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Conference Paper: Sperm prohibitin expression positively correlates with sperm mitochondrial potential and sperm motility in asthenospermic or oligoasthenospermic subjects

TitleSperm prohibitin expression positively correlates with sperm mitochondrial potential and sperm motility in asthenospermic or oligoasthenospermic subjects
Authors
Issue Date2011
Citation
The 2011 World Congress on Reproductive Biology (WCRB), Cairns, Queensland, Australia, 9-12 October 2011. How to Cite?
AbstractProhibitin is a highly conserved major sperm mitochondrial membrane protein. Based on its roles in the morphogenesis of mitochondrial cristae, in functional subcompartmentalization within mitochondria, and in the respiratory chain, we propose that sperm prohibitin expression may be altered in male infertility associated with poor motility (asthenospermia, A) and/or low sperm concentration (oligoasthenospermia, OA). Semen samples from 68 male subjects between 30-40 years old attempting ICSI/IVF were subjected to sperm motility analysis according to 2010 WHO standards. Sperm mitochondrial potential was quantified by flow cytometry after MitoTracker® Red probe staining. The localization of prohibitin protein in the sperm was detected by immunofluorescence after MitoTracker® Red probe staining while the expression level of prohibitin protein was analyzed using SDS-PAGE and Western blot. The results showed that the prohibitin protein was co-localized with MitoTracker® Red probe in the sperm mitochondria. The sperm mitochondrial potential was significantly lower in the sperm from A (P<0.05) or OA (P<0.001) subjects than normospermia (N) subjects. The decrease in potential was more severe for OA subjects than A subjects (P<0.05). The prohibitin expression was also significantly lower in sperm from A or OA subjects (P<0.05). Positive correlations were found among prohibitin expression, mitochondrial potentials and sperm motility (P<0.05). We conclude that prohibitin may be used as an indicator of sperm quality in asthenospermic or oligoasthenospermic infertile subjects.
DescriptionPosters: Abstract no. 294
Persistent Identifierhttp://hdl.handle.net/10722/142634

 

DC FieldValueLanguage
dc.contributor.authorChen, Hen_US
dc.contributor.authorWang, MJen_US
dc.contributor.authorOU, JXen_US
dc.contributor.authorHuang, SFen_US
dc.contributor.authorChen, GWen_US
dc.contributor.authorO, WSen_US
dc.date.accessioned2011-10-28T02:53:32Z-
dc.date.available2011-10-28T02:53:32Z-
dc.date.issued2011en_US
dc.identifier.citationThe 2011 World Congress on Reproductive Biology (WCRB), Cairns, Queensland, Australia, 9-12 October 2011.en_US
dc.identifier.urihttp://hdl.handle.net/10722/142634-
dc.descriptionPosters: Abstract no. 294-
dc.description.abstractProhibitin is a highly conserved major sperm mitochondrial membrane protein. Based on its roles in the morphogenesis of mitochondrial cristae, in functional subcompartmentalization within mitochondria, and in the respiratory chain, we propose that sperm prohibitin expression may be altered in male infertility associated with poor motility (asthenospermia, A) and/or low sperm concentration (oligoasthenospermia, OA). Semen samples from 68 male subjects between 30-40 years old attempting ICSI/IVF were subjected to sperm motility analysis according to 2010 WHO standards. Sperm mitochondrial potential was quantified by flow cytometry after MitoTracker® Red probe staining. The localization of prohibitin protein in the sperm was detected by immunofluorescence after MitoTracker® Red probe staining while the expression level of prohibitin protein was analyzed using SDS-PAGE and Western blot. The results showed that the prohibitin protein was co-localized with MitoTracker® Red probe in the sperm mitochondria. The sperm mitochondrial potential was significantly lower in the sperm from A (P<0.05) or OA (P<0.001) subjects than normospermia (N) subjects. The decrease in potential was more severe for OA subjects than A subjects (P<0.05). The prohibitin expression was also significantly lower in sperm from A or OA subjects (P<0.05). Positive correlations were found among prohibitin expression, mitochondrial potentials and sperm motility (P<0.05). We conclude that prohibitin may be used as an indicator of sperm quality in asthenospermic or oligoasthenospermic infertile subjects.-
dc.languageengen_US
dc.relation.ispartofWorld Congress on Reproductive Biologyen_US
dc.titleSperm prohibitin expression positively correlates with sperm mitochondrial potential and sperm motility in asthenospermic or oligoasthenospermic subjectsen_US
dc.typeConference_Paperen_US
dc.identifier.emailO, WS: owaisum@hkucc.hku.hken_US
dc.identifier.authorityO, WS=rp00315en_US
dc.identifier.hkuros197684en_US
dc.description.otherThe 2011 World Congress on Reproductive Biology (WCRB), Cairns, Queensland, Australia, 9-12 October 2011.-

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