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Article: Osteoprotegerin deficiency attenuates strontium-mediated inhibition of osteoclastogenesis and bone resorption

TitleOsteoprotegerin deficiency attenuates strontium-mediated inhibition of osteoclastogenesis and bone resorption
Authors
KeywordsBone Formation
Bone Resorption
Osteoporosis
Osteoprotegerin
Strontium
Issue Date2011
PublisherAmerican Society for Bone and Mineral Research. The Journal's web site is located at http://www.jbmr.org/view/0/index.html
Citation
Journal Of Bone And Mineral Research, 2011, v. 26 n. 6, p. 1272-1282 How to Cite?
AbstractStrontium (Sr) exerts an anabolic and antiresorptive effect on bone, but the mechanism remains unknown. Osteoprotegerin (OPG) expressed by osteoblasts plays an important role in regulating bone homeostasis by inhibiting osteoclastogenesis and bone resorption. This study aims at evaluating the role of OPG in Sr-mediated inhibition of osteoclastogenesis and bone resorption. Six-week-old Opg knockout (KO) male mice and their wild-type (WT) littermates were treated orally with vehicle (Veh) or Sr compound (4 mmol/kg) daily for 8 weeks. Bone mass and microstructure in the lumbar spine (L 4) and proximal tibia were analyzed with micro-computed tomography (μCT). Bone remodeling was evaluated with serum biochemical analysis and static and dynamic bone histomorphometry. Osteoclast differentiation potential and gene expression were analyzed in bone marrow cells. The findings demonstrate that Sr compound treatment results in greater bone volume and trabecular number than Veh treatment in WT mice. The anabolic response of trabecular bone to Sr treatment is attenuated in KO mice. Although Sr treatment significantly decreases in vitro osteoclastogenesis and bone resorption in WT mice, these effects are attenuated in KO mice. Furthermore, Sr treatment profoundly increases Opg gene expression in the tibias and OPG protein levels in the sera of WT mice. This study concludes that the inhibition of osteoclastogenesis and bone resorption is possibly associated with OPG upregulation by Sr treatment. © 2011 American Society for Bone and Mineral Research. Copyright © 2011 American Society for Bone and Mineral Research.
Persistent Identifierhttp://hdl.handle.net/10722/142441
ISSN
2015 Impact Factor: 5.622
2015 SCImago Journal Rankings: 2.773
ISI Accession Number ID
Funding AgencyGrant Number
Innovation and Technology Fund (ITF)GHP/009/06
Hong Kong Research Grants Council (RGC)HKU7147/07
Funding Information:

We would like to thank Dr Dong Zheng for his careful animal handling in this study. This study was supported by the Innovation and Technology Fund (ITF; Project Ref. No. GHP/009/06) and Hong Kong Research Grants Council (RGC) Grant HKU7147/07.

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorPeng, Sen_HK
dc.contributor.authorLiu, XSen_HK
dc.contributor.authorZhou, Gen_HK
dc.contributor.authorLi, Zen_HK
dc.contributor.authorLuk, KDKen_HK
dc.contributor.authorGuo, XEen_HK
dc.contributor.authorLu, WWen_HK
dc.date.accessioned2011-10-28T02:46:05Z-
dc.date.available2011-10-28T02:46:05Z-
dc.date.issued2011en_HK
dc.identifier.citationJournal Of Bone And Mineral Research, 2011, v. 26 n. 6, p. 1272-1282en_HK
dc.identifier.issn0884-0431en_HK
dc.identifier.urihttp://hdl.handle.net/10722/142441-
dc.description.abstractStrontium (Sr) exerts an anabolic and antiresorptive effect on bone, but the mechanism remains unknown. Osteoprotegerin (OPG) expressed by osteoblasts plays an important role in regulating bone homeostasis by inhibiting osteoclastogenesis and bone resorption. This study aims at evaluating the role of OPG in Sr-mediated inhibition of osteoclastogenesis and bone resorption. Six-week-old Opg knockout (KO) male mice and their wild-type (WT) littermates were treated orally with vehicle (Veh) or Sr compound (4 mmol/kg) daily for 8 weeks. Bone mass and microstructure in the lumbar spine (L 4) and proximal tibia were analyzed with micro-computed tomography (μCT). Bone remodeling was evaluated with serum biochemical analysis and static and dynamic bone histomorphometry. Osteoclast differentiation potential and gene expression were analyzed in bone marrow cells. The findings demonstrate that Sr compound treatment results in greater bone volume and trabecular number than Veh treatment in WT mice. The anabolic response of trabecular bone to Sr treatment is attenuated in KO mice. Although Sr treatment significantly decreases in vitro osteoclastogenesis and bone resorption in WT mice, these effects are attenuated in KO mice. Furthermore, Sr treatment profoundly increases Opg gene expression in the tibias and OPG protein levels in the sera of WT mice. This study concludes that the inhibition of osteoclastogenesis and bone resorption is possibly associated with OPG upregulation by Sr treatment. © 2011 American Society for Bone and Mineral Research. Copyright © 2011 American Society for Bone and Mineral Research.en_HK
dc.languageengen_US
dc.publisherAmerican Society for Bone and Mineral Research. The Journal's web site is located at http://www.jbmr.org/view/0/index.htmlen_HK
dc.relation.ispartofJournal of Bone and Mineral Researchen_HK
dc.subjectBone Formationen_HK
dc.subjectBone Resorptionen_HK
dc.subjectOsteoporosisen_HK
dc.subjectOsteoprotegerinen_HK
dc.subjectStrontiumen_HK
dc.titleOsteoprotegerin deficiency attenuates strontium-mediated inhibition of osteoclastogenesis and bone resorptionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0884-0431&volume=26&issue=6&spage=1272&epage=1282&date=2011&atitle=Osteoprotegerin+deficiency+attenuates+strontium-mediated+inhibition+of+osteoclastogenesis+and+bone+resorptionen_US
dc.identifier.emailZhou, G:wormoscz@gmail.comen_HK
dc.identifier.emailLuk, KDK:hcm21000@hku.hken_HK
dc.identifier.emailLu, WW:wwlu@hku.hken_HK
dc.identifier.authorityZhou, G=rp00527en_HK
dc.identifier.authorityLuk, KDK=rp00333en_HK
dc.identifier.authorityLu, WW=rp00411en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1002/jbmr.325en_HK
dc.identifier.pmid21611968-
dc.identifier.scopuseid_2-s2.0-79958773712en_HK
dc.identifier.hkuros196997en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79958773712&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume26en_HK
dc.identifier.issue6en_HK
dc.identifier.spage1272en_HK
dc.identifier.epage1282en_HK
dc.identifier.eissn1523-4681-
dc.identifier.isiWOS:000291109100013-
dc.publisher.placeUnited Statesen_HK
dc.relation.projectOptimization and commercialization of strontium containing bioactive bone cement for various orthopaedic applications-
dc.identifier.scopusauthoridPeng, S=13402746900en_HK
dc.identifier.scopusauthoridLiu, XS=50061438600en_HK
dc.identifier.scopusauthoridZhou, G=23394245100en_HK
dc.identifier.scopusauthoridLi, Z=35784563200en_HK
dc.identifier.scopusauthoridLuk, KDK=7201921573en_HK
dc.identifier.scopusauthoridGuo, XE=35237105200en_HK
dc.identifier.scopusauthoridLu, WW=7404215221en_HK

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