protein secretion in the conditioned media were analyzed with real-time PCR and ELISA assay, respectively. The role of OPG in Sr-mediated inhibition of osteoclastogenesis was further evaluated with anti-OPG antibody in pre-osteoclastic cells. The role of OPG in Sr-mediated uncoupling effects on osteoporotic bone was evaluated by an animal study. Ovariectomized rats were oral administrated with vehicle or Sr chloride for two months supplemented with anti-IgG antibody (control) or anti-OPG antibody. The effects of OPG neutralization after Sr treatment on bone metabolism were analyzed by microCT, bone histomorphometry and biochemical analysis. RESULTS: The conditioned media derived from Sr-treated osteoblastic cells exerted a dose-dependent inhibitory effect on osteoclastic differentiation and resorptive activity in pre-osteoclastic cells. OPG mRNA expression and protein secretion in osteoblastic cells were significantly increased after Sr treatment. Neutralization with anti-OPG antibody abolished the inhibitory effect of conditioned media on RANKL-induced osteoclastogenesis. The uncoupling effects of Sr treatment on trabecular bone were evidenced by greater bone volume and trabecular number, greater osteoid surface and bone formation rate, while less osteoclast surface. These effects were attenuated by the OPG neutralization by anti-OPG antibody injection. CONCLUSION: The evidences from the in vitro and in vivo studies suggested that OPG played an important role in the uncoupling effect of Sr on bone metabolism, possibly by acting as a cross-talk molecule between osteoclasts and osteoblasts in response to Sr treatment.</description.abstract>
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Author Affiliations
  1. Jinan University, School of Medicine
  2. Columbia University in the City of New York
  3. The University of Hong Kong