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Article: Adrenomedullin in rat follicles and corpora lutea: Expression, functions and interaction with endothelin-1

TitleAdrenomedullin in rat follicles and corpora lutea: Expression, functions and interaction with endothelin-1
Authors
Issue Date2011
PublisherBioMed Central Ltd. The Journal's web site is located at http://www.rbej.com/home
Citation
Reproductive Biology And Endocrinology, 2011, v. 9 How to Cite?
AbstractBackground: Adrenomedullin (ADM), a novel vasorelaxant peptide, was found in human/rat ovaries. The present study investigated the interaction of ADM and endothelin-1 (ET-1) in follicles and newly formed corpora lutea (CL) and the actions of ADM on progesterone production in CL during pregnancy.Methods: The peptide and gene expression level of adrenomedullin in small antral follicles, large antral follicles and CL was studied by real-time RT-PCR and EIA. The effect of ADM treatment on oestradiol production in 5-day follicular culture and on progesterone production from CL of different pregnant stages was measured by EIA. The interaction of ADM and ET-1 in follicles and CL at their gene expression level was studied by real-time RT-PCR.Results: In the rat ovary, the gene expression of Adm increased during development from small antral follicles to large antral follicles and CL. In vitro treatment of preantral follicular culture for 5 days with ADM increased oestradiol production but did not affect follicular growth or ovulation rate. The regulation of progesterone production by ADM in CL in culture was pregnancy-stage dependent, inhibitory at early and late pregnancy but stimulatory at mid-pregnancy, which might contribute to the high progesterone production rate of the CL at mid-pregnancy. Moreover, the interaction between ADM and ET-1 at both the production and functional levels indicates that these two vasoactive peptides may form an important local, fine-tuning regulatory system together with LH and prolactin for progesterone production in rat CL.Conclusions: As the CL is the major source of progesterone production even after the formation of placenta in rats, ADM may be an important regulator in progesterone production to meet the requirement of pregnancy. © 2011 Li et al; licensee BioMed Central Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/142319
ISSN
2015 Impact Factor: 2.147
2015 SCImago Journal Rankings: 0.919
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, Len_HK
dc.contributor.authorO, WSen_HK
dc.contributor.authorTang, Fen_HK
dc.date.accessioned2011-10-28T02:42:48Z-
dc.date.available2011-10-28T02:42:48Z-
dc.date.issued2011en_HK
dc.identifier.citationReproductive Biology And Endocrinology, 2011, v. 9en_HK
dc.identifier.issn1477-7827en_HK
dc.identifier.urihttp://hdl.handle.net/10722/142319-
dc.description.abstractBackground: Adrenomedullin (ADM), a novel vasorelaxant peptide, was found in human/rat ovaries. The present study investigated the interaction of ADM and endothelin-1 (ET-1) in follicles and newly formed corpora lutea (CL) and the actions of ADM on progesterone production in CL during pregnancy.Methods: The peptide and gene expression level of adrenomedullin in small antral follicles, large antral follicles and CL was studied by real-time RT-PCR and EIA. The effect of ADM treatment on oestradiol production in 5-day follicular culture and on progesterone production from CL of different pregnant stages was measured by EIA. The interaction of ADM and ET-1 in follicles and CL at their gene expression level was studied by real-time RT-PCR.Results: In the rat ovary, the gene expression of Adm increased during development from small antral follicles to large antral follicles and CL. In vitro treatment of preantral follicular culture for 5 days with ADM increased oestradiol production but did not affect follicular growth or ovulation rate. The regulation of progesterone production by ADM in CL in culture was pregnancy-stage dependent, inhibitory at early and late pregnancy but stimulatory at mid-pregnancy, which might contribute to the high progesterone production rate of the CL at mid-pregnancy. Moreover, the interaction between ADM and ET-1 at both the production and functional levels indicates that these two vasoactive peptides may form an important local, fine-tuning regulatory system together with LH and prolactin for progesterone production in rat CL.Conclusions: As the CL is the major source of progesterone production even after the formation of placenta in rats, ADM may be an important regulator in progesterone production to meet the requirement of pregnancy. © 2011 Li et al; licensee BioMed Central Ltd.en_HK
dc.languageengen_US
dc.publisherBioMed Central Ltd. The Journal's web site is located at http://www.rbej.com/homeen_HK
dc.relation.ispartofReproductive Biology and Endocrinologyen_HK
dc.rightsReproductive Biology and Endocrinology. Copyright © BioMed Central Ltd.-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshAdrenomedullin - genetics - metabolism - pharmacology - secretion-
dc.subject.meshCorpus Luteum - drug effects - growth and development - metabolism-
dc.subject.meshEndothelin-1 - genetics - metabolism - secretion-
dc.subject.meshOogenesis - drug effects-
dc.subject.meshOvarian Follicle - drug effects - growth and development - metabolism-
dc.titleAdrenomedullin in rat follicles and corpora lutea: Expression, functions and interaction with endothelin-1en_HK
dc.typeArticleen_HK
dc.identifier.emailO, WS: owaisum@hkucc.hku.hken_HK
dc.identifier.emailTang, F: ftang@hkucc.hku.hken_HK
dc.identifier.authorityO, WS=rp00315en_HK
dc.identifier.authorityTang, F=rp00327en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1186/1477-7827-9-111en_HK
dc.identifier.pmid21824440-
dc.identifier.pmcidPMC3175455-
dc.identifier.scopuseid_2-s2.0-80052869492en_HK
dc.identifier.hkuros196907en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80052869492&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume9en_HK
dc.identifier.isiWOS:000295119500001-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLi, L=36071951300en_HK
dc.identifier.scopusauthoridO, WS=6701729369en_HK
dc.identifier.scopusauthoridTang, F=7201979770en_HK
dc.identifier.citeulike9685244-

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