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Article: Invasion of human glioma cells is regulated by multiple chloride channels including ClC-3

TitleInvasion of human glioma cells is regulated by multiple chloride channels including ClC-3
Authors
KeywordsAstrocytoma
Central nervous system
Chlorotoxin
Glioma cells
Invasion
Malignancy
Multiple chloride channels
Issue Date2010
PublisherInternational Institute of Anticancer Research. The Journal's web site is located at http://ar.iiarjournals.org/
Citation
Anticancer Research, 2010, v. 30 n. 11, p. 4515-4524 How to Cite?
AbstractBackground: Glioblastoma is a type of highly malignant primary brain tumour. By means of ion excretion and the associated obligatory water loss, glioma cells can change shapes and undergo extensive migration and invasion. This study investigated the effects of inhibition of ion excretion in glioma cells. Materials and Methods: The expression of chloride channels (ClCs) and metalloproteinase-2 (MMP-2) was studied in two human glioma cell lines (STTG1 and U251-MG). The effects of ClC inhibition with chlorotoxin (a ClC-3 inhibitor), 5-nitro-2-3-phenylpropylamino benzoic acid (NPPB) (a non-specific ClC inhibitor), and ClC-3 siRNA knockdown were studied. Results: Both STTG1 and U251-MG cells expressed ClC family members ClC-2, -3, -4, -5, -6 and -7, as well as MMP-2. Glioma cell invasion was markedly but not completely inhibited by ClC-3 and MMP-2 siRNA knockdown, and by chlorotoxin treatment. Addition of chlorotoxin to siRNA-treated glioma cells only slightly increased the suppression of invasion. In contrast, invasion was completely blocked by the non-specific ClC blocker NPPB. Conclusion: ClCs are crucial in glioma cell migration and invasion. Blockade of a single ClC, however, is not sufficient to achieve complete inhibition of glioma cell invasion, suggesting that any future therapy should be targeted at pharmacological blockade of multiple ClCs.
Persistent Identifierhttp://hdl.handle.net/10722/142515
ISSN
2015 Impact Factor: 1.895
2015 SCImago Journal Rankings: 0.815
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLui, VCHen_HK
dc.contributor.authorLung, SSSen_HK
dc.contributor.authorPu, JKSen_HK
dc.contributor.authorHung, KNen_HK
dc.contributor.authorLeung, GKKen_HK
dc.date.accessioned2011-10-28T02:50:13Z-
dc.date.available2011-10-28T02:50:13Z-
dc.date.issued2010en_HK
dc.identifier.citationAnticancer Research, 2010, v. 30 n. 11, p. 4515-4524en_HK
dc.identifier.issn0250-7005en_HK
dc.identifier.urihttp://hdl.handle.net/10722/142515-
dc.description.abstractBackground: Glioblastoma is a type of highly malignant primary brain tumour. By means of ion excretion and the associated obligatory water loss, glioma cells can change shapes and undergo extensive migration and invasion. This study investigated the effects of inhibition of ion excretion in glioma cells. Materials and Methods: The expression of chloride channels (ClCs) and metalloproteinase-2 (MMP-2) was studied in two human glioma cell lines (STTG1 and U251-MG). The effects of ClC inhibition with chlorotoxin (a ClC-3 inhibitor), 5-nitro-2-3-phenylpropylamino benzoic acid (NPPB) (a non-specific ClC inhibitor), and ClC-3 siRNA knockdown were studied. Results: Both STTG1 and U251-MG cells expressed ClC family members ClC-2, -3, -4, -5, -6 and -7, as well as MMP-2. Glioma cell invasion was markedly but not completely inhibited by ClC-3 and MMP-2 siRNA knockdown, and by chlorotoxin treatment. Addition of chlorotoxin to siRNA-treated glioma cells only slightly increased the suppression of invasion. In contrast, invasion was completely blocked by the non-specific ClC blocker NPPB. Conclusion: ClCs are crucial in glioma cell migration and invasion. Blockade of a single ClC, however, is not sufficient to achieve complete inhibition of glioma cell invasion, suggesting that any future therapy should be targeted at pharmacological blockade of multiple ClCs.en_HK
dc.languageengen_US
dc.publisherInternational Institute of Anticancer Research. The Journal's web site is located at http://ar.iiarjournals.org/en_HK
dc.relation.ispartofAnticancer Researchen_HK
dc.subjectAstrocytomaen_HK
dc.subjectCentral nervous systemen_HK
dc.subjectChlorotoxinen_HK
dc.subjectGlioma cellsen_HK
dc.subjectInvasionen_HK
dc.subjectMalignancyen_HK
dc.subjectMultiple chloride channelsen_HK
dc.subject.meshBrain Neoplasms - genetics - pathology-
dc.subject.meshCell Movement - drug effects-
dc.subject.meshChloride Channels - physiology-
dc.subject.meshGlioma - genetics - pathology-
dc.subject.meshMatrix Metalloproteinase 2 - physiology-
dc.titleInvasion of human glioma cells is regulated by multiple chloride channels including ClC-3en_HK
dc.typeArticleen_HK
dc.identifier.emailLui, VCH: vchlui@hkucc.hku.hken_HK
dc.identifier.emailLeung, GKK: gilberto@hkucc.hku.hken_HK
dc.identifier.authorityLui, VCH=rp00363en_HK
dc.identifier.authorityLeung, GKK=rp00522en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid21115901-
dc.identifier.scopuseid_2-s2.0-78650230042en_HK
dc.identifier.hkuros183879en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-78650230042&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume30en_HK
dc.identifier.issue11en_HK
dc.identifier.spage4515en_HK
dc.identifier.epage4524en_HK
dc.identifier.isiWOS:000285237100018-
dc.publisher.placeGreeceen_HK
dc.identifier.scopusauthoridLui, VCH=7004231344en_HK
dc.identifier.scopusauthoridLung, SSS=37031564300en_HK
dc.identifier.scopusauthoridPu, JKS=35094475800en_HK
dc.identifier.scopusauthoridHung, KN=7202728375en_HK
dc.identifier.scopusauthoridLeung, GKK=35965118200en_HK

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