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Article: ω-3 fatty acids suppress inflammatory cytokine production by macrophages and hepatocytes

Titleω-3 fatty acids suppress inflammatory cytokine production by macrophages and hepatocytes
Authors
Keywordsω-3 fatty acids (EPA)
ω-6 fatty acids (AA)
Inflammation
PNALD
TPN
Issue Date2010
PublisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/jpedsurg
Citation
Journal Of Pediatric Surgery, 2010, v. 45 n. 12, p. 2412-2418 How to Cite?
AbstractObjective: Long-term total parenteral nutrition (TPN) in children is often complicated by parental nutrition-associated liver disease and may even lead to liver failure. Recently, the addition of ω-3 fatty acids to TPN has been shown to reduce the risk of parental nutrition-associated liver disease. The purpose of this study was to explore the anti-inflammatory effects of ω-3 fatty acids (eicosapentaenoic acid [EPA]) to demonstrate the protection of the liver against hepatic steatosis and damage. Materials and Methods: Lipopolysaccharide (LPS) and prostaglandin E 2 (PGE 2) were used to stimulate human macrophages and hepatocytes (THLE-3) to induce in vitro inflammatory condition. The cells were then incubated with either ω-3 (EPA) or ω-6 (arachidonic acid) fatty acids. Supernatants were collected at different time points for the measurement of tumor necrosis factor α (TNF-α), interleukin 6 (IL-6), and interleukin 10 (IL-10) using enzyme-linked immunosorbent assay. Furthermore, pretreated macrophages by LPS stimulation and after incubation with EPA were added to prestimulated hepatocytes for the subsequent measurement of cytokine response. Results: Eicosapentaenoic acid effectively reduced LPS-induced or PGE 2-induced TNF-α and IL-6 expression, and increased IL-10 expression significantly when compared with arachidonic acid. Furthermore, supernatant collected after co-culturing EPA with macrophages also suppressed the levels of TNF-α and IL-6 in hepatocytes. This would suggest that EPA not only had an anti-inflammatory effect on macrophages and hepatocytes directly, it could indirectly reduce hepatocyte inflammation through activated macrophages. Conclusions: The addition of ω-3 fatty acids in TPN suppresses the inflammatory response via direct and indirect routes. The findings may help explain the clinical benefits of EPA in pediatric patients receiving long-term TPN. © 2010 Elsevier Inc.
Persistent Identifierhttp://hdl.handle.net/10722/142520
ISSN
2015 Impact Factor: 1.733
2015 SCImago Journal Rankings: 0.802
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHao, Wen_HK
dc.contributor.authorWong, OYen_HK
dc.contributor.authorLiu, Xen_HK
dc.contributor.authorLee, Pen_HK
dc.contributor.authorChen, Yen_HK
dc.contributor.authorWong, KKYen_HK
dc.date.accessioned2011-10-28T02:50:22Z-
dc.date.available2011-10-28T02:50:22Z-
dc.date.issued2010en_HK
dc.identifier.citationJournal Of Pediatric Surgery, 2010, v. 45 n. 12, p. 2412-2418en_HK
dc.identifier.issn0022-3468en_HK
dc.identifier.urihttp://hdl.handle.net/10722/142520-
dc.description.abstractObjective: Long-term total parenteral nutrition (TPN) in children is often complicated by parental nutrition-associated liver disease and may even lead to liver failure. Recently, the addition of ω-3 fatty acids to TPN has been shown to reduce the risk of parental nutrition-associated liver disease. The purpose of this study was to explore the anti-inflammatory effects of ω-3 fatty acids (eicosapentaenoic acid [EPA]) to demonstrate the protection of the liver against hepatic steatosis and damage. Materials and Methods: Lipopolysaccharide (LPS) and prostaglandin E 2 (PGE 2) were used to stimulate human macrophages and hepatocytes (THLE-3) to induce in vitro inflammatory condition. The cells were then incubated with either ω-3 (EPA) or ω-6 (arachidonic acid) fatty acids. Supernatants were collected at different time points for the measurement of tumor necrosis factor α (TNF-α), interleukin 6 (IL-6), and interleukin 10 (IL-10) using enzyme-linked immunosorbent assay. Furthermore, pretreated macrophages by LPS stimulation and after incubation with EPA were added to prestimulated hepatocytes for the subsequent measurement of cytokine response. Results: Eicosapentaenoic acid effectively reduced LPS-induced or PGE 2-induced TNF-α and IL-6 expression, and increased IL-10 expression significantly when compared with arachidonic acid. Furthermore, supernatant collected after co-culturing EPA with macrophages also suppressed the levels of TNF-α and IL-6 in hepatocytes. This would suggest that EPA not only had an anti-inflammatory effect on macrophages and hepatocytes directly, it could indirectly reduce hepatocyte inflammation through activated macrophages. Conclusions: The addition of ω-3 fatty acids in TPN suppresses the inflammatory response via direct and indirect routes. The findings may help explain the clinical benefits of EPA in pediatric patients receiving long-term TPN. © 2010 Elsevier Inc.en_HK
dc.languageengen_US
dc.publisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/jpedsurgen_HK
dc.relation.ispartofJournal of Pediatric Surgeryen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Journal of Pediatric Surgery. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Pediatric Surgery, 2010, v. 45 n. 12, p. 2412-2418. DOI: 10.1016/j.jpedsurg.2010.08.044-
dc.subjectω-3 fatty acids (EPA)en_HK
dc.subjectω-6 fatty acids (AA)en_HK
dc.subjectInflammationen_HK
dc.subjectPNALDen_HK
dc.subjectTPNen_HK
dc.subject.meshAnti-Inflammatory Agents, Non-Steroidal - pharmacology-
dc.subject.meshEicosapentaenoic Acid - pharmacology-
dc.subject.meshHepatocytes - drug effects - metabolism-
dc.subject.meshInterleukin-10 - biosynthesis - genetics-
dc.subject.meshInterleukin-6 - biosynthesis - genetics-
dc.titleω-3 fatty acids suppress inflammatory cytokine production by macrophages and hepatocytesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-3468&volume=45&issue=12&spage=2412&epage=2418&date=2010&atitle=ω-3+fatty+acids+suppress+inflammatory+cytokine+production+by+macrophages+and+hepatocytes-
dc.identifier.emailChen, Y: ychenc@hkucc.hku.hken_HK
dc.identifier.emailWong, KKY: kkywong@hkucc.hku.hken_HK
dc.identifier.authorityChen, Y=rp01318en_HK
dc.identifier.authorityWong, KKY=rp01392en_HK
dc.description.naturepostprint-
dc.identifier.doi10.1016/j.jpedsurg.2010.08.044en_HK
dc.identifier.pmid21129557-
dc.identifier.scopuseid_2-s2.0-78649746442en_HK
dc.identifier.hkuros184059en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-78649746442&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume45en_HK
dc.identifier.issue12en_HK
dc.identifier.spage2412en_HK
dc.identifier.epage2418en_HK
dc.identifier.isiWOS:000284873300035-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridHao, W=36455883500en_HK
dc.identifier.scopusauthoridWong, OY=37099135600en_HK
dc.identifier.scopusauthoridLiu, X=36106291400en_HK
dc.identifier.scopusauthoridLee, P=8212119000en_HK
dc.identifier.scopusauthoridChen, Y=36463185300en_HK
dc.identifier.scopusauthoridWong, KKY=24438686400en_HK

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