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Article: Characterization of an alkane-degrading methanogenic enrichment culture from production water of an oil reservoir after 274 days of incubation
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TitleCharacterization of an alkane-degrading methanogenic enrichment culture from production water of an oil reservoir after 274 days of incubation
 
AuthorsWang, LY2
Gao, CX2
Mbadinga, SM2
Zhou, L2
Liu, JF2
Gu, JD1
Mu, BZ2
 
KeywordsMethanogenesis
Microbial community
N-alkanes
Oil reservoir
Production water
 
Issue Date2011
 
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/ibiod
 
CitationInternational Biodeterioration And Biodegradation, 2011, v. 65 n. 3, p. 444-450 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.ibiod.2010.12.010
 
AbstractOil reservoirs represent special habitats for the activity of anaerobic microbial communities in the transformation of organic compounds. To understand the function of microbial communities in oil reservoirs under anaerobic conditions, an alkane-degrading methanogenic enrichment culture was established and analyzed. Results showed that a net 538 μmol of methane higher than the controls were produced over 274 days of incubation in microcosms amended with alkanes and a decrease in the alkanes profile was also observed. Phylogenetic analysis of 16S rRNA gene sequences retrieved from the enrichment microcosms indicated that the archaeal phylotypes were mostly related to members of the orders Methanobacteriales and Methanosarcinales. The bacterial clone library was composed of sequences affiliated with the Firmicutes, Proteobacteria, Deferribacteres, and Bacteroidetes. However, most of the bacterial clones retrieved from the enrichment cultures showed low similarity to 16S rRNA gene sequences of the cultured members, indicating that the enrichment cultures contained novel bacterial species. Though alkane-degrading methanogenic enrichment consortium has rarely been reported from petroleum reservoirs, our results indicated that oilfield production water harbors a microbial community capable of syntrophic conversion of n-alkanes to methane, which sheds light on the bio-utilization of marginal oil reservoirs for enhanced energy recovery. © 2011 Elsevier Ltd.
 
ISSN0964-8305
2013 Impact Factor: 2.235
2013 SCImago Journal Rankings: 0.884
 
DOIhttp://dx.doi.org/10.1016/j.ibiod.2010.12.010
 
ISI Accession Number IDWOS:000290693900010
Funding AgencyGrant Number
National Natural Science Foundation of China41073055
863 Program2009AA063503
Funding Information:

This work was supported by the National Natural Science Foundation of China (Grant No. 41073055) and the 863 Program (Grant No. 2009AA063503).

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorWang, LY
 
dc.contributor.authorGao, CX
 
dc.contributor.authorMbadinga, SM
 
dc.contributor.authorZhou, L
 
dc.contributor.authorLiu, JF
 
dc.contributor.authorGu, JD
 
dc.contributor.authorMu, BZ
 
dc.date.accessioned2011-10-28T03:00:22Z
 
dc.date.available2011-10-28T03:00:22Z
 
dc.date.issued2011
 
dc.description.abstractOil reservoirs represent special habitats for the activity of anaerobic microbial communities in the transformation of organic compounds. To understand the function of microbial communities in oil reservoirs under anaerobic conditions, an alkane-degrading methanogenic enrichment culture was established and analyzed. Results showed that a net 538 μmol of methane higher than the controls were produced over 274 days of incubation in microcosms amended with alkanes and a decrease in the alkanes profile was also observed. Phylogenetic analysis of 16S rRNA gene sequences retrieved from the enrichment microcosms indicated that the archaeal phylotypes were mostly related to members of the orders Methanobacteriales and Methanosarcinales. The bacterial clone library was composed of sequences affiliated with the Firmicutes, Proteobacteria, Deferribacteres, and Bacteroidetes. However, most of the bacterial clones retrieved from the enrichment cultures showed low similarity to 16S rRNA gene sequences of the cultured members, indicating that the enrichment cultures contained novel bacterial species. Though alkane-degrading methanogenic enrichment consortium has rarely been reported from petroleum reservoirs, our results indicated that oilfield production water harbors a microbial community capable of syntrophic conversion of n-alkanes to methane, which sheds light on the bio-utilization of marginal oil reservoirs for enhanced energy recovery. © 2011 Elsevier Ltd.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationInternational Biodeterioration And Biodegradation, 2011, v. 65 n. 3, p. 444-450 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.ibiod.2010.12.010
 
dc.identifier.citeulike8885860
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.ibiod.2010.12.010
 
dc.identifier.epage450
 
dc.identifier.hkuros194846
 
dc.identifier.isiWOS:000290693900010
Funding AgencyGrant Number
National Natural Science Foundation of China41073055
863 Program2009AA063503
Funding Information:

This work was supported by the National Natural Science Foundation of China (Grant No. 41073055) and the 863 Program (Grant No. 2009AA063503).

 
dc.identifier.issn0964-8305
2013 Impact Factor: 2.235
2013 SCImago Journal Rankings: 0.884
 
dc.identifier.issue3
 
dc.identifier.openurl
 
dc.identifier.scopuseid_2-s2.0-79953105507
 
dc.identifier.spage444
 
dc.identifier.urihttp://hdl.handle.net/10722/142970
 
dc.identifier.volume65
 
dc.languageeng
 
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/ibiod
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofInternational Biodeterioration and Biodegradation
 
dc.relation.referencesReferences in Scopus
 
dc.subjectMethanogenesis
 
dc.subjectMicrobial community
 
dc.subjectN-alkanes
 
dc.subjectOil reservoir
 
dc.subjectProduction water
 
dc.titleCharacterization of an alkane-degrading methanogenic enrichment culture from production water of an oil reservoir after 274 days of incubation
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong
  2. East China University of Science and Technology