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Article: Coexpression of adrenomedullin and its receptor component proteins in the reproductive system of the rat during gestation

TitleCoexpression of adrenomedullin and its receptor component proteins in the reproductive system of the rat during gestation
Authors
Issue Date2010
PublisherBioMed Central Ltd. The Journal's web site is located at http://www.rbej.com/home
Citation
Reproductive Biology And Endocrinology, 2010, v. 8 How to Cite?
AbstractBackground: Adrenomedullin (ADM), a novel vasorelaxant peptide, was found in human/rat ovaries and uteri. Plasma ADM level increases in pregnant women and pregnant rats.Methods: The gene expression levels of Adm and its receptor components - Crlr, Ramp1, Ramp2 and Ramp3, the ADM peptide concentration and localization in the rat female reproductive system during gestation were studied by real-time RT-PCR, EIA and immunohistochemical techniques.Results: The mRNAs of Adm and its receptor component and ADM were differentially distributed between implantation sites and inter-implantation sites of the pregnant uterus. The day on which vaginal sperm were found was taken to be pregnancy day 1. The Adm mRNA levels in the implantation sites of the uteri in mid- (day 12) and late pregnancy (day 17) were more than 10-fold higher than those in nonpregnancy, pre-implantation (day 3) or early (day 7) pregnancy. ADM was localized in the endometrial stroma with increased immunoreactivity from nonpregnancy to pregnancy. The ADM level and the mRNA levels of Adm, Crlr, Ramp2 and Ramp3 in the corpus luteum all increased in late pregnancy compared with early pregnancy. The gene expression of Adm and it receptor components and intense immunostaining of ADM were also found in the oviduct during pregnancy.Conclusions: The gene expressions levels of Adm and its receptor components - Crlr, Ramp1, Ramp2 and Ramp3, and ADM peptide concentration exhibited a spatio-temporal pattern in the rat female reproductive system during gestation and this suggests that ADM may play important roles in gestation. © 2010 Li et al; licensee BioMed Central Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/142307
ISSN
2015 Impact Factor: 2.147
2015 SCImago Journal Rankings: 0.919
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
Research Grants Council (RGC) of the Hong Kong Special Administrative Region, ChinaHKU 7736/07M
Funding Information:

This study was substantially supported by a grant from the Research Grants Council (RGC) of the Hong Kong Special Administrative Region, China (HKU 7736/07M).

References

 

DC FieldValueLanguage
dc.contributor.authorLi, Len_HK
dc.contributor.authorTang, Fen_HK
dc.contributor.authorO, WSen_HK
dc.date.accessioned2011-10-28T02:42:37Z-
dc.date.available2011-10-28T02:42:37Z-
dc.date.issued2010en_HK
dc.identifier.citationReproductive Biology And Endocrinology, 2010, v. 8en_HK
dc.identifier.issn1477-7827en_HK
dc.identifier.urihttp://hdl.handle.net/10722/142307-
dc.description.abstractBackground: Adrenomedullin (ADM), a novel vasorelaxant peptide, was found in human/rat ovaries and uteri. Plasma ADM level increases in pregnant women and pregnant rats.Methods: The gene expression levels of Adm and its receptor components - Crlr, Ramp1, Ramp2 and Ramp3, the ADM peptide concentration and localization in the rat female reproductive system during gestation were studied by real-time RT-PCR, EIA and immunohistochemical techniques.Results: The mRNAs of Adm and its receptor component and ADM were differentially distributed between implantation sites and inter-implantation sites of the pregnant uterus. The day on which vaginal sperm were found was taken to be pregnancy day 1. The Adm mRNA levels in the implantation sites of the uteri in mid- (day 12) and late pregnancy (day 17) were more than 10-fold higher than those in nonpregnancy, pre-implantation (day 3) or early (day 7) pregnancy. ADM was localized in the endometrial stroma with increased immunoreactivity from nonpregnancy to pregnancy. The ADM level and the mRNA levels of Adm, Crlr, Ramp2 and Ramp3 in the corpus luteum all increased in late pregnancy compared with early pregnancy. The gene expression of Adm and it receptor components and intense immunostaining of ADM were also found in the oviduct during pregnancy.Conclusions: The gene expressions levels of Adm and its receptor components - Crlr, Ramp1, Ramp2 and Ramp3, and ADM peptide concentration exhibited a spatio-temporal pattern in the rat female reproductive system during gestation and this suggests that ADM may play important roles in gestation. © 2010 Li et al; licensee BioMed Central Ltd.en_HK
dc.languageengen_US
dc.publisherBioMed Central Ltd. The Journal's web site is located at http://www.rbej.com/homeen_HK
dc.relation.ispartofReproductive Biology and Endocrinologyen_HK
dc.rightsReproductive Biology and Endocrinology. Copyright © BioMed Central Ltd.-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshAdrenomedullin - genetics - metabolism-
dc.subject.meshCalcitonin Receptor-Like Protein - genetics - metabolism-
dc.subject.meshGenitalia, Female - metabolism-
dc.subject.meshPregnancy, Animal - genetics - metabolism-
dc.subject.meshReceptors, Calcitonin - genetics - metabolism-
dc.titleCoexpression of adrenomedullin and its receptor component proteins in the reproductive system of the rat during gestationen_HK
dc.typeArticleen_HK
dc.identifier.emailTang, F: ftang@hkucc.hku.hken_HK
dc.identifier.emailO, WS: owaisum@hkucc.hku.hken_HK
dc.identifier.authorityTang, F=rp00327en_HK
dc.identifier.authorityO, WS=rp00315en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1186/1477-7827-8-130en_HK
dc.identifier.pmid21034462-
dc.identifier.pmcidPMC2984462-
dc.identifier.scopuseid_2-s2.0-78549290634en_HK
dc.identifier.hkuros184413en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-78549290634&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume8en_HK
dc.identifier.isiWOS:000284267900001-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLi, L=36071951300en_HK
dc.identifier.scopusauthoridTang, F=7201979770en_HK
dc.identifier.scopusauthoridO, WS=6701729369en_HK
dc.identifier.citeulike8167785-

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