File Download
 
 
Supplementary

Conference Paper: Influenza Pseudotyped lentivirus: A flexible useful tool for influenza research
  • Basic View
  • Metadata View
  • XML View
TitleInfluenza Pseudotyped lentivirus: A flexible useful tool for influenza research
 
AuthorsGarcia, J
Buchy, P
Cavailler, P
de Jong, M
Peiris, JSM
 
Issue Date2011
 
PublisherInstitut Pasteur International Network.
 
CitationThe International Symposium on 'Surveillance and Discovery in Respiratory and Other Emerging Infectious Diseases', Phnom Penh, Cambodia, 29-31 May 2011. In the Program of the International Symposium on 'Surveillance and Discovery in Respiratory and Other Emerging Infectious Diseases', 2011, p. 42, abstract no. O17 [How to Cite?]
 
AbstractBackground: Influenza envelop, hemagglutinin (HA), can be incorporated on the surface of replication-deficient lentiviral particles by a process call pseudotyping (reviewed recently in [1]). These chimerical viral particles can be used to investigate HA-related biological processes such as the receptor binding or the identification of HA-specific antibodies in neutralizationbased serological assays. In this presentation, we will show, in the case of H5 from highly pathogenic avian influenza H5N1, how such particles (H5pp) can be produced and used for serodiagnostics of H5N1 safely in Biosafety Level 2 conditions. Results: After biochemical and functional characterizations of these H5-pseudoparticles (H5pp) [2], H5pp serological assay has been thoroughly optimized and validated [3]. During the evaluation of the assay with paired-sera from seasonal and H5N1 pandemic flu vaccines, we could show, thanks to the increased sensitivity of H5pp assay compared with the conventional microneutralization, that the induction of heterosubtypic neutralizing antibodies in some healthy volunteers unexposed to H5N1 mediated by cross-reaction to the H5 HA [4]. The assay we also used to investigate the kinetics of anti-H5 neutralizing antibodies in naturally H5N1- infected patients with severe clinical illness or asymptomatic infection. We showed that people with asymptomatic H5N1 infection have lower H5N1 antibody titers compared to those with severe illness and that in many asymptomatically infected patients the antibody titer decreased to levels below the threshold of positivity within one year [5]. In another study, H5pp assay was used in field investigation of H5N1 transmission in rural Cambodia and showed for example that bathing or swimming in the community ponds was associated to higher risk of H5N1 infection to human [6]. Finally, as H5pp can mimic the presentation of HA at surface of influenza virus, H5pp were used to explore at atomic level the receptor-virus interactions without the potential confounding sialidase activity from the neuraminidase (proof-of-concept can be found in [7]). Conclusion: Through divers examples, we have illustrated the flexibility of influenza pseudotyped lentiviral particles technology that makes it a kind of “Swiss army knife” for flu virologists.
 
DescriptionSession 5: Challenges to confront infectious diseases: information sharing, molecular virology and new technologies
Oral Presentation
The Program can be viewed at: http://pasteur-network-meeting.org/KH2011/Downloads/PROGRAMME_FINAL.pdf
 
DC FieldValue
dc.contributor.authorGarcia, J
 
dc.contributor.authorBuchy, P
 
dc.contributor.authorCavailler, P
 
dc.contributor.authorde Jong, M
 
dc.contributor.authorPeiris, JSM
 
dc.date.accessioned2011-09-23T06:28:27Z
 
dc.date.available2011-09-23T06:28:27Z
 
dc.date.issued2011
 
dc.description.abstractBackground: Influenza envelop, hemagglutinin (HA), can be incorporated on the surface of replication-deficient lentiviral particles by a process call pseudotyping (reviewed recently in [1]). These chimerical viral particles can be used to investigate HA-related biological processes such as the receptor binding or the identification of HA-specific antibodies in neutralizationbased serological assays. In this presentation, we will show, in the case of H5 from highly pathogenic avian influenza H5N1, how such particles (H5pp) can be produced and used for serodiagnostics of H5N1 safely in Biosafety Level 2 conditions. Results: After biochemical and functional characterizations of these H5-pseudoparticles (H5pp) [2], H5pp serological assay has been thoroughly optimized and validated [3]. During the evaluation of the assay with paired-sera from seasonal and H5N1 pandemic flu vaccines, we could show, thanks to the increased sensitivity of H5pp assay compared with the conventional microneutralization, that the induction of heterosubtypic neutralizing antibodies in some healthy volunteers unexposed to H5N1 mediated by cross-reaction to the H5 HA [4]. The assay we also used to investigate the kinetics of anti-H5 neutralizing antibodies in naturally H5N1- infected patients with severe clinical illness or asymptomatic infection. We showed that people with asymptomatic H5N1 infection have lower H5N1 antibody titers compared to those with severe illness and that in many asymptomatically infected patients the antibody titer decreased to levels below the threshold of positivity within one year [5]. In another study, H5pp assay was used in field investigation of H5N1 transmission in rural Cambodia and showed for example that bathing or swimming in the community ponds was associated to higher risk of H5N1 infection to human [6]. Finally, as H5pp can mimic the presentation of HA at surface of influenza virus, H5pp were used to explore at atomic level the receptor-virus interactions without the potential confounding sialidase activity from the neuraminidase (proof-of-concept can be found in [7]). Conclusion: Through divers examples, we have illustrated the flexibility of influenza pseudotyped lentiviral particles technology that makes it a kind of “Swiss army knife” for flu virologists.
 
