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Article: Multiple pathways were involved in tubeimoside-1-induced cytotoxicity of HeLa cells

TitleMultiple pathways were involved in tubeimoside-1-induced cytotoxicity of HeLa cells
Authors
KeywordsCytoskeleton
Cytotoxicity
ER
Mitochondria
Tubeimoside-1
Issue Date2011
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/713351/description#description
Citation
Journal Of Proteomics, 2011, v. 75 n. 2, p. 491-501 How to Cite?
AbstractThe Bolbostemma paniculatum (Maxim.) Franquet (Cucurbitaceae) is a Chinese herb with anticancer potential. Its main active component tubeimoside-1 (TBMS1), a triterpenoid saponin, was previously proved as a potent anticancer chemotherapeutic agent; however, the molecular basis for its activities is still elusive. In the present study, subcellular proteomic study in the cytoplasm and membrane protein fractions extracted from HeLa cells revealed that proteins act as mediators of ROS generation and Ca 2+ regulation were substantially altered in expression upon TBMS1 stimuli. We also found that TBMS1 induced cell cycle arrest at G2/M phase accompanied by a decrease in G0/G1 phase in HeLa cells. Further biochemical studies showed that TBMS1 inhibited the levels of cyclinB1, Cdc2 and Cdc25C, but enhanced Chk2 phosphorylation. In addition, the cytoplasm sequestration of Cdc25C, Cip1/p21 induction and tubulin dyspolymerization also contributed to the TBMS1-mediated cell cycle arrest on the G2/M phase. © 2011 Elsevier B.V.
Persistent Identifierhttp://hdl.handle.net/10722/142314
ISSN
2015 Impact Factor: 3.867
2015 SCImago Journal Rankings: 1.475
ISI Accession Number ID
Funding AgencyGrant Number
National Natural Science Foundation of China30973393
Fundamental Research Funds for the Central Universities2011121055
HKU Genomics, Proteomics and Bioinformatics Strategic Research Theme, Faculty of Science Development Fund
University of Hong Kong
Natural Science Foundation of Fujian Province of China2011 J05098
Funding Information:

This work was partially supported by the 2007 Chang-Jiang Scholars Program, "211" Projects grant, National Natural Science Foundation of China (30973393), The Fundamental Research Funds for the Central Universities (to Q.-Y.H.); HKU Genomics, Proteomics and Bioinformatics Strategic Research Theme, Faculty of Science Development Fund, the Outstanding Young Researcher and Outstanding Research Student Supervisor Awards of the University of Hong Kong (to F.C.); and the Natural Science Foundation of Fujian Province of China (No. 2011 J05098) and the Fundamental Research Funds for the Central Universities (No. 2011121055) (to Y. X).

References

 

DC FieldValueLanguage
dc.contributor.authorXu, Yen_HK
dc.contributor.authorChing, YPen_HK
dc.contributor.authorZhou, Yen_HK
dc.contributor.authorChiu, JFen_HK
dc.contributor.authorChen, Fen_HK
dc.contributor.authorHe, QYen_HK
dc.date.accessioned2011-10-28T02:42:44Z-
dc.date.available2011-10-28T02:42:44Z-
dc.date.issued2011en_HK
dc.identifier.citationJournal Of Proteomics, 2011, v. 75 n. 2, p. 491-501en_HK
dc.identifier.issn1874-3919en_HK
dc.identifier.urihttp://hdl.handle.net/10722/142314-
dc.description.abstractThe Bolbostemma paniculatum (Maxim.) Franquet (Cucurbitaceae) is a Chinese herb with anticancer potential. Its main active component tubeimoside-1 (TBMS1), a triterpenoid saponin, was previously proved as a potent anticancer chemotherapeutic agent; however, the molecular basis for its activities is still elusive. In the present study, subcellular proteomic study in the cytoplasm and membrane protein fractions extracted from HeLa cells revealed that proteins act as mediators of ROS generation and Ca 2+ regulation were substantially altered in expression upon TBMS1 stimuli. We also found that TBMS1 induced cell cycle arrest at G2/M phase accompanied by a decrease in G0/G1 phase in HeLa cells. Further biochemical studies showed that TBMS1 inhibited the levels of cyclinB1, Cdc2 and Cdc25C, but enhanced Chk2 phosphorylation. In addition, the cytoplasm sequestration of Cdc25C, Cip1/p21 induction and tubulin dyspolymerization also contributed to the TBMS1-mediated cell cycle arrest on the G2/M phase. © 2011 Elsevier B.V.en_HK
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/713351/description#descriptionen_HK
dc.relation.ispartofJournal of Proteomicsen_HK
dc.subjectCytoskeletonen_HK
dc.subjectCytotoxicityen_HK
dc.subjectERen_HK
dc.subjectMitochondriaen_HK
dc.subjectTubeimoside-1en_HK
dc.titleMultiple pathways were involved in tubeimoside-1-induced cytotoxicity of HeLa cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1874-3919&volume=&spage=&epage=&date=2011&atitle=Multiple+pathways+were+involved+in+tubeimoside-1-induced+cytotoxicity+of+HeLa+cellsen_US
dc.identifier.emailChing, YP: ypching@hku.hken_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityChing, YP=rp00469en_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jprot.2011.08.014en_HK
dc.identifier.pmid21903181-
dc.identifier.scopuseid_2-s2.0-82355185714en_HK
dc.identifier.hkuros196627en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-82355185714&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume75en_HK
dc.identifier.issue2en_HK
dc.identifier.spage491en_HK
dc.identifier.epage501en_HK
dc.identifier.isiWOS:000298765500015-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridXu, Y=37035700000en_HK
dc.identifier.scopusauthoridChing, YP=7005431277en_HK
dc.identifier.scopusauthoridZhou, Y=54786121900en_HK
dc.identifier.scopusauthoridChiu, JF=7201501692en_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK
dc.identifier.scopusauthoridHe, QY=34770287900en_HK

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