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Article: Differences in antibody responses of individuals with natural infection and those vaccinated against pandemic H1N1 2009 influenza

TitleDifferences in antibody responses of individuals with natural infection and those vaccinated against pandemic H1N1 2009 influenza
Authors
Issue Date2011
PublisherAmerican Society for Microbiology. The Journal's web site is located at http://cdli.asm.org/
Citation
Clinical And Vaccine Immunology, 2011, v. 18 n. 5, p. 867-873 How to Cite?
AbstractThe differential antibody response measured by the commonly used hemagglutination inhibition (HI) and microneutralization (MN) assays in patients with natural infection and vaccination has not been fully assessed. HI and conventional MN (CMN) assays were performed on sera from 651 patients with natural infection by pandemic H1N1 2009 influenza virus and on sera from 567 recipients of the corresponding vaccine. Surprisingly, the overall seroprotection rates determined by CMN and HI assays in vaccine recipients were only 44.8 and 35.1%, respectively. Antibody titers measured by the CMN assay was significantly higher than that obtained by HI assay in vaccine recipients aged ≥50 years, but these titers were not significantly different among younger vaccine recipients. In contrast, the HI titer was greater than the CMN titer for the age group from 16 to 29 years but was not significantly different in other age groups for natural infection. Lower antibody levels were found in both naturally infected patients and immunized recipients in the older than in the younger age groups, but naturally infected patients exhibited higher HI and CMN titers than did the corresponding vaccine recipients. In addition, we developed a rapid fluorescent focus microneutralization (FFMN) assay to test sera from naturally infected patients. The FFMN assay has a better correlation with CMN than with HI (ρ = 0.810 versus 0.684), which is expected of neutralizing antibody mainly targeted toward the inhibition of viral entry into cells. The higher antibody level elicited by natural infection than by vaccination may be related to differences between antigen presentation by the intramuscular route of vaccination and mucosal viral replication in mucosal cells of the respiratory tract. Copyright © 2011, American Society for Microbiology. All Rights Reserved.
Persistent Identifierhttp://hdl.handle.net/10722/142418
ISSN
2014 Impact Factor: 2.470
2014 SCImago Journal Rankings: 1.157
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
National Science and Technology Major Project of China2009ZX10004-306
Ted Sun Foundation
Clinical Infectious Diseases Research Endowment Fund from Teresa Wong On Yik
Research Fund for the Control of Infectious Diseases of the Food and Health Bureau
Hong Kong Special Administrative Region, China
Funding Information:

We are grateful for the support of the National Science and Technology Major Project of China (grant 2009ZX10004-306), the Ted Sun Foundation, the Clinical Infectious Diseases Research Endowment Fund from Teresa Wong On Yik, the Research Fund for the Control of Infectious Diseases of the Food and Health Bureau, and the Research Grants Council of the Hong Kong Special Administrative Region, China.

References

 

