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Conference Paper: Metergoline-induced cell death in Candida krusei

TitleMetergoline-induced cell death in Candida krusei
Authors
KeywordsCandida krusei
DNA fragmentation
Metergoline
Mitochondrial membrane potential
Reactive oxygen species
Issue Date2011
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/720691/description#description
Citation
The 2nd International Conference on Drug Discovery and Therapy (ICDDT 2010), Dubai, UAE, 1-4 February 2010. In Fungal Biology, 2011, v. 115 n. 3, p. 302-309 How to Cite?
AbstractMetergoline possesses potent antifungal activity against Candida krusei, a notorious yeast species that is inherently resistant to the common antifungal agents. In an attempt to elucidate the action mechanisms of metergoline, the present study was designed to investigate its effects on a number of classical markers of apoptosis in C. krusei. The results showed that transient exposure (2. h) to metergoline led to a massive intracellular accumulation of reactive oxygen species (ROS) and depolarization of mitochondrial membrane potential in a concentration-dependent fashion. Analyses of the treated fungal cells after prolonged incubation (12. h) with metergoline by flow cytometry and fluorescence microscopy clearly demonstrated phosphatidylserine externalization, the presence of terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labelling-positive cells and fungal cells undergoing necrosis. Taken together, our data provide evidence that metergoline elicited cell death process in C. krusei through elevation of the intracellular ROS level and perturbation of mitochondrial homeostasis, followed by damage of nucleus and eventual cell demise. © 2011 The British Mycological Society.
Persistent Identifierhttp://hdl.handle.net/10722/142280
ISSN
2015 Impact Factor: 2.244
2015 SCImago Journal Rankings: 0.969
ISI Accession Number ID
Funding AgencyGrant Number
Research Fund for the Control of Infectious Diseases
Health Bureau of the Government of the HKSARRFCID 08070142
Funding Information:

This work was partially supported by the Research Fund for the Control of Infectious Diseases, the Food and Health Bureau of the Government of the HKSAR (RFCID 08070142 to PWKT). We are grateful to Mr Chan Chi Lung and Ms Choi Pui Wah for their technical assistance.

References

 

DC FieldValueLanguage
dc.contributor.authorKang, Ken_HK
dc.contributor.authorWong, KSen_HK
dc.contributor.authorFong, WPen_HK
dc.contributor.authorTsang, PWKen_HK
dc.date.accessioned2011-10-28T02:42:03Z-
dc.date.available2011-10-28T02:42:03Z-
dc.date.issued2011en_HK
dc.identifier.citationThe 2nd International Conference on Drug Discovery and Therapy (ICDDT 2010), Dubai, UAE, 1-4 February 2010. In Fungal Biology, 2011, v. 115 n. 3, p. 302-309en_HK
dc.identifier.issn1878-6146en_HK
dc.identifier.urihttp://hdl.handle.net/10722/142280-
dc.description.abstractMetergoline possesses potent antifungal activity against Candida krusei, a notorious yeast species that is inherently resistant to the common antifungal agents. In an attempt to elucidate the action mechanisms of metergoline, the present study was designed to investigate its effects on a number of classical markers of apoptosis in C. krusei. The results showed that transient exposure (2. h) to metergoline led to a massive intracellular accumulation of reactive oxygen species (ROS) and depolarization of mitochondrial membrane potential in a concentration-dependent fashion. Analyses of the treated fungal cells after prolonged incubation (12. h) with metergoline by flow cytometry and fluorescence microscopy clearly demonstrated phosphatidylserine externalization, the presence of terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labelling-positive cells and fungal cells undergoing necrosis. Taken together, our data provide evidence that metergoline elicited cell death process in C. krusei through elevation of the intracellular ROS level and perturbation of mitochondrial homeostasis, followed by damage of nucleus and eventual cell demise. © 2011 The British Mycological Society.en_HK
dc.languageengen_US
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/720691/description#descriptionen_HK
dc.relation.ispartofFungal Biologyen_HK
dc.subjectCandida kruseien_HK
dc.subjectDNA fragmentationen_HK
dc.subjectMetergolineen_HK
dc.subjectMitochondrial membrane potentialen_HK
dc.subjectReactive oxygen speciesen_HK
dc.subject.meshAntifungal Agents - pharmacology-
dc.subject.meshApoptosis - drug effects-
dc.subject.meshCandida - classification - drug effects - ultrastructure-
dc.subject.meshMetergoline - pharmacology-
dc.subject.meshNecrosis - chemically induced-
dc.titleMetergoline-induced cell death in Candida kruseien_HK
dc.typeConference_Paperen_HK
dc.identifier.emailTsang, PWK:pwktsang@hku.hken_HK
dc.identifier.authorityTsang, PWK=rp01388en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.funbio.2011.01.001en_HK
dc.identifier.pmid21354537-
dc.identifier.scopuseid_2-s2.0-79952009346en_HK
dc.identifier.hkuros184610en_US
dc.identifier.hkuros175347-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79952009346&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume115en_HK
dc.identifier.issue3en_HK
dc.identifier.spage302en_HK
dc.identifier.epage309en_HK
dc.identifier.isiWOS:000289023800012-
dc.publisher.placeUnited Kingdomen_HK
dc.description.otherThe 2nd International Conference on Drug Discovery and Therapy (ICDDT 2010), Dubai, UAE, 1-4 February 2010. In Fungal Biology, 2011, v. 115 n. 3, p. 302-309-
dc.identifier.scopusauthoridKang, K=36544735400en_HK
dc.identifier.scopusauthoridWong, KS=35191126000en_HK
dc.identifier.scopusauthoridFong, WP=7102816006en_HK
dc.identifier.scopusauthoridTsang, PWK=8334953500en_HK

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