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Article: Expression of hypoxia-inducible factor-1α in human periodontal tissue
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TitleExpression of hypoxia-inducible factor-1α in human periodontal tissue
 
AuthorsNg, KT1
Li, JP2
Ng, KM1
Tipoe, GL1
Leung, WK1 2
Fung, ML1
 
KeywordsCell hypoxia
Chronic periodontitis
Hypoxia-inducible factor 1-α subunit
Tumor necrosis factor-alpha
Vascular endothelial growth factor A
 
Issue Date2011
 
PublisherAmerican Academy of Periodontology. The Journal's web site is located at http://www.perio.org
 
CitationJournal Of Periodontology, 2011, v. 82 n. 1, p. 136-141 [How to Cite?]
DOI: http://dx.doi.org/10.1902/jop.2010.100100
 
AbstractBackground: Hypoxia-inducible factor (HIF)-1 is a key transcription factor responding to hypoxia. It is composed of an oxygen-sensitive α subunit (HIF-1α) and a constitutively expressed β subunit. Increasing evidence indicates an essential role for HIF-1α in infection and immunity. Because inflamed periodontium is thought to be hypoxic, we hypothesize that HIF-1α is expressed and related to its upstream regulator tumor necrosis factor (TNF)-a and downstream effecter vascular endothelial growth factor (VEGF). Methods: Human gingival biopsies were collected from advanced periodontitis sites and clinically healthy sites, and immunohistochemically examined for HIF-1α and VEGF peptides. Themessenger ribonucleic acid (mRNA) and protein levels of HIF-1α, VEGF, and TNF-α in the biopsies were then assessed by reverse transcription polymerase chain reaction and Western blotting. Results: HIF-1α-positive immunoreactivity was detected in the nuclei of epithelial and endothelial cells. In periodontal pockets, there was a marked increase in the proportion of fibroblast-like cells and leukocyte-like cells expressing HIF-1α. Protein levels of HIF-1α, VEGF, and TNF-α were significantly higher in periodontal pockets than in control gingival samples. The mRNA expression of VEGF and TNF-α was also increased in periodontal pockets. Conclusion: HIF-1α is expressed in healthy and diseased periodontium andmay be related to TNF-α and VEGF function during periodontitis.
 
ISSN0022-3492
2013 Impact Factor: 2.565
2013 SCImago Journal Rankings: 1.143
 
DOIhttp://dx.doi.org/10.1902/jop.2010.100100
 
ISI Accession Number IDWOS:000286347900016
Funding AgencyGrant Number
University of Hong Kong10400006
10400878
Research Grants Council of the Hong Kong SAR, China (SFPBR)200711159017
Funding Information:

The authors acknowledge W.B. Wong, K.M. Leung (Li Ka Shing Faculty of Medicine, the University of Hong Kong) for technical assistance, and Trevor Lane (Faculty of Dentistry, the University of Hong Kong) for manuscript editing. This project was supported by the University of Hong Kong Research and Conference Grants (10400006 and 10400878) and Research Grants Council of the Hong Kong SAR, China (SFPBR 200711159017). King-Tung Ng and Jing-Ping Li contributed equally to this work. The authors report no conflicts of interest related to this study.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorNg, KT
 
dc.contributor.authorLi, JP
 
dc.contributor.authorNg, KM
 
dc.contributor.authorTipoe, GL
 
dc.contributor.authorLeung, WK
 
dc.contributor.authorFung, ML
 
dc.date.accessioned2011-10-28T02:41:57Z
 
dc.date.available2011-10-28T02:41:57Z
 
dc.date.issued2011
 
dc.description.abstractBackground: Hypoxia-inducible factor (HIF)-1 is a key transcription factor responding to hypoxia. It is composed of an oxygen-sensitive α subunit (HIF-1α) and a constitutively expressed β subunit. Increasing evidence indicates an essential role for HIF-1α in infection and immunity. Because inflamed periodontium is thought to be hypoxic, we hypothesize that HIF-1α is expressed and related to its upstream regulator tumor necrosis factor (TNF)-a and downstream effecter vascular endothelial growth factor (VEGF). Methods: Human gingival biopsies were collected from advanced periodontitis sites and clinically healthy sites, and immunohistochemically examined for HIF-1α and VEGF peptides. Themessenger ribonucleic acid (mRNA) and protein levels of HIF-1α, VEGF, and TNF-α in the biopsies were then assessed by reverse transcription polymerase chain reaction and Western blotting. Results: HIF-1α-positive immunoreactivity was detected in the nuclei of epithelial and endothelial cells. In periodontal pockets, there was a marked increase in the proportion of fibroblast-like cells and leukocyte-like cells expressing HIF-1α. Protein levels of HIF-1α, VEGF, and TNF-α were significantly higher in periodontal pockets than in control gingival samples. The mRNA expression of VEGF and TNF-α was also increased in periodontal pockets. Conclusion: HIF-1α is expressed in healthy and diseased periodontium andmay be related to TNF-α and VEGF function during periodontitis.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationJournal Of Periodontology, 2011, v. 82 n. 1, p. 136-141 [How to Cite?]
DOI: http://dx.doi.org/10.1902/jop.2010.100100
 
