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Article: Expression of hypoxia-inducible factor-1α in human periodontal tissue

TitleExpression of hypoxia-inducible factor-1α in human periodontal tissue
Authors
KeywordsCell hypoxia
Chronic periodontitis
Hypoxia-inducible factor 1-α subunit
Tumor necrosis factor-alpha
Vascular endothelial growth factor A
Issue Date2011
PublisherAmerican Academy of Periodontology. The Journal's web site is located at http://www.perio.org
Citation
Journal Of Periodontology, 2011, v. 82 n. 1, p. 136-141 How to Cite?
AbstractBackground: Hypoxia-inducible factor (HIF)-1 is a key transcription factor responding to hypoxia. It is composed of an oxygen-sensitive α subunit (HIF-1α) and a constitutively expressed β subunit. Increasing evidence indicates an essential role for HIF-1α in infection and immunity. Because inflamed periodontium is thought to be hypoxic, we hypothesize that HIF-1α is expressed and related to its upstream regulator tumor necrosis factor (TNF)-a and downstream effecter vascular endothelial growth factor (VEGF). Methods: Human gingival biopsies were collected from advanced periodontitis sites and clinically healthy sites, and immunohistochemically examined for HIF-1α and VEGF peptides. Themessenger ribonucleic acid (mRNA) and protein levels of HIF-1α, VEGF, and TNF-α in the biopsies were then assessed by reverse transcription polymerase chain reaction and Western blotting. Results: HIF-1α-positive immunoreactivity was detected in the nuclei of epithelial and endothelial cells. In periodontal pockets, there was a marked increase in the proportion of fibroblast-like cells and leukocyte-like cells expressing HIF-1α. Protein levels of HIF-1α, VEGF, and TNF-α were significantly higher in periodontal pockets than in control gingival samples. The mRNA expression of VEGF and TNF-α was also increased in periodontal pockets. Conclusion: HIF-1α is expressed in healthy and diseased periodontium andmay be related to TNF-α and VEGF function during periodontitis.
Persistent Identifierhttp://hdl.handle.net/10722/142267
ISSN
2014 Impact Factor: 2.706
2014 SCImago Journal Rankings: 0.918
ISI Accession Number ID
Funding AgencyGrant Number
University of Hong Kong10400006
10400878
Research Grants Council of the Hong Kong SAR, China (SFPBR)200711159017
Funding Information:

The authors acknowledge W.B. Wong, K.M. Leung (Li Ka Shing Faculty of Medicine, the University of Hong Kong) for technical assistance, and Trevor Lane (Faculty of Dentistry, the University of Hong Kong) for manuscript editing. This project was supported by the University of Hong Kong Research and Conference Grants (10400006 and 10400878) and Research Grants Council of the Hong Kong SAR, China (SFPBR 200711159017). King-Tung Ng and Jing-Ping Li contributed equally to this work. The authors report no conflicts of interest related to this study.

References

 

DC FieldValueLanguage
dc.contributor.authorNg, KTen_HK
dc.contributor.authorLi, JPen_HK
dc.contributor.authorNg, KMen_HK
dc.contributor.authorTipoe, GLen_HK
dc.contributor.authorLeung, WKen_HK
dc.contributor.authorFung, MLen_HK
dc.date.accessioned2011-10-28T02:41:57Z-
dc.date.available2011-10-28T02:41:57Z-
dc.date.issued2011en_HK
dc.identifier.citationJournal Of Periodontology, 2011, v. 82 n. 1, p. 136-141en_HK
dc.identifier.issn0022-3492en_HK
dc.identifier.urihttp://hdl.handle.net/10722/142267-
dc.description.abstractBackground: Hypoxia-inducible factor (HIF)-1 is a key transcription factor responding to hypoxia. It is composed of an oxygen-sensitive α subunit (HIF-1α) and a constitutively expressed β subunit. Increasing evidence indicates an essential role for HIF-1α in infection and immunity. Because inflamed periodontium is thought to be hypoxic, we hypothesize that HIF-1α is expressed and related to its upstream regulator tumor necrosis factor (TNF)-a and downstream effecter vascular endothelial growth factor (VEGF). Methods: Human gingival biopsies were collected from advanced periodontitis sites and clinically healthy sites, and immunohistochemically examined for HIF-1α and VEGF peptides. Themessenger ribonucleic acid (mRNA) and protein levels of HIF-1α, VEGF, and TNF-α in the biopsies were then assessed by reverse transcription polymerase chain reaction and Western blotting. Results: HIF-1α-positive immunoreactivity was detected in the nuclei of epithelial and endothelial cells. In periodontal pockets, there was a marked increase in the proportion of fibroblast-like cells and leukocyte-like cells expressing HIF-1α. Protein levels of HIF-1α, VEGF, and TNF-α were significantly higher in periodontal pockets than in control gingival samples. The mRNA expression of VEGF and TNF-α was also increased in periodontal pockets. Conclusion: HIF-1α is expressed in healthy and diseased periodontium andmay be related to TNF-α and VEGF function during periodontitis.en_HK
dc.languageengen_US
dc.publisherAmerican Academy of Periodontology. The Journal's web site is located at http://www.perio.orgen_HK
dc.relation.ispartofJournal of Periodontologyen_HK
dc.subjectCell hypoxiaen_HK
dc.subjectChronic periodontitisen_HK
dc.subjectHypoxia-inducible factor 1-α subuniten_HK
dc.subjectTumor necrosis factor-alphaen_HK
dc.subjectVascular endothelial growth factor Aen_HK
dc.subject.meshChronic Periodontitis - metabolism - pathology-
dc.subject.meshEndothelial Cells - metabolism - pathology-
dc.subject.meshEpithelial Cells - metabolism - pathology-
dc.titleExpression of hypoxia-inducible factor-1α in human periodontal tissueen_HK
dc.typeArticleen_HK
dc.identifier.emailNg, KM: skykmng@hkucc.hku.hken_HK
dc.identifier.emailTipoe, GL: tgeorge@hkucc.hku.hken_HK
dc.identifier.emailLeung, WK: ewkleung@hkucc.hku.hken_HK
dc.identifier.emailFung, ML: fungml@hkucc.hku.hken_HK
dc.identifier.authorityNg, KM=rp01670en_HK
dc.identifier.authorityTipoe, GL=rp00371en_HK
dc.identifier.authorityLeung, WK=rp00019en_HK
dc.identifier.authorityFung, ML=rp00433en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1902/jop.2010.100100en_HK
dc.identifier.pmid21043802en_HK
dc.identifier.scopuseid_2-s2.0-79251624676en_HK
dc.identifier.hkuros184271en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79251624676&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume82en_HK
dc.identifier.issue1en_HK
dc.identifier.spage136en_HK
dc.identifier.epage141en_HK
dc.identifier.eissn1943-3670-
dc.identifier.isiWOS:000286347900016-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridNg, KT=40561571800en_HK
dc.identifier.scopusauthoridLi, JP=40561311800en_HK
dc.identifier.scopusauthoridNg, KM=25122990200en_HK
dc.identifier.scopusauthoridTipoe, GL=7003550610en_HK
dc.identifier.scopusauthoridLeung, WK=25224691800en_HK
dc.identifier.scopusauthoridFung, ML=7101955092en_HK

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