Article: Expression of hypoxia-inducible factor-1α in human periodontal tissue
| Title | Expression of hypoxia-inducible factor-1α in human periodontal tissue | ||||||
|---|---|---|---|---|---|---|---|
| Authors | Ng, KT1 Li, JP2 Ng, KM1 Tipoe, GL1 Leung, WK1 2 Fung, ML1 | ||||||
| Keywords | Cell hypoxia Chronic periodontitis Hypoxia-inducible factor 1-α subunit Tumor necrosis factor-alpha Vascular endothelial growth factor A | ||||||
| Issue Date | 2011 | ||||||
| Publisher | American Academy of Periodontology. The Journal's web site is located at http://www.perio.org | ||||||
| Citation | Journal Of Periodontology, 2011, v. 82 n. 1, p. 136-141 [How to Cite?] DOI: http://dx.doi.org/10.1902/jop.2010.100100 | ||||||
| Abstract | Background: Hypoxia-inducible factor (HIF)-1 is a key transcription factor responding to hypoxia. It is composed of an oxygen-sensitive α subunit (HIF-1α) and a constitutively expressed β subunit. Increasing evidence indicates an essential role for HIF-1α in infection and immunity. Because inflamed periodontium is thought to be hypoxic, we hypothesize that HIF-1α is expressed and related to its upstream regulator tumor necrosis factor (TNF)-a and downstream effecter vascular endothelial growth factor (VEGF). Methods: Human gingival biopsies were collected from advanced periodontitis sites and clinically healthy sites, and immunohistochemically examined for HIF-1α and VEGF peptides. Themessenger ribonucleic acid (mRNA) and protein levels of HIF-1α, VEGF, and TNF-α in the biopsies were then assessed by reverse transcription polymerase chain reaction and Western blotting. Results: HIF-1α-positive immunoreactivity was detected in the nuclei of epithelial and endothelial cells. In periodontal pockets, there was a marked increase in the proportion of fibroblast-like cells and leukocyte-like cells expressing HIF-1α. Protein levels of HIF-1α, VEGF, and TNF-α were significantly higher in periodontal pockets than in control gingival samples. The mRNA expression of VEGF and TNF-α was also increased in periodontal pockets. Conclusion: HIF-1α is expressed in healthy and diseased periodontium andmay be related to TNF-α and VEGF function during periodontitis. | ||||||
| ISSN | 0022-3492 2011 Impact Factor: 2.602 2011 SCImago Journal Rankings: 0.140 | ||||||
| DOI | http://dx.doi.org/10.1902/jop.2010.100100 | ||||||
| ISI Accession Number ID | WOS:000286347900016
Funding Information: The authors acknowledge W.B. Wong, K.M. Leung (Li Ka Shing Faculty of Medicine, the University of Hong Kong) for technical assistance, and Trevor Lane (Faculty of Dentistry, the University of Hong Kong) for manuscript editing. This project was supported by the University of Hong Kong Research and Conference Grants (10400006 and 10400878) and Research Grants Council of the Hong Kong SAR, China (SFPBR 200711159017). King-Tung Ng and Jing-Ping Li contributed equally to this work. The authors report no conflicts of interest related to this study. | ||||||
| References | References in Scopus | ||||||
| Grants | Mechanistic role of hypoxia-inducible factor-1 in differentiation of embryonic stem cells into cardiomyocytes |
| dc.contributor.author | Ng, KT | ||||||
|---|---|---|---|---|---|---|---|
| dc.contributor.author | Li, JP | ||||||
| dc.contributor.author | Ng, KM | ||||||
| dc.contributor.author | Tipoe, GL | ||||||
| dc.contributor.author | Leung, WK | ||||||
| dc.contributor.author | Fung, ML | ||||||
| dc.date.accessioned | 2011-10-28T02:41:57Z | ||||||
| dc.date.available | 2011-10-28T02:41:57Z | ||||||
| dc.date.issued | 2011 | ||||||
| dc.description.abstract | Background: Hypoxia-inducible factor (HIF)-1 is a key transcription factor responding to hypoxia. It is composed of an oxygen-sensitive α subunit (HIF-1α) and a constitutively expressed β subunit. Increasing evidence indicates an essential role for HIF-1α in infection and immunity. Because inflamed periodontium is thought to be hypoxic, we hypothesize that HIF-1α is expressed and related to its upstream regulator tumor necrosis factor (TNF)-a and downstream effecter vascular endothelial growth factor (VEGF). Methods: Human gingival biopsies were collected from advanced periodontitis sites and clinically healthy sites, and immunohistochemically examined for HIF-1α and VEGF peptides. Themessenger ribonucleic acid (mRNA) and protein levels of HIF-1α, VEGF, and TNF-α in the biopsies were then assessed by reverse transcription polymerase chain reaction and Western blotting. Results: HIF-1α-positive immunoreactivity was detected in the nuclei of epithelial and endothelial cells. In periodontal pockets, there was a marked increase in the proportion of fibroblast-like cells and leukocyte-like cells expressing HIF-1α. Protein levels of HIF-1α, VEGF, and TNF-α were significantly higher in periodontal pockets than in control gingival samples. The mRNA expression of VEGF and TNF-α was also increased in periodontal pockets. Conclusion: HIF-1α is expressed in healthy and diseased periodontium andmay be related to TNF-α and VEGF function during periodontitis. | ||||||
| dc.description.grant | Mechanistic role of hypoxia-inducible factor-1 in differentiation of embryonic stem cells into cardiomyocytes | ||||||
| dc.description.grantcode | 97871 | ||||||
| dc.description.nature | link_to_subscribed_fulltext | ||||||
| dc.identifier.citation | Journal Of Periodontology, 2011, v. 82 n. 1, p. 136-141 [How to Cite?] DOI: http://dx.doi.org/10.1902/jop.2010.100100 | ||||||
| dc.identifier.doi | http://dx.doi.org/10.1902/jop.2010.100100 | ||||||
| dc.identifier.epage | 141 | ||||||
| dc.identifier.hkuros | 184271 | ||||||
| dc.identifier.isi | WOS:000286347900016
Funding Information: The authors acknowledge W.B. Wong, K.M. Leung (Li Ka Shing Faculty of Medicine, the University of Hong Kong) for technical assistance, and Trevor Lane (Faculty of Dentistry, the University of Hong Kong) for manuscript editing. This project was supported by the University of Hong Kong Research and Conference Grants (10400006 and 10400878) and Research Grants Council of the Hong Kong SAR, China (SFPBR 200711159017). King-Tung Ng and Jing-Ping Li contributed equally to this work. The authors report no conflicts of interest related to this study. | ||||||
| dc.identifier.issn | 0022-3492 2011 Impact Factor: 2.602 2011 SCImago Journal Rankings: 0.140 | ||||||
| dc.identifier.issue | 1 | ||||||
| dc.identifier.pmid | 21043802 | ||||||
| dc.identifier.scopus | eid_2-s2.0-79251624676 | ||||||
| dc.identifier.spage | 136 | ||||||
| dc.identifier.uri | http://hdl.handle.net/10722/142267 | ||||||
| dc.identifier.volume | 82 | ||||||
| dc.language | eng | ||||||
| dc.publisher | American Academy of Periodontology. The Journal's web site is located at http://www.perio.org | ||||||
| dc.publisher.place | United States | ||||||
| dc.relation.ispartof | Journal of Periodontology | ||||||
| dc.relation.references | References in Scopus | ||||||
| dc.subject.mesh | Chronic Periodontitis - metabolism - pathology | ||||||
| dc.subject.mesh | Endothelial Cells - metabolism - pathology | ||||||
| dc.subject.mesh | Epithelial Cells - metabolism - pathology | ||||||
| dc.subject | Cell hypoxia | ||||||
| dc.subject | Chronic periodontitis | ||||||
| dc.subject | Hypoxia-inducible factor 1-α subunit | ||||||
| dc.subject | Tumor necrosis factor-alpha | ||||||
| dc.subject | Vascular endothelial growth factor A | ||||||
| dc.title | Expression of hypoxia-inducible factor-1α in human periodontal tissue | ||||||
| dc.type | Article |
Author Affiliations
- The University of Hong Kong Li Ka Shing Faculty of Medicine
- The University of Hong Kong