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Article: Association of matrix metalloproteinase (MMP-1, MMP-3 and MMP-9) and cyclooxygenase-2 gene polymorphisms and their proteins with chronic periodontitis

TitleAssociation of matrix metalloproteinase (MMP-1, MMP-3 and MMP-9) and cyclooxygenase-2 gene polymorphisms and their proteins with chronic periodontitis
Authors
KeywordsChronic periodontitis
Cyclooxygenase-2
Metalloproteinases
Polymorphism
Vascular Endothelial Growth Factor
Issue Date2011
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/archoralbio
Citation
Archives Of Oral Biology, 2011, v. 56 n. 10, p. 1081-1090 How to Cite?
AbstractAims: The objective of this study was to investigate the association amongst the single nucleotide polymorphisms of genes encoding for matrix metalloproteinase (MMP) 1, 3, 9 and cyclooxygenase-2 (COX-2) of subjects. Protein production of MMPs, COX-2 and Vascular Endothelial Growth Factor (VEGF) were also investigated. Methods: 280 chronic periodontitis patients and 250 periodontitis-free subjects were selected. DNA was extracted from blood samples of all patients, the polymorphic sites of the genes that encode for metalloproteinases and cyclooxygenase-2 were amplified using PCR, and digested with restriction enzymes. ELISA was used to determine the protein production of MMPs, COX-2 and VEGF. Results: The mean probing depth (PD) was 5.4 mm and the clinical attachment loss (CAL) was 6.4 mm in patients group with at least 2 years history. 2G/2G genotype of MMP-1, the periodontitis patients presented frequency of 28% and the control only showed 3%. 5A/5A genotype of MMP-3, the periodontitis patients presented higher frequency of 55% than the control 40%. C/C of genotype MMP-9, the periodontitis patients presented higher frequency of 51% than the control 17%. C/C of genotype COX-2, the periodontitis patients demonstrated 28% frequency and the control was 3%. ELISA analysis determined a significant difference (p < 0.001) in protein production between patient and control samples for the bio-markers. 12 cases with suspicious genotype of MMPs and in COX-2 showed the serum level was the highest value between other C/C genotype. Conclusions: Combine genotype and serum expression of inflammatory mediators that may be a good bio-marker for diagnosis and prognosis of the periodontitis. © 2011 Elsevier Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/142293
ISSN
2015 Impact Factor: 1.733
2015 SCImago Journal Rankings: 0.713
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLoo, WTYen_HK
dc.contributor.authorWang, Men_HK
dc.contributor.authorJin, LJen_HK
dc.contributor.authorCheung, MNBen_HK
dc.contributor.authorLi, GRen_HK
dc.date.accessioned2011-10-28T02:42:11Z-
dc.date.available2011-10-28T02:42:11Z-
dc.date.issued2011en_HK
dc.identifier.citationArchives Of Oral Biology, 2011, v. 56 n. 10, p. 1081-1090en_HK
dc.identifier.issn0003-9969en_HK
dc.identifier.urihttp://hdl.handle.net/10722/142293-
dc.description.abstractAims: The objective of this study was to investigate the association amongst the single nucleotide polymorphisms of genes encoding for matrix metalloproteinase (MMP) 1, 3, 9 and cyclooxygenase-2 (COX-2) of subjects. Protein production of MMPs, COX-2 and Vascular Endothelial Growth Factor (VEGF) were also investigated. Methods: 280 chronic periodontitis patients and 250 periodontitis-free subjects were selected. DNA was extracted from blood samples of all patients, the polymorphic sites of the genes that encode for metalloproteinases and cyclooxygenase-2 were amplified using PCR, and digested with restriction enzymes. ELISA was used to determine the protein production of MMPs, COX-2 and VEGF. Results: The mean probing depth (PD) was 5.4 mm and the clinical attachment loss (CAL) was 6.4 mm in patients group with at least 2 years history. 2G/2G genotype of MMP-1, the periodontitis patients presented frequency of 28% and the control only showed 3%. 5A/5A genotype of MMP-3, the periodontitis patients presented higher frequency of 55% than the control 40%. C/C of genotype MMP-9, the periodontitis patients presented higher frequency of 51% than the control 17%. C/C of genotype COX-2, the periodontitis patients demonstrated 28% frequency and the control was 3%. ELISA analysis determined a significant difference (p < 0.001) in protein production between patient and control samples for the bio-markers. 12 cases with suspicious genotype of MMPs and in COX-2 showed the serum level was the highest value between other C/C genotype. Conclusions: Combine genotype and serum expression of inflammatory mediators that may be a good bio-marker for diagnosis and prognosis of the periodontitis. © 2011 Elsevier Ltd.en_HK
dc.languageengen_US
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/archoralbioen_HK
dc.relation.ispartofArchives of Oral Biologyen_HK
dc.subjectChronic periodontitisen_HK
dc.subjectCyclooxygenase-2en_HK
dc.subjectMetalloproteinasesen_HK
dc.subjectPolymorphismen_HK
dc.subjectVascular Endothelial Growth Factoren_HK
dc.subject.meshChronic Periodontitis - classification - enzymology - genetics-
dc.subject.meshCyclooxygenase 2 - blood - genetics-
dc.subject.meshMatrix Metalloproteinase 1 - blood - genetics-
dc.subject.meshMatrix Metalloproteinase 3 - blood - genetics-
dc.subject.meshMatrix Metalloproteinase 9 - blood - genetics-
dc.titleAssociation of matrix metalloproteinase (MMP-1, MMP-3 and MMP-9) and cyclooxygenase-2 gene polymorphisms and their proteins with chronic periodontitisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0003-9969&volume=56&spage=1081&epage=1090&date=2011&atitle=Association+of+matrix+metalloproteinase+(MMP-1,+MMP-3+and+MMP-9)+and+cyclooxygenase-2+gene+polymorphisms+and+their+proteins+with+chronic+periodontitisen_US
dc.identifier.emailJin, LJ:ljjin@hkucc.hku.hken_HK
dc.identifier.emailLi, GR:grli@hkucc.hku.hken_HK
dc.identifier.authorityJin, LJ=rp00028en_HK
dc.identifier.authorityLi, GR=rp00476en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.archoralbio.2011.03.011en_HK
dc.identifier.pmid21481333-
dc.identifier.scopuseid_2-s2.0-80053574088en_HK
dc.identifier.hkuros197652en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80053574088&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume56en_HK
dc.identifier.issue10en_HK
dc.identifier.spage1081en_HK
dc.identifier.epage1090en_HK
dc.identifier.isiWOS:000296112200020-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLoo, WTY=7003567474en_HK
dc.identifier.scopusauthoridWang, M=36080215600en_HK
dc.identifier.scopusauthoridJin, LJ=7403328850en_HK
dc.identifier.scopusauthoridCheung, MNB=7201897548en_HK
dc.identifier.scopusauthoridLi, GR=7408462932en_HK
dc.identifier.citeulike9188371-

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