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Article: A live bivalent influenza vaccine based on a H9N2 virus strain

TitleA live bivalent influenza vaccine based on a H9N2 virus strain
Authors
KeywordsBivalent vaccine
H9N2
Hemagglutinin
Influenza
Issue Date2010
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/vaccine
Citation
Vaccine, 2010, v. 28 n. 3, p. 673-680 How to Cite?
AbstractThe purpose of this study was to construct an H9N2 virus-based bivalent live vaccine expressing the protective antigen of a different subtype of influenza virus. Reverse genetics was used to generate an influenza virus containing nine gene segments derived from the A/Chicken/Jiangsu/11/2002 (H9N2) strain, including independent M1 and M2 matrix gene segments. A recombinant virus expressing the H1N1 HA1 hemagglutinin protein was produced on this framework by substituting the extracellular domain of the H9N2 M2 gene with the H1N1 HA1 fragment from A/PR/8/34 (PR8, H1N1). The resulting hybrid virus H9N2-PR8/HA1 was genetically stable and of low pathogenicity. Intra-nasal immunization of BALB/c mice with H9N2-PR8/HA1 virus induced both anti-H9N2 virus and anti-PR8 HA antibodies and conferred protection to mice against lethal challenge (40× LD 50) with either H1N1 or H9N2 viruses. This study provides a new influenza H9N2 virus model for the expression and/or delivery of foreign antigens. © 2009 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/142413
ISSN
2015 Impact Factor: 3.413
2015 SCImago Journal Rankings: 2.044
ISI Accession Number ID
Funding AgencyGrant Number
European UnionSP5B-Cr-2006-044161
National 9732005CB523007
2006CB933102
Chinese Academy of SciencesKSCX1-YW-R-14
Hunan Provincial Science and Technology Department2006NK2003
National Key Technology R&D Program of China2006BAD06A03
Science and Technology Commission of Shanghai Municipality064319030
Funding Information:

We thank R.G. Webster (from St. Jude Children's Research Hospital, Memphis, TN) for the pHW2000 plasmid. This study was supported by the following research funds: European Union project (SP5B-Cr-2006-044161); National 973 Project (2005CB523007, 2006CB933102): s (KSCX1-YW-R-14); Hunan Provincial Science and Technology Department (Chinese Academy of Science2006NK2003); National Key Technology R&D Program of China (2006BAD06A03); Science and Technology Commission of Shanghai Municipality (064319030).

References

 

DC FieldValueLanguage
dc.contributor.authorWu, Ren_HK
dc.contributor.authorGuan, Yen_HK
dc.contributor.authorYang, Zen_HK
dc.contributor.authorChen, Jen_HK
dc.contributor.authorWang, Hen_HK
dc.contributor.authorChen, Qen_HK
dc.contributor.authorSui, Zen_HK
dc.contributor.authorFang, Fen_HK
dc.contributor.authorChen, Zen_HK
dc.date.accessioned2011-10-28T02:45:29Z-
dc.date.available2011-10-28T02:45:29Z-
dc.date.issued2010en_HK
dc.identifier.citationVaccine, 2010, v. 28 n. 3, p. 673-680en_HK
dc.identifier.issn0264-410Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/142413-
dc.description.abstractThe purpose of this study was to construct an H9N2 virus-based bivalent live vaccine expressing the protective antigen of a different subtype of influenza virus. Reverse genetics was used to generate an influenza virus containing nine gene segments derived from the A/Chicken/Jiangsu/11/2002 (H9N2) strain, including independent M1 and M2 matrix gene segments. A recombinant virus expressing the H1N1 HA1 hemagglutinin protein was produced on this framework by substituting the extracellular domain of the H9N2 M2 gene with the H1N1 HA1 fragment from A/PR/8/34 (PR8, H1N1). The resulting hybrid virus H9N2-PR8/HA1 was genetically stable and of low pathogenicity. Intra-nasal immunization of BALB/c mice with H9N2-PR8/HA1 virus induced both anti-H9N2 virus and anti-PR8 HA antibodies and conferred protection to mice against lethal challenge (40× LD 50) with either H1N1 or H9N2 viruses. This study provides a new influenza H9N2 virus model for the expression and/or delivery of foreign antigens. © 2009 Elsevier Ltd. All rights reserved.en_HK
dc.languageengen_US
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/vaccineen_HK
dc.relation.ispartofVaccineen_HK
dc.subjectBivalent vaccineen_HK
dc.subjectH9N2en_HK
dc.subjectHemagglutininen_HK
dc.subjectInfluenzaen_HK
dc.subject.meshAntibodies, Viral - blood-
dc.subject.meshGenome, Viral-
dc.subject.meshInfluenza A Virus, H1N1 Subtype - genetics - immunology - pathogenicity-
dc.subject.meshInfluenza A Virus, H9N2 Subtype - genetics - immunology - pathogenicity-
dc.subject.meshInfluenza Vaccines - administration and dosage - genetics - immunology-
dc.titleA live bivalent influenza vaccine based on a H9N2 virus strainen_HK
dc.typeArticleen_HK
dc.identifier.emailGuan, Y: yguan@hkucc.hku.hken_HK
dc.identifier.authorityGuan, Y=rp00397en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.vaccine.2009.10.102en_HK
dc.identifier.pmid19892041-
dc.identifier.scopuseid_2-s2.0-71149104684en_HK
dc.identifier.hkuros196831en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-71149104684&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume28en_HK
dc.identifier.issue3en_HK
dc.identifier.spage673en_HK
dc.identifier.epage680en_HK
dc.identifier.isiWOS:000275015200011-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridWu, R=36124196300en_HK
dc.identifier.scopusauthoridGuan, Y=7202924055en_HK
dc.identifier.scopusauthoridYang, Z=25026376700en_HK
dc.identifier.scopusauthoridChen, J=35070971200en_HK
dc.identifier.scopusauthoridWang, H=22942629300en_HK
dc.identifier.scopusauthoridChen, Q=13008509400en_HK
dc.identifier.scopusauthoridSui, Z=26647929600en_HK
dc.identifier.scopusauthoridFang, F=7202929817en_HK
dc.identifier.scopusauthoridChen, Z=13609597000en_HK

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