DC FieldValueLanguage
dc.contributor.authorChan, KHen_HK
dc.contributor.authorTo, KKWen_HK
dc.contributor.authorHung, IFNen_HK
dc.contributor.authorZhang, AJXen_HK
dc.contributor.authorChan, JFWen_HK
dc.contributor.authorCheng, VCCen_HK
dc.contributor.authorTse, Hen_HK
dc.contributor.authorChe, XYen_HK
dc.contributor.authorChen, Hen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2011-10-28T02:45:36Z-
dc.date.available2011-10-28T02:45:36Z-
dc.date.issued2011en_HK
dc.identifier.citationClinical And Vaccine Immunology, 2011, v. 18 n. 5, p. 867-873en_HK
dc.identifier.issn1556-6811en_HK
dc.identifier.urihttp://hdl.handle.net/10722/142418-
dc.description.abstractThe differential antibody response measured by the commonly used hemagglutination inhibition (HI) and microneutralization (MN) assays in patients with natural infection and vaccination has not been fully assessed. HI and conventional MN (CMN) assays were performed on sera from 651 patients with natural infection by pandemic H1N1 2009 influenza virus and on sera from 567 recipients of the corresponding vaccine. Surprisingly, the overall seroprotection rates determined by CMN and HI assays in vaccine recipients were only 44.8 and 35.1%, respectively. Antibody titers measured by the CMN assay was significantly higher than that obtained by HI assay in vaccine recipients aged ≥50 years, but these titers were not significantly different among younger vaccine recipients. In contrast, the HI titer was greater than the CMN titer for the age group from 16 to 29 years but was not significantly different in other age groups for natural infection. Lower antibody levels were found in both naturally infected patients and immunized recipients in the older than in the younger age groups, but naturally infected patients exhibited higher HI and CMN titers than did the corresponding vaccine recipients. In addition, we developed a rapid fluorescent focus microneutralization (FFMN) assay to test sera from naturally infected patients. The FFMN assay has a better correlation with CMN than with HI (ρ = 0.810 versus 0.684), which is expected of neutralizing antibody mainly targeted toward the inhibition of viral entry into cells. The higher antibody level elicited by natural infection than by vaccination may be related to differences between antigen presentation by the intramuscular route of vaccination and mucosal viral replication in mucosal cells of the respiratory tract. Copyright © 2011, American Society for Microbiology. All Rights Reserved.en_HK
dc.languageengen_US
dc.publisherAmerican Society for Microbiology. The Journal's web site is located at http://cdli.asm.org/en_HK
dc.relation.ispartofClinical and Vaccine Immunologyen_HK
dc.rightsClinical and Vaccine Immunology. Copyright © American Society for Microbiology.-
dc.subject.meshAntibody Formation-
dc.subject.meshHemagglutination Inhibition Tests-
dc.subject.meshInfluenza A Virus, H1N1 Subtype - immunology-
dc.subject.meshInfluenza Vaccines - immunology-
dc.subject.meshInfluenza, Human - immunology-
dc.titleDifferences in antibody responses of individuals with natural infection and those vaccinated against pandemic H1N1 2009 influenzaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1556-6811&volume=18&issue=5&spage=867&epage=873&date=2011&atitle=Differences+in+antibody+responses+of+individuals+with+natural+infection+and+those+vaccinated+against+Pandemic+H1N1+2009+Influenza-
dc.identifier.emailTo, KKW: kelvinto@hkucc.hku.hken_HK
dc.identifier.emailHung, IFN: ivanhung@hkucc.hku.hken_HK
dc.identifier.emailZhang, AJX: zhangajx@hkucc.hku.hken_HK
dc.identifier.emailChan, JFW: jfwchan@hku.hken_HK
dc.identifier.emailTse, H: htse@hkucc.hku.hken_HK
dc.identifier.emailChen, H: hlchen@hku.hken_HK
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityTo, KKW=rp01384en_HK
dc.identifier.authorityHung, IFN=rp00508en_HK
dc.identifier.authorityZhang, AJX=rp00413en_HK
dc.identifier.authorityChan, JFW=rp01736en_HK
dc.identifier.authorityTse, H=rp00519en_HK
dc.identifier.authorityChen, H=rp00383en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1128/CVI.00555-10en_HK
dc.identifier.pmid21411604en_HK
dc.identifier.pmcidPMC3122523-
dc.identifier.scopuseid_2-s2.0-79955707809en_HK
dc.identifier.hkuros197223en_US
dc.identifier.hkuros190824en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79955707809&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume18en_HK
dc.identifier.issue5en_HK
dc.identifier.spage867en_HK
dc.identifier.epage873en_HK
dc.identifier.isiWOS:000290156600024-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChan, KH=7406034307en_HK
dc.identifier.scopusauthoridTo, KKW=14323807300en_HK
dc.identifier.scopusauthoridHung, IFN=7006103457en_HK
dc.identifier.scopusauthoridZhang, AJX=12752135600en_HK
dc.identifier.scopusauthoridChan, JFW=24278817900en_HK
dc.identifier.scopusauthoridCheng, VCC=23670479400en_HK
dc.identifier.scopusauthoridTse, H=7006070596en_HK
dc.identifier.scopusauthoridChe, XY=7005743182en_HK
dc.identifier.scopusauthoridChen, H=26643315400en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK

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