File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Early gene expression events in ferrets in response to SARS coronavirus infection versus direct interferon-alpha2b stimulation

TitleEarly gene expression events in ferrets in response to SARS coronavirus infection versus direct interferon-alpha2b stimulation
Authors
KeywordsFerret
Gene expression
Interferon
SARS
Issue Date2011
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yviro
Citation
Virology, 2011, v. 409 n. 1, p. 102-112 How to Cite?
Abstract
Type I interferons (IFNs) are essential to the clearance of viral diseases, however, a clear distinction between genes upregulated by direct virus-cell interactions and genes upregulated by secondary IFN production has not been made. Here, we investigated differential gene regulation in ferrets upon subcutaneous administration of IFN-α2b and during SARS-CoV infection. In vivo experiments revealed that IFN-α2b causes STAT1 phosphorylation and upregulation of abundant IFN response genes (IRGs), chemokine receptors, and other genes that participate in phagocytosis and leukocyte transendothelial migration. During infection with SARS-CoV not only a variety of IRGs were upregulated, but also a significantly broader range of genes involved in cell migration and inflammation. This work allowed dissection of several molecular signatures present during SARS-CoV which are part of a robust IFN antiviral response. These signatures can be useful markers to evaluate the status of IFN responses during a viral infection and specific features of different viruses. © 2010 Elsevier Inc.
Persistent Identifierhttp://hdl.handle.net/10722/142410
ISSN
2013 Impact Factor: 3.278
ISI Accession Number ID
Funding AgencyGrant Number
Li Ka Shing Foundation, China
NIH/NIAIDNOI-A1-30063C11
Southern Research InstituteNOI-AI-30067
02
Canadian Institute of Health ResearchCIHR-200904PAP-203553-PAM-ADHD-48072
Funding Information:

We are indebted to Nikki Kelvin for her editing and critical review of this manuscript. We also would like to thank Lixia Guo and Zujiang Li for their assistance in cloning of the ferret genes. This project was supported by funding from Li Ka Shing Foundation, China; NIH/NIAID Contract No. NOI-A1-30063C11; Southern Research Institute, Contract No. NOI-AI-30067 Task Order No.02; and the Canadian Institute of Health Research No. CIHR-200904PAP-203553-PAM-ADHD-48072.

References

 

Author Affiliations
  1. Toronto General Research Institute University of Toronto
  2. University of Toronto Faculty of Medicine
  3. Università degli Studi di Sassari
  4. Shantou University, Medical College (SUMC)
  5. Centers for Disease Control and Prevention
  6. Sardinia Research and Development srl
  7. IDR
  8. Center for Predictive Medicine
DC FieldValueLanguage
dc.contributor.authorDanesh, Aen_HK
dc.contributor.authorCameron, CMen_HK
dc.contributor.authorLeón, AJen_HK
dc.contributor.authorRan, Len_HK
dc.contributor.authorXu, Len_HK
dc.contributor.authorFang, Yen_HK
dc.contributor.authorKelvin, AAen_HK
dc.contributor.authorRowe, Ten_HK
dc.contributor.authorChen, Hen_HK
dc.contributor.authorGuan, Yen_HK
dc.contributor.authorJonsson, CBen_HK
dc.contributor.authorCameron, MJen_HK
dc.contributor.authorKelvin, DJen_HK
dc.date.accessioned2011-10-28T02:45:27Z-
dc.date.available2011-10-28T02:45:27Z-
dc.date.issued2011en_HK
dc.identifier.citationVirology, 2011, v. 409 n. 1, p. 102-112en_HK
dc.identifier.issn0042-6822en_HK
dc.identifier.urihttp://hdl.handle.net/10722/142410-
dc.description.abstractType I interferons (IFNs) are essential to the clearance of viral diseases, however, a clear distinction between genes upregulated by direct virus-cell interactions and genes upregulated by secondary IFN production has not been made. Here, we investigated differential gene regulation in ferrets upon subcutaneous administration of IFN-α2b and during SARS-CoV infection. In vivo experiments revealed that IFN-α2b causes STAT1 phosphorylation and upregulation of abundant IFN response genes (IRGs), chemokine receptors, and other genes that participate in phagocytosis and leukocyte transendothelial migration. During infection with SARS-CoV not only a variety of IRGs were upregulated, but also a significantly broader range of genes involved in cell migration and inflammation. This work allowed dissection of several molecular signatures present during SARS-CoV which are part of a robust IFN antiviral response. These signatures can be useful markers to evaluate the status of IFN responses during a viral infection and specific features of different viruses. © 2010 Elsevier Inc.en_HK
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yviroen_HK
dc.relation.ispartofVirologyen_HK
dc.subjectFerreten_HK
dc.subjectGene expressionen_HK
dc.subjectInterferonen_HK
dc.subjectSARSen_HK
dc.subject.meshDisease Models, Animal-
dc.subject.meshFerrets - virology-
dc.subject.meshGene Expression Regulation-
dc.subject.meshInterferon-alpha - administration and dosage - immunology-
dc.subject.meshProteins - genetics - metabolism-
dc.titleEarly gene expression events in ferrets in response to SARS coronavirus infection versus direct interferon-alpha2b stimulationen_HK
dc.typeArticleen_HK
dc.identifier.emailChen, H: hlchen@hku.hken_HK
dc.identifier.emailGuan, Y: yguan@hkucc.hku.hken_HK
dc.identifier.authorityChen, H=rp00383en_HK
dc.identifier.authorityGuan, Y=rp00397en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.virol.2010.10.002en_HK
dc.identifier.pmid21035159en_HK
dc.identifier.scopuseid_2-s2.0-78649445187en_HK
dc.identifier.hkuros196759en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-78649445187&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume409en_HK
dc.identifier.issue1en_HK
dc.identifier.spage102en_HK
dc.identifier.epage112en_HK
dc.identifier.isiWOS:000285450900013-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridDanesh, A=10639081200en_HK
dc.identifier.scopusauthoridCameron, CM=8770762400en_HK
dc.identifier.scopusauthoridLeón, AJ=14062184100en_HK
dc.identifier.scopusauthoridRan, L=7006581232en_HK
dc.identifier.scopusauthoridXu, L=7404744742en_HK
dc.identifier.scopusauthoridFang, Y=35745989100en_HK
dc.identifier.scopusauthoridKelvin, AA=6603814164en_HK
dc.identifier.scopusauthoridRowe, T=7102561610en_HK
dc.identifier.scopusauthoridChen, H=26643315400en_HK
dc.identifier.scopusauthoridGuan, Y=7202924055en_HK
dc.identifier.scopusauthoridJonsson, CB=7102791844en_HK
dc.identifier.scopusauthoridCameron, MJ=7102724879en_HK
dc.identifier.scopusauthoridKelvin, DJ=7006326577en_HK
dc.identifier.citeulike8189203-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats