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Article: Differences in antibody responses of individuals with natural infection and those vaccinated against pandemic H1N1 2009 influenza
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TitleDifferences in antibody responses of individuals with natural infection and those vaccinated against pandemic H1N1 2009 influenza
 
AuthorsChan, KH1
To, KKW1
Hung, IFN1
Zhang, AJX1
Chan, JFW1
Cheng, VCC1
Tse, H1
Che, XY2
Chen, H1
Yuen, KY1
 
Issue Date2011
 
PublisherAmerican Society for Microbiology. The Journal's web site is located at http://cdli.asm.org/
 
CitationClinical And Vaccine Immunology, 2011, v. 18 n. 5, p. 867-873 [How to Cite?]
DOI: http://dx.doi.org/10.1128/CVI.00555-10
 
AbstractThe differential antibody response measured by the commonly used hemagglutination inhibition (HI) and microneutralization (MN) assays in patients with natural infection and vaccination has not been fully assessed. HI and conventional MN (CMN) assays were performed on sera from 651 patients with natural infection by pandemic H1N1 2009 influenza virus and on sera from 567 recipients of the corresponding vaccine. Surprisingly, the overall seroprotection rates determined by CMN and HI assays in vaccine recipients were only 44.8 and 35.1%, respectively. Antibody titers measured by the CMN assay was significantly higher than that obtained by HI assay in vaccine recipients aged ≥50 years, but these titers were not significantly different among younger vaccine recipients. In contrast, the HI titer was greater than the CMN titer for the age group from 16 to 29 years but was not significantly different in other age groups for natural infection. Lower antibody levels were found in both naturally infected patients and immunized recipients in the older than in the younger age groups, but naturally infected patients exhibited higher HI and CMN titers than did the corresponding vaccine recipients. In addition, we developed a rapid fluorescent focus microneutralization (FFMN) assay to test sera from naturally infected patients. The FFMN assay has a better correlation with CMN than with HI (ρ = 0.810 versus 0.684), which is expected of neutralizing antibody mainly targeted toward the inhibition of viral entry into cells. The higher antibody level elicited by natural infection than by vaccination may be related to differences between antigen presentation by the intramuscular route of vaccination and mucosal viral replication in mucosal cells of the respiratory tract. Copyright © 2011, American Society for Microbiology. All Rights Reserved.
 
ISSN1556-6811
2012 Impact Factor: 2.598
2012 SCImago Journal Rankings: 0.938
 
DOIhttp://dx.doi.org/10.1128/CVI.00555-10
 
PubMed Central IDPMC3122523
 
ISI Accession Number IDWOS:000290156600024
Funding AgencyGrant Number
National Science and Technology Major Project of China2009ZX10004-306
Ted Sun Foundation
Clinical Infectious Diseases Research Endowment Fund from Teresa Wong On Yik
Research Fund for the Control of Infectious Diseases of the Food and Health Bureau
Hong Kong Special Administrative Region, China
Funding Information:

We are grateful for the support of the National Science and Technology Major Project of China (grant 2009ZX10004-306), the Ted Sun Foundation, the Clinical Infectious Diseases Research Endowment Fund from Teresa Wong On Yik, the Research Fund for the Control of Infectious Diseases of the Food and Health Bureau, and the Research Grants Council of the Hong Kong Special Administrative Region, China.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorChan, KH
 
