File Download
  Links for fulltext
     (May Require Subscription)
  • Find via Find It@HKUL

Conference Paper: Zygotic increase in microRNA-34c is required for the first cleavage division in mouse

TitleZygotic increase in microRNA-34c is required for the first cleavage division in mouse
Authors
KeywordsBiology
Issue Date2010
PublisherSociety for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/
Citation
The 43rd Annual Meeting of the Society for the Study of Reproduction, Milwaukee, WI, 30 July-3 August 2010. In Biology of Reproduction, v. 83 Meeting Abstracts, p. 104, abstract no. 239 How to Cite?
AbstractMicroRNAs (miRNAs) are small noncoding RNAs that regulate manybiological processes via gene silencing and/or transcript degradation.Their precise role(s) in preimplantation embryo developmentremains largely unclear. Here, we describe the miRNA expressionprofile of mouse preimplantation embryos by multiplex real-timepolymerase chain reaction and identify 16 miRNAs that are specificallyup-regulated in the zygotes. One of these miRNAs is miR-34c,which is weakly expressed in the oocytes but is increased by4-fold in the zygotes. Microinjection of miR-34c inhibitor,but not non-specific miRNA inhibitor in zygotes leads to aninhibition in DNA synthesis and significant suppression of thefirst cleavage division. 3'UTR luciferase assay and Westernblotting with or without inhibition of miR-34c showed that miR-34cregulates the expression of Bcl-2 in the zygotes and the trophoblastcell line JAR. Microinjection of anti-Bcl-2 antibody to zygotespartially reverses, while microinjection of Bcl-2 protein mimicsthe effect of miR-34c inhibition. In addition, microinjectionof either miR-34c inhibitor or Bcl-2 reduced c-myc expression,which is known to be important for early embryonic development.Our findings provides direct evidence that miR-34c is importantfor zygotic development and that Bcl-2 and c-myc are downstreammolecules of miR-34c modulating the first cleavage division.
DescriptionConference Theme: The Intersection Between Genetics, Genomics, and Reproductive Biology
Poster
Open Access Journal
Persistent Identifierhttp://hdl.handle.net/10722/142979
ISSN
2015 Impact Factor: 3.471
2015 SCImago Journal Rankings: 1.646

 

DC FieldValueLanguage
dc.contributor.authorLiu, Wen_US
dc.contributor.authorPang, RTKen_US
dc.contributor.authorChiu, PCNen_US
dc.contributor.authorWong, BPCen_US
dc.contributor.authorLao, KQen_US
dc.contributor.authorLee, KFen_US
dc.contributor.authorYeung, WSBen_US
dc.date.accessioned2011-10-28T03:00:46Z-
dc.date.available2011-10-28T03:00:46Z-
dc.date.issued2010en_US
dc.identifier.citationThe 43rd Annual Meeting of the Society for the Study of Reproduction, Milwaukee, WI, 30 July-3 August 2010. In Biology of Reproduction, v. 83 Meeting Abstracts, p. 104, abstract no. 239en_US
dc.identifier.issn0006-3363-
dc.identifier.urihttp://hdl.handle.net/10722/142979-
dc.descriptionConference Theme: The Intersection Between Genetics, Genomics, and Reproductive Biology-
dc.descriptionPoster-
dc.descriptionOpen Access Journal-
dc.description.abstractMicroRNAs (miRNAs) are small noncoding RNAs that regulate manybiological processes via gene silencing and/or transcript degradation.Their precise role(s) in preimplantation embryo developmentremains largely unclear. Here, we describe the miRNA expressionprofile of mouse preimplantation embryos by multiplex real-timepolymerase chain reaction and identify 16 miRNAs that are specificallyup-regulated in the zygotes. One of these miRNAs is miR-34c,which is weakly expressed in the oocytes but is increased by4-fold in the zygotes. Microinjection of miR-34c inhibitor,but not non-specific miRNA inhibitor in zygotes leads to aninhibition in DNA synthesis and significant suppression of thefirst cleavage division. 3'UTR luciferase assay and Westernblotting with or without inhibition of miR-34c showed that miR-34cregulates the expression of Bcl-2 in the zygotes and the trophoblastcell line JAR. Microinjection of anti-Bcl-2 antibody to zygotespartially reverses, while microinjection of Bcl-2 protein mimicsthe effect of miR-34c inhibition. In addition, microinjectionof either miR-34c inhibitor or Bcl-2 reduced c-myc expression,which is known to be important for early embryonic development.Our findings provides direct evidence that miR-34c is importantfor zygotic development and that Bcl-2 and c-myc are downstreammolecules of miR-34c modulating the first cleavage division.-
dc.languageengen_US
dc.publisherSociety for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/en_US
dc.relation.ispartofBiology of Reproductionen_US
dc.subjectBiology-
dc.titleZygotic increase in microRNA-34c is required for the first cleavage division in mouseen_US
dc.typeConference_Paperen_US
dc.identifier.emailLiu, W: liuwm@hkucc.hku.hken_US
dc.identifier.emailPang, RTK: rtkpang@hku.hken_US
dc.identifier.emailChiu, PCN: pchiucn@hku.hken_US
dc.identifier.emailWong, BPC: bpcwong@hkucc.hku.hken_US
dc.identifier.emailLee, KF: ckflee@hku.hken_US
dc.identifier.emailYeung, WSB: wsbyeung@hkucc.hku.hk-
dc.identifier.authorityPang, RTK=rp01761en_US
dc.identifier.authorityChiu, PCN=rp00424en_US
dc.identifier.authorityLee, KF=rp00458en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.hkuros184337en_US
dc.identifier.volume83en_US
dc.identifier.issueMeeting Abstracts-
dc.identifier.spage104en_US
dc.identifier.epage104en_US
dc.publisher.placeUnited States-
dc.customcontrol.immutablesml 130715-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats