File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Differential diagnosis of pandemic (H1N1) 2009 infection by detection of haemagglutinin with an enzyme-linked immunoassay

TitleDifferential diagnosis of pandemic (H1N1) 2009 infection by detection of haemagglutinin with an enzyme-linked immunoassay
Authors
KeywordsHaemagglutinin
Immunoassay
Influenza A virus
Influenza diagnosis
Pandemic (H1N1) 2009 virus
Issue Date2011
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CLM
Citation
Clinical Microbiology And Infection, 2011, v. 17 n. 10, p. 1574-1580 How to Cite?
Abstract
A sensitive and convenient immunoassay that can directly differentiate pandemic (H1N1) 2009 (pH1N1) virus from seasonal influenza virus can play an important role in the clinic. In the presented study, a double-sandwich ELISA (pH1N1 ELISA), based on two monoclonal antibodies against haemagglutinin (HA) of the pH1N1 virus, was developed. After laboratory determination of the sensitivity and specificity characteristics, the performance of this assay was evaluated in a cohort of 904 patients with influenza-like illness. All seven strains of pH1N1 virus tested were positive by pH1N1 ELISA, with an average lower detection limit of 10 3.0±0.4 tissue culture infective dose (TCID) 50/mL (or 0.009±0.005HA titre). Cross-reaction of the assay with seasonal influenza virus and other common respiratory pathogens was rare. In pH1N1-infected patients, the sensitivity of the pH1N1 ELISA was 92.3% (84/91, 95% CI 84.8-96.9%), which is significantly higher than that of the BD Directigen EZ Flu A+B test (70.3%, p<0.01). The specificity of pH1N1 ELISA in seasonal influenza A patients was 100.0% (171/171, 95% CI 97.9-100.0%), similar to that in non-influenza A patients (640/642, 99.7%, 95% CI 98.9-100.0%). The positive predictive value for pH1N1 ELISA was 97.7% and the negative predictive value was 99.1% in this study population with a pH1N1 prevalence of 10.1%. In conclusion, detection of HA of pH1N1 virus by immunoassay appears to be a convenient and reliable method for the differential diagnosis of pH1N1 from other respiratory pathogens, including seasonal influenza virus. © 2011 The Authors. Clinical Microbiology and Infection © 2011 European Society of Clinical Microbiology and Infectious Diseases.
Persistent Identifierhttp://hdl.handle.net/10722/142420
ISSN
2013 Impact Factor: 5.197
2013 SCImago Journal Rankings: 2.280
ISI Accession Number ID
Funding AgencyGrant Number
Key Special Subjects of Infectious Diseases2008ZX10004-006
Key Project of Science & Technology of Fujian Province2008Y0059
National High Technology Research and Development Program2010AA022801
Funding Information:

This work was supported by grants from Key Special Subjects of Infectious Diseases (2008ZX10004-006), the Key Project of Science & Technology of Fujian Province (2008Y0059) and the National High Technology Research and Development Program (2010AA022801).

References

 

