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Article: Enhanced cell sorting and manipulation with combined optical tweezer and microfluidic chip technologies

TitleEnhanced cell sorting and manipulation with combined optical tweezer and microfluidic chip technologies
Authors
Issue Date2011
PublisherRoyal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/loc
Citation
Lab On A Chip - Miniaturisation For Chemistry And Biology, 2011, v. 11 n. 21, p. 3656-3662 How to Cite?
AbstractSorting (or isolation) and manipulation of rare cells with high recovery rate and purity are of critical importance to a wide range of physiological applications. In the current paper, we report on a generic single cell manipulation tool that integrates optical tweezers and microfluidic chip technologies for handling small cell population sorting with high accuracy. The laminar flow nature of microfluidics enables the targeted cells to be focused on a desired area for cell isolation. To recognize the target cells, we develop an image processing methodology with a recognition capability of multiple features, e.g., cell size and fluorescence label. The target cells can be moved precisely by optical tweezers to the desired destination in a noninvasive manner. The unique advantages of this sorter are its high recovery rate and purity in small cell population sorting. The design is based on dynamic fluid and dynamic light pattern, in which single as well as multiple laser traps are employed for cell transportation, and a recognition capability of multiple cell features. Experiments of sorting yeast cells and human embryonic stem cells are performed to demonstrate the effectiveness of the proposed cell sorting approach. © The Royal Society of Chemistry 2011.
Persistent Identifierhttp://hdl.handle.net/10722/142250
ISSN
2015 Impact Factor: 5.586
2015 SCImago Journal Rankings: 2.373
ISI Accession Number ID
Funding AgencyGrant Number
Research Grants Council of the Hong Kong Special Administrative Region, ChinaCityU 120310
UGCSEG_CityU 01
Theme-based Research Scheme ProjectT13-706/11
City University of Hong Kong7002715
9667034
Funding Information:

This work was supported by grants from the Research Grants Council of the Hong Kong Special Administrative Region, China [Reference no. CityU 120310], UGC Special Equipment Grant [SEG_CityU 01], Theme-based Research Scheme Project (T13-706/11), and City University of Hong Kong (Reference no. 7002715 and 9667034).

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorWang, Xen_HK
dc.contributor.authorChen, Sen_HK
dc.contributor.authorKong, Men_HK
dc.contributor.authorWang, Zen_HK
dc.contributor.authorCosta, KDen_HK
dc.contributor.authorLi, RAen_HK
dc.contributor.authorSun, Den_HK
dc.date.accessioned2011-10-26T08:43:47Z-
dc.date.available2011-10-26T08:43:47Z-
dc.date.issued2011en_HK
dc.identifier.citationLab On A Chip - Miniaturisation For Chemistry And Biology, 2011, v. 11 n. 21, p. 3656-3662en_HK
dc.identifier.issn1473-0197en_HK
dc.identifier.urihttp://hdl.handle.net/10722/142250-
dc.description.abstractSorting (or isolation) and manipulation of rare cells with high recovery rate and purity are of critical importance to a wide range of physiological applications. In the current paper, we report on a generic single cell manipulation tool that integrates optical tweezers and microfluidic chip technologies for handling small cell population sorting with high accuracy. The laminar flow nature of microfluidics enables the targeted cells to be focused on a desired area for cell isolation. To recognize the target cells, we develop an image processing methodology with a recognition capability of multiple features, e.g., cell size and fluorescence label. The target cells can be moved precisely by optical tweezers to the desired destination in a noninvasive manner. The unique advantages of this sorter are its high recovery rate and purity in small cell population sorting. The design is based on dynamic fluid and dynamic light pattern, in which single as well as multiple laser traps are employed for cell transportation, and a recognition capability of multiple cell features. Experiments of sorting yeast cells and human embryonic stem cells are performed to demonstrate the effectiveness of the proposed cell sorting approach. © The Royal Society of Chemistry 2011.en_HK
dc.languageeng-
dc.publisherRoyal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/locen_HK
dc.relation.ispartofLab on a Chip - Miniaturisation for Chemistry and Biologyen_HK
dc.titleEnhanced cell sorting and manipulation with combined optical tweezer and microfluidic chip technologiesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1473-0197&volume=11&issue=21&spage=3656&epage=3662&date=2011&atitle=Enhanced+cell+sorting+and+manipulation+with+combined+optical+tweezer+and+microfluidic+chip+technologies-
dc.identifier.emailKong, M:marcokong@hku.hken_HK
dc.identifier.emailLi, RA:ronaldli@hkucc.hku.hken_HK
dc.identifier.authorityKong, M=rp01563en_HK
dc.identifier.authorityLi, RA=rp01352en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1039/c1lc20653ben_HK
dc.identifier.pmid21918752-
dc.identifier.scopuseid_2-s2.0-80054031493en_HK
dc.identifier.hkuros197115-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80054031493&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume11en_HK
dc.identifier.issue21en_HK
dc.identifier.spage3656en_HK
dc.identifier.epage3662en_HK
dc.identifier.eissn1473-0189-
dc.identifier.isiWOS:000295732500012-
dc.publisher.placeUnited Kingdomen_HK
dc.relation.projectCell-based Heart Regeneration-
dc.identifier.scopusauthoridWang, X=37014128500en_HK
dc.identifier.scopusauthoridChen, S=53986175500en_HK
dc.identifier.scopusauthoridKong, M=36784634200en_HK
dc.identifier.scopusauthoridWang, Z=15027868200en_HK
dc.identifier.scopusauthoridCosta, KD=26638724700en_HK
dc.identifier.scopusauthoridLi, RA=7404724466en_HK
dc.identifier.scopusauthoridSun, D=7403968591en_HK
dc.identifier.citeulike10017467-

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