Article: Early gene expression events in ferrets in response to SARS coronavirus infection versus direct interferon-alpha2b stimulation

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TitleEarly gene expression events in ferrets in response to SARS coronavirus infection versus direct interferon-alpha2b stimulation
AuthorsDanesh, A1 2
Cameron, CM1
León, AJ4
Ran, L1
Xu, L1
Fang, Y1 2
Kelvin, AA6 7
Rowe, T1 5
Chen, H4
Guan, Y4
Jonsson, CB8
Cameron, MJ1
Kelvin, DJ1 3 4
KeywordsFerret
Gene expression
Interferon
SARS
Issue Date2011
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yviro
CitationVirology, 2011, v. 409 n. 1, p. 102-112 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.virol.2010.10.002
AbstractType I interferons (IFNs) are essential to the clearance of viral diseases, however, a clear distinction between genes upregulated by direct virus-cell interactions and genes upregulated by secondary IFN production has not been made. Here, we investigated differential gene regulation in ferrets upon subcutaneous administration of IFN-α2b and during SARS-CoV infection. In vivo experiments revealed that IFN-α2b causes STAT1 phosphorylation and upregulation of abundant IFN response genes (IRGs), chemokine receptors, and other genes that participate in phagocytosis and leukocyte transendothelial migration. During infection with SARS-CoV not only a variety of IRGs were upregulated, but also a significantly broader range of genes involved in cell migration and inflammation. This work allowed dissection of several molecular signatures present during SARS-CoV which are part of a robust IFN antiviral response. These signatures can be useful markers to evaluate the status of IFN responses during a viral infection and specific features of different viruses. © 2010 Elsevier Inc.
ISSN0042-6822
2011 Impact Factor: 3.351
2011 SCImago Journal Rankings: 0.355
DOIhttp://dx.doi.org/10.1016/j.virol.2010.10.002
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorDanesh, A
dc.contributor.authorCameron, CM
dc.contributor.authorLeón, AJ
dc.contributor.authorRan, L
dc.contributor.authorXu, L
dc.contributor.authorFang, Y
dc.contributor.authorKelvin, AA
dc.contributor.authorRowe, T
dc.contributor.authorChen, H
dc.contributor.authorGuan, Y
dc.contributor.authorJonsson, CB
dc.contributor.authorCameron, MJ
dc.contributor.authorKelvin, DJ
dc.date.accessioned2011-10-28T02:45:27Z
dc.date.available2011-10-28T02:45:27Z
dc.date.issued2011
dc.description.abstractType I interferons (IFNs) are essential to the clearance of viral diseases, however, a clear distinction between genes upregulated by direct virus-cell interactions and genes upregulated by secondary IFN production has not been made. Here, we investigated differential gene regulation in ferrets upon subcutaneous administration of IFN-α2b and during SARS-CoV infection. In vivo experiments revealed that IFN-α2b causes STAT1 phosphorylation and upregulation of abundant IFN response genes (IRGs), chemokine receptors, and other genes that participate in phagocytosis and leukocyte transendothelial migration. During infection with SARS-CoV not only a variety of IRGs were upregulated, but also a significantly broader range of genes involved in cell migration and inflammation. This work allowed dissection of several molecular signatures present during SARS-CoV which are part of a robust IFN antiviral response. These signatures can be useful markers to evaluate the status of IFN responses during a viral infection and specific features of different viruses. © 2010 Elsevier Inc.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationVirology, 2011, v. 409 n. 1, p. 102-112 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.virol.2010.10.002
dc.identifier.citeulike8189203
dc.identifier.doihttp://dx.doi.org/10.1016/j.virol.2010.10.002
dc.identifier.epage112
dc.identifier.hkuros196759
dc.identifier.isiWOS:000285450900013
Funding AgencyGrant Number
Li Ka Shing Foundation, China
NIH/NIAIDNOI-A1-30063C11
Southern Research InstituteNOI-AI-30067
02
Canadian Institute of Health ResearchCIHR-200904PAP-203553-PAM-ADHD-48072
Funding Information:

We are indebted to Nikki Kelvin for her editing and critical review of this manuscript. We also would like to thank Lixia Guo and Zujiang Li for their assistance in cloning of the ferret genes. This project was supported by funding from Li Ka Shing Foundation, China; NIH/NIAID Contract No. NOI-A1-30063C11; Southern Research Institute, Contract No. NOI-AI-30067 Task Order No.02; and the Canadian Institute of Health Research No. CIHR-200904PAP-203553-PAM-ADHD-48072.

dc.identifier.issn0042-6822
2011 Impact Factor: 3.351
2011 SCImago Journal Rankings: 0.355
dc.identifier.issue1
dc.identifier.pmid21035159
dc.identifier.scopuseid_2-s2.0-78649445187
dc.identifier.spage102
dc.identifier.urihttp://hdl.handle.net/10722/142410
dc.identifier.volume409
dc.languageeng
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yviro
dc.publisher.placeUnited States
dc.relation.ispartofVirology
dc.relation.referencesReferences in Scopus
dc.subject.meshDisease Models, Animal
dc.subject.meshFerrets - virology
dc.subject.meshGene Expression Regulation
dc.subject.meshInterferon-alpha - administration and dosage - immunology
dc.subject.meshProteins - genetics - metabolism
dc.subjectFerret
dc.subjectGene expression
dc.subjectInterferon
dc.subjectSARS
dc.titleEarly gene expression events in ferrets in response to SARS coronavirus infection versus direct interferon-alpha2b stimulation
dc.typeArticle
Author Affiliations
  1. Toronto General Research Institute
  2. University of Toronto, Faculty of Medicine
  3. Università degli Studi di Sassari
  4. Shantou University, Medical College (SUMC)
  5. Centers for Disease Control and Prevention
  6. Sardinia Research and Development srl
  7. IDR
  8. Center for Predictive Medicine