Author Affiliations
  1. Xijing Hospital
  2. Xiamen University
  3. The University of Hong Kong
DC FieldValueLanguage
dc.contributor.authorYuan, Qen_HK
dc.contributor.authorCheng, XDen_HK
dc.contributor.authorYang, BCen_HK
dc.contributor.authorZheng, QBen_HK
dc.contributor.authorChen, YXen_HK
dc.contributor.authorChen, QRen_HK
dc.contributor.authorZeng, Fen_HK
dc.contributor.authorZhang, Ren_HK
dc.contributor.authorGe, SXen_HK
dc.contributor.authorHao, XKen_HK
dc.contributor.authorChen, Hen_HK
dc.contributor.authorZhang, Jen_HK
dc.contributor.authorXia, NSen_HK
dc.date.accessioned2011-10-28T02:45:38Z-
dc.date.available2011-10-28T02:45:38Z-
dc.date.issued2011en_HK
dc.identifier.citationClinical Microbiology And Infection, 2011, v. 17 n. 10, p. 1574-1580en_HK
dc.identifier.issn1198-743Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/142420-
dc.description.abstractA sensitive and convenient immunoassay that can directly differentiate pandemic (H1N1) 2009 (pH1N1) virus from seasonal influenza virus can play an important role in the clinic. In the presented study, a double-sandwich ELISA (pH1N1 ELISA), based on two monoclonal antibodies against haemagglutinin (HA) of the pH1N1 virus, was developed. After laboratory determination of the sensitivity and specificity characteristics, the performance of this assay was evaluated in a cohort of 904 patients with influenza-like illness. All seven strains of pH1N1 virus tested were positive by pH1N1 ELISA, with an average lower detection limit of 10 3.0±0.4 tissue culture infective dose (TCID) 50/mL (or 0.009±0.005HA titre). Cross-reaction of the assay with seasonal influenza virus and other common respiratory pathogens was rare. In pH1N1-infected patients, the sensitivity of the pH1N1 ELISA was 92.3% (84/91, 95% CI 84.8-96.9%), which is significantly higher than that of the BD Directigen EZ Flu A+B test (70.3%, p<0.01). The specificity of pH1N1 ELISA in seasonal influenza A patients was 100.0% (171/171, 95% CI 97.9-100.0%), similar to that in non-influenza A patients (640/642, 99.7%, 95% CI 98.9-100.0%). The positive predictive value for pH1N1 ELISA was 97.7% and the negative predictive value was 99.1% in this study population with a pH1N1 prevalence of 10.1%. In conclusion, detection of HA of pH1N1 virus by immunoassay appears to be a convenient and reliable method for the differential diagnosis of pH1N1 from other respiratory pathogens, including seasonal influenza virus. © 2011 The Authors. Clinical Microbiology and Infection © 2011 European Society of Clinical Microbiology and Infectious Diseases.en_HK
dc.languageengen_US
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CLMen_HK
dc.relation.ispartofClinical Microbiology and Infectionen_HK
dc.rightsThe definitive version is available at www.blackwell-synergy.com-
dc.subjectHaemagglutininen_HK
dc.subjectImmunoassayen_HK
dc.subjectInfluenza A virusen_HK
dc.subjectInfluenza diagnosisen_HK
dc.subjectPandemic (H1N1) 2009 virusen_HK
dc.subject.meshAntibodies, Viral - immunology-
dc.subject.meshHemagglutinin Glycoproteins, Influenza Virus - diagnostic use - immunology-
dc.subject.meshInfluenza A Virus, H1N1 Subtype - immunology - pathogenicity-
dc.subject.meshInfluenza, Human - diagnosis - epidemiology - immunology - virology-
dc.subject.meshPandemics-
dc.titleDifferential diagnosis of pandemic (H1N1) 2009 infection by detection of haemagglutinin with an enzyme-linked immunoassayen_HK
dc.typeArticleen_HK
dc.identifier.emailChen, H:hlchen@hkucc.hku.hken_HK
dc.identifier.authorityChen, H=rp00383en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1111/j.1469-0691.2010.03413.xen_HK
dc.identifier.pmid21054661en_HK
dc.identifier.scopuseid_2-s2.0-79959740973en_HK
dc.identifier.hkuros197236en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79959740973&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume17en_HK
dc.identifier.issue10en_HK
dc.identifier.spage1574en_HK
dc.identifier.epage1580en_HK
dc.identifier.isiWOS:000295086100023-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridYuan, Q=36060907700en_HK
dc.identifier.scopusauthoridCheng, XD=48360950000en_HK
dc.identifier.scopusauthoridYang, BC=16641545000en_HK
dc.identifier.scopusauthoridZheng, QB=35202845100en_HK
dc.identifier.scopusauthoridChen, YX=50261246900en_HK
dc.identifier.scopusauthoridChen, QR=35085868700en_HK
dc.identifier.scopusauthoridZeng, F=50263511100en_HK
dc.identifier.scopusauthoridZhang, R=7404865007en_HK
dc.identifier.scopusauthoridGe, SX=35227303000en_HK
dc.identifier.scopusauthoridHao, XK=13607007900en_HK
dc.identifier.scopusauthoridChen, H=26643315400en_HK
dc.identifier.scopusauthoridZhang, J=50263742100en_HK
dc.identifier.scopusauthoridXia, NS=35187953700en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats