File Download

There are no files associated with this item.

Supplementary

Conference Paper: Effects of detergents on bovine disc decullularization for the generation of natural disc scaffolds for intervertebral disc tissue engineering

TitleEffects of detergents on bovine disc decullularization for the generation of natural disc scaffolds for intervertebral disc tissue engineering
Authors
Issue Date2010
Citation
The 7th SICOT/SIROT Annual International Conference combined meeting with the Swedish Orthopaedic Association (SOF), Gothenburg, Sweden, 31 August-3 September 2010. How to Cite?
AbstractStudy of intervertebral disc (IVD) cells in a 3D formation within a niche resembling native extracellular matrix (ECM) is important for researchers to understand the actual cell behaviour and etiology of disc degeneration/regeneration. Removal of resident cells and cellular contents whilst minimizing adverse effects on ECM from IVD is necessary in making a natural scaffold. We hypothesize that bovine discs may be effectively decellularised using a detergent to produce a scaffold resembling native IVD for disc cell investigation. Bovine caudal discs (18-20mm diameter) were harvested and incubated with either 25 mL of PBS containing 0.1% SDS or 1% Triton X-100 (TX-100) in Tris-HCL solution with protease inhibitor. Live/Dead staining and Alamar Blue assay were used to determine numbers of resident cells and their metabolic activity after treatment, respectively.0.1% SDS solution enabled removal of 69% and 73% cell contents from annulus fibrosus and nucleus pulposus respectively, whereas 1% TX-100 removed 54% from both tissues. SDS was the most efficient in removing resident cells from IVD. Being an ionic reagent, SDS has a role in solubilising cytoplasmic and nuclear membranes, which caused the disruption of protein-protein interactions and aided in the clearance of cells. The successful development of a natural scaffold will allow IVD cells to be studied in a physiological 3D microenvironment without the use of in vivo animal models. This methodology also has the potential to provide a significant source of readily available tissue for IVD degeneration/regeneration studies.
DescriptionPoster Session - Spine: abstract no. 25328
Persistent Identifierhttp://hdl.handle.net/10722/142833

 

DC FieldValueLanguage
dc.contributor.authorYeung, Ken_US
dc.contributor.authorChan, KYen_US
dc.contributor.authorLeung, VYLen_US
dc.contributor.authorTam, Ven_US
dc.contributor.authorLu, WWen_US
dc.contributor.authorSze, KYen_US
dc.contributor.authorCheung, Ken_US
dc.date.accessioned2011-10-28T02:56:32Z-
dc.date.available2011-10-28T02:56:32Z-
dc.date.issued2010en_US
dc.identifier.citationThe 7th SICOT/SIROT Annual International Conference combined meeting with the Swedish Orthopaedic Association (SOF), Gothenburg, Sweden, 31 August-3 September 2010.en_US
dc.identifier.urihttp://hdl.handle.net/10722/142833-
dc.descriptionPoster Session - Spine: abstract no. 25328-
dc.description.abstractStudy of intervertebral disc (IVD) cells in a 3D formation within a niche resembling native extracellular matrix (ECM) is important for researchers to understand the actual cell behaviour and etiology of disc degeneration/regeneration. Removal of resident cells and cellular contents whilst minimizing adverse effects on ECM from IVD is necessary in making a natural scaffold. We hypothesize that bovine discs may be effectively decellularised using a detergent to produce a scaffold resembling native IVD for disc cell investigation. Bovine caudal discs (18-20mm diameter) were harvested and incubated with either 25 mL of PBS containing 0.1% SDS or 1% Triton X-100 (TX-100) in Tris-HCL solution with protease inhibitor. Live/Dead staining and Alamar Blue assay were used to determine numbers of resident cells and their metabolic activity after treatment, respectively.0.1% SDS solution enabled removal of 69% and 73% cell contents from annulus fibrosus and nucleus pulposus respectively, whereas 1% TX-100 removed 54% from both tissues. SDS was the most efficient in removing resident cells from IVD. Being an ionic reagent, SDS has a role in solubilising cytoplasmic and nuclear membranes, which caused the disruption of protein-protein interactions and aided in the clearance of cells. The successful development of a natural scaffold will allow IVD cells to be studied in a physiological 3D microenvironment without the use of in vivo animal models. This methodology also has the potential to provide a significant source of readily available tissue for IVD degeneration/regeneration studies.-
dc.languageengen_US
dc.relation.ispartofSICOT/SIROT Annual International Conferenceen_US
dc.titleEffects of detergents on bovine disc decullularization for the generation of natural disc scaffolds for intervertebral disc tissue engineeringen_US
dc.typeConference_Paperen_US
dc.identifier.emailYeung, K: wkkyeung@hku.hken_US
dc.identifier.emailChan, KY: h0301351@hkusua.hku.hken_US
dc.identifier.emailLeung, VYL: vicleung@hku.hken_US
dc.identifier.emailTam, V: vivtam@hku.hken_US
dc.identifier.emailLu, WW: wwlu@hku.hken_US
dc.identifier.emailSze, KY: kysze@hku.hken_US
dc.identifier.emailCheung, K: cheungmc@hku.hken_US
dc.identifier.authorityYeung, K=rp00309en_US
dc.identifier.authorityLu, WW=rp00411en_US
dc.identifier.authoritySze, KY=rp00171en_US
dc.identifier.authorityCheung, K=rp00387en_US
dc.identifier.hkuros197029en_US
dc.description.otherThe 7th SICOT/SIROT Annual International Conference combined meeting with the Swedish Orthopaedic Association (SOF), Gothenburg, Sweden, 31 August-3 September 2010.-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats