File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: GC/MS-based metabolomics reveals fatty acid biosynthesis and cholesterol metabolism in cell lines infected with influenza A virus

TitleGC/MS-based metabolomics reveals fatty acid biosynthesis and cholesterol metabolism in cell lines infected with influenza A virus
Authors
KeywordsGC/MS
Influenza A virus
Metabolomics
Multivariate data analysis
Issue Date2010
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/talanta
Citation
Talanta, 2010, v. 83 n. 1, p. 262-268 How to Cite?
AbstractMetabolomics is the downstream of systems biology and has drawn significant interest for studying the metabolic networks from cells to organisms. To profile the metabolites in two different cell lines (A549 and AGS) infected with influenza A virus, gas chromatography coupled with mass spectrometry (GC/MS) was employed. Some differentiating metabolites in the cell lines were tentatively identified using reference library, interpreted and visualized by applying principal components analysis (PCA) and cluster heat map. Consequently, metabolic flux profiling allowed the differentiation of fatty acid biosynthesis and cholesterol metabolism during viral replication in the cell lines. The change in fatty acid turnover was also observed. Metabolomics investigation also revealed the different responses between A549 and AGS cell lines to the virus infection. From the pattern recognition results, AGS cell line might be more susceptible to influenza A virus. Regarding the fact that AGS is a poorly differentiated gastric adenocarcinoma cell line whereas A549 is a relatively differentiated lung tumor one, it is speculated that viral replication might be associated with the cell differentiations. © 2010 Elsevier B.V.
Persistent Identifierhttp://hdl.handle.net/10722/142421
ISSN
2015 Impact Factor: 4.035
2015 SCImago Journal Rankings: 1.233
ISI Accession Number ID
Funding AgencyGrant Number
National Nature Sciences Foundation of China20928005
Funding Information:

The authors would like to thank the National Nature Sciences Foundation of China (20928005) for the financial support

References

 

DC FieldValueLanguage
dc.contributor.authorLin, Sen_HK
dc.contributor.authorLiu, Nen_HK
dc.contributor.authorYang, Zen_HK
dc.contributor.authorSong, Wen_HK
dc.contributor.authorWang, Pen_HK
dc.contributor.authorChen, Hen_HK
dc.contributor.authorLucio, Men_HK
dc.contributor.authorSchmittKopplin, Pen_HK
dc.contributor.authorChen, Gen_HK
dc.contributor.authorCai, Zen_HK
dc.date.accessioned2011-10-28T02:45:40Z-
dc.date.available2011-10-28T02:45:40Z-
dc.date.issued2010en_HK
dc.identifier.citationTalanta, 2010, v. 83 n. 1, p. 262-268en_HK
dc.identifier.issn0039-9140en_HK
dc.identifier.urihttp://hdl.handle.net/10722/142421-
dc.description.abstractMetabolomics is the downstream of systems biology and has drawn significant interest for studying the metabolic networks from cells to organisms. To profile the metabolites in two different cell lines (A549 and AGS) infected with influenza A virus, gas chromatography coupled with mass spectrometry (GC/MS) was employed. Some differentiating metabolites in the cell lines were tentatively identified using reference library, interpreted and visualized by applying principal components analysis (PCA) and cluster heat map. Consequently, metabolic flux profiling allowed the differentiation of fatty acid biosynthesis and cholesterol metabolism during viral replication in the cell lines. The change in fatty acid turnover was also observed. Metabolomics investigation also revealed the different responses between A549 and AGS cell lines to the virus infection. From the pattern recognition results, AGS cell line might be more susceptible to influenza A virus. Regarding the fact that AGS is a poorly differentiated gastric adenocarcinoma cell line whereas A549 is a relatively differentiated lung tumor one, it is speculated that viral replication might be associated with the cell differentiations. © 2010 Elsevier B.V.en_HK
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/talantaen_HK
dc.relation.ispartofTalantaen_HK
dc.subjectGC/MSen_HK
dc.subjectInfluenza A virusen_HK
dc.subjectMetabolomicsen_HK
dc.subjectMultivariate data analysisen_HK
dc.subject.meshCholesterol - metabolism-
dc.subject.meshFatty Acids - metabolism-
dc.subject.meshHost-Pathogen Interactions-
dc.subject.meshInfluenza A virus - physiology-
dc.subject.meshInfluenza, Human - metabolism-
dc.titleGC/MS-based metabolomics reveals fatty acid biosynthesis and cholesterol metabolism in cell lines infected with influenza A virusen_HK
dc.typeArticleen_HK
dc.identifier.emailChen, H:hlchen@hkucc.hku.hken_HK
dc.identifier.authorityChen, H=rp00383en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.talanta.2010.09.019en_HK
dc.identifier.pmid21035673-
dc.identifier.scopuseid_2-s2.0-78049365059en_HK
dc.identifier.hkuros197237en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-78049365059&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume83en_HK
dc.identifier.issue1en_HK
dc.identifier.spage262en_HK
dc.identifier.epage268en_HK
dc.identifier.isiWOS:000284972800039-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridLin, S=36237034900en_HK
dc.identifier.scopusauthoridLiu, N=36079934800en_HK
dc.identifier.scopusauthoridYang, Z=7405430792en_HK
dc.identifier.scopusauthoridSong, W=23490429800en_HK
dc.identifier.scopusauthoridWang, P=13907209900en_HK
dc.identifier.scopusauthoridChen, H=26643315400en_HK
dc.identifier.scopusauthoridLucio, M=23967631600en_HK
dc.identifier.scopusauthoridSchmittKopplin, P=35499111100en_HK
dc.identifier.scopusauthoridChen, G=34869192200en_HK
dc.identifier.scopusauthoridCai, Z=7402904946en_HK
dc.identifier.citeulike7921495-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats