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Article: Hsa-let-7g inhibits proliferation of hepatocellular carcinoma cells by downregulation of c-Myc and upregulation of p16 INK4A
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TitleHsa-let-7g inhibits proliferation of hepatocellular carcinoma cells by downregulation of c-Myc and upregulation of p16 INK4A
 
AuthorsLan, FF2
Wang, H3
Chen, YC3
Chan, CY3
Ng, SS1
Li, K2
Xie, D2
He, ML3
Lin, MC3
Kung, HF3 2
 
Keywordsc-Myc
hepatocellular carcinoma
hsa-let-7g
p16 INK4A
 
Issue Date2011
 
PublisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/home
 
CitationInternational Journal Of Cancer, 2011, v. 128 n. 2, p. 319-331 [How to Cite?]
DOI: http://dx.doi.org/10.1002/ijc.25336
 
AbstractzMicroRNAs (miRNAs) are endogenously expressed small noncoding RNAs that regulate approximately one-third of human genes at post-transcription level. Previous studies have shown that miRNAs were implicated in many cellular processes and participated in the progress of various tumors including hepatocellular carcinoma (HCC). Among all miRNAs, the let-7 family is well recognized to play pivotal roles in tumorigenesis by functioning as potential growth suppressor. In the present study, we aimed to investigate the role of let-7 family, particularly the hsa-let-7g, in the molecular pathogenesis of HCC. By use of MTT, qPCR, Western blotting and 2-dimensional electrophoresis (2-DE), over-expression of hsa-let-7g was found to inhibit the proliferation of HCC cell line via negative and positive regulations of c-Myc and p16 INK4A, respectively. The expression of hsa-let-7g was noted to be markedly lowered in the HepG2, Hep3B and Huh7 cells, yet higher in the Bel-7404 HCC cell line. Proliferation of HCC cell line was significantly inhibited after the transfection of hsa-let-7g mimics, while hsa-let-7g inhibitor transfection exerted an opposite effect. Concurrently, the mRNA and protein levels of c-Myc were found significantly decreased in HepG2 cells after transfection of hsa-let-7g mimics, but obviously increased in Bel-7404 cells after transfection of hsa-let-7g inhibitor. As revealed by 2-DE, a significant upregulation of p16 INK4A was revealed after the gain-of-function study using hsa-let-7g. Therefore, we suggest that hsa-let-7g may act as a tumor suppressor gene that inhibits HCC cell proliferation by downregulating the oncogene, c-Myc, and upregulating the tumor suppressor gene, p16 INK4A. Copyright © 2010 UICC.
 
ISSN0020-7136
2012 Impact Factor: 6.198
2012 SCImago Journal Rankings: 2.309
 
DOIhttp://dx.doi.org/10.1002/ijc.25336
 
ISI Accession Number IDWOS:000285263100008
Funding AgencyGrant Number
National Basic Research Program of China2010CB912800
Funding Information:

National Basic Research Program of China; Grant number: 2010CB912800

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorLan, FF
 
dc.contributor.authorWang, H
 
dc.contributor.authorChen, YC
 
dc.contributor.authorChan, CY
 
dc.contributor.authorNg, SS
 
dc.contributor.authorLi, K
 
dc.contributor.authorXie, D
 
dc.contributor.authorHe, ML
 
dc.contributor.authorLin, MC
 
dc.contributor.authorKung, HF
 
dc.date.accessioned2011-10-28T03:00:29Z
 
dc.date.available2011-10-28T03:00:29Z
 
dc.date.issued2011
 
dc.description.abstractzMicroRNAs (miRNAs) are endogenously expressed small noncoding RNAs that regulate approximately one-third of human genes at post-transcription level. Previous studies have shown that miRNAs were implicated in many cellular processes and participated in the progress of various tumors including hepatocellular carcinoma (HCC). Among all miRNAs, the let-7 family is well recognized to play pivotal roles in tumorigenesis by functioning as potential growth suppressor. In the present study, we aimed to investigate the role of let-7 family, particularly the hsa-let-7g, in the molecular pathogenesis of HCC. By use of MTT, qPCR, Western blotting and 2-dimensional electrophoresis (2-DE), over-expression of hsa-let-7g was found to inhibit the proliferation of HCC cell line via negative and positive regulations of c-Myc and p16 INK4A, respectively. The expression of hsa-let-7g was noted to be markedly lowered in the HepG2, Hep3B and Huh7 cells, yet higher in the Bel-7404 HCC cell line. Proliferation of HCC cell line was significantly inhibited after the transfection of hsa-let-7g mimics, while hsa-let-7g inhibitor transfection exerted an opposite effect. Concurrently, the mRNA and protein levels of c-Myc were found significantly decreased in HepG2 cells after transfection of hsa-let-7g mimics, but obviously increased in Bel-7404 cells after transfection of hsa-let-7g inhibitor. As revealed by 2-DE, a significant upregulation of p16 INK4A was revealed after the gain-of-function study using hsa-let-7g. Therefore, we suggest that hsa-let-7g may act as a tumor suppressor gene that inhibits HCC cell proliferation by downregulating the oncogene, c-Myc, and upregulating the tumor suppressor gene, p16 INK4A. Copyright © 2010 UICC.
 
dc.description.naturelink_to_OA_fulltext
 
dc.identifier.citationInternational Journal Of Cancer, 2011, v. 128 n. 2, p. 319-331 [How to Cite?]
DOI: http://dx.doi.org/10.1002/ijc.25336
 
dc.identifier.doihttp://dx.doi.org/10.1002/ijc.25336
 
dc.identifier.epage331
 
dc.identifier.hkuros196607
 
dc.identifier.isiWOS:000285263100008
Funding AgencyGrant Number
National Basic Research Program of China2010CB912800
Funding Information:

National Basic Research Program of China; Grant number: 2010CB912800

 
dc.identifier.issn0020-7136
2012 Impact Factor: 6.198
2012 SCImago Journal Rankings: 2.309
 
dc.identifier.issue2
 
dc.identifier.pmid20309945
 
dc.identifier.scopuseid_2-s2.0-78649561463
 
dc.identifier.spage319
 
dc.identifier.urihttp://hdl.handle.net/10722/142972
 
dc.identifier.volume128
 
dc.languageeng
 
dc.publisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/home
 
dc.publisher.placeUnited States
 
dc.relation.ispartofInternational Journal of Cancer
 
dc.relation.referencesReferences in Scopus
 
dc.rightsInternational Journal of Cancer. Copyright © John Wiley & Sons, Inc..
 
dc.subject.meshCarcinoma, Hepatocellular - pathology - prevention and control
 
dc.subject.meshGene Expression Regulation, Neoplastic
 
dc.subject.meshLiver Neoplasms - pathology - prevention and control
 
dc.subject.meshMicroRNAs - genetics - physiology
 
dc.subject.meshProto-Oncogene Proteins c-myc - genetics
 
dc.subjectc-Myc
 
dc.subjecthepatocellular carcinoma
 
dc.subjecthsa-let-7g
 
dc.subjectp16 INK4A
 
dc.titleHsa-let-7g inhibits proliferation of hepatocellular carcinoma cells by downregulation of c-Myc and upregulation of p16 INK4A
 
dc.typeArticle
 
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<contributor.author>Ng, SS</contributor.author>
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Author Affiliations
  1. The University of Hong Kong
  2. Sun Yat-Sen University
  3. Chinese University of Hong Kong