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Article: Early gene expression events in ferrets in response to SARS coronavirus infection versus direct interferon-alpha2b stimulation
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TitleEarly gene expression events in ferrets in response to SARS coronavirus infection versus direct interferon-alpha2b stimulation
 
AuthorsDanesh, A1 2
Cameron, CM1
León, AJ4
Ran, L1
Xu, L1
Fang, Y1 2
Kelvin, AA6 7
Rowe, T1 5
Chen, H4
Guan, Y4
Jonsson, CB8
Cameron, MJ1
Kelvin, DJ4 3 1
 
KeywordsFerret
Gene expression
Interferon
SARS
 
Issue Date2011
 
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yviro
 
CitationVirology, 2011, v. 409 n. 1, p. 102-112 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.virol.2010.10.002
 
AbstractType I interferons (IFNs) are essential to the clearance of viral diseases, however, a clear distinction between genes upregulated by direct virus-cell interactions and genes upregulated by secondary IFN production has not been made. Here, we investigated differential gene regulation in ferrets upon subcutaneous administration of IFN-α2b and during SARS-CoV infection. In vivo experiments revealed that IFN-α2b causes STAT1 phosphorylation and upregulation of abundant IFN response genes (IRGs), chemokine receptors, and other genes that participate in phagocytosis and leukocyte transendothelial migration. During infection with SARS-CoV not only a variety of IRGs were upregulated, but also a significantly broader range of genes involved in cell migration and inflammation. This work allowed dissection of several molecular signatures present during SARS-CoV which are part of a robust IFN antiviral response. These signatures can be useful markers to evaluate the status of IFN responses during a viral infection and specific features of different viruses. © 2010 Elsevier Inc.
 
ISSN0042-6822
2013 Impact Factor: 3.278
 
DOIhttp://dx.doi.org/10.1016/j.virol.2010.10.002
 
ISI Accession Number IDWOS:000285450900013
Funding AgencyGrant Number
Li Ka Shing Foundation, China
NIH/NIAIDNOI-A1-30063C11
Southern Research InstituteNOI-AI-30067
02
Canadian Institute of Health ResearchCIHR-200904PAP-203553-PAM-ADHD-48072
Funding Information:

We are indebted to Nikki Kelvin for her editing and critical review of this manuscript. We also would like to thank Lixia Guo and Zujiang Li for their assistance in cloning of the ferret genes. This project was supported by funding from Li Ka Shing Foundation, China; NIH/NIAID Contract No. NOI-A1-30063C11; Southern Research Institute, Contract No. NOI-AI-30067 Task Order No.02; and the Canadian Institute of Health Research No. CIHR-200904PAP-203553-PAM-ADHD-48072.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorDanesh, A
 
dc.contributor.authorCameron, CM
 
dc.contributor.authorLeón, AJ
 
dc.contributor.authorRan, L
 
dc.contributor.authorXu, L
 
dc.contributor.authorFang, Y
 
dc.contributor.authorKelvin, AA
 
dc.contributor.authorRowe, T
 
dc.contributor.authorChen, H
 
dc.contributor.authorGuan, Y
 
dc.contributor.authorJonsson, CB
 
dc.contributor.authorCameron, MJ
 
dc.contributor.authorKelvin, DJ
 
dc.date.accessioned2011-10-28T02:45:27Z
 
dc.date.available2011-10-28T02:45:27Z
 
dc.date.issued2011
 
dc.description.abstractType I interferons (IFNs) are essential to the clearance of viral diseases, however, a clear distinction between genes upregulated by direct virus-cell interactions and genes upregulated by secondary IFN production has not been made. Here, we investigated differential gene regulation in ferrets upon subcutaneous administration of IFN-α2b and during SARS-CoV infection. In vivo experiments revealed that IFN-α2b causes STAT1 phosphorylation and upregulation of abundant IFN response genes (IRGs), chemokine receptors, and other genes that participate in phagocytosis and leukocyte transendothelial migration. During infection with SARS-CoV not only a variety of IRGs were upregulated, but also a significantly broader range of genes involved in cell migration and inflammation. This work allowed dissection of several molecular signatures present during SARS-CoV which are part of a robust IFN antiviral response. These signatures can be useful markers to evaluate the status of IFN responses during a viral infection and specific features of different viruses. © 2010 Elsevier Inc.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationVirology, 2011, v. 409 n. 1, p. 102-112 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.virol.2010.10.002
 
dc.identifier.citeulike8189203
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.virol.2010.10.002
 
dc.identifier.epage112
 
dc.identifier.hkuros196759
 
dc.identifier.isiWOS:000285450900013
Funding AgencyGrant Number
Li Ka Shing Foundation, China
NIH/NIAIDNOI-A1-30063C11
Southern Research InstituteNOI-AI-30067
02
Canadian Institute of Health ResearchCIHR-200904PAP-203553-PAM-ADHD-48072
Funding Information:

We are indebted to Nikki Kelvin for her editing and critical review of this manuscript. We also would like to thank Lixia Guo and Zujiang Li for their assistance in cloning of the ferret genes. This project was supported by funding from Li Ka Shing Foundation, China; NIH/NIAID Contract No. NOI-A1-30063C11; Southern Research Institute, Contract No. NOI-AI-30067 Task Order No.02; and the Canadian Institute of Health Research No. CIHR-200904PAP-203553-PAM-ADHD-48072.

 
dc.identifier.issn0042-6822
2013 Impact Factor: 3.278
 
dc.identifier.issue1
 
dc.identifier.pmid21035159
 
dc.identifier.scopuseid_2-s2.0-78649445187
 
dc.identifier.spage102
 
dc.identifier.urihttp://hdl.handle.net/10722/142410
 
dc.identifier.volume409
 
dc.languageeng
 
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yviro
 
dc.publisher.placeUnited States
 
dc.relation.ispartofVirology
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshDisease Models, Animal
 
dc.subject.meshFerrets - virology
 
dc.subject.meshGene Expression Regulation
 
dc.subject.meshInterferon-alpha - administration and dosage - immunology
 
dc.subject.meshProteins - genetics - metabolism
 
dc.subjectFerret
 
dc.subjectGene expression
 
dc.subjectInterferon
 
dc.subjectSARS
 
dc.titleEarly gene expression events in ferrets in response to SARS coronavirus infection versus direct interferon-alpha2b stimulation
 
dc.typeArticle
 
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<contributor.author>Kelvin, AA</contributor.author>
<contributor.author>Rowe, T</contributor.author>
<contributor.author>Chen, H</contributor.author>
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Author Affiliations
  1. Toronto General Research Institute University of Toronto
  2. University of Toronto Faculty of Medicine
  3. Università degli Studi di Sassari
  4. Shantou University, Medical College (SUMC)
  5. Centers for Disease Control and Prevention
  6. Sardinia Research and Development srl
  7. IDR
  8. Center for Predictive Medicine