dc.descriptionSession 5: Challenges to confront infectious diseases: information sharing, molecular virology and new technologies
 
dc.descriptionOral Presentation
 
dc.descriptionThe Program can be viewed at: http://pasteur-network-meeting.org/KH2011/Downloads/PROGRAMME_FINAL.pdf
 
dc.identifier.citationThe International Symposium on 'Surveillance and Discovery in Respiratory and Other Emerging Infectious Diseases', Phnom Penh, Cambodia, 29-31 May 2011. In the Program of the International Symposium on 'Surveillance and Discovery in Respiratory and Other Emerging Infectious Diseases', 2011, p. 42, abstract no. O17 [How to Cite?]
 
dc.identifier.epage42, abstract no. O17
 
dc.identifier.hkuros194091
 
dc.identifier.spage42, abstract no. O17
 
dc.identifier.urihttp://hdl.handle.net/10722/141233
 
dc.languageeng
 
dc.publisherInstitut Pasteur International Network.
 
dc.relation.ispartofThe International Symposium on 'Surveillance and Discovery in Respiratory and Other Emerging Infectious Diseases'
 
dc.titleInfluenza Pseudotyped lentivirus: A flexible useful tool for influenza research
 
dc.typeConference_Paper
 
<?xml encoding="utf-8" version="1.0"?>
<item><contributor.author>Garcia, J</contributor.author>
<contributor.author>Buchy, P</contributor.author>
<contributor.author>Cavailler, P</contributor.author>
<contributor.author>de Jong, M</contributor.author>
<contributor.author>Peiris, JSM</contributor.author>
<date.accessioned>2011-09-23T06:28:27Z</date.accessioned>
<date.available>2011-09-23T06:28:27Z</date.available>
<date.issued>2011</date.issued>
<identifier.citation>The International Symposium on &apos;Surveillance and Discovery in Respiratory and Other Emerging Infectious Diseases&apos;, Phnom Penh, Cambodia, 29-31 May 2011. In the Program of the International Symposium on &apos;Surveillance and Discovery in Respiratory and Other Emerging Infectious Diseases&apos;, 2011, p. 42, abstract no. O17</identifier.citation>
<identifier.uri>http://hdl.handle.net/10722/141233</identifier.uri>
<description>Session 5: Challenges to confront infectious diseases: information sharing, molecular virology and new technologies</description>
<description>Oral Presentation</description>
<description>The Program can be viewed at: http://pasteur-network-meeting.org/KH2011/Downloads/PROGRAMME_FINAL.pdf</description>
<description.abstract>Background: Influenza envelop, hemagglutinin (HA), can be incorporated on the surface of
replication-deficient lentiviral particles by a process call pseudotyping (reviewed recently in
[1]). These chimerical viral particles can be used to investigate HA-related biological processes
such as the receptor binding or the identification of HA-specific antibodies in neutralizationbased
serological assays. In this presentation, we will show, in the case of H5 from highly
pathogenic avian influenza H5N1, how such particles (H5pp) can be produced and used for
serodiagnostics of H5N1 safely in Biosafety Level 2 conditions.
Results: After biochemical and functional characterizations of these H5-pseudoparticles (H5pp)
[2], H5pp serological assay has been thoroughly optimized and validated [3]. During the
evaluation of the assay with paired-sera from seasonal and H5N1 pandemic flu vaccines, we
could show, thanks to the increased sensitivity of H5pp assay compared with the conventional
microneutralization, that the induction of heterosubtypic neutralizing antibodies in some
healthy volunteers unexposed to H5N1 mediated by cross-reaction to the H5 HA [4]. The assay
we also used to investigate the kinetics of anti-H5 neutralizing antibodies in naturally H5N1-
infected patients with severe clinical illness or asymptomatic infection. We showed that people
with asymptomatic H5N1 infection have lower H5N1 antibody titers compared to those with
severe illness and that in many asymptomatically infected patients the antibody titer
decreased to levels below the threshold of positivity within one year [5]. In another study,
H5pp assay was used in field investigation of H5N1 transmission in rural Cambodia and showed
for example that bathing or swimming in the community ponds was associated to higher risk of
H5N1 infection to human [6]. Finally, as H5pp can mimic the presentation of HA at surface of
influenza virus, H5pp were used to explore at atomic level the receptor-virus interactions
without the potential confounding sialidase activity from the neuraminidase (proof-of-concept
can be found in [7]).
Conclusion: Through divers examples, we have illustrated the flexibility of influenza
pseudotyped lentiviral particles technology that makes it a kind of &#8220;Swiss army knife&#8221; for flu
virologists.</description.abstract>
<language>eng</language>
<publisher>Institut Pasteur International Network.</publisher>
<relation.ispartof>The International Symposium on &apos;Surveillance and Discovery in Respiratory and Other Emerging Infectious Diseases&apos;</relation.ispartof>
<title>Influenza Pseudotyped lentivirus: A flexible useful tool for influenza research</title>
<type>Conference_Paper</type>
<identifier.hkuros>194091</identifier.hkuros>
<identifier.spage>42, abstract no. O17</identifier.spage>
<identifier.epage>42, abstract no. O17</identifier.epage>
</item>