File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Characterization of an alkane-degrading methanogenic enrichment culture from production water of an oil reservoir after 274 days of incubation

TitleCharacterization of an alkane-degrading methanogenic enrichment culture from production water of an oil reservoir after 274 days of incubation
Authors
KeywordsMethanogenesis
Microbial community
N-alkanes
Oil reservoir
Production water
Issue Date2011
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/ibiod
Citation
International Biodeterioration And Biodegradation, 2011, v. 65 n. 3, p. 444-450 How to Cite?
AbstractOil reservoirs represent special habitats for the activity of anaerobic microbial communities in the transformation of organic compounds. To understand the function of microbial communities in oil reservoirs under anaerobic conditions, an alkane-degrading methanogenic enrichment culture was established and analyzed. Results showed that a net 538 μmol of methane higher than the controls were produced over 274 days of incubation in microcosms amended with alkanes and a decrease in the alkanes profile was also observed. Phylogenetic analysis of 16S rRNA gene sequences retrieved from the enrichment microcosms indicated that the archaeal phylotypes were mostly related to members of the orders Methanobacteriales and Methanosarcinales. The bacterial clone library was composed of sequences affiliated with the Firmicutes, Proteobacteria, Deferribacteres, and Bacteroidetes. However, most of the bacterial clones retrieved from the enrichment cultures showed low similarity to 16S rRNA gene sequences of the cultured members, indicating that the enrichment cultures contained novel bacterial species. Though alkane-degrading methanogenic enrichment consortium has rarely been reported from petroleum reservoirs, our results indicated that oilfield production water harbors a microbial community capable of syntrophic conversion of n-alkanes to methane, which sheds light on the bio-utilization of marginal oil reservoirs for enhanced energy recovery. © 2011 Elsevier Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/142970
ISSN
2014 Impact Factor: 2.131
2014 SCImago Journal Rankings: 0.788
ISI Accession Number ID
Funding AgencyGrant Number
National Natural Science Foundation of China41073055
863 Program2009AA063503
Funding Information:

This work was supported by the National Natural Science Foundation of China (Grant No. 41073055) and the 863 Program (Grant No. 2009AA063503).

References

 

DC FieldValueLanguage
dc.contributor.authorWang, LYen_HK
dc.contributor.authorGao, CXen_HK
dc.contributor.authorMbadinga, SMen_HK
dc.contributor.authorZhou, Len_HK
dc.contributor.authorLiu, JFen_HK
dc.contributor.authorGu, JDen_HK
dc.contributor.authorMu, BZen_HK
dc.date.accessioned2011-10-28T03:00:22Z-
dc.date.available2011-10-28T03:00:22Z-
dc.date.issued2011en_HK
dc.identifier.citationInternational Biodeterioration And Biodegradation, 2011, v. 65 n. 3, p. 444-450en_HK
dc.identifier.issn0964-8305en_HK
dc.identifier.urihttp://hdl.handle.net/10722/142970-
dc.description.abstractOil reservoirs represent special habitats for the activity of anaerobic microbial communities in the transformation of organic compounds. To understand the function of microbial communities in oil reservoirs under anaerobic conditions, an alkane-degrading methanogenic enrichment culture was established and analyzed. Results showed that a net 538 μmol of methane higher than the controls were produced over 274 days of incubation in microcosms amended with alkanes and a decrease in the alkanes profile was also observed. Phylogenetic analysis of 16S rRNA gene sequences retrieved from the enrichment microcosms indicated that the archaeal phylotypes were mostly related to members of the orders Methanobacteriales and Methanosarcinales. The bacterial clone library was composed of sequences affiliated with the Firmicutes, Proteobacteria, Deferribacteres, and Bacteroidetes. However, most of the bacterial clones retrieved from the enrichment cultures showed low similarity to 16S rRNA gene sequences of the cultured members, indicating that the enrichment cultures contained novel bacterial species. Though alkane-degrading methanogenic enrichment consortium has rarely been reported from petroleum reservoirs, our results indicated that oilfield production water harbors a microbial community capable of syntrophic conversion of n-alkanes to methane, which sheds light on the bio-utilization of marginal oil reservoirs for enhanced energy recovery. © 2011 Elsevier Ltd.en_HK
dc.languageengen_US
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/ibioden_HK
dc.relation.ispartofInternational Biodeterioration and Biodegradationen_HK
dc.subjectMethanogenesisen_HK
dc.subjectMicrobial communityen_HK
dc.subjectN-alkanesen_HK
dc.subjectOil reservoiren_HK
dc.subjectProduction wateren_HK
dc.titleCharacterization of an alkane-degrading methanogenic enrichment culture from production water of an oil reservoir after 274 days of incubationen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0964-8305&volume=65&spage=444&epage=450&date=2011&atitle=Characterization+of+an+alkane-degrading+methanogenic+enrichment+culture+from+production+water+of+oil+reservoir+after+274+days+of+incubationen_US
dc.identifier.emailGu, JD: jdgu@hkucc.hku.hken_HK
dc.identifier.authorityGu, JD=rp00701en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.ibiod.2010.12.010en_HK
dc.identifier.scopuseid_2-s2.0-79953105507en_HK
dc.identifier.hkuros194846en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79953105507&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume65en_HK
dc.identifier.issue3en_HK
dc.identifier.spage444en_HK
dc.identifier.epage450en_HK
dc.identifier.isiWOS:000290693900010-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridWang, LY=36803082300en_HK
dc.identifier.scopusauthoridGao, CX=37090498100en_HK
dc.identifier.scopusauthoridMbadinga, SM=36802004800en_HK
dc.identifier.scopusauthoridZhou, L=7404126715en_HK
dc.identifier.scopusauthoridLiu, JF=36096880500en_HK
dc.identifier.scopusauthoridGu, JD=7403129601en_HK
dc.identifier.scopusauthoridMu, BZ=7004585949en_HK
dc.identifier.citeulike8885860-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats