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Article: Generation and evaluation of an H9N1 influenza vaccine derived by reverse genetics that allows utilization of a DIVA strategy for control of H9N2 avian influenza
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TitleGeneration and evaluation of an H9N1 influenza vaccine derived by reverse genetics that allows utilization of a DIVA strategy for control of H9N2 avian influenza
 
AuthorsWu, R5 1
Chen, Q5
Zheng, L5
Chen, J5
Sui, Z5
Guan, Y2
Chen, Z5 4 3
 
Issue Date2009
 
PublisherSpringer Wien. The Journal's web site is located at http://www.springer.com/springerwiennewyork/medicine/journal/705
 
CitationArchives Of Virology, 2009, v. 154 n. 8, p. 1203-1210 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s00705-009-0425-6
 
AbstractH9N2 avian influenza viruses have circulated widely in domestic poultry around the world, and their outbreaks have resulted in heavy morbidity and mortality. In addition, H9N2 avian influenza viruses were transmitted directly from birds to humans in Hong Kong and mainland China during 1998 and 2003, which prompted the public health authorities to seek protective strategies to control H9N2 influenza viruses. In this study, we attempted to develop a DIVA (differentiating infected and vaccinated animals) strategy for H9N2 avian influenza viruses. This strategy does not interfere with serological monitoring and allows effective control of H9N2 avian influenza. We generated a reassortant H9N1 influenza vaccine strain by reverse genetics and employed an enzyme-linked immunosorbent assay (ELISA) with a truncated N1 antigen expressed in E. coli to differentiate between vaccinated and naturally infected animals. Immunization of BALB/c mice with the inactivated reassortant H9N1 vaccine conferred protection against lethal challenge with H9N2 viruses. Meanwhile, the ELISA can be used to distinguish between vaccination and natural infection quickly and easily. Therefore, this study has opened up a new avenue for the control of H9N2 avian influenza. © Springer-Verlag 2009.
 
ISSN0304-8608
2013 Impact Factor: 2.282
 
DOIhttp://dx.doi.org/10.1007/s00705-009-0425-6
 
ISI Accession Number IDWOS:000269687400003
Funding AgencyGrant Number
European Union projectSP5B-CT-2006-044161
National 973 Project2005CB523007
2006CB933102
Chinese Academy of SciencesKSCX1-YW-R-14
Hunan Provincial Science and Technology Department2006NK2003
National Key Technology R& D Program of China2006BAD06A03
Science and Technology Commission of Shanghai Municipality064319030
Funding Information:

We thank R. G. Webster ( from St. Jude Children's Research Hospital, Memphis, TN) for the pHW2000 plasmid. This study was supported by the following research funds: European Union project (SP5B-CT-2006-044161); National 973 Project (2005CB523007, 2006CB933102); Chinese Academy of Sciences (KSCX1-YW-R-14); Hunan Provincial Science and Technology Department (2006NK2003); National Key Technology R& D Program of China (2006BAD06A03); Science and Technology Commission of Shanghai Municipality ( 064319030).

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorWu, R
 
dc.contributor.authorChen, Q
 
dc.contributor.authorZheng, L
 
dc.contributor.authorChen, J
 
dc.contributor.authorSui, Z
 
dc.contributor.authorGuan, Y
 
dc.contributor.authorChen, Z
 
dc.date.accessioned2011-10-28T02:45:32Z
 
dc.date.available2011-10-28T02:45:32Z
 
dc.date.issued2009
 
dc.description.abstractH9N2 avian influenza viruses have circulated widely in domestic poultry around the world, and their outbreaks have resulted in heavy morbidity and mortality. In addition, H9N2 avian influenza viruses were transmitted directly from birds to humans in Hong Kong and mainland China during 1998 and 2003, which prompted the public health authorities to seek protective strategies to control H9N2 influenza viruses. In this study, we attempted to develop a DIVA (differentiating infected and vaccinated animals) strategy for H9N2 avian influenza viruses. This strategy does not interfere with serological monitoring and allows effective control of H9N2 avian influenza. We generated a reassortant H9N1 influenza vaccine strain by reverse genetics and employed an enzyme-linked immunosorbent assay (ELISA) with a truncated N1 antigen expressed in E. coli to differentiate between vaccinated and naturally infected animals. Immunization of BALB/c mice with the inactivated reassortant H9N1 vaccine conferred protection against lethal challenge with H9N2 viruses. Meanwhile, the ELISA can be used to distinguish between vaccination and natural infection quickly and easily. Therefore, this study has opened up a new avenue for the control of H9N2 avian influenza. © Springer-Verlag 2009.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationArchives Of Virology, 2009, v. 154 n. 8, p. 1203-1210 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s00705-009-0425-6
 
dc.identifier.citeulike5053794
 
dc.identifier.doihttp://dx.doi.org/10.1007/s00705-009-0425-6
 
dc.identifier.epage1210
 
dc.identifier.hkuros196833
 
dc.identifier.isiWOS:000269687400003
Funding AgencyGrant Number
European Union projectSP5B-CT-2006-044161
National 973 Project2005CB523007
2006CB933102
Chinese Academy of SciencesKSCX1-YW-R-14
Hunan Provincial Science and Technology Department2006NK2003
National Key Technology R& D Program of China2006BAD06A03
Science and Technology Commission of Shanghai Municipality064319030
Funding Information:

We thank R. G. Webster ( from St. Jude Children's Research Hospital, Memphis, TN) for the pHW2000 plasmid. This study was supported by the following research funds: European Union project (SP5B-CT-2006-044161); National 973 Project (2005CB523007, 2006CB933102); Chinese Academy of Sciences (KSCX1-YW-R-14); Hunan Provincial Science and Technology Department (2006NK2003); National Key Technology R& D Program of China (2006BAD06A03); Science and Technology Commission of Shanghai Municipality ( 064319030).

 
dc.identifier.issn0304-8608
2013 Impact Factor: 2.282
 
dc.identifier.issue8
 
dc.identifier.openurl
 
dc.identifier.pmid19543688
 
dc.identifier.scopuseid_2-s2.0-70349569546
 
dc.identifier.spage1203
 
dc.identifier.urihttp://hdl.handle.net/10722/142414
 
dc.identifier.volume154
 
dc.languageeng
 
dc.publisherSpringer Wien. The Journal's web site is located at http://www.springer.com/springerwiennewyork/medicine/journal/705
 
dc.publisher.placeAustria
 
dc.relation.ispartofArchives of Virology
 
dc.relation.referencesReferences in Scopus
 
dc.rightsThe original publication is available at www.springerlink.com
 
dc.subject.meshInfluenza A Virus, H9N2 Subtype - immunology
 
dc.subject.meshInfluenza Vaccines - administration and dosage - immunology
 
dc.subject.meshInfluenza in Birds - immunology - prevention and control
 
dc.subject.meshInfluenza, Human - immunology - prevention and control
 
dc.subject.meshOrthomyxoviridae Infections - immunology - prevention and control
 
dc.titleGeneration and evaluation of an H9N1 influenza vaccine derived by reverse genetics that allows utilization of a DIVA strategy for control of H9N2 avian influenza
 
dc.typeArticle
 
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Author Affiliations
  1. Graduate University of Chinese Academy of Sciences
  2. The University of Hong Kong
  3. Shanghai Institute of Biological Products
  4. Hunan Normal University
  5. Wuhan Institute of Virology Chinese Academy of Sciences