dc.contributor.authorTo, KKW
 
dc.contributor.authorHung, IFN
 
dc.contributor.authorZhang, AJX
 
dc.contributor.authorChan, JFW
 
dc.contributor.authorCheng, VCC
 
dc.contributor.authorTse, H
 
dc.contributor.authorChe, XY
 
dc.contributor.authorChen, H
 
dc.contributor.authorYuen, KY
 
dc.date.accessioned2011-10-28T02:45:36Z
 
dc.date.available2011-10-28T02:45:36Z
 
dc.date.issued2011
 
dc.description.abstractThe differential antibody response measured by the commonly used hemagglutination inhibition (HI) and microneutralization (MN) assays in patients with natural infection and vaccination has not been fully assessed. HI and conventional MN (CMN) assays were performed on sera from 651 patients with natural infection by pandemic H1N1 2009 influenza virus and on sera from 567 recipients of the corresponding vaccine. Surprisingly, the overall seroprotection rates determined by CMN and HI assays in vaccine recipients were only 44.8 and 35.1%, respectively. Antibody titers measured by the CMN assay was significantly higher than that obtained by HI assay in vaccine recipients aged ≥50 years, but these titers were not significantly different among younger vaccine recipients. In contrast, the HI titer was greater than the CMN titer for the age group from 16 to 29 years but was not significantly different in other age groups for natural infection. Lower antibody levels were found in both naturally infected patients and immunized recipients in the older than in the younger age groups, but naturally infected patients exhibited higher HI and CMN titers than did the corresponding vaccine recipients. In addition, we developed a rapid fluorescent focus microneutralization (FFMN) assay to test sera from naturally infected patients. The FFMN assay has a better correlation with CMN than with HI (ρ = 0.810 versus 0.684), which is expected of neutralizing antibody mainly targeted toward the inhibition of viral entry into cells. The higher antibody level elicited by natural infection than by vaccination may be related to differences between antigen presentation by the intramuscular route of vaccination and mucosal viral replication in mucosal cells of the respiratory tract. Copyright © 2011, American Society for Microbiology. All Rights Reserved.
 
dc.description.naturelink_to_OA_fulltext
 
dc.identifier.citationClinical And Vaccine Immunology, 2011, v. 18 n. 5, p. 867-873 [How to Cite?]
DOI: http://dx.doi.org/10.1128/CVI.00555-10
 
dc.identifier.doihttp://dx.doi.org/10.1128/CVI.00555-10
 
dc.identifier.epage873
 
dc.identifier.hkuros197223
 
dc.identifier.hkuros190824
 
dc.identifier.isiWOS:000290156600024
Funding AgencyGrant Number
National Science and Technology Major Project of China2009ZX10004-306
Ted Sun Foundation
Clinical Infectious Diseases Research Endowment Fund from Teresa Wong On Yik
Research Fund for the Control of Infectious Diseases of the Food and Health Bureau
Hong Kong Special Administrative Region, China
Funding Information:

We are grateful for the support of the National Science and Technology Major Project of China (grant 2009ZX10004-306), the Ted Sun Foundation, the Clinical Infectious Diseases Research Endowment Fund from Teresa Wong On Yik, the Research Fund for the Control of Infectious Diseases of the Food and Health Bureau, and the Research Grants Council of the Hong Kong Special Administrative Region, China.

 
dc.identifier.issn1556-6811
2012 Impact Factor: 2.598
2012 SCImago Journal Rankings: 0.938
 
dc.identifier.issue5
 
dc.identifier.openurl
 
dc.identifier.pmcidPMC3122523
 
dc.identifier.pmid21411604
 
dc.identifier.scopuseid_2-s2.0-79955707809
 
dc.identifier.spage867
 
dc.identifier.urihttp://hdl.handle.net/10722/142418
 
dc.identifier.volume18
 
dc.languageeng
 
dc.publisherAmerican Society for Microbiology. The Journal's web site is located at http://cdli.asm.org/
 
dc.publisher.placeUnited States
 
dc.relation.ispartofClinical and Vaccine Immunology
 
dc.relation.referencesReferences in Scopus
 
dc.rightsClinical and Vaccine Immunology. Copyright © American Society for Microbiology.
 
dc.subject.meshAntibody Formation
 
dc.subject.meshHemagglutination Inhibition Tests
 
dc.subject.meshInfluenza A Virus, H1N1 Subtype - immunology
 
dc.subject.meshInfluenza Vaccines - immunology
 
dc.subject.meshInfluenza, Human - immunology
 
dc.titleDifferences in antibody responses of individuals with natural infection and those vaccinated against pandemic H1N1 2009 influenza
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong
  2. Zhujiang Hospital