File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: The cross-talk between osteoclasts and osteoblasts in response to strontium treatment: involvement of osteoprotegerin

TitleThe cross-talk between osteoclasts and osteoblasts in response to strontium treatment: involvement of osteoprotegerin
Authors
Issue Date2011
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/bone
Citation
Bone, 2011, v. 49 n. 6, p. 1290-1298 How to Cite?
AbstractBACKGROUND: The mechanism for the uncoupling effects of Sr on bone remains to be evaluated. Osteoblasts play important roles in osteoclastogenesis through regulating receptor activated nuclear factor kappa B (RANK) ligand (RANKL) and osteoprotegerin (OPG) expression. We hypothesize that OPG plays an important role in the cross-talk between osteoclasts and osteoblasts in response to Sr treatment. MATERIALS AND METHODS: MC3T3E1 cells were treated with Sr chloride (0-3 mM) and conditioned media were collected at 24h after the treatment. The effect of conditioned media on osteoclastogenesis was evaluated by tartrate-resistant acid phosphatase (TRAP) staining and bone resorption pits analysis. OPG and RANKL mRNA expressions in osteoblastic cells and protein secretion in the conditioned media were analyzed with real-time PCR and ELISA assay, respectively. The role of OPG in Sr-mediated inhibition of osteoclastogenesis was further evaluated with anti-OPG antibody in pre-osteoclastic cells. The role of OPG in Sr-mediated uncoupling effects on osteoporotic bone was evaluated by an animal study. Ovariectomized rats were oral administrated with vehicle or Sr chloride for two months supplemented with anti-IgG antibody (control) or anti-OPG antibody. The effects of OPG neutralization after Sr treatment on bone metabolism were analyzed by microCT, bone histomorphometry and biochemical analysis. RESULTS: The conditioned media derived from Sr-treated osteoblastic cells exerted a dose-dependent inhibitory effect on osteoclastic differentiation and resorptive activity in pre-osteoclastic cells. OPG mRNA expression and protein secretion in osteoblastic cells were significantly increased after Sr treatment. Neutralization with anti-OPG antibody abolished the inhibitory effect of conditioned media on RANKL-induced osteoclastogenesis. The uncoupling effects of Sr treatment on trabecular bone were evidenced by greater bone volume and trabecular number, greater osteoid surface and bone formation rate, while less osteoclast surface. These effects were attenuated by the OPG neutralization by anti-OPG antibody injection. CONCLUSION: The evidences from the in vitro and in vivo studies suggested that OPG played an important role in the uncoupling effect of Sr on bone metabolism, possibly by acting as a cross-talk molecule between osteoclasts and osteoblasts in response to Sr treatment.
Persistent Identifierhttp://hdl.handle.net/10722/143128
ISSN
2014 Impact Factor: 3.973
2014 SCImago Journal Rankings: 1.469
ISI Accession Number ID
Funding AgencyGrant Number
Innovation and Technology Fund (ITF)GHP/009/06
Hong Kong Research Grants Council (RGC)714908
National Institutes of HealthR01 AR051376
Funding Information:

This study was supported by the Innovation and Technology Fund (ITF; Project ref. no. GHP/009/06), Hong Kong Research Grants Council (RGC) Grant 714908 and National Institutes of Health (R01 AR051376).

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorPeng, Sen_HK
dc.contributor.authorLiu, XSen_HK
dc.contributor.authorHuang, Sen_HK
dc.contributor.authorLi, Zen_HK
dc.contributor.authorPan, Hen_HK
dc.contributor.authorZhen, Wen_HK
dc.contributor.authorLuk, KDKen_HK
dc.contributor.authorGuo, XEen_HK
dc.contributor.authorLu, WWen_HK
dc.date.accessioned2011-11-02T03:05:44Z-
dc.date.available2011-11-02T03:05:44Z-
dc.date.issued2011en_HK
dc.identifier.citationBone, 2011, v. 49 n. 6, p. 1290-1298en_HK
dc.identifier.issn8756-3282en_HK
dc.identifier.urihttp://hdl.handle.net/10722/143128-
dc.description.abstractBACKGROUND: The mechanism for the uncoupling effects of Sr on bone remains to be evaluated. Osteoblasts play important roles in osteoclastogenesis through regulating receptor activated nuclear factor kappa B (RANK) ligand (RANKL) and osteoprotegerin (OPG) expression. We hypothesize that OPG plays an important role in the cross-talk between osteoclasts and osteoblasts in response to Sr treatment. MATERIALS AND METHODS: MC3T3E1 cells were treated with Sr chloride (0-3 mM) and conditioned media were collected at 24h after the treatment. The effect of conditioned media on osteoclastogenesis was evaluated by tartrate-resistant acid phosphatase (TRAP) staining and bone resorption pits analysis. OPG and RANKL mRNA expressions in osteoblastic cells and protein secretion in the conditioned media were analyzed with real-time PCR and ELISA assay, respectively. The role of OPG in Sr-mediated inhibition of osteoclastogenesis was further evaluated with anti-OPG antibody in pre-osteoclastic cells. The role of OPG in Sr-mediated uncoupling effects on osteoporotic bone was evaluated by an animal study. Ovariectomized rats were oral administrated with vehicle or Sr chloride for two months supplemented with anti-IgG antibody (control) or anti-OPG antibody. The effects of OPG neutralization after Sr treatment on bone metabolism were analyzed by microCT, bone histomorphometry and biochemical analysis. RESULTS: The conditioned media derived from Sr-treated osteoblastic cells exerted a dose-dependent inhibitory effect on osteoclastic differentiation and resorptive activity in pre-osteoclastic cells. OPG mRNA expression and protein secretion in osteoblastic cells were significantly increased after Sr treatment. Neutralization with anti-OPG antibody abolished the inhibitory effect of conditioned media on RANKL-induced osteoclastogenesis. The uncoupling effects of Sr treatment on trabecular bone were evidenced by greater bone volume and trabecular number, greater osteoid surface and bone formation rate, while less osteoclast surface. These effects were attenuated by the OPG neutralization by anti-OPG antibody injection. CONCLUSION: The evidences from the in vitro and in vivo studies suggested that OPG played an important role in the uncoupling effect of Sr on bone metabolism, possibly by acting as a cross-talk molecule between osteoclasts and osteoblasts in response to Sr treatment.en_HK
dc.languageengen_US
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/boneen_HK
dc.relation.ispartofBoneen_HK
dc.subject.meshAnabolic Agents - pharmacology-
dc.subject.meshOsteoblasts - drug effects - metabolism - pathology-
dc.subject.meshOsteoclasts - drug effects - metabolism - pathology-
dc.subject.meshOsteoprotegerin - genetics - metabolism-
dc.subject.meshStrontium - pharmacology - therapeutic use-
dc.titleThe cross-talk between osteoclasts and osteoblasts in response to strontium treatment: involvement of osteoprotegerinen_HK
dc.typeArticleen_HK
dc.identifier.emailPeng, S: songlin@hku.hken_HK
dc.identifier.emailLi, Z: lizy@hku.hken_HK
dc.identifier.emailPan, H: haobo@hku.hken_HK
dc.identifier.emailZhen, W: spine.zhen@gmail.com-
dc.identifier.emailLuk, KDK: hcm21000@hku.hk-
dc.identifier.emailLu, WW: wwlu@hku.hk-
dc.identifier.authorityPan, H=rp01564en_HK
dc.identifier.authorityLuk, KDK=rp00333en_HK
dc.identifier.authorityLu, WW=rp00411en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.bone.2011.08.031en_HK
dc.identifier.pmid21925296en_HK
dc.identifier.scopuseid_2-s2.0-81355127486en_HK
dc.identifier.hkuros207343-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-81355127486&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume49en_HK
dc.identifier.issue6en_HK
dc.identifier.spage1290en_HK
dc.identifier.epage1298en_HK
dc.identifier.isiWOS:000297663500021-
dc.publisher.placeUnited Statesen_HK
dc.relation.projectOptimization and commercialization of strontium containing bioactive bone cement for various orthopaedic applications-
dc.identifier.scopusauthoridLu, WW=7404215221en_HK
dc.identifier.scopusauthoridGuo, XE=35237105200en_HK
dc.identifier.scopusauthoridLuk, KDK=7201921573en_HK
dc.identifier.scopusauthoridZhen, W=9044966900en_HK
dc.identifier.scopusauthoridPan, H=7403295092en_HK
dc.identifier.scopusauthoridLi, Z=35784563200en_HK
dc.identifier.scopusauthoridHuang, S=54581004600en_HK
dc.identifier.scopusauthoridLiu, XS=50061438600en_HK
dc.identifier.scopusauthoridPeng, S=13402746900en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats