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Article: Generation and evaluation of an H9N1 influenza vaccine derived by reverse genetics that allows utilization of a DIVA strategy for control of H9N2 avian influenza

TitleGeneration and evaluation of an H9N1 influenza vaccine derived by reverse genetics that allows utilization of a DIVA strategy for control of H9N2 avian influenza
Authors
Issue Date2009
PublisherSpringer Wien. The Journal's web site is located at http://www.springer.com/springerwiennewyork/medicine/journal/705
Citation
Archives Of Virology, 2009, v. 154 n. 8, p. 1203-1210 How to Cite?
AbstractH9N2 avian influenza viruses have circulated widely in domestic poultry around the world, and their outbreaks have resulted in heavy morbidity and mortality. In addition, H9N2 avian influenza viruses were transmitted directly from birds to humans in Hong Kong and mainland China during 1998 and 2003, which prompted the public health authorities to seek protective strategies to control H9N2 influenza viruses. In this study, we attempted to develop a DIVA (differentiating infected and vaccinated animals) strategy for H9N2 avian influenza viruses. This strategy does not interfere with serological monitoring and allows effective control of H9N2 avian influenza. We generated a reassortant H9N1 influenza vaccine strain by reverse genetics and employed an enzyme-linked immunosorbent assay (ELISA) with a truncated N1 antigen expressed in E. coli to differentiate between vaccinated and naturally infected animals. Immunization of BALB/c mice with the inactivated reassortant H9N1 vaccine conferred protection against lethal challenge with H9N2 viruses. Meanwhile, the ELISA can be used to distinguish between vaccination and natural infection quickly and easily. Therefore, this study has opened up a new avenue for the control of H9N2 avian influenza. © Springer-Verlag 2009.
Persistent Identifierhttp://hdl.handle.net/10722/142414
ISSN
2014 Impact Factor: 2.390
2014 SCImago Journal Rankings: 0.945
ISI Accession Number ID
Funding AgencyGrant Number
European Union projectSP5B-CT-2006-044161
National 973 Project2005CB523007
2006CB933102
Chinese Academy of SciencesKSCX1-YW-R-14
Hunan Provincial Science and Technology Department2006NK2003
National Key Technology R& D Program of China2006BAD06A03
Science and Technology Commission of Shanghai Municipality064319030
Funding Information:

We thank R. G. Webster ( from St. Jude Children's Research Hospital, Memphis, TN) for the pHW2000 plasmid. This study was supported by the following research funds: European Union project (SP5B-CT-2006-044161); National 973 Project (2005CB523007, 2006CB933102); Chinese Academy of Sciences (KSCX1-YW-R-14); Hunan Provincial Science and Technology Department (2006NK2003); National Key Technology R& D Program of China (2006BAD06A03); Science and Technology Commission of Shanghai Municipality ( 064319030).

References

 

DC FieldValueLanguage
dc.contributor.authorWu, Ren_HK
dc.contributor.authorChen, Qen_HK
dc.contributor.authorZheng, Len_HK
dc.contributor.authorChen, Jen_HK
dc.contributor.authorSui, Zen_HK
dc.contributor.authorGuan, Yen_HK
dc.contributor.authorChen, Zen_HK
dc.date.accessioned2011-10-28T02:45:32Z-
dc.date.available2011-10-28T02:45:32Z-
dc.date.issued2009en_HK
dc.identifier.citationArchives Of Virology, 2009, v. 154 n. 8, p. 1203-1210en_HK
dc.identifier.issn0304-8608en_HK
dc.identifier.urihttp://hdl.handle.net/10722/142414-
dc.description.abstractH9N2 avian influenza viruses have circulated widely in domestic poultry around the world, and their outbreaks have resulted in heavy morbidity and mortality. In addition, H9N2 avian influenza viruses were transmitted directly from birds to humans in Hong Kong and mainland China during 1998 and 2003, which prompted the public health authorities to seek protective strategies to control H9N2 influenza viruses. In this study, we attempted to develop a DIVA (differentiating infected and vaccinated animals) strategy for H9N2 avian influenza viruses. This strategy does not interfere with serological monitoring and allows effective control of H9N2 avian influenza. We generated a reassortant H9N1 influenza vaccine strain by reverse genetics and employed an enzyme-linked immunosorbent assay (ELISA) with a truncated N1 antigen expressed in E. coli to differentiate between vaccinated and naturally infected animals. Immunization of BALB/c mice with the inactivated reassortant H9N1 vaccine conferred protection against lethal challenge with H9N2 viruses. Meanwhile, the ELISA can be used to distinguish between vaccination and natural infection quickly and easily. Therefore, this study has opened up a new avenue for the control of H9N2 avian influenza. © Springer-Verlag 2009.en_HK
dc.languageengen_US
dc.publisherSpringer Wien. The Journal's web site is located at http://www.springer.com/springerwiennewyork/medicine/journal/705en_HK
dc.relation.ispartofArchives of Virologyen_HK
dc.rightsThe original publication is available at www.springerlink.com-
dc.subject.meshInfluenza A Virus, H9N2 Subtype - immunology-
dc.subject.meshInfluenza Vaccines - administration and dosage - immunology-
dc.subject.meshInfluenza in Birds - immunology - prevention and control-
dc.subject.meshInfluenza, Human - immunology - prevention and control-
dc.subject.meshOrthomyxoviridae Infections - immunology - prevention and control-
dc.titleGeneration and evaluation of an H9N1 influenza vaccine derived by reverse genetics that allows utilization of a DIVA strategy for control of H9N2 avian influenzaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0304-8608&volume=154&issue=8&spage=1203&epage=1210&date=2009&atitle=Generation+and+evaluation+of+an+H9N1+influenza+vaccine+derived+by+reverse+genetics+that+allows+utilization+of+a+DIVA+strategy+for+control+of+H9N2+avian+influenza-
dc.identifier.emailGuan, Y: yguan@hkucc.hku.hken_HK
dc.identifier.authorityGuan, Y=rp00397en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s00705-009-0425-6en_HK
dc.identifier.pmid19543688-
dc.identifier.scopuseid_2-s2.0-70349569546en_HK
dc.identifier.hkuros196833en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-70349569546&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume154en_HK
dc.identifier.issue8en_HK
dc.identifier.spage1203en_HK
dc.identifier.epage1210en_HK
dc.identifier.isiWOS:000269687400003-
dc.publisher.placeAustriaen_HK
dc.identifier.scopusauthoridWu, R=36124196300en_HK
dc.identifier.scopusauthoridChen, Q=13008509400en_HK
dc.identifier.scopusauthoridZheng, L=35365717800en_HK
dc.identifier.scopusauthoridChen, J=35070971200en_HK
dc.identifier.scopusauthoridSui, Z=26647929600en_HK
dc.identifier.scopusauthoridGuan, Y=7202924055en_HK
dc.identifier.scopusauthoridChen, Z=13609597000en_HK
dc.identifier.citeulike5053794-

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