Article: Characterization of an alkane-degrading methanogenic enrichment culture from production water of an oil reservoir after 274 days of incubation
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- Publisher Website: 10.1016/j.ibiod.2010.12.010
- Scopus: eid_2-s2.0-79953105507
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| Title | Characterization of an alkane-degrading methanogenic enrichment culture from production water of an oil reservoir after 274 days of incubation | ||||||
|---|---|---|---|---|---|---|---|
| Authors | Wang, LY2 Gao, CX2 Mbadinga, SM2 Zhou, L2 Liu, JF2 Gu, JD1 Mu, BZ2 | ||||||
| Keywords | Methanogenesis Microbial community N-alkanes Oil reservoir Production water | ||||||
| Issue Date | 2011 | ||||||
| Publisher | Elsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/ibiod | ||||||
| Citation | International Biodeterioration And Biodegradation, 2011, v. 65 n. 3, p. 444-450 [How to Cite?] DOI: http://dx.doi.org/10.1016/j.ibiod.2010.12.010 | ||||||
| Abstract | Oil reservoirs represent special habitats for the activity of anaerobic microbial communities in the transformation of organic compounds. To understand the function of microbial communities in oil reservoirs under anaerobic conditions, an alkane-degrading methanogenic enrichment culture was established and analyzed. Results showed that a net 538 μmol of methane higher than the controls were produced over 274 days of incubation in microcosms amended with alkanes and a decrease in the alkanes profile was also observed. Phylogenetic analysis of 16S rRNA gene sequences retrieved from the enrichment microcosms indicated that the archaeal phylotypes were mostly related to members of the orders Methanobacteriales and Methanosarcinales. The bacterial clone library was composed of sequences affiliated with the Firmicutes, Proteobacteria, Deferribacteres, and Bacteroidetes. However, most of the bacterial clones retrieved from the enrichment cultures showed low similarity to 16S rRNA gene sequences of the cultured members, indicating that the enrichment cultures contained novel bacterial species. Though alkane-degrading methanogenic enrichment consortium has rarely been reported from petroleum reservoirs, our results indicated that oilfield production water harbors a microbial community capable of syntrophic conversion of n-alkanes to methane, which sheds light on the bio-utilization of marginal oil reservoirs for enhanced energy recovery. © 2011 Elsevier Ltd. | ||||||
| ISSN | 0964-8305 2011 Impact Factor: 2.074 2011 SCImago Journal Rankings: 0.085 | ||||||
| DOI | http://dx.doi.org/10.1016/j.ibiod.2010.12.010 | ||||||
| ISI Accession Number ID | WOS:000290693900010
Funding Information: This work was supported by the National Natural Science Foundation of China (Grant No. 41073055) and the 863 Program (Grant No. 2009AA063503). | ||||||
| References | References in Scopus |
| dc.contributor.author | Wang, LY | ||||||
|---|---|---|---|---|---|---|---|
| dc.contributor.author | Gao, CX | ||||||
| dc.contributor.author | Mbadinga, SM | ||||||
| dc.contributor.author | Zhou, L | ||||||
| dc.contributor.author | Liu, JF | ||||||
| dc.contributor.author | Gu, JD | ||||||
| dc.contributor.author | Mu, BZ | ||||||
| dc.date.accessioned | 2011-10-28T03:00:22Z | ||||||
| dc.date.available | 2011-10-28T03:00:22Z | ||||||
| dc.date.issued | 2011 | ||||||
| dc.description.abstract | Oil reservoirs represent special habitats for the activity of anaerobic microbial communities in the transformation of organic compounds. To understand the function of microbial communities in oil reservoirs under anaerobic conditions, an alkane-degrading methanogenic enrichment culture was established and analyzed. Results showed that a net 538 μmol of methane higher than the controls were produced over 274 days of incubation in microcosms amended with alkanes and a decrease in the alkanes profile was also observed. Phylogenetic analysis of 16S rRNA gene sequences retrieved from the enrichment microcosms indicated that the archaeal phylotypes were mostly related to members of the orders Methanobacteriales and Methanosarcinales. The bacterial clone library was composed of sequences affiliated with the Firmicutes, Proteobacteria, Deferribacteres, and Bacteroidetes. However, most of the bacterial clones retrieved from the enrichment cultures showed low similarity to 16S rRNA gene sequences of the cultured members, indicating that the enrichment cultures contained novel bacterial species. Though alkane-degrading methanogenic enrichment consortium has rarely been reported from petroleum reservoirs, our results indicated that oilfield production water harbors a microbial community capable of syntrophic conversion of n-alkanes to methane, which sheds light on the bio-utilization of marginal oil reservoirs for enhanced energy recovery. © 2011 Elsevier Ltd. | ||||||
| dc.description.nature | Link_to_subscribed_fulltext | ||||||
| dc.identifier.citation | International Biodeterioration And Biodegradation, 2011, v. 65 n. 3, p. 444-450 [How to Cite?] DOI: http://dx.doi.org/10.1016/j.ibiod.2010.12.010 | ||||||
| dc.identifier.citeulike | 8885860 | ||||||
| dc.identifier.doi | http://dx.doi.org/10.1016/j.ibiod.2010.12.010 | ||||||
| dc.identifier.epage | 450 | ||||||
| dc.identifier.hkuros | 194846 | ||||||
| dc.identifier.isi | WOS:000290693900010
Funding Information: This work was supported by the National Natural Science Foundation of China (Grant No. 41073055) and the 863 Program (Grant No. 2009AA063503). | ||||||
| dc.identifier.issn | 0964-8305 2011 Impact Factor: 2.074 2011 SCImago Journal Rankings: 0.085 | ||||||
| dc.identifier.issue | 3 | ||||||
| dc.identifier.openurl | | ||||||
| dc.identifier.scopus | eid_2-s2.0-79953105507 | ||||||
| dc.identifier.spage | 444 | ||||||
| dc.identifier.uri | http://hdl.handle.net/10722/142970 | ||||||
| dc.identifier.volume | 65 | ||||||
| dc.language | eng | ||||||
| dc.publisher | Elsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/ibiod | ||||||
| dc.publisher.place | United Kingdom | ||||||
| dc.relation.ispartof | International Biodeterioration and Biodegradation | ||||||
| dc.relation.references | References in Scopus | ||||||
| dc.subject | Methanogenesis | ||||||
| dc.subject | Microbial community | ||||||
| dc.subject | N-alkanes | ||||||
| dc.subject | Oil reservoir | ||||||
| dc.subject | Production water | ||||||
| dc.title | Characterization of an alkane-degrading methanogenic enrichment culture from production water of an oil reservoir after 274 days of incubation | ||||||
| dc.type | Article |
Author Affiliations
- The University of Hong Kong
- East China University of Science and Technology