dc.identifier.doihttp://dx.doi.org/10.1902/jop.2010.100100
 
dc.identifier.eissn1943-3670
 
dc.identifier.epage141
 
dc.identifier.hkuros184271
 
dc.identifier.isiWOS:000286347900016
Funding AgencyGrant Number
University of Hong Kong10400006
10400878
Research Grants Council of the Hong Kong SAR, China (SFPBR)200711159017
Funding Information:

The authors acknowledge W.B. Wong, K.M. Leung (Li Ka Shing Faculty of Medicine, the University of Hong Kong) for technical assistance, and Trevor Lane (Faculty of Dentistry, the University of Hong Kong) for manuscript editing. This project was supported by the University of Hong Kong Research and Conference Grants (10400006 and 10400878) and Research Grants Council of the Hong Kong SAR, China (SFPBR 200711159017). King-Tung Ng and Jing-Ping Li contributed equally to this work. The authors report no conflicts of interest related to this study.

 
dc.identifier.issn0022-3492
2013 Impact Factor: 2.565
2013 SCImago Journal Rankings: 1.143
 
dc.identifier.issue1
 
dc.identifier.pmid21043802
 
dc.identifier.scopuseid_2-s2.0-79251624676
 
dc.identifier.spage136
 
dc.identifier.urihttp://hdl.handle.net/10722/142267
 
dc.identifier.volume82
 
dc.languageeng
 
dc.publisherAmerican Academy of Periodontology. The Journal's web site is located at http://www.perio.org
 
dc.publisher.placeUnited States
 
dc.relation.ispartofJournal of Periodontology
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshChronic Periodontitis - metabolism - pathology
 
dc.subject.meshEndothelial Cells - metabolism - pathology
 
dc.subject.meshEpithelial Cells - metabolism - pathology
 
dc.subjectCell hypoxia
 
dc.subjectChronic periodontitis
 
dc.subjectHypoxia-inducible factor 1-α subunit
 
dc.subjectTumor necrosis factor-alpha
 
dc.subjectVascular endothelial growth factor A
 
dc.titleExpression of hypoxia-inducible factor-1α in human periodontal tissue
 
dc.typeArticle
 
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<contributor.author>Li, JP</contributor.author>
<contributor.author>Ng, KM</contributor.author>
<contributor.author>Tipoe, GL</contributor.author>
<contributor.author>Leung, WK</contributor.author>
<contributor.author>Fung, ML</contributor.author>
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<description.abstract>Background: Hypoxia-inducible factor (HIF)-1 is a key transcription factor responding to hypoxia. It is composed of an oxygen-sensitive &#945; subunit (HIF-1&#945;) and a constitutively expressed &#946; subunit. Increasing evidence indicates an essential role for HIF-1&#945; in infection and immunity. Because inflamed periodontium is thought to be hypoxic, we hypothesize that HIF-1&#945; is expressed and related to its upstream regulator tumor necrosis factor (TNF)-a and downstream effecter vascular endothelial growth factor (VEGF). Methods: Human gingival biopsies were collected from advanced periodontitis sites and clinically healthy sites, and immunohistochemically examined for HIF-1&#945; and VEGF peptides. Themessenger ribonucleic acid (mRNA) and protein levels of HIF-1&#945;, VEGF, and TNF-&#945; in the biopsies were then assessed by reverse transcription polymerase chain reaction and Western blotting. Results: HIF-1&#945;-positive immunoreactivity was detected in the nuclei of epithelial and endothelial cells. In periodontal pockets, there was a marked increase in the proportion of fibroblast-like cells and leukocyte-like cells expressing HIF-1&#945;. Protein levels of HIF-1&#945;, VEGF, and TNF-&#945; were significantly higher in periodontal pockets than in control gingival samples. The mRNA expression of VEGF and TNF-&#945; was also increased in periodontal pockets. Conclusion: HIF-1&#945; is expressed in healthy and diseased periodontium andmay be related to TNF-&#945; and VEGF function during periodontitis.</description.abstract>
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<subject>Chronic periodontitis</subject>
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Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. The University of Hong Kong